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Protein

Claudin-5

Gene

Cldn5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-5
Alternative name(s):
Brain endothelial cell clone 1 protein
Lung-specific membrane protein
Gene namesi
Name:Cldn5
Synonyms:Bec1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1276112. Cldn5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 12321CytoplasmicSequence analysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence analysisAdd
BLAST
Topological domaini145 – 16016ExtracellularSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Topological domaini182 – 21837CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Claudin-5PRO_0000144746Add
BLAST

Proteomic databases

MaxQBiO54942.
PaxDbiO54942.
PRIDEiO54942.

PTM databases

iPTMnetiO54942.
PhosphoSiteiO54942.

Expressioni

Tissue specificityi

Widely expressed with highest levels in the lung.1 Publication

Gene expression databases

BgeeiO54942.
CleanExiMM_CLDN5.
GenevisibleiO54942. MM.

Interactioni

Subunit structurei

Interacts with MPDZ (By similarity). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-113259.
STRINGi10090.ENSMUSP00000041925.

Structurei

3D structure databases

ProteinModelPortaliO54942.
SMRiO54942. Positions 4-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni217 – 2182Interactions with TJP1, TJP2 and TJP3By similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJT1. Eukaryota.
ENOG410Y9S8. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO54942.
KOiK06087.
OMAiLVCCGAW.
OrthoDBiEOG76HQ2T.
PhylomeDBiO54942.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003551. Claudin5.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01380. CLAUDIN5.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSAALEILG LVLCLVGWVG LILACGLPMW QVTAFLDHNI VTAQTTWKGL
60 70 80 90 100
WMSCVVQSTG HMQCKVYESV LALSAEVQAA RALTVGAVLL ALVALFVTLT
110 120 130 140 150
GAQCTTCVAP GPVKARVALT GGALYAVCGL LALVPLCWFA NIVVREFYDP
160 170 180 190 200
TVPVSQKYEL GAALYIGWAA SALLMCGGGL VCCGAWVCTG RPEFSFPVKY
210
SAPRRPTANG DYDKKNYV
Length:218
Mass (Da):23,054
Last modified:May 30, 2000 - v2
Checksum:i8708F69AE282DE87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581S → N in AAB96653 (PubMed:9520948).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035814 mRNA. Translation: AAB96653.1.
U82758 mRNA. Translation: AAC27545.1.
AF087823 mRNA. Translation: AAD09758.1.
AK077282 mRNA. Translation: BAC36728.1.
BC002016 mRNA. Translation: AAH02016.1.
BC083341 mRNA. Translation: AAH83341.1.
CCDSiCCDS28026.1.
RefSeqiNP_038833.2. NM_013805.4.
UniGeneiMm.22768.

Genome annotation databases

EnsembliENSMUST00000043577; ENSMUSP00000041925; ENSMUSG00000041378.
GeneIDi12741.
KEGGimmu:12741.
UCSCiuc007yok.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035814 mRNA. Translation: AAB96653.1.
U82758 mRNA. Translation: AAC27545.1.
AF087823 mRNA. Translation: AAD09758.1.
AK077282 mRNA. Translation: BAC36728.1.
BC002016 mRNA. Translation: AAH02016.1.
BC083341 mRNA. Translation: AAH83341.1.
CCDSiCCDS28026.1.
RefSeqiNP_038833.2. NM_013805.4.
UniGeneiMm.22768.

3D structure databases

ProteinModelPortaliO54942.
SMRiO54942. Positions 4-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-113259.
STRINGi10090.ENSMUSP00000041925.

PTM databases

iPTMnetiO54942.
PhosphoSiteiO54942.

Proteomic databases

MaxQBiO54942.
PaxDbiO54942.
PRIDEiO54942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043577; ENSMUSP00000041925; ENSMUSG00000041378.
GeneIDi12741.
KEGGimmu:12741.
UCSCiuc007yok.2. mouse.

Organism-specific databases

CTDi7122.
MGIiMGI:1276112. Cldn5.

Phylogenomic databases

eggNOGiENOG410IJT1. Eukaryota.
ENOG410Y9S8. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO54942.
KOiK06087.
OMAiLVCCGAW.
OrthoDBiEOG76HQ2T.
PhylomeDBiO54942.
TreeFamiTF331936.

Miscellaneous databases

NextBioi282058.
PROiO54942.
SOURCEiSearch...

Gene expression databases

BgeeiO54942.
CleanExiMM_CLDN5.
GenevisibleiO54942. MM.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003551. Claudin5.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01380. CLAUDIN5.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Brain capillary endothelial cells express MBEC1, a protein that is related to the Clostridium perfringens enterotoxin receptors."
    Chen Z., Zandonatti M., Jakubowski D., Fox H.S.
    Lab. Invest. 78:353-363(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "A mouse lung-specific membrane protein."
    Kim K.K., Baek D.H., Kwon B.S.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands."
    Morita K., Furuse M., Fujimoto K., Tsukita S.
    Proc. Natl. Acad. Sci. U.S.A. 96:511-516(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Ovary and Uterus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Mammary tumor.
  6. "Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins."
    Itoh M., Furuse M., Morita K., Kubota K., Saitou M., Tsukita S.
    J. Cell Biol. 147:1351-1363(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TJP1; TJP2 AND TJP3.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiCLD5_MOUSE
AccessioniPrimary (citable) accession number: O54942
Secondary accession number(s): O88789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 11, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.