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Protein

Xaa-Pro aminopeptidase 1

Gene

Xpnpep1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity).By similarity

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by inositol hexakisphosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi415 – 4151Manganese 1By similarity
Metal bindingi426 – 4261Manganese 1By similarity
Metal bindingi426 – 4261Manganese 2By similarity
Metal bindingi489 – 4891Manganese 2By similarity
Metal bindingi523 – 5231Manganese 1By similarity
Metal bindingi523 – 5231Manganese 2By similarity
Metal bindingi537 – 5371Manganese 1By similarity
Metal bindingi537 – 5371Manganese 2By similarity

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • manganese ion binding Source: UniProtKB
  • metalloaminopeptidase activity Source: UniProtKB

GO - Biological processi

  • bradykinin catabolic process Source: UniProtKB
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM24.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase 1 (EC:3.4.11.9)
Alternative name(s):
Aminoacylproline aminopeptidase
Cytosolic aminopeptidase P
Soluble aminopeptidase P
Short name:
sAmp
X-Pro aminopeptidase 1
X-prolyl aminopeptidase 1, soluble
Cleaved into the following chain:
Gene namesi
Name:Xpnpep1Imported
Synonyms:App
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621274. Xpnpep1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Xaa-Pro aminopeptidase 1PRO_0000185085Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 623622Xaa-Pro aminopeptidase 1, N-terminally processedPRO_0000421792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO54975.
PRIDEiO54975.

PTM databases

iPTMnetiO54975.
PhosphoSiteiO54975.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including liver, adrenal decapsular tissue, adrenal capsular tissue, corpus luteum, testis, submandibular gland, thymus, brain, cerebellum and heart. Highest levels in testis.1 Publication

Gene expression databases

ExpressionAtlasiO54975. baseline and differential.
GenevisibleiO54975. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi250985. 2 interactions.
STRINGi10116.ENSRNOP00000031325.

Structurei

3D structure databases

ProteinModelPortaliO54975.
SMRiO54975. Positions 3-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Sequence analysis

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiO54975.
KOiK01262.
OMAiYRPGKWG.
OrthoDBiEOG7BGHK4.
PhylomeDBiO54975.

Family and domain databases

Gene3Di3.40.350.10. 2 hits.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKVTSELL RQLRQAMRNS ECVAEPIQAY IIPSGDAHQS EYIAPCDCRR
60 70 80 90 100
AFVSGFDGSA GTAIITEEHA AMWTDGRYFL QAAKQMDNNW TLMKMGLKDT
110 120 130 140 150
PTQEDWLVSV LPEGSRVGVD PLIIPTDYWK KMAKVLRSAG HHLVPVKENL
160 170 180 190 200
VDKIWTDRPE RPCKPLLTLG LDYTGISWKE KVADLRLKMA ERSIVWFVVT
210 220 230 240 250
ALDEIAWLFN LRGSDVEHNP VFFSYAIIGL ERIMLFIDGD RIDAPGVKQH
260 270 280 290 300
LLLDLGLEAE YKIQVLPYKS ILSELKTLCA DLSPREKVWV SDKASYAVSE
310 320 330 340 350
AIPKDHRCCM PYTPICIAKA VKNSAESAGM RRAHIKDAVA LCELFNWLEQ
360 370 380 390 400
EVPKGGVTEI SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPIP
410 420 430 440 450
ETNRTLSLDE VYLIDSGAQY KDGTTDVTRT MHFGTPTAYE KECFTYVLKG
460 470 480 490 500
HIAVSAAVFP TGTKGHLLDS FARSALWDSG LDYLHGTGHG VGSFLNVHEG
510 520 530 540 550
PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV LVVPAKTKYN
560 570 580 590 600
FNNRGSLTFE PLTLVPIQTK MIDVDALTDK ECDWLNSYHQ TCRDVIGKEL
610 620
QTQGRQEALE WLLRETEPIS RQH
Length:623
Mass (Da):69,658
Last modified:June 1, 1998 - v1
Checksum:i42D21C2617F88225
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038591 mRNA. Translation: AAB95331.1.
BC061758 mRNA. Translation: AAH61758.1.
RefSeqiNP_571988.1. NM_131913.1.
UniGeneiRn.25763.

Genome annotation databases

EnsembliENSRNOT00000033148; ENSRNOP00000031325; ENSRNOG00000012084.
GeneIDi170751.
KEGGirno:170751.
UCSCiRGD:621274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038591 mRNA. Translation: AAB95331.1.
BC061758 mRNA. Translation: AAH61758.1.
RefSeqiNP_571988.1. NM_131913.1.
UniGeneiRn.25763.

3D structure databases

ProteinModelPortaliO54975.
SMRiO54975. Positions 3-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250985. 2 interactions.
STRINGi10116.ENSRNOP00000031325.

Protein family/group databases

MEROPSiM24.009.

PTM databases

iPTMnetiO54975.
PhosphoSiteiO54975.

Proteomic databases

PaxDbiO54975.
PRIDEiO54975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033148; ENSRNOP00000031325; ENSRNOG00000012084.
GeneIDi170751.
KEGGirno:170751.
UCSCiRGD:621274. rat.

Organism-specific databases

CTDi7511.
RGDi621274. Xpnpep1.

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
GeneTreeiENSGT00390000013970.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiO54975.
KOiK01262.
OMAiYRPGKWG.
OrthoDBiEOG7BGHK4.
PhylomeDBiO54975.

Miscellaneous databases

PROiO54975.

Gene expression databases

ExpressionAtlasiO54975. baseline and differential.
GenevisibleiO54975. RN.

Family and domain databases

Gene3Di3.40.350.10. 2 hits.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional expression of the cytoplasmic form of rat aminopeptidase P."
    Czirjak G., Burkhart W.A., Moyer M.B., Antal J., Shears S.B., Enyedi P.
    Biochim. Biophys. Acta 1444:326-336(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-19; 85-89; 96-105; 107-112; 372-385; 422-442 AND 599-606, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: LiverImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiXPP1_RAT
AccessioniPrimary (citable) accession number: O54975
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.