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Protein

Bcl-2-like protein 11

Gene

Bcl2l11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis and anoikis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.1 Publication

GO - Molecular functioni

  • microtubule binding Source: MGI

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic process involved in embryonic digit morphogenesis Source: MGI
  • B cell apoptotic process Source: MGI
  • B cell homeostasis Source: MGI
  • brain development Source: Ensembl
  • cell-matrix adhesion Source: MGI
  • cellular process regulating host cell cycle in response to virus Source: MGI
  • developmental pigmentation Source: MGI
  • ear development Source: MGI
  • extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • leukocyte homeostasis Source: MGI
  • lymphocyte homeostasis Source: MGI
  • male gonad development Source: MGI
  • mammary gland development Source: MGI
  • myeloid cell homeostasis Source: MGI
  • odontogenesis of dentin-containing tooth Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of apoptotic process by virus Source: MGI
  • positive regulation of cell cycle Source: MGI
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • positive regulation of fibroblast apoptotic process Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  • positive regulation of neuron apoptotic process Source: MGI
  • positive regulation of protein homooligomerization Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: MGI
  • post-embryonic development Source: MGI
  • post-embryonic organ morphogenesis Source: MGI
  • regulation of apoptotic process Source: MGI
  • regulation of developmental pigmentation Source: MGI
  • regulation of organ growth Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • spermatogenesis Source: MGI
  • spleen development Source: MGI
  • T cell homeostasis Source: MGI
  • thymocyte apoptotic process Source: MGI
  • thymus development Source: MGI
  • tube formation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_307563. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
REACT_336827. Activation of BIM and translocation to mitochondria.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-like protein 11
Short name:
Bcl2-L-11
Alternative name(s):
Bcl2-interacting mediator of cell death
Gene namesi
Name:Bcl2l11
Synonyms:Bim
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1197519. Bcl2l11.

Subcellular locationi

GO - Cellular componenti

  • BIM-BCL-2 complex Source: MGI
  • BIM-BCL-xl complex Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extrinsic component of membrane Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • microtubule Source: Ensembl
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551S → A: Loss of TRIM2-binding; when associated with A-65 and A-73. 1 Publication
Mutagenesisi65 – 651S → A: Loss of TRIM2-binding; when associated with A-55 and A-73. 1 Publication
Mutagenesisi73 – 731S → A: Loss of TRIM2-binding; when associated with A-55 and A-65. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Bcl-2-like protein 11PRO_0000143110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine; by MAPK1 Publication

Post-translational modificationi

Phosphorylation at Ser-65 by MAPK1/MAPK3 induces interaction with TRIM2, followed by ubiquitination.1 Publication
Ubiquitinated by TRIM2 following phosphorylation by MAPK1/MAPK3, leading to degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO54918.
PRIDEiO54918.

PTM databases

PhosphoSiteiO54918.

Expressioni

Tissue specificityi

Expressed in a number of B- and T-lymphoid cell lines.1 Publication

Inductioni

By ER stress.1 Publication

Gene expression databases

BgeeiO54918.
CleanExiMM_BCL2L11.
ExpressionAtlasiO54918. baseline and differential.
GenevisibleiO54918. MM.

Interactioni

Subunit structurei

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Interacts with Bax (in vitro). Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L). Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation. Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bcl2P104172EBI-526076,EBI-526314
GNB2L1P632442EBI-526076,EBI-296739From a different organism.
Mcl1P972874EBI-526067,EBI-707292
Prkar1aQ9DBC72EBI-526076,EBI-645677

Protein-protein interaction databases

BioGridi198352. 13 interactions.
DIPiDIP-29214N.
IntActiO54918. 16 interactions.
MINTiMINT-89451.
STRINGi10090.ENSMUSP00000105970.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi142 – 16827Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtiDP00518.
ProteinModelPortaliO54918.
SMRiO54918. Positions 139-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 16015BH3Add
BLAST

Domaini

The BH3 motif is required for Bcl-2 binding and cytotoxicity.

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiNOG40262.
GeneTreeiENSGT00390000003178.
HOGENOMiHOG000231637.
InParanoidiO54918.
OMAiAEDHPQM.
PhylomeDBiO54918.
TreeFamiTF335898.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform BimEL (identifier: O54918-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QGNPDGEGDR
60 70 80 90 100
CPHGSPQGPL APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS
110 120 130 140 150
PAPMSCDKST QTPSPPCQAF NHYLSAMASI RQSQEEPEDL RPEIRIAQEL
160 170 180 190
RRIGDEFNET YTRRVFANDY REAEDHPQMV ILQLLRFIFR LVWRRH
Length:196
Mass (Da):22,067
Last modified:June 1, 1998 - v1
Checksum:i531C176E5F1AC9AA
GO
Isoform BimL (identifier: O54918-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Show »
Length:140
Mass (Da):16,205
Checksum:iB127B40A190DA923
GO
Isoform BimS (identifier: O54918-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Show »
Length:110
Mass (Da):12,954
Checksum:i67AB421AC1E4D2CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 12786Missing in isoform BimS. CuratedVSP_000537Add
BLAST
Alternative sequencei42 – 9756Missing in isoform BimL. CuratedVSP_000536Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSiCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqiNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneiMm.141083.
Mm.453214.

Genome annotation databases

EnsembliENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneIDi12125.
UCSCiuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSiCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqiNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneiMm.141083.
Mm.453214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtiDP00518.
ProteinModelPortaliO54918.
SMRiO54918. Positions 139-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198352. 13 interactions.
DIPiDIP-29214N.
IntActiO54918. 16 interactions.
MINTiMINT-89451.
STRINGi10090.ENSMUSP00000105970.

PTM databases

PhosphoSiteiO54918.

Proteomic databases

PaxDbiO54918.
PRIDEiO54918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneIDi12125.
UCSCiuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Organism-specific databases

CTDi10018.
MGIiMGI:1197519. Bcl2l11.

Phylogenomic databases

eggNOGiNOG40262.
GeneTreeiENSGT00390000003178.
HOGENOMiHOG000231637.
InParanoidiO54918.
OMAiAEDHPQM.
PhylomeDBiO54918.
TreeFamiTF335898.

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_307563. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
REACT_336827. Activation of BIM and translocation to mitochondria.

Miscellaneous databases

ChiTaRSiBcl2l11. mouse.
EvolutionaryTraceiO54918.
NextBioi280451.
PROiO54918.
SOURCEiSearch...

Gene expression databases

BgeeiO54918.
CleanExiMM_BCL2L11.
ExpressionAtlasiO54918. baseline and differential.
GenevisibleiO54918. MM.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bim: a novel member of the Bcl-2 family that promotes apoptosis."
    O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S.
    EMBO J. 17:384-395(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BIMEL).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1."
    Maurer U., Charvet C., Wagman A.S., Dejardin E., Green D.R.
    Mol. Cell 21:749-760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MCL1.
  5. "Neuronal apoptosis induced by endoplasmic reticulum stress is regulated by ATF4-CHOP-mediated induction of the Bcl-2 homology 3-only member PUMA."
    Galehdar Z., Swan P., Fuerth B., Callaghan S.M., Park D.S., Cregan S.P.
    J. Neurosci. 30:16938-16948(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
    Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
    J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLL1; TRIM2 AND YWHAZ, UBIQUITINATION, PHOSPHORYLATION AT SER-65, MUTAGENESIS OF SER-55; SER-65 AND SER-73.
  7. "The structure of a Bcl-xL/Bim fragment complex: implications for Bim function."
    Liu X., Dai S., Zhu Y., Marrack P., Kappler J.W.
    Immunity 19:341-352(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 139-171 IN COMPLEX WITH BCL2L1 ISOFORM BCL-XL, IDENTIFICATION IN A COMPLEX WITH DYNLL1 AND BCL-XL, INTERACTION WITH BAX.

Entry informationi

Entry nameiB2L11_MOUSE
AccessioniPrimary (citable) accession number: O54918
Secondary accession number(s): A2ALQ6, O54919, O54920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 1998
Last modified: July 22, 2015
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.