O54983 (CRYM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiomorpholine-carboxylate dehydrogenase
EC=1.5.1.25

Alternative name(s):
Mu-crystallin homolog
NADP-regulated thyroid-hormone-binding protein
ketimine reductase

Gene names

Name:

Crym

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors By similarity.
Catalytic activity	Thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H.
Cofactor	NAD or NADP By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ornithine cyclodeaminase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	thyroid hormone metabolic process Inferred from mutant phenotype. Source: MGI
Cellular component	mitochondrion Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	thiomorpholine-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Thiomorpholine-carboxylate dehydrogenase		PRO_0000200679
Regions
	Nucleotide binding	142 – 147	6	NADP By similarity
Sites
	Binding site	167	1	NADP By similarity
	Binding site	168	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54983 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0898EDA9E2D34C83	FASTA	313	33,523
	10 20 30 40 50 60 MKRAPAFLSA EEVQDHLRSS SLLIPPLEAA LANFSKGPDG GVMQPVRTVV PVAKHRGFLG 70 80 90 100 110 120 VMPAYSAAED ALTTKLVTFY EGHSNTAVPS HQASVLLFDP SNGSLLAVMD GNVITAKRTA 130 140 150 160 170 180 AVSAIATKLL KPPGSDVLCI LGAGVQAYSH YEIFTEQFSF KEVRMWNRTR ENAEKFASTV 190 200 210 220 230 240 QGDVRVCSSV QEAVTGADVI ITVTMATEPI LFGEWVKPGA HINAVGASRP DWRELDDELM 250 260 270 280 290 300 RQAVLYVDSR EAALKESGDV LLSGADIFAE LGEVISGAKP AHCEKTTVFK SLGMAVEDLV 310 AAKLVYDSWS SGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sperbeck S.J., Segovia L., Wistow G.J. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FVB/N. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 4-18; 37-47; 119-128 AND 176-185, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039391 mRNA. Translation: AAB94770.1. BC045159 mRNA. Translation: AAH45159.1.
IPI	IPI00120030.
RefSeq	NP_057878.1. NM_016669.1.
UniGene	Mm.9114.
3D structure databases
ProteinModelPortal	O54983.
SMR	O54983. Positions 3-312.
ModBase	Search...
Protein-protein interaction databases
IntAct	O54983. 1 interaction.
STRING	O54983.
Proteomic databases
PRIDE	O54983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033198; ENSMUSP00000033198; ENSMUSG00000030905.
GeneID	12971.
KEGG	mmu:12971.
Organism-specific databases
CTD	1428.
MGI	MGI:102675. Crym.
Phylogenomic databases
eggNOG	roNOG10111.
HOGENOM	HBG300196.
HOVERGEN	HBG005408.
InParanoid	O54983.
OMA	LVTFYED.
OrthoDB	EOG454900.
PhylomeDB	O54983.
Gene expression databases
ArrayExpress	O54983.
Bgee	O54983.
CleanEx	MM_CRYM.
Genevestigator	O54983.
GermOnline	ENSMUSG00000030905. Mus musculus.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR003462. ODC_Mu_crystall. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02423. OCD_Mu_crystall. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282732.
SOURCE	Search...

Entry information

Entry name

CRYM_MOUSE

Accession

Primary (citable) accession number: O54983

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program