Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein CBFA2T3

Gene

Cbfa2t3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri533 – 569MYND-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CBFA2T3
Alternative name(s):
Eight twenty one protein 2
MTG8-related protein 2
Protein ETO-2
Gene namesi
Name:Cbfa2t3
Synonyms:Cbfa2t3h, Mtgr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1338013 Cbfa2t3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071741 – 620Protein CBFA2T3Add BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei603PhosphoserineBy similarity1
Modified residuei607PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54972
PaxDbiO54972
PRIDEiO54972

PTM databases

iPTMnetiO54972
PhosphoSitePlusiO54972

Expressioni

Tissue specificityi

Widely expressed with higher expression in heart, brain, lung and spleen. Expressed in hematopoietic cells (at protein level).2 Publications

Developmental stagei

Expressed during embryogenesis from 7 to 17 dpc.1 Publication

Inductioni

Up-regulated during hematopoietic differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000006362
ExpressionAtlasiO54972 baseline and differential
GenevisibleiO54972 MM

Interactioni

Subunit structurei

Heterodimer with RUNX1T1 and CBFA2T2. Interacts with ZNF652 and ERBB4. May interact with PRKAR2A, PDE7A and probably PDE4A. Interacts with PLXNA1, PLXNA3 and PRKAR1A (By similarity). Interacts with HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, NCOR1 and NCOR2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with ZBTB4, ZBTB38 and ZBTB33. Interacts with HIF1A and EGLN1 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi198523, 2 interactors
IntActiO54972, 5 interactors
MINTiO54972
STRINGi10090.ENSMUSP00000118997

Structurei

3D structure databases

ProteinModelPortaliO54972
SMRiO54972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 241TAFHPROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 412Mediates localization to the nucleusAdd BLAST412
Regioni120 – 217Interaction with ZBTB33By similarityAdd BLAST98
Regioni151 – 243Interaction with HIF1ABy similarityAdd BLAST93
Regioni371 – 389Nervy homology region 2 (NHR2); essential for down-regulation of PFKFB3, PFKFB4 and PDK1 expressionBy similarityAdd BLAST19
Regioni462 – 510Nervy homology region 3 (NHR3); essential for down-regulation of PFKFB3, PFKFB4 and PDK1 expressionBy similarityAdd BLAST49
Regioni462 – 483Mediates interaction with PRKAR2ABy similarityAdd BLAST22

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili465 – 520Sequence analysisAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi37 – 73Pro-richAdd BLAST37

Domaini

Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure.By similarity

Sequence similaritiesi

Belongs to the CBFA2T family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri533 – 569MYND-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IG1Z Eukaryota
ENOG410XR30 LUCA
GeneTreeiENSGT00390000013479
HOGENOMiHOG000049245
HOVERGENiHBG000169
InParanoidiO54972

Family and domain databases

Gene3Di1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR013289 CBFA2T1/2/3
IPR013292 CBFA2T3
IPR014896 NHR2
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
IPR002893 Znf_MYND
PANTHERiPTHR10379 PTHR10379, 1 hit
PfamiView protein in Pfam
PF08788 NHR2, 1 hit
PF07531 TAFH, 1 hit
PF01753 zf-MYND, 1 hit
PRINTSiPR01875 ETOFAMILY
PR01878 MTG16PROTEIN
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit
PS01360 ZF_MYND_1, 1 hit
PS50865 ZF_MYND_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54972-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQASTTTLE SGALLSGPRG LQYGSPAHRK EKAAAMPDSP AEVKTQPRST
60 70 80 90 100
PPSMPPPPPT SSQGATRPPS FTPHTHGEDG PATSLPHGRF HGCLKWSMVC
110 120 130 140 150
LLMNGSSHSP TAIHGAPSTP NGFSNGPATS STASLSTQHL PPACGARQLS
160 170 180 190 200
KLKRFLTTLQ QFGSDISPEI GERVRTLVLG LVNSTLTIEE FHAKLQEATN
210 220 230 240 250
FPLRPFVIPF LKANLPLLQR ELLHCARLAK QTPAQYLAQH EQLLLDASAT
260 270 280 290 300
SPVDSSELLL EVNENGKRRT PDRTKENGSD RDPLHPDHLS KRSCTLSPAQ
310 320 330 340 350
RCSPSNGLPH PTPPPPPHYR LEDMAMAHHF RDSYRHPDPR ELRERHRPLA
360 370 380 390 400
IPGSRQEEVI DHRLTEREWA EEWKHLNSLL NCIMDMVEKT RRSLTVLRRC
410 420 430 440 450
QEADREELNH WIRCYSDSEE GKKGPTPISA RSLNSCSGPE GSQLDVHRDF
460 470 480 490 500
TPRTLSGYMP EEIWRKAEEA VNEVKRQAMS ELQKAVSDAE RKAHELITTE
510 520 530 540 550
RAKMERALAE AKRQASEDAL TVINQQEDSS ESCWNCGRKA SETCSGCNAA
560 570 580 590 600
RYCGSFCQHK DWEKHHHVCG QSLQGPAAAV ADPLPGQPDA TASPSEAGSA
610 620
GPSRPCSPGP PGPLDAAVPR
Length:620
Mass (Da):68,032
Last modified:October 23, 2007 - v2
Checksum:i78DC1995BE365E43
GO
Isoform 2 (identifier: O54972-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     76-101: Missing.

Show »
Length:559
Mass (Da):61,659
Checksum:iDD26A38570FFB54E
GO

Sequence cautioni

The sequence BAC28426 differs from that shown. Reason: Frameshift at position 353.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → N in AAB92651 (PubMed:10022820).Curated1
Sequence conflicti164S → N in AAB92651 (PubMed:10022820).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0286261 – 35Missing in isoform 2. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_02862776 – 101Missing in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038029 mRNA Translation: AAB92651.1
AK033680 mRNA Translation: BAC28426.1 Frameshift.
BC055951 mRNA Translation: AAH55951.1
CCDSiCCDS22744.1 [O54972-1]
CCDS52696.1 [O54972-2]
RefSeqiNP_001103343.1, NM_001109873.1
NP_033954.2, NM_009824.2
NP_796263.1, NM_177289.1 [O54972-2]
UniGeneiMm.194339

Genome annotation databases

EnsembliENSMUST00000006525; ENSMUSP00000006525; ENSMUSG00000006362 [O54972-2]
GeneIDi12398
KEGGimmu:12398
UCSCiuc009ntl.2 mouse [O54972-1]
uc009ntm.2 mouse [O54972-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMTG16_MOUSE
AccessioniPrimary (citable) accession number: O54972
Secondary accession number(s): Q7TND6, Q8BZS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: March 28, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health