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O54972

- MTG16_MOUSE

UniProt

O54972 - MTG16_MOUSE

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Protein
Protein CBFA2T3
Gene
Cbfa2t3, Cbfa2t3h, Mtgr2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation. May also function as an A-kinase-anchoring protein.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri533 – 56937MYND-type
Add
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. sequence-specific DNA binding transcription factor activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CBFA2T3
Alternative name(s):
Eight twenty one protein 2
MTG8-related protein 2
Protein ETO-2
Gene namesi
Name:Cbfa2t3
Synonyms:Cbfa2t3h, Mtgr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1338013. Cbfa2t3.

Subcellular locationi

Nucleusnucleolus. Nucleusnucleoplasm. Golgi apparatus membrane By similarity 1 Publication

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. intracellular Source: MGI
  3. nucleolus Source: UniProtKB-SubCell
  4. nucleoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 620620Protein CBFA2T3
PRO_0000307174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki512 – 512Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiO54972.

PTM databases

PhosphoSiteiO54972.

Expressioni

Tissue specificityi

Widely expressed with higher expression in heart, brain, lung and spleen. Expressed in hematopoietic cells (at protein level).2 Publications

Developmental stagei

Expressed during embryogenesis from 7 to 17 dpc.1 Publication

Inductioni

Up-regulated during hematopoietic differentiation.1 Publication

Gene expression databases

ArrayExpressiO54972.
BgeeiO54972.
GenevestigatoriO54972.

Interactioni

Subunit structurei

Heterodimer with RUNX1T1 and CBFA2T2. Interacts with ZNF652 and ERBB4. May interact with PRKAR2A, PDE7A and probably PDE4A. Interacts with PLXNA1, PLXNA3 and PRKAR1A By similarity. Interacts with HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, NCOR1 and NCOR2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ldb1P706622EBI-8006703,EBI-6272082
Tal1P220913EBI-8006703,EBI-8006437
Tcf4Q607222EBI-8006703,EBI-310070

Protein-protein interaction databases

BioGridi198523. 2 interactions.
IntActiO54972. 5 interactions.
MINTiMINT-2568147.

Structurei

3D structure databases

ProteinModelPortaliO54972.
SMRiO54972. Positions 145-242, 360-419, 456-485, 528-575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini146 – 24196TAFH
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 412412Mediates localization to the nucleus
Add
BLAST
Regioni462 – 48322Mediates interaction with PRKAR2A By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili465 – 52056 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 7337Pro-rich
Add
BLAST

Sequence similaritiesi

Belongs to the CBFA2T family.
Contains 1 TAFH (NHR1) domain.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG79810.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiO54972.

Family and domain databases

InterProiIPR013289. ETO.
IPR013292. MTG16.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSiPR01875. ETOFAMILY.
PR01878. MTG16PROTEIN.
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEiPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O54972-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSQASTTTLE SGALLSGPRG LQYGSPAHRK EKAAAMPDSP AEVKTQPRST    50
PPSMPPPPPT SSQGATRPPS FTPHTHGEDG PATSLPHGRF HGCLKWSMVC 100
LLMNGSSHSP TAIHGAPSTP NGFSNGPATS STASLSTQHL PPACGARQLS 150
KLKRFLTTLQ QFGSDISPEI GERVRTLVLG LVNSTLTIEE FHAKLQEATN 200
FPLRPFVIPF LKANLPLLQR ELLHCARLAK QTPAQYLAQH EQLLLDASAT 250
SPVDSSELLL EVNENGKRRT PDRTKENGSD RDPLHPDHLS KRSCTLSPAQ 300
RCSPSNGLPH PTPPPPPHYR LEDMAMAHHF RDSYRHPDPR ELRERHRPLA 350
IPGSRQEEVI DHRLTEREWA EEWKHLNSLL NCIMDMVEKT RRSLTVLRRC 400
QEADREELNH WIRCYSDSEE GKKGPTPISA RSLNSCSGPE GSQLDVHRDF 450
TPRTLSGYMP EEIWRKAEEA VNEVKRQAMS ELQKAVSDAE RKAHELITTE 500
RAKMERALAE AKRQASEDAL TVINQQEDSS ESCWNCGRKA SETCSGCNAA 550
RYCGSFCQHK DWEKHHHVCG QSLQGPAAAV ADPLPGQPDA TASPSEAGSA 600
GPSRPCSPGP PGPLDAAVPR 620
Length:620
Mass (Da):68,032
Last modified:October 23, 2007 - v2
Checksum:i78DC1995BE365E43
GO
Isoform 2 (identifier: O54972-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     76-101: Missing.

Show »
Length:559
Mass (Da):61,659
Checksum:iDD26A38570FFB54E
GO

Sequence cautioni

The sequence BAC28426.1 differs from that shown. Reason: Frameshift at position 353.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3535Missing in isoform 2.
VSP_028626Add
BLAST
Alternative sequencei76 – 10126Missing in isoform 2.
VSP_028627Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501S → N in AAB92651. 1 Publication
Sequence conflicti164 – 1641S → N in AAB92651. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF038029 mRNA. Translation: AAB92651.1.
AK033680 mRNA. Translation: BAC28426.1. Frameshift.
BC055951 mRNA. Translation: AAH55951.1.
CCDSiCCDS22744.1. [O54972-1]
CCDS52696.1. [O54972-2]
RefSeqiNP_001103343.1. NM_001109873.1.
NP_033954.2. NM_009824.2.
NP_796263.1. NM_177289.1. [O54972-2]
UniGeneiMm.194339.

Genome annotation databases

EnsembliENSMUST00000006525; ENSMUSP00000006525; ENSMUSG00000006362. [O54972-2]
GeneIDi12398.
KEGGimmu:12398.
UCSCiuc009ntl.2. mouse. [O54972-1]
uc009ntm.2. mouse. [O54972-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF038029 mRNA. Translation: AAB92651.1 .
AK033680 mRNA. Translation: BAC28426.1 . Frameshift.
BC055951 mRNA. Translation: AAH55951.1 .
CCDSi CCDS22744.1. [O54972-1 ]
CCDS52696.1. [O54972-2 ]
RefSeqi NP_001103343.1. NM_001109873.1.
NP_033954.2. NM_009824.2.
NP_796263.1. NM_177289.1. [O54972-2 ]
UniGenei Mm.194339.

3D structure databases

ProteinModelPortali O54972.
SMRi O54972. Positions 145-242, 360-419, 456-485, 528-575.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198523. 2 interactions.
IntActi O54972. 5 interactions.
MINTi MINT-2568147.

PTM databases

PhosphoSitei O54972.

Proteomic databases

PRIDEi O54972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006525 ; ENSMUSP00000006525 ; ENSMUSG00000006362 . [O54972-2 ]
GeneIDi 12398.
KEGGi mmu:12398.
UCSCi uc009ntl.2. mouse. [O54972-1 ]
uc009ntm.2. mouse. [O54972-2 ]

Organism-specific databases

CTDi 863.
MGIi MGI:1338013. Cbfa2t3.

Phylogenomic databases

eggNOGi NOG79810.
GeneTreei ENSGT00390000013479.
HOGENOMi HOG000049245.
HOVERGENi HBG000169.
InParanoidi O54972.

Miscellaneous databases

ChiTaRSi CBFA2T3. mouse.
NextBioi 281154.
PROi O54972.
SOURCEi Search...

Gene expression databases

ArrayExpressi O54972.
Bgeei O54972.
Genevestigatori O54972.

Family and domain databases

InterProi IPR013289. ETO.
IPR013292. MTG16.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view ]
Pfami PF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view ]
PRINTSi PR01875. ETOFAMILY.
PR01878. MTG16PROTEIN.
SMARTi SM00549. TAFH. 1 hit.
[Graphical view ]
PROSITEi PS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ETO-2, a new member of the ETO-family of nuclear proteins."
    Davis J.N., Williams B.J., Herron J.T., Galiano F.J., Meyers S.
    Oncogene 18:1375-1383(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cecum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 401-620 (ISOFORMS 1/2).
    Strain: FVB/N.
    Tissue: Colon.
  4. "ETO, a target of t(8;21) in acute leukemia, makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain."
    Amann J.M., Nip J., Strom D.K., Lutterbach B., Harada H., Lenny N., Downing J.R., Meyers S., Hiebert S.W.
    Mol. Cell. Biol. 21:6470-6483(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC1; HDAC2; HDAC3; HDAC6; HDAC8; NCOR1 AND NCOR2.
  5. "AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation."
    Ibanez V., Sharma A., Buonamici S., Verma A., Kalakonda S., Wang J., Kadkol S., Saunthararajah Y.
    Cancer Res. 64:4547-4554(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NCOR1, TISSUE SPECIFICITY.
  6. "ETO2 coordinates cellular proliferation and differentiation during erythropoiesis."
    Goardon N., Lambert J.A., Rodriguez P., Nissaire P., Herblot S., Thibault P., Dumenil D., Strouboulis J., Romeo P.-H., Hoang T.
    EMBO J. 25:357-366(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN TAL-1 COMPLEX.
  7. "Expression of AML/Runx and ETO/MTG family members during hematopoietic differentiation of embryonic stem cells."
    Okumura A.J., Peterson L.F., Lo M.-C., Zhang D.-E.
    Exp. Hematol. 35:978-988(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiMTG16_MOUSE
AccessioniPrimary (citable) accession number: O54972
Secondary accession number(s): Q7TND6, Q8BZS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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