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Protein

STE20-like serine/threonine-protein kinase

Gene

Slk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates apoptosis and actin stress fiber dissolution.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631ATPPROSITE-ProRule annotation
Active sitei155 – 1551Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 489ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
STE20-like serine/threonine-protein kinase (EC:2.7.11.1)
Short name:
STE20-like kinase
Short name:
mSLK
Alternative name(s):
Etk4
STE20-related kinase SMAK
STE20-related serine/threonine-protein kinase
Short name:
STE20-related kinase
Serine/threonine-protein kinase 2
Gene namesi
Name:Slk
Synonyms:Kiaa0204, Stk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:103241. Slk.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi436 – 4361D → N: No change in caspase-3-induced cleavage product. 1 Publication

Chemistry

ChEMBLiCHEMBL2176844.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12331233STE20-like serine/threonine-protein kinasePRO_0000233240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei183 – 1831PhosphothreonineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Modified residuei340 – 3401PhosphoserineBy similarity
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei344 – 3441PhosphoserineCombined sources
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei348 – 3481PhosphoserineCombined sources
Modified residuei354 – 3541PhosphoserineCombined sources
Modified residuei372 – 3721PhosphoserineBy similarity
Modified residuei543 – 5431PhosphoserineCombined sources
Modified residuei561 – 5611PhosphoserineBy similarity
Modified residuei566 – 5661PhosphoserineBy similarity
Modified residuei643 – 6431PhosphoserineCombined sources
Modified residuei647 – 6471PhosphoserineCombined sources
Modified residuei666 – 6661PhosphoserineCombined sources
Modified residuei775 – 7751PhosphoserineBy similarity
Modified residuei777 – 7771PhosphoserineCombined sources
Modified residuei812 – 8121PhosphothreonineCombined sources
Modified residuei816 – 8161PhosphoserineCombined sources
Modified residuei1095 – 10951PhosphothreonineBy similarity

Post-translational modificationi

Proteolytically cleaved by caspase-3.1 Publication
Autophosphorylated.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei436 – 4372Cleavage; by caspase-3Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54988.
PaxDbiO54988.
PeptideAtlasiO54988.
PRIDEiO54988.

PTM databases

iPTMnetiO54988.
PhosphoSiteiO54988.

Miscellaneous databases

PMAP-CutDBO54988.

Expressioni

Tissue specificityi

Ubiquitously expressed.3 Publications

Developmental stagei

Ubiquitously expressed from day 7 to 17 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000025060.
CleanExiMM_SLK.
ExpressionAtlasiO54988. baseline and differential.
GenevisibleiO54988. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiO54988. 1 interaction.
MINTiMINT-4105110.
STRINGi10090.ENSMUSP00000049977.

Structurei

3D structure databases

ProteinModelPortaliO54988.
SMRiO54988. Positions 21-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 292259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini873 – 90836UVRPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili824 – 1067244Sequence analysisAdd
BLAST
Coiled coili1107 – 118175Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi16 – 194Poly-Lys
Compositional biasi302 – 681380Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UVR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
GeneTreeiENSGT00810000125351.
HOGENOMiHOG000236268.
HOVERGENiHBG052712.
InParanoidiO54988.
KOiK08836.
OMAiDQEKQQM.
OrthoDBiEOG091G01Y1.
PhylomeDBiO54988.
TreeFamiTF351445.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54988-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFFNFRKIF KLGSEKKKKQ YEHVKRDLNP EEFWEIIGEL GDGAFGKVYK
60 70 80 90 100
AQNKETNVLA AAKVIDTKSE EELEDYMVEI DILASCDHPN IVKLLDAFYY
110 120 130 140 150
ENNLWILIEF CAGGAVDAVM LELERPLTES QIQVVCKQTL EALNYLHDNK
160 170 180 190 200
IIHRDLKAGN ILFTLDGDIK LADFGVSAKN TRTIQRRDSF IGTPYWMAPE
210 220 230 240 250
VVMCETSKDR PYDYKADVWS LGITLIEMAE IEPPHHELNP MRVLLKIAKS
260 270 280 290 300
EPPTLAQPSK WSSNFKDFLR KCLEKNVDAR WTTSQLLQHP FVTVDSNKPV
310 320 330 340 350
RELIAEAKAE VTEEVEDGKE EDEEEEAENA LPIPANKRAS SDLSIASSEE
360 370 380 390 400
DKLSQNACIL ESVSERTEQS TSEDKFSNKI LNEKPTTDGP EKAVDEHASD
410 420 430 440 450
VNLETGAELN DQTVGIHENG REKKRPKLEN LPDTQDQQTV DVNSVSEENE
460 470 480 490 500
NNRVTLETNT DCLKPEEDRN KENQETLESK LIQSEEINDT HIQTMDLVSQ
510 520 530 540 550
ETGEKEADFQ AVDNEVGLTK EETQEKLGKD GTAQKVITSD RSSEVGTDEA
560 570 580 590 600
LDDTQKAAEL SKAAQSGEGD EALAPTQTLA EKPTEGPEAG GAEEEPPGGE
610 620 630 640 650
RVEDKQPEQQ PAVCEAEGQL TSTSETTRAT LEQPETDEVE QVSESNSIEE
660 670 680 690 700
LERLVVTGAE ARALGSEGEA AATEVDLERK ENAQKVPVKA ESQAPAASQP
710 720 730 740 750
SEPHPVLIPS ININSETTEN KEEMGALPKP ETILPPEPEH EKGNDTDSGT
760 770 780 790 800
GSTVENSSGD LNLSISSFLS KAKDSGSVSL QETRRQKKTL KKTRKFIVDG
810 820 830 840 850
VEVSVTTSKI VTDSDSKTEE LRFLRRQELR ELRLLQKEEQ RAQQQLNGKL
860 870 880 890 900
QQQREQIFRR FEQEMLSKKR QYDQEIENLE KQQKQTIERL EQEHTNRLRD
910 920 930 940 950
EAKRIKGEQE KELSKFQNVL KNRKKEVMNE VEKAPRELRR ELTKRRKEEL
960 970 980 990 1000
AQSQHAQEQE FVQKQQQELD GSLKKIIQQQ KAELANIERE CLNNKQQLMR
1010 1020 1030 1040 1050
AREAAIWELE ERHLQEKHQL LKQQLKDQYF MQRHQLLKRH EKETEQMQRY
1060 1070 1080 1090 1100
NQRLIEELKN RQTQERARLP KIQRSEAKTR MAMFKKSLRI NSTATPDQDR
1110 1120 1130 1140 1150
EKIKQFAAQE EKRQKNERMA QHQKHESQMR DLQLQCEANV RELHQLQNEK
1160 1170 1180 1190 1200
CHLLVEHETQ KLKELDEEHS QELKEWREKL RPRKKTLEEE FARKLQEQEV
1210 1220 1230
FFKMTGESEC LNPSAQSRIS KFYPIPTLHS TGS
Length:1,233
Mass (Da):141,457
Last modified:May 2, 2006 - v2
Checksum:iB1A5230666C58A73
GO
Isoform 2 (identifier: O54988-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     927-957: Missing.

Show »
Length:1,202
Mass (Da):137,713
Checksum:i9F4C78A4C31F4AB2
GO

Sequence cautioni

The sequence BAC65500 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641T → N (PubMed:8346215).Curated
Sequence conflicti574 – 5741A → V in AAB96682 (PubMed:9808774).Curated
Sequence conflicti1098 – 10981Q → K in BAC29874 (PubMed:10611247).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei927 – 95731Missing in isoform 2. 2 PublicationsVSP_018101Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039574 mRNA. Translation: AAB96682.1.
AF112855 mRNA. Translation: AAD28717.1.
AK037798 mRNA. Translation: BAC29874.1.
AK122218 mRNA. Translation: BAC65500.1. Different initiation.
BC131675 mRNA. Translation: AAI31676.1.
AK029491 mRNA. Translation: BAC26474.1.
CCDSiCCDS38017.1. [O54988-1]
CCDS50465.1. [O54988-2]
PIRiT14157.
RefSeqiNP_001158111.1. NM_001164639.1. [O54988-2]
NP_033315.2. NM_009289.3. [O54988-1]
UniGeneiMm.281011.

Genome annotation databases

EnsembliENSMUST00000026043; ENSMUSP00000026043; ENSMUSG00000025060. [O54988-2]
ENSMUST00000051691; ENSMUSP00000049977; ENSMUSG00000025060. [O54988-1]
GeneIDi20874.
KEGGimmu:20874.
UCSCiuc008hvf.2. mouse. [O54988-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039574 mRNA. Translation: AAB96682.1.
AF112855 mRNA. Translation: AAD28717.1.
AK037798 mRNA. Translation: BAC29874.1.
AK122218 mRNA. Translation: BAC65500.1. Different initiation.
BC131675 mRNA. Translation: AAI31676.1.
AK029491 mRNA. Translation: BAC26474.1.
CCDSiCCDS38017.1. [O54988-1]
CCDS50465.1. [O54988-2]
PIRiT14157.
RefSeqiNP_001158111.1. NM_001164639.1. [O54988-2]
NP_033315.2. NM_009289.3. [O54988-1]
UniGeneiMm.281011.

3D structure databases

ProteinModelPortaliO54988.
SMRiO54988. Positions 21-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54988. 1 interaction.
MINTiMINT-4105110.
STRINGi10090.ENSMUSP00000049977.

Chemistry

ChEMBLiCHEMBL2176844.

PTM databases

iPTMnetiO54988.
PhosphoSiteiO54988.

Proteomic databases

MaxQBiO54988.
PaxDbiO54988.
PeptideAtlasiO54988.
PRIDEiO54988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026043; ENSMUSP00000026043; ENSMUSG00000025060. [O54988-2]
ENSMUST00000051691; ENSMUSP00000049977; ENSMUSG00000025060. [O54988-1]
GeneIDi20874.
KEGGimmu:20874.
UCSCiuc008hvf.2. mouse. [O54988-1]

Organism-specific databases

CTDi9748.
MGIiMGI:103241. Slk.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
GeneTreeiENSGT00810000125351.
HOGENOMiHOG000236268.
HOVERGENiHBG052712.
InParanoidiO54988.
KOiK08836.
OMAiDQEKQQM.
OrthoDBiEOG091G01Y1.
PhylomeDBiO54988.
TreeFamiTF351445.

Miscellaneous databases

PMAP-CutDBO54988.
PROiO54988.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025060.
CleanExiMM_SLK.
ExpressionAtlasiO54988. baseline and differential.
GenevisibleiO54988. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLK_MOUSE
AccessioniPrimary (citable) accession number: O54988
Secondary accession number(s): A2RRK4
, Q80U65, Q8CAU2, Q8CDW2, Q9WU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.