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O54983 (CRYM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ketimine reductase mu-crystallin

EC=1.5.1.25
Alternative name(s):
NADP-regulated thyroid-hormone-binding protein
Gene names
Name:Crym
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors By similarity.

Catalytic activity

Thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H.

Cofactor

NAD or NADP By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the ornithine cyclodeaminase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Ketimine reductase mu-crystallin
PRO_0000200679

Regions

Nucleotide binding142 – 1476NADP By similarity

Sites

Binding site1671NADP By similarity
Binding site1681NADP By similarity

Secondary structure

....................................................... 313
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O54983 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 0898EDA9E2D34C83

FASTA31333,523
        10         20         30         40         50         60 
MKRAPAFLSA EEVQDHLRSS SLLIPPLEAA LANFSKGPDG GVMQPVRTVV PVAKHRGFLG 

        70         80         90        100        110        120 
VMPAYSAAED ALTTKLVTFY EGHSNTAVPS HQASVLLFDP SNGSLLAVMD GNVITAKRTA 

       130        140        150        160        170        180 
AVSAIATKLL KPPGSDVLCI LGAGVQAYSH YEIFTEQFSF KEVRMWNRTR ENAEKFASTV 

       190        200        210        220        230        240 
QGDVRVCSSV QEAVTGADVI ITVTMATEPI LFGEWVKPGA HINAVGASRP DWRELDDELM 

       250        260        270        280        290        300 
RQAVLYVDSR EAALKESGDV LLSGADIFAE LGEVISGAKP AHCEKTTVFK SLGMAVEDLV 

       310 
AAKLVYDSWS SGK 

« Hide

References

« Hide 'large scale' references
[1]Sperbeck S.J., Segovia L., Wistow G.J.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: FVB/N.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Retina.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 4-18; 37-47; 119-128 AND 176-185, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039391 mRNA. Translation: AAB94770.1.
BC045159 mRNA. Translation: AAH45159.1.
CCDSCCDS21794.1.
RefSeqNP_057878.1. NM_016669.1.
UniGeneMm.9114.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BV8X-ray2.30A/B1-313[»]
4BV9X-ray2.19A/B1-313[»]
4BVAX-ray1.75A/B1-313[»]
ProteinModelPortalO54983.
SMRO54983. Positions 2-312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO54983. 3 interactions.
MINTMINT-4092055.
STRING10090.ENSMUSP00000033198.

PTM databases

PhosphoSiteO54983.

Proteomic databases

MaxQBO54983.
PaxDbO54983.
PRIDEO54983.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033198; ENSMUSP00000033198; ENSMUSG00000030905.
GeneID12971.
KEGGmmu:12971.
UCSCuc009jmk.1. mouse.

Organism-specific databases

CTD1428.
MGIMGI:102675. Crym.

Phylogenomic databases

eggNOGCOG2423.
HOGENOMHOG000137263.
HOVERGENHBG005408.
InParanoidO54983.
KOK18258.
OMAFANEFED.
OrthoDBEOG7S7SFB.
PhylomeDBO54983.
TreeFamTF105309.

Gene expression databases

ArrayExpressO54983.
BgeeO54983.
CleanExMM_CRYM.
GenevestigatorO54983.

Family and domain databases

Gene3D3.30.1780.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR016040. NAD(P)-bd_dom.
IPR003462. ODC_Mu_crystall.
IPR023401. ODC_N.
[Graphical view]
PfamPF02423. OCD_Mu_crystall. 1 hit.
[Graphical view]
PIRSFPIRSF001439. CryM. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCRYM. mouse.
NextBio282732.
PROO54983.
SOURCESearch...

Entry information

Entry nameCRYM_MOUSE
AccessionPrimary (citable) accession number: O54983
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot