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O54956 (DPOE2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase epsilon subunit 2

EC=2.7.7.7
Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B
Gene names
Name:Pole2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Participates in DNA repair and in chromosomal DNA replication.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.

Subcellular location

Nucleus.

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Sequence similarities

Belongs to the DNA polymerase epsilon subunit B family.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentepsilon DNA polymerase complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527DNA polymerase epsilon subunit 2
PRO_0000071563

Experimental info

Sequence conflict781M → V in AAH63772. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O54956 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 82940B0FD1584350

FASTA52759,387
        10         20         30         40         50         60 
MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID AVEKQPLSSN 

        70         80         90        100        110        120 
MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN SERKKFLPLS MTNHAAPTLF 

       130        140        150        160        170        180 
GTARDKAELY LERYTILHQR THRHELFTPP VIGSHLEESG SKFQLKTIET LLGSTSKVGD 

       190        200        210        220        230        240 
VIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF 

       250        260        270        280        290        300 
GFPPTEPSST TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV 

       310        320        330        340        350        360 
LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC EYPSIHQSSR 

       370        380        390        400        410        420 
FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT NPCRIQYCTQ EIIIFREDLV 

       430        440        450        460        470        480 
NKMCRNCVRF PSSSLDIPTH FVKTILSQGH LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI 

       490        500        510        520 
ADKYDPFTVT NTDCLCINPG SFPRSGFAFK VFYPSSKTVE DSKLQGF 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Jaw.
[3]"The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon."
Jokela M., Makiniemi M., Lehtonen S., Szpirer C., Hellman U., Syvaeoja J.E.
Nucleic Acids Res. 26:730-734(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-527.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK077592 mRNA. Translation: BAC36883.1.
BC063772 mRNA. Translation: AAH63772.1.
AF036898 mRNA. Translation: AAC40045.1.
RefSeqNP_035263.1. NM_011133.2.
UniGeneMm.9199.

3D structure databases

ProteinModelPortalO54956.
SMRO54956. Positions 1-75.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO54956.

Proteomic databases

PaxDbO54956.
PRIDEO54956.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974.
GeneID18974.
KEGGmmu:18974.
UCSCuc007nrv.1. mouse.

Organism-specific databases

CTD5427.
MGIMGI:1197514. Pole2.

Phylogenomic databases

eggNOGNOG311342.
GeneTreeENSGT00390000012435.
HOGENOMHOG000265254.
HOVERGENHBG061290.
InParanoidO54956.
KOK02325.
OMAILQQRTH.
OrthoDBEOG725DH7.
PhylomeDBO54956.
TreeFamTF103007.

Gene expression databases

BgeeO54956.
GenevestigatorO54956.

Family and domain databases

InterProIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]
PfamPF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]
PIRSFPIRSF000799. DNA_pol_eps_2. 1 hit.
ProtoNetSearch...

Other

NextBio295332.
PROO54956.
SOURCESearch...

Entry information

Entry nameDPOE2_MOUSE
AccessionPrimary (citable) accession number: O54956
Secondary accession number(s): Q6P3Y7, Q8BP72
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot