O54924 (EXOC8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exocyst complex component 8

Alternative name(s):
Exocyst complex 84 kDa subunit

Gene names

Name:	Exoc8
Synonyms:	Exo84

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Subunit structure	The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with the GTP-bound form of RALB via its PH domain By similarity. Interacts with RALA via its PH domain. Ref.1 Ref.2
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Cell projection › growth cone. Note: Binds lipids with phosphatidylinositol-3,4,5-trisphosphate groups By similarity. Perinuclear in undifferentiated PC12 cells. Redistributes to growing neurites and growth cones during cell differentiation. Ref.2
Sequence similarities	Belongs to the EXO84 family. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cell projection Cytoplasm
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular protein localization Inferred from mutant phenotype. Source: RGD exocytosis Traceable author statement Ref.1. Source: RGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	exocyst Inferred from direct assay Ref.1. Source: RGD growth cone Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 716

716

Exocyst complex component 8

PRO_0000227552

Regions

Domain

173 – 273

101

PH

Compositional bias

104 – 109

6

Poly-Ala

Amino acid modifications

Modified residue

15

1

Phosphoserine By similarity

Modified residue

313

1

Phosphothreonine By similarity

Experimental info

Mutagenesis

228

1

A → W: Strongly reduces interaction with RALA. Ref.3

Mutagenesis

233

1

K → W: Strongly reduces interaction with RALA. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

716

Helix Strand Turn

Details...

Beta strand

174 – 184

11

Turn

185 – 187

3

Beta strand

190 – 209

20

Beta strand

216 – 223

8

Turn

224 – 226

3

Beta strand

228 – 231

4

Beta strand

238 – 246

9

Beta strand

249 – 254

6

Helix

258 – 278

21

Sequences

	Sequence		Length	Mass (Da)
	O54924 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EE6945C7F25B18AC	FASTA	716	81,043
	10 20 30 40 50 60 MSDSGASRLR RQLESGGFEA RLYVKQLSQQ SDGDRDLQEH RQRVQALAEE TAQNLKRNVY 70 80 90 100 110 120 QNYRQFIETA REISYLESEM YQLSHLLTEQ KSSLESIPLA LLPAAAAGAS TGEDTAGAGP 130 140 150 160 170 180 RERGAAQAGF LPGPAGVPRE GPGTGEEGKQ RTLTTLLEKV EGCRDLLETP GQYLVYNGDL 190 200 210 220 230 240 VEYEADHMAQ LQRVHGFLMN DCLLVATWLP QRRGMYRYNA LYPLDRLAVV NVKDNPPMKD 250 260 270 280 290 300 MFKLLMFPES RIFQAENAKI KREWLEVLEE TKRALSDKRR REQEEAAALR APPPVTSKGS 310 320 330 340 350 360 NPFEDEAEEE LATPEAEEEK VDLSMEWIQE LPEDLDVCIA QRDFEGAVDL LDKLNHYLED 370 380 390 400 410 420 KPSPPSVKEL RAKVDERVRQ LTEVLVFELS PDRSLRGGPK ATRRAVSQLI RLGQCTKACE 430 440 450 460 470 480 LFLRNRAAAV HTAIRQLRIE GATLLYIHKL CHVFFTSLLE TAREFETDFA GTDSGCYSAF 490 500 510 520 530 540 VVWARSAMGM FVDAFSKQVF DSKESLSTAA ECVKVAKEHC QQLGEIGLDL TFIIHALLVK 550 560 570 580 590 600 DIQGALLSYK EIIIEATKHR NSEEMWRRMN LMTPEALGKL KEEMRSCGVS NFEQYTGDDC 610 620 630 640 650 660 WVNLSYTVVA FTKQTMGFLE EALKLYFPEL HMVLLESLVE VILVAVQHVD YSLRCEQDPE 670 680 690 700 710 KKTFIRQNAS FLYDTVLPVV ERRFEEGVGK PAKQLQDLRN ASRLLRVNPE STTSVV

References

[1]	"Subunit structure of the mammalian exocyst complex." Kee Y., Yoo J.-S., Hazuka C.D., Peterson K.E., Hsu S.-C., Scheller R.H. Proc. Natl. Acad. Sci. U.S.A. 94:14438-14443(1997) [PubMed: 9405631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBUNIT. Tissue: Brain.
[2]	"Immunological characterization of exocyst complex subunits in cell differentiation." Wang S., Hsu S.C. Hybrid. Hybridomics 22:159-164(2003) [PubMed: 12954101] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT.
[3]	"Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase." Jin R., Junutula J.R., Matern H.T., Ervin K.E., Scheller R.H., Brunger A.T. EMBO J. 24:2064-2074(2005) [PubMed: 15920473] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 167-286 IN COMPLEX WITH RALA, MUTAGENESIS OF ALA-228 AND LYS-233.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF032669 mRNA. Translation: AAC01581.1.

IPI

IPI00199846.

PIR

T09222.

RefSeq

NP_620612.1. NM_139043.1.

UniGene

Rn.10901.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZC3	X-ray	2.00	B/D	167-279	[»]
1ZC4	X-ray	2.50	B/D	167-286	[»]

ProteinModelPortal

O54924.

SMR

O54924. Positions 171-283.

ModBase

Search...

Protein-protein interaction databases

STRING

O54924.

PTM databases

PhosphoSite

O54924.

Proteomic databases

PRIDE

O54924.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000026757; ENSRNOP00000026757; ENSRNOG00000019766.

GeneID

245709.

KEGG

rno:245709.

UCSC

NM_139043. rat.

Organism-specific databases

CTD

149371.

RGD

620245. Exoc8.

Phylogenomic databases

eggNOG

maNOG11263.

GeneTree

ENSGT00390000015936.

HOVERGEN

HBG081489.

InParanoid

O54924.

OMA

EMKSCGV.

OrthoDB

EOG4F4S9J.

PhylomeDB

O54924.

Gene expression databases

ArrayExpress

O54924.

Genevestigator

O54924.

GermOnline

ENSRNOG00000019766. Rattus norvegicus.

Family and domain databases

InterPro

IPR016159. Cullin_repeat-like_dom.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR014812. Vps51.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF08700. Vps51. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
[Graphical view]

SUPFAM

SSF74788. Cullin_repeat-like. 1 hit.

PROSITE

PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

623139.

Entry information

Entry name

EXOC8_RAT

Accession

Primary (citable) accession number: O54924

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program