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Protein

RE1-silencing transcription factor

Gene

Rest

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (PubMed:9454838). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 180C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri215 – 237C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri247 – 269C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri275 – 297C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri303 – 325C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri331 – 354C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri360 – 382C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri388 – 411C2H2-type 8PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1032 – 1054C2H2-type 9PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • DNA binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • outward rectifier potassium channel activity Source: UniProtKB
  • protein complex binding Source: RGD
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RE1-silencing transcription factor
Alternative name(s):
Neural-restrictive silencer factor
Gene namesi
Name:Rest
Synonyms:Nrsf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621069. Rest.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with ZFP90 in the nucleus.By similarity

GO - Cellular componenti

  • chromatin Source: RGD
  • nucleus Source: UniProtKB
  • protein complex Source: RGD
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002695491 – 1069RE1-silencing transcription factorAdd BLAST1069

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei948PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54963.
PRIDEiO54963.

PTM databases

iPTMnetiO54963.
PhosphoSitePlusiO54963.

Expressioni

Tissue specificityi

Expressed in the brain, with the highest levels in the neurons of hippocampus, pons/medulla and midbrain.

Developmental stagei

Levels decrease during brain development.1 Publication

Inductioni

Up-regulated by kainic acid.1 Publication

Interactioni

Subunit structurei

Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. Interacts (via zinc-finger DNA-binding domain) with ZFP90 (via N- and C-termini); the interaction inhibits REST repressor activity.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi249783. 2 interactors.
STRINGi10116.ENSRNOP00000002837.

Structurei

3D structure databases

ProteinModelPortaliO54963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 121Interaction with SIN3ABy similarityAdd BLAST90
Regioni43 – 57Interaction with SIN3BBy similarityAdd BLAST15
Regioni981 – 1059Interaction with RCOR1By similarityAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi440 – 444Poly-Ala5
Compositional biasi520 – 605Lys-richAdd BLAST86
Compositional biasi663 – 753Pro-richAdd BLAST91

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 180C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri215 – 237C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri247 – 269C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri275 – 297C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri303 – 325C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri331 – 354C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri360 – 382C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri388 – 411C2H2-type 8PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1032 – 1054C2H2-type 9PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234631.
HOVERGENiHBG093893.
InParanoidiO54963.
KOiK09222.
PhylomeDBiO54963.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54963-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATQVMGQSS GGGSLFNNSG NMGMALPNDM YDLHDLSKAE LAAPQLIMLA
60 70 80 90 100
NVALTGEVNG SCCDYLVGEE RQMAELMPVG DNHFSDSEGE GLEESAELKG
110 120 130 140 150
DPSGLDNMEL RSLELSVVEP QPVFEASAAP EVYSSNKDPA PEAPVAEDKC
160 170 180 190 200
KNLKAKPFRC KPCQYEAESE EQFVHHIRVH SAKKFFVEES AEKQAKARES
210 220 230 240 250
GASPSEEGEF SKGPIRCDRC GYNTNRYDHY TAHLKHHLRA GDNERVYKCI
260 270 280 290 300
ICTYTTVSEY HWRKHLRNHF PRKVYTCSKC NYFSTEKNNY VQHVRTHTGE
310 320 330 340 350
RPYKCELCPY SSSQKTHLTR HMRTHSGEKP FKCDQCNYVA SNQHEVTRHA
360 370 380 390 400
RQVHNGPKPL NCPHCDYKTA DRSNFKKHVE LHVNPRQFNC PVCDYAASKK
410 420 430 440 450
CNLQYHFKSK HPTCPSKTMD VSKVKLKKTK RREADLHRDA AAAATEQTDT
460 470 480 490 500
EQAKTKGVDA SARRSERPVK GVGKDVPKEK KPCSNASVVQ VTTRTRKSAV
510 520 530 540 550
ETKAAEGKHT DGQTGNNAEK SSKAKKSKRK MDAEAHPSVE PVTEGPVTKK
560 570 580 590 600
KKTESKPKTS GEVPKGSRVE DRKADKQQSA SIKKGGKKTA LKTKTAKKGS
610 620 630 640 650
KLAPKWVGHT EPSSEMAQGG ESPVPALTQA VVTPSGSTQT ELSSPMDIAQ
660 670 680 690 700
TEPAQMDVSQ TGPPQVQRPL PVEPAQLEPS PPQEPPQVEP PACVEPPPPV
710 720 730 740 750
EPPCPMEPAE MEPSPPMEPS QVEPPPHLEP PLPMELPQVE LPPVEDCQKE
760 770 780 790 800
LPPVEHAQTK VAQTGPTQVG AVQEEPLFCL RATSSQANQK VISPKDRAKE
810 820 830 840 850
KLSVLSEMAR QEQVLIEVGL VPVRDSQLLK ASKSAPDLPA PPSPLPKGHL
860 870 880 890 900
RREETPKDQE MFSDGEGNKV SPLEKGGTEE AGESRAELAA PMESTSALSS
910 920 930 940 950
EQSSNAPDGE TLHSECQADS TAVCEMEVDT EQKTDRVPLK DSAVEPVSPL
960 970 980 990 1000
NPRVDPEAAA PAVVASPPIT LAESQEIDED EGIHSHDGSD LSDNMSEGSD
1010 1020 1030 1040 1050
DSGLHGARPA PQEATSKSGK EGLAVKVTEG EFVCIFCDRS FRKEKDYSKH
1060
LNRHLVNVYF LEEAAEEQE
Length:1,069
Mass (Da):117,126
Last modified:June 1, 1998 - v1
Checksum:i0A040248D8526992
GO
Isoform 2 (identifier: O54963-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     300-302: ERP → KRA
     303-1069: Missing.

Show »
Length:302
Mass (Da):33,808
Checksum:i8B2E58D2A3030930
GO
Isoform 3 (identifier: O54963-3) [UniParc]FASTAAdd to basket
Also known as: REST1

The sequence of this isoform differs from the canonical sequence as follows:
     300-1069: Missing.

Show »
Length:299
Mass (Da):33,452
Checksum:iA2A3030930AE9F9E
GO
Isoform 4 (identifier: O54963-4) [UniParc]FASTAAdd to basket
Also known as: REST2

The sequence of this isoform differs from the canonical sequence as follows:
     328-328: E → W
     329-1069: Missing.

Show »
Length:328
Mass (Da):36,954
Checksum:iF756C8D12253B085
GO
Isoform 5 (identifier: O54963-5) [UniParc]FASTAAdd to basket
Also known as: REST3

The sequence of this isoform differs from the canonical sequence as follows:
     328-329: EK → AI
     330-1069: Missing.

Show »
Length:329
Mass (Da):36,952
Checksum:iBFA9F6C8D12253B0
GO
Isoform 6 (identifier: O54963-6) [UniParc]FASTAAdd to basket
Also known as: REST4

The sequence of this isoform differs from the canonical sequence as follows:
     328-332: EKPFK → CDLVG
     333-1069: Missing.

Show »
Length:332
Mass (Da):37,256
Checksum:i5D77E2F55A96C8D1
GO
Isoform 7 (identifier: O54963-7) [UniParc]FASTAAdd to basket
Also known as: REST5

The sequence of this isoform differs from the canonical sequence as follows:
     328-336: EKPFKCDQC → CDLVGYVFR
     337-1069: Missing.

Show »
Length:336
Mass (Da):37,821
Checksum:i0D2FCBF5DD77E2F5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022073300 – 1069Missing in isoform 3. 1 PublicationAdd BLAST770
Alternative sequenceiVSP_022074300 – 302ERP → KRA in isoform 2. 1 Publication3
Alternative sequenceiVSP_022075303 – 1069Missing in isoform 2. 1 PublicationAdd BLAST767
Alternative sequenceiVSP_022076328 – 336EKPFKCDQC → CDLVGYVFR in isoform 7. 1 Publication9
Alternative sequenceiVSP_022077328 – 332EKPFK → CDLVG in isoform 6. 1 Publication5
Alternative sequenceiVSP_022078328 – 329EK → AI in isoform 5. 1 Publication2
Alternative sequenceiVSP_022079328E → W in isoform 4. 1 Publication1
Alternative sequenceiVSP_022080329 – 1069Missing in isoform 4. 1 PublicationAdd BLAST741
Alternative sequenceiVSP_022081330 – 1069Missing in isoform 5. 1 PublicationAdd BLAST740
Alternative sequenceiVSP_022082333 – 1069Missing in isoform 6. 1 PublicationAdd BLAST737
Alternative sequenceiVSP_022083337 – 1069Missing in isoform 7. 1 PublicationAdd BLAST733

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037199 mRNA. Translation: AAB94893.1.
AF037203 mRNA. Translation: AAB94894.1.
RefSeqiNP_113976.1. NM_031788.1. [O54963-1]
UniGeneiRn.10879.
Rn.200522.

Genome annotation databases

GeneIDi83618.
KEGGirno:83618.
UCSCiRGD:621069. rat. [O54963-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037199 mRNA. Translation: AAB94893.1.
AF037203 mRNA. Translation: AAB94894.1.
RefSeqiNP_113976.1. NM_031788.1. [O54963-1]
UniGeneiRn.10879.
Rn.200522.

3D structure databases

ProteinModelPortaliO54963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249783. 2 interactors.
STRINGi10116.ENSRNOP00000002837.

PTM databases

iPTMnetiO54963.
PhosphoSitePlusiO54963.

Proteomic databases

PaxDbiO54963.
PRIDEiO54963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83618.
KEGGirno:83618.
UCSCiRGD:621069. rat. [O54963-1]

Organism-specific databases

CTDi5978.
RGDi621069. Rest.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234631.
HOVERGENiHBG093893.
InParanoidiO54963.
KOiK09222.
PhylomeDBiO54963.

Miscellaneous databases

PROiO54963.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREST_RAT
AccessioniPrimary (citable) accession number: O54963
Secondary accession number(s): O54964
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.