Reviewed, UniProtKB/Swiss-Prot O54937 (PDK4_RAT)

Names and origin

Protein names

Recommended name:
    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
    EC=2.7.11.2
Alternative name(s):
    Pyruvate dehydrogenase kinase isoform 4

Gene names

Name:

Pdk4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Ubiquitous; highest levels of expression in heart and skeletal muscle.
Induction	Expression increases several fold during starvation.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	acetyl-CoA biosynthetic process from pyruvate Traceable author statement. Source: RGD glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: RGD pyruvate dehydrogenase (acetyl-transferring) kinase activity Ref.1 Inferred from direct assay. Source: RGD two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 412		[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial		PRO_0000023447
Regions
	Domain	138 – 368	231	Histidine kinase
Amino acid modifications
	Modified residue	127	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54937-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 964A5DAAB189427A	FASTA	412	46,679
	10 20 30 40 50 60 MKAARFVMRS ASSLGNAGLV PREVELFSRY SPSPLSMKQL LDFGSENACE RTSFSFLRQE 70 80 90 100 110 120 LPVRLANILK EIDILPEHLV NTPSVQLVKS WYIQSLMDLV EFHEKSPEDQ KVLSDFVDTL 130 140 150 160 170 180 VKVRNRHHNV VPTMAQGILE YKDNCTVDPV TNQNLQYFLD RFYMNRISTR MLMNQHILIF 190 200 210 220 230 240 SDSKTGNPSH IGSIDPNCDV VAVVEDAFEC AKMLCDQYYL TSPELKLTQV NGKFPGQPIH 250 260 270 280 290 300 IVYVPSHLHH MLFELFKNAM RATVEHQENR PFLTPVEATV VLGKEDLTIK ISDRGGGVPL 310 320 330 340 350 360 RITDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL YSMSGYGTDA 370 380 390 400 410 IIYLKALSSE SIEKLPVFNK SAFKHYQMSS EADDWCIPSK EPKNLSKEKL AV

References

[1]	"Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex." Bowker-Kinley M.M., Davis W.I., Wu P., Harris R.A., Popov K.M. Biochem. J. 329:191-196(1998) [PubMed: 9405293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF034577 mRNA. Translation: AAC00177.1.
IPI	IPI00199898.
RefSeq	NP_446003.1.
UniGene	Rn.30070
3D structure databases
HSSP	HSSP built from PDB template 1JM6 based on UniProtKB Q64536.
ModBase	Search...
Protein-protein interaction databases
STRING	O54937.
Proteomic databases
PRIDE	O54937.
Genome annotation databases
Ensembl	ENSRNOT00000012760; ENSRNOP00000012759; ENSRNOG00000009565; Rattus norvegicus. [Genome view]
GeneID	89813.
KEGG	rno:89813.
UCSC	NM_053551. rat.
Organism-specific databases
CTD	89813.
RGD	69061. Pdk4.
Phylogenomic databases
HOVERGEN	O54937.
Enzyme and pathway databases
BRENDA	2.7.11.2. 248.
Gene expression databases
ArrayExpress	O54937.
Genevestigator	O54937.
GermOnline	ENSRNOG00000009565. Rattus norvegicus.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR018955. BCDHK/PDK_mit. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617690.

Entry information

Entry name

PDK4_RAT

Accession

Primary (citable) accession number: O54937

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	June 1, 1998
Last modified:	October 13, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)