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O54941

- SMCE1_MOUSE

UniProt

O54941 - SMCE1_MOUSE

Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

Gene

Smarce1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene By similarity. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.By similarity3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi66 – 13469HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. N-acetyltransferase activity Source: Ensembl
    3. protein binding Source: MGI
    4. RNA binding Source: MGI

    GO - Biological processi

    1. chromatin remodeling Source: Ensembl
    2. negative regulation of transcription, DNA-templated Source: Ensembl
    3. nervous system development Source: UniProtKB-KW
    4. nucleosome disassembly Source: Ensembl

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    Neurogenesis

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
    Alternative name(s):
    BRG1-associated factor 57
    Short name:
    BAF57
    Gene namesi
    Name:Smarce1
    Synonyms:Baf57
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1927347. Smarce1.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nBAF complex Source: UniProtKB
    2. npBAF complex Source: UniProtKB
    3. SWI/SNF complex Source: MGI
    4. transcriptional repressor complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1PRO_0000048578Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei265 – 2651PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Ubiquitination is prevented upon interaction between TRIP12 and SMARCC1 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO54941.
    PaxDbiO54941.
    PRIDEiO54941.

    PTM databases

    PhosphoSiteiO54941.

    Expressioni

    Developmental stagei

    Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5.1 Publication

    Gene expression databases

    ArrayExpressiO54941.
    BgeeiO54941.
    GenevestigatoriO54941.

    Interactioni

    Subunit structurei

    Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B), and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1 (SNF5/INI1). Other subunits specific to each of the complexes may also be present. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Also binds to the SRC/p160 family of histone acetyltransferases (HATs) composed of NCOA1, NCOA2, and NCOA3. NCOA3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with RCOR1/CoREST, NR3C1 and ZMIM2/ZIMP7. Interacts with BRDT By similarity. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi208275. 19 interactions.
    DIPiDIP-39985N.
    IntActiO54941. 9 interactions.
    MINTiMINT-1676035.

    Structurei

    3D structure databases

    ProteinModelPortaliO54941.
    SMRiO54941. Positions 66-139.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili222 – 31998Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi320 – 41192Glu-richAdd
    BLAST

    Domaini

    The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.

    Sequence similaritiesi

    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG291422.
    GeneTreeiENSGT00390000003628.
    HOGENOMiHOG000230965.
    HOVERGENiHBG054558.
    InParanoidiO54941.
    KOiK11651.
    OMAiSSMVPEE.
    OrthoDBiEOG7NKKKX.
    PhylomeDBiO54941.
    TreeFamiTF321146.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54941-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKRPSYAPP PTPAPATQMP STPGFVGYNP YSHLAYNNYR LGGNPGTNSR    50
    VTASSGITIP KPPKPPDKPL MPYMRYSRKV WDQVKASNPD LKLWEIGKII 100
    GGMWRDLTDE EKQEYLNEYE AEKIEYNESM KAYHNSPAYL AYINAKSRAE 150
    AALEEESRQR QSRMEKGEPY MSIQPAEDPD DYDDGFSMKH TATARFQRNH 200
    RLISEILSES VVPDVRSVVT TARMQVLKRQ VQSLMVHQRK LEAELLQIEE 250
    RHQEKKRKFL ESTDSFNNEL KRLCGLKVEV DMEKIAAEIA QAEEQARKRQ 300
    EEREKEAAEQ AERSQSSMAP EEEQVANKAE EKKDEESIPM ETEETHLEDT 350
    AESQQNGEEG TSTPEDKESG QEGVDSMEVE GTSDSNTGSE SNSATVEEPP 400
    TDPVPEDEKK E 411
    Length:411
    Mass (Da):46,638
    Last modified:June 1, 1998 - v1
    Checksum:i4099B333A0B63709
    GO

    Sequence cautioni

    The sequence BAC36233.1 differs from that shown. Reason: Sequencing errors.
    The sequence BAC36233.1 differs from that shown. Reason: Frameshift at positions 184, 212, 255, 316 and 353.
    The sequence BAC36233.1 differs from that shown. Reason: Erroneous termination at position 408. Translated as Glu.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035263 Genomic DNA. Translation: AAC04510.1.
    AK076177 mRNA. Translation: BAC36233.1. Sequence problems.
    BC047141 mRNA. Translation: AAH47141.1.
    BC061498 mRNA. Translation: AAH61498.1.
    BC065043 mRNA. Translation: AAH65043.1.
    CCDSiCCDS25374.1.
    RefSeqiNP_065643.1. NM_020618.4.
    UniGeneiMm.379086.
    Mm.389717.

    Genome annotation databases

    EnsembliENSMUST00000103133; ENSMUSP00000099422; ENSMUSG00000037935.
    GeneIDi57376.
    KEGGimmu:57376.
    UCSCiuc007lii.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035263 Genomic DNA. Translation: AAC04510.1 .
    AK076177 mRNA. Translation: BAC36233.1 . Sequence problems.
    BC047141 mRNA. Translation: AAH47141.1 .
    BC061498 mRNA. Translation: AAH61498.1 .
    BC065043 mRNA. Translation: AAH65043.1 .
    CCDSi CCDS25374.1.
    RefSeqi NP_065643.1. NM_020618.4.
    UniGenei Mm.379086.
    Mm.389717.

    3D structure databases

    ProteinModelPortali O54941.
    SMRi O54941. Positions 66-139.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208275. 19 interactions.
    DIPi DIP-39985N.
    IntActi O54941. 9 interactions.
    MINTi MINT-1676035.

    PTM databases

    PhosphoSitei O54941.

    Proteomic databases

    MaxQBi O54941.
    PaxDbi O54941.
    PRIDEi O54941.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103133 ; ENSMUSP00000099422 ; ENSMUSG00000037935 .
    GeneIDi 57376.
    KEGGi mmu:57376.
    UCSCi uc007lii.1. mouse.

    Organism-specific databases

    CTDi 6605.
    MGIi MGI:1927347. Smarce1.

    Phylogenomic databases

    eggNOGi NOG291422.
    GeneTreei ENSGT00390000003628.
    HOGENOMi HOG000230965.
    HOVERGENi HBG054558.
    InParanoidi O54941.
    KOi K11651.
    OMAi SSMVPEE.
    OrthoDBi EOG7NKKKX.
    PhylomeDBi O54941.
    TreeFami TF321146.

    Miscellaneous databases

    ChiTaRSi SMARCE1. mouse.
    NextBioi 313752.
    PROi O54941.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54941.
    Bgeei O54941.
    Genevestigatori O54941.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes."
      Wang W., Chi T., Xue Y., Zhou S., Kuo A., Crabtree G.R.
      Proc. Natl. Acad. Sci. U.S.A. 95:492-498(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain, Colon and Mammary tumor.
    4. "Targeting of SWI/SNF chromatin remodelling complexes to estrogen-responsive genes."
      Belandia B., Orford R.L., Hurst H.C., Parker M.G.
      EMBO J. 21:4094-4103(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes."
      Chi T.H., Wan M., Zhao K., Taniuchi I., Chen L., Littman D.R., Crabtree G.R.
      Nature 418:195-199(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "An essential switch in subunit composition of a chromatin remodeling complex during neural development."
      Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
      Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NBAF AND NPBAF COMPLEXES, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiSMCE1_MOUSE
    AccessioniPrimary (citable) accession number: O54941
    Secondary accession number(s): Q8BPD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3