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O54983

- CRYM_MOUSE

UniProt

O54983 - CRYM_MOUSE

Protein

Ketimine reductase mu-crystallin

Gene

Crym

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors By similarity.By similarity

    Catalytic activityi

    Thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H.

    Cofactori

    NAD or NADP.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei167 – 1671NADPBy similarity
    Binding sitei168 – 1681NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi142 – 1476NADPBy similarity

    GO - Molecular functioni

    1. hormone binding Source: MGI
    2. NADP binding Source: Ensembl
    3. thiomorpholine-carboxylate dehydrogenase activity Source: UniProtKB-EC
    4. thyroid hormone binding Source: Ensembl
    5. transcription corepressor activity Source: Ensembl

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    2. sensory perception of sound Source: Ensembl
    3. thyroid hormone metabolic process Source: MGI
    4. thyroid hormone transport Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ketimine reductase mu-crystallin (EC:1.5.1.25)
    Alternative name(s):
    NADP-regulated thyroid-hormone-binding protein
    Gene namesi
    Name:Crym
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:102675. Crym.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI
    2. nucleus Source: MGI
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Ketimine reductase mu-crystallinPRO_0000200679Add
    BLAST

    Proteomic databases

    MaxQBiO54983.
    PaxDbiO54983.
    PRIDEiO54983.

    PTM databases

    PhosphoSiteiO54983.

    Expressioni

    Gene expression databases

    ArrayExpressiO54983.
    BgeeiO54983.
    CleanExiMM_CRYM.
    GenevestigatoriO54983.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiO54983. 3 interactions.
    MINTiMINT-4092055.
    STRINGi10090.ENSMUSP00000033198.

    Structurei

    Secondary structure

    1
    313
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 83
    Helixi10 – 156
    Helixi20 – 3516
    Helixi37 – 404
    Beta strandi48 – 525
    Helixi53 – 553
    Beta strandi57 – 6610
    Turni67 – 704
    Beta strandi71 – 8010
    Beta strandi91 – 988
    Turni100 – 1023
    Beta strandi105 – 1117
    Helixi112 – 13019
    Beta strandi137 – 1415
    Helixi145 – 15713
    Beta strandi161 – 1666
    Helixi170 – 17910
    Beta strandi180 – 1823
    Beta strandi184 – 1863
    Helixi190 – 1945
    Beta strandi198 – 2025
    Helixi213 – 2153
    Beta strandi221 – 2244
    Helixi237 – 2426
    Beta strandi243 – 2486
    Helixi250 – 2567
    Helixi258 – 2636
    Helixi271 – 2755
    Beta strandi287 – 2904
    Helixi295 – 31117

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BV8X-ray2.30A/B1-313[»]
    4BV9X-ray2.19A/B1-313[»]
    4BVAX-ray1.75A/B1-313[»]
    ProteinModelPortaliO54983.
    SMRiO54983. Positions 2-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ornithine cyclodeaminase family.Curated

    Phylogenomic databases

    eggNOGiCOG2423.
    HOGENOMiHOG000137263.
    HOVERGENiHBG005408.
    InParanoidiO54983.
    KOiK18258.
    OMAiFANEFED.
    OrthoDBiEOG7S7SFB.
    PhylomeDBiO54983.
    TreeFamiTF105309.

    Family and domain databases

    Gene3Di3.30.1780.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR003462. ODC_Mu_crystall.
    IPR023401. ODC_N.
    [Graphical view]
    PfamiPF02423. OCD_Mu_crystall. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001439. CryM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O54983-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRAPAFLSA EEVQDHLRSS SLLIPPLEAA LANFSKGPDG GVMQPVRTVV    50
    PVAKHRGFLG VMPAYSAAED ALTTKLVTFY EGHSNTAVPS HQASVLLFDP 100
    SNGSLLAVMD GNVITAKRTA AVSAIATKLL KPPGSDVLCI LGAGVQAYSH 150
    YEIFTEQFSF KEVRMWNRTR ENAEKFASTV QGDVRVCSSV QEAVTGADVI 200
    ITVTMATEPI LFGEWVKPGA HINAVGASRP DWRELDDELM RQAVLYVDSR 250
    EAALKESGDV LLSGADIFAE LGEVISGAKP AHCEKTTVFK SLGMAVEDLV 300
    AAKLVYDSWS SGK 313
    Length:313
    Mass (Da):33,523
    Last modified:June 1, 1998 - v1
    Checksum:i0898EDA9E2D34C83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039391 mRNA. Translation: AAB94770.1.
    BC045159 mRNA. Translation: AAH45159.1.
    CCDSiCCDS21794.1.
    RefSeqiNP_057878.1. NM_016669.1.
    UniGeneiMm.9114.

    Genome annotation databases

    EnsembliENSMUST00000033198; ENSMUSP00000033198; ENSMUSG00000030905.
    GeneIDi12971.
    KEGGimmu:12971.
    UCSCiuc009jmk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039391 mRNA. Translation: AAB94770.1 .
    BC045159 mRNA. Translation: AAH45159.1 .
    CCDSi CCDS21794.1.
    RefSeqi NP_057878.1. NM_016669.1.
    UniGenei Mm.9114.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BV8 X-ray 2.30 A/B 1-313 [» ]
    4BV9 X-ray 2.19 A/B 1-313 [» ]
    4BVA X-ray 1.75 A/B 1-313 [» ]
    ProteinModelPortali O54983.
    SMRi O54983. Positions 2-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O54983. 3 interactions.
    MINTi MINT-4092055.
    STRINGi 10090.ENSMUSP00000033198.

    PTM databases

    PhosphoSitei O54983.

    Proteomic databases

    MaxQBi O54983.
    PaxDbi O54983.
    PRIDEi O54983.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033198 ; ENSMUSP00000033198 ; ENSMUSG00000030905 .
    GeneIDi 12971.
    KEGGi mmu:12971.
    UCSCi uc009jmk.1. mouse.

    Organism-specific databases

    CTDi 1428.
    MGIi MGI:102675. Crym.

    Phylogenomic databases

    eggNOGi COG2423.
    HOGENOMi HOG000137263.
    HOVERGENi HBG005408.
    InParanoidi O54983.
    KOi K18258.
    OMAi FANEFED.
    OrthoDBi EOG7S7SFB.
    PhylomeDBi O54983.
    TreeFami TF105309.

    Miscellaneous databases

    ChiTaRSi CRYM. mouse.
    NextBioi 282732.
    PROi O54983.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54983.
    Bgeei O54983.
    CleanExi MM_CRYM.
    Genevestigatori O54983.

    Family and domain databases

    Gene3Di 3.30.1780.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR016040. NAD(P)-bd_dom.
    IPR003462. ODC_Mu_crystall.
    IPR023401. ODC_N.
    [Graphical view ]
    Pfami PF02423. OCD_Mu_crystall. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001439. CryM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Sperbeck S.J., Segovia L., Wistow G.J.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: FVB/N.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 4-18; 37-47; 119-128 AND 176-185, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiCRYM_MOUSE
    AccessioniPrimary (citable) accession number: O54983
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3