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Protein

Potassium channel subfamily U member 1

Gene

Kcnu1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K+. Represents the primary spermatozoan K+ current. In contrast to KCNMA1/SLO1, it is not activated by Ca2+ or Mg2+. Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.8 Publications

GO - Molecular functioni

GO - Biological processi

  • potassium ion transport Source: MGI
  • protein stabilization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Protein family/group databases

TCDBi1.A.1.3.5. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily U member 1
Alternative name(s):
Calcium-activated potassium channel subunit alpha-3
Calcium-activated potassium channel, subfamily M subunit alpha-3
Pore-forming subunit of the sperm-specific alkalization activated K(+) current
Short name:
KSper
Slowpoke homolog 3
Short name:
mSlo3
pH-sensitive maxi potassium channel
Gene namesi
Name:Kcnu1
Synonyms:Kcnma3, Ksper, Slo3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1202300. Kcnu1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24ExtracellularSequence analysisAdd BLAST24
Transmembranei25 – 45Helical; Name=Segment S0Sequence analysisAdd BLAST21
Topological domaini46 – 101CytoplasmicSequence analysisAdd BLAST56
Transmembranei102 – 122Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini123 – 137ExtracellularSequence analysisAdd BLAST15
Transmembranei138 – 158Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini159 – 165CytoplasmicSequence analysis7
Transmembranei166 – 186Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini187 – 188ExtracellularSequence analysis2
Transmembranei189 – 209Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini210 – 226CytoplasmicSequence analysisAdd BLAST17
Transmembranei227 – 247Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini248 – 259ExtracellularSequence analysisAdd BLAST12
Intramembranei260 – 282Pore-forming; Name=P regionSequence analysisAdd BLAST23
Topological domaini283 – 290ExtracellularSequence analysis8
Transmembranei291 – 311Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini312 – 1121CytoplasmicSequence analysisAdd BLAST810

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant males are infertile, but their sperm retains some fertility within in vitro fertilization assays. Spermatozoa exhibit a higher incidence of morphological abnormalities as compared to wild-type, accentuated by hypotonic challenge and deficits in motility, in the absence of bicarbonate.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi279F → Y: Does not induce any change in single channel conductance or variance in open current levels. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003491881 – 1121Potassium channel subfamily U member 1Add BLAST1121

Proteomic databases

PaxDbiO54982.
PRIDEiO54982.

Expressioni

Tissue specificityi

Testis-specific. Mainly expressed in spermatocytes.2 Publications

Developmental stagei

Very low expression levels in testis before postnatal day 25 (P25). Levels strongly increase between P25 and P30, and then remain high from P30 through P150.1 Publication

Interactioni

Subunit structurei

Homotetramer; which constitutes the calcium-activated potassium channel. May interact with LRRC52; this interaction may change some channel gating properties, such as shifting gating to more negative potentials at a given pH.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096457.

Structurei

3D structure databases

ProteinModelPortaliO54982.
SMRiO54982.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 482RCK N-terminalAdd BLAST144

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni480 – 500Segment S7Add BLAST21
Regioni537 – 557Segment S8Add BLAST21
Regioni716 – 736Segment S9Add BLAST21
Regioni900 – 920Segment S1Add BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 279Selectivity for potassium4

Domaini

The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.By similarity
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium (By similarity).By similarity
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel.By similarity
The C-terminal cytosolic region confers the pH-dependence.

Sequence similaritiesi

Contains 1 RCK N-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1420. Eukaryota.
ENOG410YUX1. LUCA.
HOGENOMiHOG000019856.
HOVERGENiHBG052222.
InParanoidiO54982.
KOiK05274.
PhylomeDBiO54982.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01449. BKCHANNELA.

Sequencei

Sequence statusi: Complete.

O54982-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTLLDSLN QKELTETSCT IEIQAAFILS SLATFFGGLI ILFLFRIALK
60 70 80 90 100
SSRSWKYVKG PRGLLELFSS RRIEANPLRK LYFHGVFRQR IEMLLSAQTV
110 120 130 140 150
VGQVLVILVF VLSIGSLVIY FINSMDPVRR CSSYEDKIVH GDLSFNAFFS
160 170 180 190 200
FYFGLRFWAA EDKIKFWLEM NSIVDIFTIP PTFISYYLKS NWLGLRFLRA
210 220 230 240 250
LRLLELPKIL QILQVIKTSN SVKLSKLLSI VISTWFTAAG FLHLVENSGD
260 270 280 290 300
PWLNGRNSQT MSYFESIYLV TATMSTVGFG DVVAKTSLGR IFIVFFTLGS
310 320 330 340 350
LILFANYIPE MVELFSTRKK YTKPYEAVKG KKFIVVCGNI TVDSVTAFLR
360 370 380 390 400
NFLHWKSGEI NIEIVFLGET LPCLELETLL KCHTSCTNFV CGTALKFEDL
410 420 430 440 450
KRVAVENSEA CLILANHFCS DLHDEDNSNI MRVLSIKNYY PQTRVIIQIL
460 470 480 490 500
QSQNKVFLSK IPNWDWSAGD NILCFAELKL GFIAQGCLVP GLCTFLTTLF
510 520 530 540 550
IEQNQKVFPK HPWQKHFLNG LKNKILTQRL SNDFVGMTFP QVSRLCFVKL
560 570 580 590 600
NLMLIAIQHK PFFHSCCTLI LNPSSQVRLN KDTLGFFIAD SSKAVKRAFF
610 620 630 640 650
YCSNCHSDVC NPELIGKCNC KIKSRQQLIA PTIMVMKSSL TDFTTSSHIH
660 670 680 690 700
ASMSTEIHTC FSREQPSLIT ITTNRPTTND TVDDTDMLDS SGMFHWCRAM
710 720 730 740 750
PLDKVVLKRS EKAKHEFQNH IVVCVFGDAQ CTLVGLRNFV MPLRASNYTR
760 770 780 790 800
QELKDIVFIG SLEYFQREWR FLRNFPKIHI MPGSALYMGD LIAVNVEQCS
810 820 830 840 850
MCVILATPYK ALSSQILVDT EAIMATLNIQ SLRITSPTPG SSKSEVKPSS
860 870 880 890 900
AFDSKERKQR YKQIPILTEL KNPSNIHFIE QMGGLDGMLK GTSLHLSTSF
910 920 930 940 950
STGAVFSDTF LDSLLATSFY NYHVVELLQM LVTGGISSEM EHYLVKEKPY
960 970 980 990 1000
KTTDDYEAIK SGRTRCKLGL LSLDQTVLSG INPRKTFGQL FCGSLDNFGI
1010 1020 1030 1040 1050
LCVGLYRMID EEEPSQEHKR FVITRPSNEC HLLPSDLVFC AIPFNTTCGK
1060 1070 1080 1090 1100
SDSSPSIQAQ NNSTNATTPL AQGSNFFDSH HADESHDLYP VDDTGERWSQ
1110 1120
HHHSRVYPLD TLDASDIVQE K
Length:1,121
Mass (Da):126,870
Last modified:March 7, 2006 - v2
Checksum:i4D7E0425B97B47A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039213 mRNA. Translation: AAB99742.2.
CCDSiCCDS52530.1.
PIRiT42383.
RefSeqiNP_032458.3. NM_008432.3.
UniGeneiMm.289679.

Genome annotation databases

GeneIDi16532.
KEGGimmu:16532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039213 mRNA. Translation: AAB99742.2.
CCDSiCCDS52530.1.
PIRiT42383.
RefSeqiNP_032458.3. NM_008432.3.
UniGeneiMm.289679.

3D structure databases

ProteinModelPortaliO54982.
SMRiO54982.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096457.

Chemistry databases

GuidetoPHARMACOLOGYi387.

Protein family/group databases

TCDBi1.A.1.3.5. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiO54982.
PRIDEiO54982.

Protocols and materials databases

DNASUi16532.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16532.
KEGGimmu:16532.

Organism-specific databases

CTDi157855.
MGIiMGI:1202300. Kcnu1.

Phylogenomic databases

eggNOGiKOG1420. Eukaryota.
ENOG410YUX1. LUCA.
HOGENOMiHOG000019856.
HOVERGENiHBG052222.
InParanoidiO54982.
KOiK05274.
PhylomeDBiO54982.

Miscellaneous databases

PROiO54982.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01449. BKCHANNELA.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNU1_MOUSE
AccessioniPrimary (citable) accession number: O54982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 7, 2006
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.