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Protein

Protein CBFA2T3

Gene

Cbfa2t3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri533 – 569MYND-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CBFA2T3
Alternative name(s):
Eight twenty one protein 2
MTG8-related protein 2
Protein ETO-2
Gene namesi
Name:Cbfa2t3
Synonyms:Cbfa2t3h, Mtgr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1338013. Cbfa2t3.

Subcellular locationi

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • intracellular Source: MGI
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071741 – 620Protein CBFA2T3Add BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei603PhosphoserineBy similarity1
Modified residuei607PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54972.
PaxDbiO54972.
PRIDEiO54972.

PTM databases

iPTMnetiO54972.
PhosphoSitePlusiO54972.

Expressioni

Tissue specificityi

Widely expressed with higher expression in heart, brain, lung and spleen. Expressed in hematopoietic cells (at protein level).2 Publications

Developmental stagei

Expressed during embryogenesis from 7 to 17 dpc.1 Publication

Inductioni

Up-regulated during hematopoietic differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000006362.
ExpressionAtlasiO54972. baseline and differential.
GenevisibleiO54972. MM.

Interactioni

Subunit structurei

Heterodimer with RUNX1T1 and CBFA2T2. Interacts with ZNF652 and ERBB4. May interact with PRKAR2A, PDE7A and probably PDE4A. Interacts with PLXNA1, PLXNA3 and PRKAR1A (By similarity). Interacts with HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, NCOR1 and NCOR2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with ZBTB4, ZBTB38 and ZBTB33. Interacts with HIF1A and EGLN1 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ldb1P706622EBI-8006703,EBI-6272082
Tal1P220913EBI-8006703,EBI-8006437
Tcf4Q607222EBI-8006703,EBI-310070

Protein-protein interaction databases

BioGridi198523. 2 interactors.
IntActiO54972. 5 interactors.
MINTiMINT-2568147.
STRINGi10090.ENSMUSP00000118997.

Structurei

3D structure databases

ProteinModelPortaliO54972.
SMRiO54972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 241TAFHPROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 412Mediates localization to the nucleusAdd BLAST412
Regioni120 – 217Interaction with ZBTB33By similarityAdd BLAST98
Regioni151 – 243Interaction with HIF1ABy similarityAdd BLAST93
Regioni371 – 389Nervy homology region 2 (NHR2); essential for down-regulation of PFKFB3, PFKFB4 and PDK1 expressionBy similarityAdd BLAST19
Regioni462 – 510Nervy homology region 3 (NHR3); essential for down-regulation of PFKFB3, PFKFB4 and PDK1 expressionBy similarityAdd BLAST49
Regioni462 – 483Mediates interaction with PRKAR2ABy similarityAdd BLAST22

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili465 – 520Sequence analysisAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi37 – 73Pro-richAdd BLAST37

Domaini

Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure.By similarity

Sequence similaritiesi

Belongs to the CBFA2T family.Curated
Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 TAFH (NHR1) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri533 – 569MYND-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IG1Z. Eukaryota.
ENOG410XR30. LUCA.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiO54972.

Family and domain databases

InterProiIPR013289. CBFA2T1/2/3.
IPR013292. CBFA2T3.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSiPR01875. ETOFAMILY.
PR01878. MTG16PROTEIN.
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEiPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54972-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQASTTTLE SGALLSGPRG LQYGSPAHRK EKAAAMPDSP AEVKTQPRST
60 70 80 90 100
PPSMPPPPPT SSQGATRPPS FTPHTHGEDG PATSLPHGRF HGCLKWSMVC
110 120 130 140 150
LLMNGSSHSP TAIHGAPSTP NGFSNGPATS STASLSTQHL PPACGARQLS
160 170 180 190 200
KLKRFLTTLQ QFGSDISPEI GERVRTLVLG LVNSTLTIEE FHAKLQEATN
210 220 230 240 250
FPLRPFVIPF LKANLPLLQR ELLHCARLAK QTPAQYLAQH EQLLLDASAT
260 270 280 290 300
SPVDSSELLL EVNENGKRRT PDRTKENGSD RDPLHPDHLS KRSCTLSPAQ
310 320 330 340 350
RCSPSNGLPH PTPPPPPHYR LEDMAMAHHF RDSYRHPDPR ELRERHRPLA
360 370 380 390 400
IPGSRQEEVI DHRLTEREWA EEWKHLNSLL NCIMDMVEKT RRSLTVLRRC
410 420 430 440 450
QEADREELNH WIRCYSDSEE GKKGPTPISA RSLNSCSGPE GSQLDVHRDF
460 470 480 490 500
TPRTLSGYMP EEIWRKAEEA VNEVKRQAMS ELQKAVSDAE RKAHELITTE
510 520 530 540 550
RAKMERALAE AKRQASEDAL TVINQQEDSS ESCWNCGRKA SETCSGCNAA
560 570 580 590 600
RYCGSFCQHK DWEKHHHVCG QSLQGPAAAV ADPLPGQPDA TASPSEAGSA
610 620
GPSRPCSPGP PGPLDAAVPR
Length:620
Mass (Da):68,032
Last modified:October 23, 2007 - v2
Checksum:i78DC1995BE365E43
GO
Isoform 2 (identifier: O54972-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     76-101: Missing.

Show »
Length:559
Mass (Da):61,659
Checksum:iDD26A38570FFB54E
GO

Sequence cautioni

The sequence BAC28426 differs from that shown. Reason: Frameshift at position 353.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → N in AAB92651 (PubMed:10022820).Curated1
Sequence conflicti164S → N in AAB92651 (PubMed:10022820).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0286261 – 35Missing in isoform 2. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_02862776 – 101Missing in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038029 mRNA. Translation: AAB92651.1.
AK033680 mRNA. Translation: BAC28426.1. Frameshift.
BC055951 mRNA. Translation: AAH55951.1.
CCDSiCCDS22744.1. [O54972-1]
CCDS52696.1. [O54972-2]
RefSeqiNP_001103343.1. NM_001109873.1.
NP_033954.2. NM_009824.2.
NP_796263.1. NM_177289.1. [O54972-2]
UniGeneiMm.194339.

Genome annotation databases

EnsembliENSMUST00000006525; ENSMUSP00000006525; ENSMUSG00000006362. [O54972-2]
GeneIDi12398.
KEGGimmu:12398.
UCSCiuc009ntl.2. mouse. [O54972-1]
uc009ntm.2. mouse. [O54972-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038029 mRNA. Translation: AAB92651.1.
AK033680 mRNA. Translation: BAC28426.1. Frameshift.
BC055951 mRNA. Translation: AAH55951.1.
CCDSiCCDS22744.1. [O54972-1]
CCDS52696.1. [O54972-2]
RefSeqiNP_001103343.1. NM_001109873.1.
NP_033954.2. NM_009824.2.
NP_796263.1. NM_177289.1. [O54972-2]
UniGeneiMm.194339.

3D structure databases

ProteinModelPortaliO54972.
SMRiO54972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198523. 2 interactors.
IntActiO54972. 5 interactors.
MINTiMINT-2568147.
STRINGi10090.ENSMUSP00000118997.

PTM databases

iPTMnetiO54972.
PhosphoSitePlusiO54972.

Proteomic databases

MaxQBiO54972.
PaxDbiO54972.
PRIDEiO54972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006525; ENSMUSP00000006525; ENSMUSG00000006362. [O54972-2]
GeneIDi12398.
KEGGimmu:12398.
UCSCiuc009ntl.2. mouse. [O54972-1]
uc009ntm.2. mouse. [O54972-2]

Organism-specific databases

CTDi863.
MGIiMGI:1338013. Cbfa2t3.

Phylogenomic databases

eggNOGiENOG410IG1Z. Eukaryota.
ENOG410XR30. LUCA.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiO54972.

Miscellaneous databases

ChiTaRSiCbfa2t3. mouse.
PROiO54972.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006362.
ExpressionAtlasiO54972. baseline and differential.
GenevisibleiO54972. MM.

Family and domain databases

InterProiIPR013289. CBFA2T1/2/3.
IPR013292. CBFA2T3.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSiPR01875. ETOFAMILY.
PR01878. MTG16PROTEIN.
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEiPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTG16_MOUSE
AccessioniPrimary (citable) accession number: O54972
Secondary accession number(s): Q7TND6, Q8BZS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.