Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O54956

- DPOE2_MOUSE

UniProt

O54956 - DPOE2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA polymerase epsilon subunit 2

Gene

Pole2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Participates in DNA repair and in chromosomal DNA replication.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_209299. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_219381. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_224446. Telomere C-strand synthesis initiation.
REACT_238214. DNA replication initiation.
REACT_254983. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 2 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B
Gene namesi
Name:Pole2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1197514. Pole2.

Subcellular locationi

GO - Cellular componenti

  1. epsilon DNA polymerase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527DNA polymerase epsilon subunit 2PRO_0000071563Add
BLAST

Proteomic databases

MaxQBiO54956.
PaxDbiO54956.
PRIDEiO54956.

PTM databases

PhosphoSiteiO54956.

Expressioni

Gene expression databases

BgeeiO54956.
GenevestigatoriO54956.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.

Structurei

3D structure databases

ProteinModelPortaliO54956.
SMRiO54956. Positions 1-75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG311342.
GeneTreeiENSGT00390000012435.
HOGENOMiHOG000265254.
HOVERGENiHBG061290.
InParanoidiO54956.
KOiK02325.
OMAiILQQRTH.
OrthoDBiEOG725DH7.
PhylomeDBiO54956.
TreeFamiTF103007.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]
PIRSFiPIRSF000799. DNA_pol_eps_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O54956-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID
60 70 80 90 100
AVEKQPLSSN MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN
110 120 130 140 150
SERKKFLPLS MTNHAAPTLF GTARDKAELY LERYTILHQR THRHELFTPP
160 170 180 190 200
VIGSHLEESG SKFQLKTIET LLGSTSKVGD VIVLGMITQL KEGKFFLEDP
210 220 230 240 250
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF GFPPTEPSST
260 270 280 290 300
TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV
310 320 330 340 350
LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC
360 370 380 390 400
EYPSIHQSSR FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT
410 420 430 440 450
NPCRIQYCTQ EIIIFREDLV NKMCRNCVRF PSSSLDIPTH FVKTILSQGH
460 470 480 490 500
LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI ADKYDPFTVT NTDCLCINPG
510 520
SFPRSGFAFK VFYPSSKTVE DSKLQGF
Length:527
Mass (Da):59,387
Last modified:August 30, 2005 - v2
Checksum:i82940B0FD1584350
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781M → V in AAH63772. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077592 mRNA. Translation: BAC36883.1.
BC063772 mRNA. Translation: AAH63772.1.
AF036898 mRNA. Translation: AAC40045.1.
CCDSiCCDS36463.1.
RefSeqiNP_035263.1. NM_011133.2.
UniGeneiMm.9199.

Genome annotation databases

EnsembliENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974.
GeneIDi18974.
KEGGimmu:18974.
UCSCiuc007nrv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077592 mRNA. Translation: BAC36883.1 .
BC063772 mRNA. Translation: AAH63772.1 .
AF036898 mRNA. Translation: AAC40045.1 .
CCDSi CCDS36463.1.
RefSeqi NP_035263.1. NM_011133.2.
UniGenei Mm.9199.

3D structure databases

ProteinModelPortali O54956.
SMRi O54956. Positions 1-75.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O54956.

Proteomic databases

MaxQBi O54956.
PaxDbi O54956.
PRIDEi O54956.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021359 ; ENSMUSP00000021359 ; ENSMUSG00000020974 .
GeneIDi 18974.
KEGGi mmu:18974.
UCSCi uc007nrv.1. mouse.

Organism-specific databases

CTDi 5427.
MGIi MGI:1197514. Pole2.

Phylogenomic databases

eggNOGi NOG311342.
GeneTreei ENSGT00390000012435.
HOGENOMi HOG000265254.
HOVERGENi HBG061290.
InParanoidi O54956.
KOi K02325.
OMAi ILQQRTH.
OrthoDBi EOG725DH7.
PhylomeDBi O54956.
TreeFami TF103007.

Enzyme and pathway databases

Reactomei REACT_209299. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_219381. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_224446. Telomere C-strand synthesis initiation.
REACT_238214. DNA replication initiation.
REACT_254983. Activation of the pre-replicative complex.

Miscellaneous databases

NextBioi 295332.
PROi O54956.
SOURCEi Search...

Gene expression databases

Bgeei O54956.
Genevestigatori O54956.

Family and domain databases

InterProi IPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view ]
Pfami PF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000799. DNA_pol_eps_2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Jaw.
  3. "The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon."
    Jokela M., Makiniemi M., Lehtonen S., Szpirer C., Hellman U., Syvaeoja J.E.
    Nucleic Acids Res. 26:730-734(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-527.

Entry informationi

Entry nameiDPOE2_MOUSE
AccessioniPrimary (citable) accession number: O54956
Secondary accession number(s): Q6P3Y7, Q8BP72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 30, 2005
Last modified: November 26, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3