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Protein

Apoptosis regulatory protein Siva

Gene

Siva1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis (By similarity).By similarity1 Publication

Cofactori

Zn2+By similarityNote: Isoform 1 binds 3 zinc ions. Isoform 2 binds 2 zinc ions.By similarity

GO - Molecular functioni

  • CD27 receptor binding Source: HGNC
  • tumor necrosis factor receptor binding Source: HGNC
  • virus receptor activity Source: HGNC
  • zinc ion binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulatory protein Siva
Alternative name(s):
CD27-binding protein
Short name:
CD27BP
Gene namesi
Name:Siva1
Synonyms:Siva
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1353606. Siva1.

Subcellular locationi

  • Cytoplasm
  • Nucleus By similarity

  • Note: In the nucleus, accumulates in dot-like structures.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Apoptosis regulatory protein SivaPRO_0000097775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Phosphotyrosine; by ABL2By similarity
Modified residuei70 – 701PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by ABL2/ARG in response to oxidative stress.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO54926.
MaxQBiO54926.
PaxDbiO54926.
PeptideAtlasiO54926.
PRIDEiO54926.

PTM databases

PhosphoSiteiO54926.

Expressioni

Tissue specificityi

Highly expressed in testis, heart, liver, lung, and muscle, and less in kidney, spleen and brain.

Inductioni

By p53, camptothecin, stroke injury and infection with coxsackievirus B3. This up-regulation is sufficient to induce apoptosis in neural tissue.2 Publications

Gene expression databases

BgeeiO54926.
CleanExiMM_SIVA1.
GenevisibleiO54926. MM.

Interactioni

Subunit structurei

Binds through its N-terminal region to the C-terminus of CD27 and to PXMP2/PMP22. Binds to the C-terminus of TNFRSF18/GITR. Isoform 1 binds to BCL2L1/BCLX isoform Bcl-x(L) but not to BAX.1 Publication

GO - Molecular functioni

  • CD27 receptor binding Source: HGNC
  • tumor necrosis factor receptor binding Source: HGNC

Protein-protein interaction databases

BioGridi206030. 1 interaction.
IntActiO54926. 1 interaction.
STRINGi10090.ENSMUSP00000021728.

Structurei

3D structure databases

ProteinModelPortaliO54926.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 5520Interaction with BCL2L1 isoform Bcl-x(L) and inhibition of BCL2L1 anti-apoptotic activityBy similarityAdd
BLAST

Phylogenomic databases

eggNOGiENOG410IZ95. Eukaryota.
ENOG4112463. LUCA.
GeneTreeiENSGT00390000004842.
HOGENOMiHOG000253914.
HOVERGENiHBG044526.
InParanoidiO54926.
OMAiRALCGQC.
OrthoDBiEOG7K6PW9.
PhylomeDBiO54926.
TreeFamiTF332962.

Family and domain databases

InterProiIPR022773. Siva_cd27-bd.
[Graphical view]
PANTHERiPTHR14365. PTHR14365. 1 hit.
PfamiPF05458. Siva. 1 hit.
[Graphical view]
PIRSFiPIRSF038096. Siva_cd27_bd. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54926-1) [UniParc]FASTAAdd to basket

Also known as: Siva-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKRSCPFAD AAPLQLKVHV GLKELSHGVF AERYSREVFE RTKQLLFQGA
60 70 80 90 100
RAYRDHISSE DCSVNHLQES LKSGVVGAPQ PARGQMLIGP DGRLTRCQAQ
110 120 130 140 150
ASEGGLPRTA PIACSSCMRS VDGKAVCSQC ERALCGQCVY TCWGCGALAC
160 170
VLCGLADYAD DGEKTLCTSC AMFEA
Length:175
Mass (Da):18,770
Last modified:June 1, 1998 - v1
Checksum:i1E132C1580ABE3F2
GO
Isoform 2 (identifier: O54926-2) [UniParc]FASTAAdd to basket

Also known as: Siva-2

The sequence of this isoform differs from the canonical sequence as follows:
     40-104: Missing.

Note: Mouse isoform 2 has been shown to have no pro-apoptotic activity.1 Publication
Show »
Length:110
Mass (Da):11,737
Checksum:iD1B83DA9596503F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1515MPKRS…AAPLQ → MAKGRWRLAEAGAVE in BAB27029 (PubMed:16141072).CuratedAdd
BLAST
Sequence conflicti126 – 1261V → A in AAH12279 (PubMed:15489334).Curated
Sequence conflicti142 – 1421C → S in AAD50060 (PubMed:10597319).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei40 – 10465Missing in isoform 2. 1 PublicationVSP_007526Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033115 Genomic DNA. Translation: AAC04706.1.
AF033113 mRNA. Translation: AAD50059.1.
AF033112 mRNA. Translation: AAD50058.1.
AF033114 mRNA. Translation: AAD50060.1.
AK010562 mRNA. Translation: BAB27029.1.
AK017570 mRNA. Translation: BAB30811.1.
AK153115 mRNA. Translation: BAE31732.1.
BC012279 mRNA. Translation: AAH12279.1.
CCDSiCCDS26193.1. [O54926-1]
CCDS49189.1. [O54926-2]
RefSeqiNP_001155209.1. NM_001161737.1. [O54926-2]
NP_038957.2. NM_013929.2. [O54926-1]
UniGeneiMm.289812.

Genome annotation databases

EnsembliENSMUST00000021728; ENSMUSP00000021728; ENSMUSG00000064326. [O54926-1]
ENSMUST00000109755; ENSMUSP00000105377; ENSMUSG00000064326. [O54926-2]
GeneIDi30954.
KEGGimmu:30954.
UCSCiuc007pev.2. mouse. [O54926-1]
uc007pew.2. mouse. [O54926-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033115 Genomic DNA. Translation: AAC04706.1.
AF033113 mRNA. Translation: AAD50059.1.
AF033112 mRNA. Translation: AAD50058.1.
AF033114 mRNA. Translation: AAD50060.1.
AK010562 mRNA. Translation: BAB27029.1.
AK017570 mRNA. Translation: BAB30811.1.
AK153115 mRNA. Translation: BAE31732.1.
BC012279 mRNA. Translation: AAH12279.1.
CCDSiCCDS26193.1. [O54926-1]
CCDS49189.1. [O54926-2]
RefSeqiNP_001155209.1. NM_001161737.1. [O54926-2]
NP_038957.2. NM_013929.2. [O54926-1]
UniGeneiMm.289812.

3D structure databases

ProteinModelPortaliO54926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206030. 1 interaction.
IntActiO54926. 1 interaction.
STRINGi10090.ENSMUSP00000021728.

PTM databases

PhosphoSiteiO54926.

Proteomic databases

EPDiO54926.
MaxQBiO54926.
PaxDbiO54926.
PeptideAtlasiO54926.
PRIDEiO54926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021728; ENSMUSP00000021728; ENSMUSG00000064326. [O54926-1]
ENSMUST00000109755; ENSMUSP00000105377; ENSMUSG00000064326. [O54926-2]
GeneIDi30954.
KEGGimmu:30954.
UCSCiuc007pev.2. mouse. [O54926-1]
uc007pew.2. mouse. [O54926-2]

Organism-specific databases

CTDi10572.
MGIiMGI:1353606. Siva1.

Phylogenomic databases

eggNOGiENOG410IZ95. Eukaryota.
ENOG4112463. LUCA.
GeneTreeiENSGT00390000004842.
HOGENOMiHOG000253914.
HOVERGENiHBG044526.
InParanoidiO54926.
OMAiRALCGQC.
OrthoDBiEOG7K6PW9.
PhylomeDBiO54926.
TreeFamiTF332962.

Miscellaneous databases

PROiO54926.
SOURCEiSearch...

Gene expression databases

BgeeiO54926.
CleanExiMM_SIVA1.
GenevisibleiO54926. MM.

Family and domain databases

InterProiIPR022773. Siva_cd27-bd.
[Graphical view]
PANTHERiPTHR14365. PTHR14365. 1 hit.
PfamiPF05458. Siva. 1 hit.
[Graphical view]
PIRSFiPIRSF038096. Siva_cd27_bd. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both bind to CD27 but differentially transduce apoptosis."
    Yoon Y., Ao Z., Cheng Y., Schlossman S.F., Prasad K.V.S.
    Oncogene 18:7174-7179(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow and Embryonic stem cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary gland.
  4. "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein."
    Prasad K.V.S., Ao Z., Yoon Y., Wu M.X., Rizk M., Jacquot S., Schlossman S.F.
    Proc. Natl. Acad. Sci. U.S.A. 94:6346-6351(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Apoptosis in coxsackievirus B3-caused diseases: interaction between the capsid protein VP2 and the proapoptotic protein siva."
    Henke A., Launhardt H., Klement K., Stelzner A., Zell R., Munder T.
    J. Virol. 74:4284-4290(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "GITR interacts with the pro-apoptotic protein Siva and induces apoptosis."
    Spinicelli S., Nocentini G., Ronchetti S., Krausz L.T., Bianchini R., Riccardi C.
    Cell Death Differ. 9:1382-1384(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TNFRSF18.
  7. "The proapoptotic gene SIVA is a direct transcriptional target for the tumor suppressors p53 and E2F1."
    Fortin A., MacLaurin J.G., Arbour N., Cregan S.P., Kushwaha N., Callaghan S.M., Park D.S., Albert P.R., Slack R.S.
    J. Biol. Chem. 279:28706-28714(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiSIVA_MOUSE
AccessioniPrimary (citable) accession number: O54926
Secondary accession number(s): Q3U6J2
, Q921I8, Q9CWL1, Q9R1Q0, Q9R1Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.