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Protein

DnaJ homolog subfamily B member 6

Gene

Dnajb6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • chaperone-mediated protein folding Source: MGI
  • chorio-allantoic fusion Source: MGI
  • chorion development Source: MGI
  • extracellular matrix organization Source: MGI
  • intermediate filament organization Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • negative regulation of inclusion body assembly Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • positive regulation of ATPase activity Source: MGI
  • protein folding Source: MGI
  • protein localization to nucleus Source: MGI
  • regulation of protein localization Source: MGI
  • syncytiotrophoblast cell differentiation involved in labyrinthine layer development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 6
Alternative name(s):
Heat shock protein J2
Short name:
HSJ-2
MRJ
mDj4
Gene namesi
Name:Dnajb6
Synonyms:Hsj2, Mrj
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1344381. Dnajb6.

Subcellular locationi

  • Cytoplasmperinuclear region By similarity
  • Nucleus By similarity
  • CytoplasmmyofibrilsarcomereZ line By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 365364DnaJ homolog subfamily B member 6PRO_0000071026Add
BLAST

Proteomic databases

EPDiO54946.
MaxQBiO54946.
PaxDbiO54946.
PRIDEiO54946.

PTM databases

iPTMnetiO54946.
PhosphoSiteiO54946.
SwissPalmiO54946.

Expressioni

Gene expression databases

BgeeiO54946.
CleanExiMM_DNAJB6.
ExpressionAtlasiO54946. baseline and differential.
GenevisibleiO54946. MM.

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with BAG3, HSPB8 and STUB1 (By similarity). Interacts with HSP70, KRT18 and PTTG. Isoform B interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation (By similarity). Interacts with ALKBH1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Arrb1Q8BWG86EBI-642500,EBI-641778

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204812. 6 interactions.
IntActiO54946. 4 interactions.
MINTiMINT-1713010.
STRINGi10090.ENSMUSP00000008733.

Structurei

3D structure databases

ProteinModelPortaliO54946.
SMRiO54946. Positions 1-106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 6967JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 147146Interaction with HSP70By similarityAdd
BLAST
Regioni120 – 235116Interaction with KRT18By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 817Poly-Gly
Compositional biasi83 – 17290Gly/Phe-richAdd
BLAST
Compositional biasi84 – 16582Phe-richAdd
BLAST
Compositional biasi158 – 19437Ser-richAdd
BLAST
Compositional biasi249 – 30759Pro-richAdd
BLAST
Compositional biasi356 – 3594Poly-Lys

Domaini

The antiaggregation activity of resides in the serine-rich region and the C-terminus.By similarity

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOVERGENiHBG066998.
InParanoidiO54946.
KOiK09512.
OMAiFFMPTSG.
OrthoDBiEOG7KSX9F.
PhylomeDBiO54946.
TreeFamiTF105142.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O54946-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVDYYEVLGV QRHASPEDIK KAYRKQALKW HPDKNPENKE EAERKFKQVA
60 70 80 90 100
EAYEVLSDAK KRDIYDKYGK EGLNGGGGGG GIHFDSPFEF GFTFRNPDDV
110 120 130 140 150
FREFFGGRDP FSFDFFEDPF DDFFGNRRGP RGNRSRGAGS FFSTFSGFPS
160 170 180 190 200
FGSGFPAFDT GFTPFGSLGH GGLTSFSSTS FGGSGMGNFK SISTSTKIVN
210 220 230 240 250
GKKITTKRIV ENGQERVEVE EDGQLKSLTI NGVADENALA EECQRRGQPT
260 270 280 290 300
PALAPGPAPA PVRVPSQARP LAPTPAPTPA PTPAPAPAQT PAPSVSTRPQ
310 320 330 340 350
KPPRPAPTAK LGSKSNWEDD EQDRQRVPGN WDAPMTSAGL KEGGKRKKQK
360
QKEDLKKKKS TKGNH
Note: No experimental confirmation available.
Length:365
Mass (Da):39,807
Last modified:June 26, 2007 - v4
Checksum:i7B0102218E8DAA99
GO
Isoform B (identifier: O54946-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-242: VADENALAEE → KEHLLRLDNK
     243-365: Missing.

Show »
Length:242
Mass (Da):26,978
Checksum:i3B56053C3BB0735D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171Missing in BAE41354 (PubMed:16141072).Curated
Sequence conflicti139 – 1402GS → AP in AAC16759 (Ref. 2) Curated
Sequence conflicti188 – 1892Missing in BAE25752 (PubMed:16141072).Curated
Sequence conflicti218 – 2181E → V in AAH03702 (PubMed:15489334).Curated
Sequence conflicti227 – 2271S → P in AAC16759 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei233 – 24210VADENALAEE → KEHLLRLDNK in isoform B. 4 PublicationsVSP_026212
Alternative sequencei243 – 365123Missing in isoform B. 4 PublicationsVSP_026213Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028854 mRNA. Translation: BAA88302.1.
AF035962 mRNA. Translation: AAC16759.1.
AK133805 mRNA. Translation: BAE21853.1.
AK134387 mRNA. Translation: BAE22124.1.
AK144182 mRNA. Translation: BAE25752.1.
AK151219 mRNA. Translation: BAE30213.1.
AK151752 mRNA. Translation: BAE30661.1.
AK152626 mRNA. Translation: BAE31369.1.
AK152632 mRNA. Translation: BAE31375.1.
AK153091 mRNA. Translation: BAE31714.1.
AK169332 mRNA. Translation: BAE41085.1.
AK169767 mRNA. Translation: BAE41354.1.
BC003702 mRNA. Translation: AAH03702.1.
BC083349 mRNA. Translation: AAH83349.1.
CCDSiCCDS19151.1. [O54946-1]
CCDS39043.1. [O54946-2]
RefSeqiNP_001033029.1. NM_001037940.4. [O54946-1]
NP_001120839.1. NM_001127367.1.
NP_035977.2. NM_011847.4. [O54946-2]
UniGeneiMm.290110.

Genome annotation databases

EnsembliENSMUST00000008733; ENSMUSP00000008733; ENSMUSG00000029131. [O54946-1]
ENSMUST00000114839; ENSMUSP00000110488; ENSMUSG00000029131. [O54946-2]
GeneIDi23950.
KEGGimmu:23950.
UCSCiuc008wup.2. mouse. [O54946-2]
uc008wur.2. mouse. [O54946-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028854 mRNA. Translation: BAA88302.1.
AF035962 mRNA. Translation: AAC16759.1.
AK133805 mRNA. Translation: BAE21853.1.
AK134387 mRNA. Translation: BAE22124.1.
AK144182 mRNA. Translation: BAE25752.1.
AK151219 mRNA. Translation: BAE30213.1.
AK151752 mRNA. Translation: BAE30661.1.
AK152626 mRNA. Translation: BAE31369.1.
AK152632 mRNA. Translation: BAE31375.1.
AK153091 mRNA. Translation: BAE31714.1.
AK169332 mRNA. Translation: BAE41085.1.
AK169767 mRNA. Translation: BAE41354.1.
BC003702 mRNA. Translation: AAH03702.1.
BC083349 mRNA. Translation: AAH83349.1.
CCDSiCCDS19151.1. [O54946-1]
CCDS39043.1. [O54946-2]
RefSeqiNP_001033029.1. NM_001037940.4. [O54946-1]
NP_001120839.1. NM_001127367.1.
NP_035977.2. NM_011847.4. [O54946-2]
UniGeneiMm.290110.

3D structure databases

ProteinModelPortaliO54946.
SMRiO54946. Positions 1-106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204812. 6 interactions.
IntActiO54946. 4 interactions.
MINTiMINT-1713010.
STRINGi10090.ENSMUSP00000008733.

PTM databases

iPTMnetiO54946.
PhosphoSiteiO54946.
SwissPalmiO54946.

Proteomic databases

EPDiO54946.
MaxQBiO54946.
PaxDbiO54946.
PRIDEiO54946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008733; ENSMUSP00000008733; ENSMUSG00000029131. [O54946-1]
ENSMUST00000114839; ENSMUSP00000110488; ENSMUSG00000029131. [O54946-2]
GeneIDi23950.
KEGGimmu:23950.
UCSCiuc008wup.2. mouse. [O54946-2]
uc008wur.2. mouse. [O54946-1]

Organism-specific databases

CTDi10049.
MGIiMGI:1344381. Dnajb6.

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOVERGENiHBG066998.
InParanoidiO54946.
KOiK09512.
OMAiFFMPTSG.
OrthoDBiEOG7KSX9F.
PhylomeDBiO54946.
TreeFamiTF105142.

Miscellaneous databases

ChiTaRSiDnajb6. mouse.
NextBioi303769.
PROiO54946.
SOURCEiSearch...

Gene expression databases

BgeeiO54946.
CleanExiMM_DNAJB6.
ExpressionAtlasiO54946. baseline and differential.
GenevisibleiO54946. MM.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature."
    Ohtsuka K., Hata M.
    Cell Stress Chaperones 5:98-112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. Hunter P.J., Swanson B.J., Haendel M., Lyons G.E., Cross J.C.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: 129.
    Tissue: Trophoblast.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
    Strain: BALB/cJ, C57BL/6J and NOD.
    Tissue: B-cell, Bone marrow, Embryo, Embryonic stomach, Embryonic testis and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: C57BL/6J and FVB/N.
    Tissue: Eye and Mammary gland.
  5. "Impaired placental trophoblast lineage differentiation in Alkbh1(-/-) mice."
    Pan Z., Sikandar S., Witherspoon M., Dizon D., Nguyen T., Benirschke K., Wiley C., Vrana P., Lipkin S.M.
    Dev. Dyn. 237:316-327(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALKBH1.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiDNJB6_MOUSE
AccessioniPrimary (citable) accession number: O54946
Secondary accession number(s): Q3TE94
, Q3U6L0, Q3UNJ5, Q3UYT7, Q99LA5, Q9QYI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 26, 2007
Last modified: March 16, 2016
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.