Reviewed, UniProtKB/Swiss-Prot O54939 (DHB3_RAT)

Names and origin

Protein names

Recommended name:
    Testosterone 17-beta-dehydrogenase 3
    EC=1.1.1.64
Alternative name(s):
    Testicular 17-beta-hydroxysteroid dehydrogenase
    17-beta-HSD 3

Gene names

Name:	Hsd17b3
Synonyms:	Edh17b3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH By similarity.
Catalytic activity	Testosterone + NADP+ = androst-4-ene-3,17-dione + NADPH.
Pathway	Hormone biosynthesis; testosterone biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Traceable author statement. Source: RGD
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from direct assay. Source: RGD testosterone 17-beta-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Testosterone 17-beta-dehydrogenase 3		PRO_0000054575
Regions
	Nucleotide binding	44 – 73	30	NADP By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	181	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54939-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8B88584047614B53	FASTA	306	34,223
	10 20 30 40 50 60 MEQFLLSVGL LVCLVCLVKC VRFSRYLFLS FCKALPGSFL RSMGQWAVIT GAGDGIGKAY 70 80 90 100 110 120 SFELARHGLN VVLISRTLEK LQVISEEIER TTGSRVKVVQ ADFTREDIYD HIEEQLKGLE 130 140 150 160 170 180 IGVLVNNVGM LPNLLPSHFL STSGESQSVI HCNITSVVKM TQLVLKHMES RRRGLILNIS 190 200 210 220 230 240 SGVGVRPWPL YSLYSASKAF VCTFSKALNV EYRDKGIIIQ VLTPYSVSTP MTKYLNTSRV 250 260 270 280 290 300 TKTADEFVKE SLKYVTIGAE TCGCLAHEIL AIILNLIPSR IFYSSTTQRF LLKQFSDYLK SNISNR

References

[1]	Khanum A., Tsai-Morris C.-H., Dufau M.L. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF035156 mRNA. Translation: AAB99739.1.
IPI	IPI00199901.
RefSeq	NP_446459.1.
UniGene	Rn.10895
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000019096. Rattus norvegicus. [Contig view]
GeneID	117182.
KEGG	rno:117182.
Organism-specific databases
RGD	621805. Hsd17b3.
Phylogenomic databases
HOVERGEN	O54939.
Enzyme and pathway databases
BRENDA	1.1.1.64. 248.
Gene expression databases
ArrayExpress	O54939.
GermOnline	ENSRNOG00000019096. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620038.

Entry information

Entry name

DHB3_RAT

Accession

Primary (citable) accession number: O54939

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)