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Protein

ATPase Asna1

Gene

Asna1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74UniRule annotation1
Binding sitei251ATPUniRule annotation1
Binding sitei278ATPUniRule annotation1
Metal bindingi289Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi292Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-381038. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase Asna1UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Gene namesi
Name:Asna1
Synonyms:Arsa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1928379. Asna1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes early embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001522542 – 348ATPase Asna1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO54984.
MaxQBiO54984.
PaxDbiO54984.
PeptideAtlasiO54984.
PRIDEiO54984.

2D gel databases

REPRODUCTION-2DPAGEiO54984.

PTM databases

iPTMnetiO54984.
PhosphoSitePlusiO54984.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052456.
CleanExiMM_ARSA.
ExpressionAtlasiO54984. baseline and differential.
GenevisibleiO54984. MM.

Interactioni

Subunit structurei

Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with SERP1 and SEC61B. Interacts with WRB.UniRule annotation

Protein-protein interaction databases

BioGridi208019. 8 interactors.
IntActiO54984. 6 interactors.
MINTiMINT-4088407.
STRINGi10090.ENSMUSP00000065337.

Structurei

3D structure databases

ProteinModelPortaliO54984.
SMRiO54984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2825. Eukaryota.
COG0003. LUCA.
GeneTreeiENSGT00390000003817.
HOGENOMiHOG000197637.
InParanoidiO54984.
KOiK01551.
OMAiDQIADLY.
OrthoDBiEOG091G0CAR.
PhylomeDBiO54984.
TreeFamiTF300670.

Family and domain databases

CDDicd02035. ArsA. 1 hit.
HAMAPiMF_03112. Asna1_Get3. 1 hit.
InterProiView protein in InterPro
IPR025723. Anion-transp_ATPase-like_dom.
IPR016300. ATPase_ArsA/GET3.
IPR027542. ATPase_ArsA/GET3_euk.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10803. PTHR10803. 1 hit.
PTHR10803:SF3. PTHR10803:SF3. 1 hit.
PfamiView protein in Pfam
PF02374. ArsA_ATPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00345. GET3_arsA_TRC40. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK
60 70 80 90 100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL
110 120 130 140 150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE
160 170 180 190 200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF
210 220 230 240 250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA
260 270 280 290 300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA
310 320 330 340
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSTQ
Length:348
Mass (Da):38,823
Last modified:July 11, 2001 - v2
Checksum:iDA4EB4ACC35C7A36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039405 mRNA. Translation: AAB94772.2.
AF061177 Genomic DNA. Translation: AAD15826.2.
BC016453 mRNA. Translation: AAH16453.1.
BC083335 mRNA. Translation: AAH83335.1.
AK171690 mRNA. Translation: BAE42614.1.
CH466525 Genomic DNA. Translation: EDL10985.1.
CCDSiCCDS40417.1.
RefSeqiNP_062626.1. NM_019652.1.
UniGeneiMm.41475.

Genome annotation databases

EnsembliENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456.
GeneIDi56495.
KEGGimmu:56495.
UCSCiuc009mox.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiASNA_MOUSE
AccessioniPrimary (citable) accession number: O54984
Secondary accession number(s): Q3TAQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 11, 2001
Last modified: October 25, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families