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O54982

- KCNU1_MOUSE

UniProt

O54982 - KCNU1_MOUSE

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Protein
Potassium channel subfamily U member 1
Gene
Kcnu1, Kcnma3, Ksper, Slo3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K+. Represents the primary spermatozoan K+ current. In contrast to KCNMA1/SLO1, it is not activated by Ca2+ or Mg2+. Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.8 Publications

GO - Molecular functioni

  1. large conductance calcium-activated potassium channel activity Source: RefGenome
  2. voltage-gated potassium channel activity Source: RefGenome

GO - Biological processi

  1. potassium ion transmembrane transport Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Protein family/group databases

TCDBi1.A.1.3.5. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily U member 1
Alternative name(s):
Calcium-activated potassium channel subunit alpha-3
Calcium-activated potassium channel, subfamily M subunit alpha-3
Pore-forming subunit of the sperm-specific alkalization activated K(+) current
Short name:
KSper
Slowpoke homolog 3
Short name:
mSlo3
pH-sensitive maxi potassium channel
Gene namesi
Name:Kcnu1
Synonyms:Kcnma3, Ksper, Slo3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1202300. Kcnu1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424Extracellular Reviewed prediction
Add
BLAST
Transmembranei25 – 4521Helical; Name=Segment S0; Reviewed prediction
Add
BLAST
Topological domaini46 – 10156Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei102 – 12221Helical; Name=Segment S1; Reviewed prediction
Add
BLAST
Topological domaini123 – 13715Extracellular Reviewed prediction
Add
BLAST
Transmembranei138 – 15821Helical; Name=Segment S2; Reviewed prediction
Add
BLAST
Topological domaini159 – 1657Cytoplasmic Reviewed prediction
Transmembranei166 – 18621Helical; Name=Segment S3; Reviewed prediction
Add
BLAST
Topological domaini187 – 1882Extracellular Reviewed prediction
Transmembranei189 – 20921Helical; Voltage-sensor; Name=Segment S4; Reviewed prediction
Add
BLAST
Topological domaini210 – 22617Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei227 – 24721Helical; Name=Segment S5; Reviewed prediction
Add
BLAST
Topological domaini248 – 25912Extracellular Reviewed prediction
Add
BLAST
Intramembranei260 – 28223Pore-forming; Name=P region; Reviewed prediction
Add
BLAST
Topological domaini283 – 2908Extracellular Reviewed prediction
Transmembranei291 – 31121Helical; Name=Segment S6; Reviewed prediction
Add
BLAST
Topological domaini312 – 1121810Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. voltage-gated potassium channel complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant males are infertile, but their sperm retains some fertility within in vitro fertilization assays. Spermatozoa exhibit a higher incidence of morphological abnormalities as compared to wild-type, accentuated by hypotonic challenge and deficits in motility, in the absence of bicarbonate.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi279 – 2791F → Y: Does not induce any change in single channel conductance or variance in open current levels. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11211121Potassium channel subfamily U member 1
PRO_0000349188Add
BLAST

Proteomic databases

PRIDEiO54982.

Expressioni

Tissue specificityi

Testis-specific. Mainly expressed in spermatocytes.2 Publications

Developmental stagei

Very low expression levels in testis before postnatal day 25 (P25). Levels strongly increase between P25 and P30, and then remain high from P30 through P150.1 Publication

Gene expression databases

GenevestigatoriO54982.

Interactioni

Subunit structurei

Homotetramer; which constitutes the calcium-activated potassium channel. May interact with LRRC52; this interaction may change some channel gating properties, such as shifting gating to more negative potentials at a given pH.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096457.

Structurei

3D structure databases

ProteinModelPortaliO54982.
SMRiO54982. Positions 148-316, 331-600, 686-1048.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 482144RCK N-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni480 – 50021Segment S7
Add
BLAST
Regioni537 – 55721Segment S8
Add
BLAST
Regioni716 – 73621Segment S9
Add
BLAST
Regioni900 – 92021Segment S1
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi276 – 2794Selectivity for potassium

Domaini

The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor By similarity.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium By similarity.
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel By similarity.
The C-terminal cytosolic region confers the pH-dependence.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253173.
HOGENOMiHOG000019856.
HOVERGENiHBG052222.
InParanoidiO54982.
KOiK05274.
PhylomeDBiO54982.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01449. BKCHANNELA.

Sequencei

Sequence statusi: Complete.

O54982-1 [UniParc]FASTAAdd to Basket

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MSQTLLDSLN QKELTETSCT IEIQAAFILS SLATFFGGLI ILFLFRIALK     50
SSRSWKYVKG PRGLLELFSS RRIEANPLRK LYFHGVFRQR IEMLLSAQTV 100
VGQVLVILVF VLSIGSLVIY FINSMDPVRR CSSYEDKIVH GDLSFNAFFS 150
FYFGLRFWAA EDKIKFWLEM NSIVDIFTIP PTFISYYLKS NWLGLRFLRA 200
LRLLELPKIL QILQVIKTSN SVKLSKLLSI VISTWFTAAG FLHLVENSGD 250
PWLNGRNSQT MSYFESIYLV TATMSTVGFG DVVAKTSLGR IFIVFFTLGS 300
LILFANYIPE MVELFSTRKK YTKPYEAVKG KKFIVVCGNI TVDSVTAFLR 350
NFLHWKSGEI NIEIVFLGET LPCLELETLL KCHTSCTNFV CGTALKFEDL 400
KRVAVENSEA CLILANHFCS DLHDEDNSNI MRVLSIKNYY PQTRVIIQIL 450
QSQNKVFLSK IPNWDWSAGD NILCFAELKL GFIAQGCLVP GLCTFLTTLF 500
IEQNQKVFPK HPWQKHFLNG LKNKILTQRL SNDFVGMTFP QVSRLCFVKL 550
NLMLIAIQHK PFFHSCCTLI LNPSSQVRLN KDTLGFFIAD SSKAVKRAFF 600
YCSNCHSDVC NPELIGKCNC KIKSRQQLIA PTIMVMKSSL TDFTTSSHIH 650
ASMSTEIHTC FSREQPSLIT ITTNRPTTND TVDDTDMLDS SGMFHWCRAM 700
PLDKVVLKRS EKAKHEFQNH IVVCVFGDAQ CTLVGLRNFV MPLRASNYTR 750
QELKDIVFIG SLEYFQREWR FLRNFPKIHI MPGSALYMGD LIAVNVEQCS 800
MCVILATPYK ALSSQILVDT EAIMATLNIQ SLRITSPTPG SSKSEVKPSS 850
AFDSKERKQR YKQIPILTEL KNPSNIHFIE QMGGLDGMLK GTSLHLSTSF 900
STGAVFSDTF LDSLLATSFY NYHVVELLQM LVTGGISSEM EHYLVKEKPY 950
KTTDDYEAIK SGRTRCKLGL LSLDQTVLSG INPRKTFGQL FCGSLDNFGI 1000
LCVGLYRMID EEEPSQEHKR FVITRPSNEC HLLPSDLVFC AIPFNTTCGK 1050
SDSSPSIQAQ NNSTNATTPL AQGSNFFDSH HADESHDLYP VDDTGERWSQ 1100
HHHSRVYPLD TLDASDIVQE K 1121
Length:1,121
Mass (Da):126,870
Last modified:March 7, 2006 - v2
Checksum:i4D7E0425B97B47A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039213 mRNA. Translation: AAB99742.2.
CCDSiCCDS52530.1.
PIRiT42383.
RefSeqiNP_032458.3. NM_008432.3.
UniGeneiMm.289679.

Genome annotation databases

GeneIDi16532.
KEGGimmu:16532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039213 mRNA. Translation: AAB99742.2 .
CCDSi CCDS52530.1.
PIRi T42383.
RefSeqi NP_032458.3. NM_008432.3.
UniGenei Mm.289679.

3D structure databases

ProteinModelPortali O54982.
SMRi O54982. Positions 148-316, 331-600, 686-1048.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000096457.

Chemistry

GuidetoPHARMACOLOGYi 387.

Protein family/group databases

TCDBi 1.A.1.3.5. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PRIDEi O54982.

Protocols and materials databases

DNASUi 16532.
Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16532.
KEGGi mmu:16532.

Organism-specific databases

CTDi 157855.
MGIi MGI:1202300. Kcnu1.

Phylogenomic databases

eggNOGi NOG253173.
HOGENOMi HOG000019856.
HOVERGENi HBG052222.
InParanoidi O54982.
KOi K05274.
PhylomeDBi O54982.

Miscellaneous databases

NextBioi 289949.
PROi O54982.
SOURCEi Search...

Gene expression databases

Genevestigatori O54982.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR01449. BKCHANNELA.
ProtoNeti Search...

Publicationsi

  1. "Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes."
    Schreiber M., Wei A., Yuan A., Gaut J., Saito M., Salkoff L.
    J. Biol. Chem. 273:3509-3516(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Intracellular Mg(2+) enhances the function of BK-type Ca(2+)-activated K(+) channels."
    Shi J., Cui J.
    J. Gen. Physiol. 118:589-606(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Gating and conductance properties of BK channels are modulated by the S9-S10 tail domain of the alpha subunit. A study of mSlo1 and mSlo3 wild-type and chimeric channels."
    Moss B.L., Magleby K.L.
    J. Gen. Physiol. 118:711-734(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Ligand-dependent activation of Slo family channels is defined by interchangeable cytosolic domains."
    Xia X.-M., Zhang X., Lingle C.J.
    J. Neurosci. 24:5585-5591(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Slo3 K+ channels: voltage and pH dependence of macroscopic currents."
    Zhang X., Zeng X., Lingle C.J.
    J. Gen. Physiol. 128:317-336(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "pH-regulated Slo3 K+ channels: properties of unitary currents."
    Zhang X., Zeng X., Xia X.-M., Lingle C.J.
    J. Gen. Physiol. 128:301-315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF PHE-279.
  7. "Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa."
    Zeng X.H., Yang C., Kim S.T., Lingle C.J., Xia X.M.
    Proc. Natl. Acad. Sci. U.S.A. 108:5879-5884(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION AS KSPER, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  8. "LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel."
    Yang C., Zeng X.H., Zhou Y., Xia X.M., Lingle C.J.
    Proc. Natl. Acad. Sci. U.S.A. 108:19419-19424(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LRRC52, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  9. "Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel."
    Leonetti M.D., Yuan P., Hsiung Y., Mackinnon R.
    Proc. Natl. Acad. Sci. U.S.A. 109:19274-19279(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PH DEPENDENCE.

Entry informationi

Entry nameiKCNU1_MOUSE
AccessioniPrimary (citable) accession number: O54982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 7, 2006
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi