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O54975 (XPP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Xaa-Pro aminopeptidase 1
EC=3.4.11.9
Alternative name(s):
Aminoacylproline aminopeptidase
Cytosolic aminopeptidase P
Soluble aminopeptidase P
Short name=sAmp
X-Pro aminopeptidase 1
X-prolyl aminopeptidase 1, soluble

Cleaved into the following chain:

  1. Xaa-Pro aminopeptidase 1, N-terminally processed
Gene names
Name:Xpnpep1
Synonyms:App
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro By similarity.

Catalytic activity

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide. Ref.1

Cofactor

Binds 2 manganese ions per subunit By similarity.

Enzyme regulation

Inhibited by inositol hexakisphosphate. Ref.1

Subunit structure

Homodimer By similarity. UniProtKB Q9NQW7

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Expressed in all tissues tested, including liver, adrenal decapsular tissue, adrenal capsular tissue, corpus luteum, testis, submandibular gland, thymus, brain, cerebellum and heart. Highest levels in testis. Ref.1

Sequence similarities

Belongs to the peptidase M24B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Xaa-Pro aminopeptidase 1
PRO_0000185085
Initiator methionine11Removed; alternate By similarity
Chain2 – 623622Xaa-Pro aminopeptidase 1, N-terminally processed
PRO_0000421792

Sites

Metal binding4151Manganese 1 By similarity
Metal binding4261Manganese 1 By similarity
Metal binding4261Manganese 2 By similarity
Metal binding4891Manganese 2 By similarity
Metal binding5231Manganese 1 By similarity
Metal binding5231Manganese 2 By similarity
Metal binding5371Manganese 1 By similarity
Metal binding5371Manganese 2 By similarity

Amino acid modifications

Modified residue3041N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O54975 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 42D21C2617F88225

FASTA62369,658
        10         20         30         40         50         60 
MAPKVTSELL RQLRQAMRNS ECVAEPIQAY IIPSGDAHQS EYIAPCDCRR AFVSGFDGSA 

        70         80         90        100        110        120 
GTAIITEEHA AMWTDGRYFL QAAKQMDNNW TLMKMGLKDT PTQEDWLVSV LPEGSRVGVD 

       130        140        150        160        170        180 
PLIIPTDYWK KMAKVLRSAG HHLVPVKENL VDKIWTDRPE RPCKPLLTLG LDYTGISWKE 

       190        200        210        220        230        240 
KVADLRLKMA ERSIVWFVVT ALDEIAWLFN LRGSDVEHNP VFFSYAIIGL ERIMLFIDGD 

       250        260        270        280        290        300 
RIDAPGVKQH LLLDLGLEAE YKIQVLPYKS ILSELKTLCA DLSPREKVWV SDKASYAVSE 

       310        320        330        340        350        360 
AIPKDHRCCM PYTPICIAKA VKNSAESAGM RRAHIKDAVA LCELFNWLEQ EVPKGGVTEI 

       370        380        390        400        410        420 
SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPIP ETNRTLSLDE VYLIDSGAQY 

       430        440        450        460        470        480 
KDGTTDVTRT MHFGTPTAYE KECFTYVLKG HIAVSAAVFP TGTKGHLLDS FARSALWDSG 

       490        500        510        520        530        540 
LDYLHGTGHG VGSFLNVHEG PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV 

       550        560        570        580        590        600 
LVVPAKTKYN FNNRGSLTFE PLTLVPIQTK MIDVDALTDK ECDWLNSYHQ TCRDVIGKEL 

       610        620 
QTQGRQEALE WLLRETEPIS RQH

« Hide

References

« Hide 'large scale' references
[1]"Cloning and functional expression of the cytoplasmic form of rat aminopeptidase P."
Czirjak G., Burkhart W.A., Moyer M.B., Antal J., Shears S.B., Enyedi P.
Biochim. Biophys. Acta 1444:326-336(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-19; 85-89; 96-105; 107-112; 372-385; 422-442 AND 599-606, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF038591 mRNA. Translation: AAB95331.1.
BC061758 mRNA. Translation: AAH61758.1.
IPIIPI00781123.
RefSeqNP_571988.1. NM_131913.1.
UniGeneRn.25763.

3D structure databases

ProteinModelPortalO54975.
SMRO54975. Positions 3-619.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000031325.

Protein family/group databases

MEROPSM24.009.

PTM databases

PhosphoSiteO54975.

Proteomic databases

PaxDbO54975.
PRIDEO54975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000033148; ENSRNOP00000031325; ENSRNOG00000012084.
GeneID170751.
KEGGrno:170751.
UCSCRGD:621274. rat.

Organism-specific databases

CTD7511.
RGD621274. Xpnpep1.

Phylogenomic databases

eggNOGCOG0006.
GeneTreeENSGT00390000013970.
HOGENOMHOG000255713.
HOVERGENHBG002934.
InParanoidO54975.
KOK01262.
OrthoDBEOG4BZN23.

Gene expression databases

GenevestigatorO54975.
GermOnlineENSRNOG00000012084. Rattus norvegicus.

Family and domain databases

Gene3D3.90.230.10. 1 hit.
InterProIPR000587. Creatinase.
IPR000994. Pept_M24_structural-domain.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SUPFAMSSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITEPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio621211.

Entry information

Entry nameXPP1_RAT
AccessionPrimary (citable) accession number: O54975
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 1998
Last modified: April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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