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O54972 (MTG16_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CBFA2T3
Alternative name(s):
Eight twenty one protein 2
MTG8-related protein 2
Protein ETO-2
Gene names
Name:Cbfa2t3
Synonyms:Cbfa2t3h, Mtgr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length620 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation. May also function as an A-kinase-anchoring protein. Ref.4 Ref.5 Ref.6

Subunit structure

Heterodimer with RUNX1T1 and CBFA2T2. Interacts with ZNF652 and ERBB4. May interact with PRKAR2A, PDE7A and probably PDE4A. Interacts with PLXNA1, PLXNA3 and PRKAR1A By similarity. Interacts with HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, NCOR1 and NCOR2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Ref.4 Ref.5 Ref.6

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm. Golgi apparatus membrane By similarity Ref.1.

Tissue specificity

Widely expressed with higher expression in heart, brain, lung and spleen. Expressed in hematopoietic cells (at protein level). Ref.1 Ref.5

Developmental stage

Expressed during embryogenesis from 7 to 17 dpc. Ref.1

Induction

Up-regulated during hematopoietic differentiation. Ref.7

Sequence similarities

Belongs to the CBFA2T family.

Contains 1 MYND-type zinc finger.

Contains 1 TAFH (NHR1) domain.

Sequence caution

The sequence BAC28426.1 differs from that shown. Reason: Frameshift at position 353.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O54972-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O54972-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     76-101: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 620620Protein CBFA2T3
PRO_0000307174

Regions

Domain146 – 24196TAFH
Zinc finger533 – 56937MYND-type
Region1 – 412412Mediates localization to the nucleus
Region462 – 48322Mediates interaction with PRKAR2A By similarity
Coiled coil465 – 52056 Potential
Compositional bias37 – 7337Pro-rich

Amino acid modifications

Cross-link512Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence1 – 3535Missing in isoform 2.
VSP_028626
Alternative sequence76 – 10126Missing in isoform 2.
VSP_028627

Experimental info

Sequence conflict1501S → N in AAB92651. Ref.1
Sequence conflict1641S → N in AAB92651. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: 78DC1995BE365E43

FASTA62068,032
        10         20         30         40         50         60 
MSQASTTTLE SGALLSGPRG LQYGSPAHRK EKAAAMPDSP AEVKTQPRST PPSMPPPPPT 

        70         80         90        100        110        120 
SSQGATRPPS FTPHTHGEDG PATSLPHGRF HGCLKWSMVC LLMNGSSHSP TAIHGAPSTP 

       130        140        150        160        170        180 
NGFSNGPATS STASLSTQHL PPACGARQLS KLKRFLTTLQ QFGSDISPEI GERVRTLVLG 

       190        200        210        220        230        240 
LVNSTLTIEE FHAKLQEATN FPLRPFVIPF LKANLPLLQR ELLHCARLAK QTPAQYLAQH 

       250        260        270        280        290        300 
EQLLLDASAT SPVDSSELLL EVNENGKRRT PDRTKENGSD RDPLHPDHLS KRSCTLSPAQ 

       310        320        330        340        350        360 
RCSPSNGLPH PTPPPPPHYR LEDMAMAHHF RDSYRHPDPR ELRERHRPLA IPGSRQEEVI 

       370        380        390        400        410        420 
DHRLTEREWA EEWKHLNSLL NCIMDMVEKT RRSLTVLRRC QEADREELNH WIRCYSDSEE 

       430        440        450        460        470        480 
GKKGPTPISA RSLNSCSGPE GSQLDVHRDF TPRTLSGYMP EEIWRKAEEA VNEVKRQAMS 

       490        500        510        520        530        540 
ELQKAVSDAE RKAHELITTE RAKMERALAE AKRQASEDAL TVINQQEDSS ESCWNCGRKA 

       550        560        570        580        590        600 
SETCSGCNAA RYCGSFCQHK DWEKHHHVCG QSLQGPAAAV ADPLPGQPDA TASPSEAGSA 

       610        620 
GPSRPCSPGP PGPLDAAVPR 

« Hide

Isoform 2 [UniParc].

Checksum: DD26A38570FFB54E
Show »

FASTA55961,659

References

« Hide 'large scale' references
[1]"ETO-2, a new member of the ETO-family of nuclear proteins."
Davis J.N., Williams B.J., Herron J.T., Galiano F.J., Meyers S.
Oncogene 18:1375-1383(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Cecum.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 401-620 (ISOFORMS 1/2).
Strain: FVB/N.
Tissue: Colon.
[4]"ETO, a target of t(8;21) in acute leukemia, makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain."
Amann J.M., Nip J., Strom D.K., Lutterbach B., Harada H., Lenny N., Downing J.R., Meyers S., Hiebert S.W.
Mol. Cell. Biol. 21:6470-6483(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HDAC1; HDAC2; HDAC3; HDAC6; HDAC8; NCOR1 AND NCOR2.
[5]"AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation."
Ibanez V., Sharma A., Buonamici S., Verma A., Kalakonda S., Wang J., Kadkol S., Saunthararajah Y.
Cancer Res. 64:4547-4554(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NCOR1, TISSUE SPECIFICITY.
[6]"ETO2 coordinates cellular proliferation and differentiation during erythropoiesis."
Goardon N., Lambert J.A., Rodriguez P., Nissaire P., Herblot S., Thibault P., Dumenil D., Strouboulis J., Romeo P.-H., Hoang T.
EMBO J. 25:357-366(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN TAL-1 COMPLEX.
[7]"Expression of AML/Runx and ETO/MTG family members during hematopoietic differentiation of embryonic stem cells."
Okumura A.J., Peterson L.F., Lo M.-C., Zhang D.-E.
Exp. Hematol. 35:978-988(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF038029 mRNA. Translation: AAB92651.1.
AK033680 mRNA. Translation: BAC28426.1. Frameshift.
BC055951 mRNA. Translation: AAH55951.1.
CCDSCCDS22744.1. [O54972-1]
CCDS52696.1. [O54972-2]
RefSeqNP_001103343.1. NM_001109873.1.
NP_033954.2. NM_009824.2.
NP_796263.1. NM_177289.1. [O54972-2]
UniGeneMm.194339.

3D structure databases

ProteinModelPortalO54972.
SMRO54972. Positions 145-242, 360-419, 456-485, 528-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198523. 2 interactions.
IntActO54972. 5 interactions.
MINTMINT-2568147.

PTM databases

PhosphoSiteO54972.

Proteomic databases

PRIDEO54972.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006525; ENSMUSP00000006525; ENSMUSG00000006362. [O54972-2]
GeneID12398.
KEGGmmu:12398.
UCSCuc009ntl.2. mouse. [O54972-1]
uc009ntm.2. mouse. [O54972-2]

Organism-specific databases

CTD863.
MGIMGI:1338013. Cbfa2t3.

Phylogenomic databases

eggNOGNOG79810.
GeneTreeENSGT00390000013479.
HOGENOMHOG000049245.
HOVERGENHBG000169.
InParanoidO54972.

Gene expression databases

ArrayExpressO54972.
BgeeO54972.
GenevestigatorO54972.

Family and domain databases

InterProIPR013289. ETO.
IPR013292. MTG16.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PfamPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSPR01875. ETOFAMILY.
PR01878. MTG16PROTEIN.
SMARTSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCBFA2T3. mouse.
NextBio281154.
PROO54972.
SOURCESearch...

Entry information

Entry nameMTG16_MOUSE
AccessionPrimary (citable) accession number: O54972
Secondary accession number(s): Q7TND6, Q8BZS0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot