Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O54918

- B2L11_MOUSE

UniProt

O54918 - B2L11_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Bcl-2-like protein 11

Gene

Bcl2l11

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Induces apoptosis and anoikis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.1 Publication

GO - Molecular functioni

  1. microtubule binding Source: MGI

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  2. apoptotic process involved in embryonic digit morphogenesis Source: MGI
  3. B cell apoptotic process Source: MGI
  4. B cell homeostasis Source: MGI
  5. brain development Source: Ensembl
  6. cell-matrix adhesion Source: MGI
  7. cellular process regulating host cell cycle in response to virus Source: MGI
  8. developmental pigmentation Source: MGI
  9. ear development Source: MGI
  10. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  11. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  12. in utero embryonic development Source: MGI
  13. kidney development Source: MGI
  14. leukocyte homeostasis Source: MGI
  15. lymphocyte homeostasis Source: MGI
  16. male gonad development Source: MGI
  17. mammary gland development Source: MGI
  18. myeloid cell homeostasis Source: MGI
  19. odontogenesis of dentin-containing tooth Source: MGI
  20. positive regulation of apoptotic process Source: MGI
  21. positive regulation of apoptotic process by virus Source: MGI
  22. positive regulation of cell cycle Source: MGI
  23. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  24. positive regulation of fibroblast apoptotic process Source: Ensembl
  25. positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
  26. positive regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  27. positive regulation of neuron apoptotic process Source: MGI
  28. positive regulation of protein homooligomerization Source: Ensembl
  29. positive regulation of release of cytochrome c from mitochondria Source: Ensembl
  30. post-embryonic development Source: MGI
  31. post-embryonic organ morphogenesis Source: MGI
  32. regulation of apoptotic process Source: MGI
  33. regulation of developmental pigmentation Source: MGI
  34. regulation of organ growth Source: MGI
  35. response to endoplasmic reticulum stress Source: UniProtKB
  36. spermatogenesis Source: MGI
  37. spleen development Source: MGI
  38. T cell homeostasis Source: MGI
  39. thymocyte apoptotic process Source: MGI
  40. thymus development Source: MGI
  41. tube formation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_209641. NRAGE signals death through JNK.
REACT_223399. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-like protein 11
Short name:
Bcl2-L-11
Alternative name(s):
Bcl2-interacting mediator of cell death
Gene namesi
Name:Bcl2l11
Synonyms:Bim
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1197519. Bcl2l11.

Subcellular locationi

Membrane 1 Publication; Peripheral membrane protein 1 Publication. Mitochondrion By similarity
Note: Associated with intracytoplasmic membranes.

GO - Cellular componenti

  1. BIM-BCL-2 complex Source: Ensembl
  2. BIM-BCL-xl complex Source: Ensembl
  3. cytoplasm Source: MGI
  4. cytosol Source: Ensembl
  5. extrinsic component of membrane Source: MGI
  6. intracellular membrane-bounded organelle Source: MGI
  7. microtubule Source: Ensembl
  8. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551S → A: Loss of TRIM2-binding; when associated with A-65 and A-73. 1 Publication
Mutagenesisi65 – 651S → A: Loss of TRIM2-binding; when associated with A-55 and A-73. 1 Publication
Mutagenesisi73 – 731S → A: Loss of TRIM2-binding; when associated with A-55 and A-65. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Bcl-2-like protein 11PRO_0000143110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine; by MAPK1 Publication

Post-translational modificationi

Phosphorylation at Ser-65 by MAPK1/MAPK3 induces interaction with TRIM2, followed by ubiquitination.1 Publication
Ubiquitinated by TRIM2 following phosphorylation by MAPK1/MAPK3, leading to degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO54918.
PRIDEiO54918.

PTM databases

PhosphoSiteiO54918.

Expressioni

Tissue specificityi

Expressed in a number of B- and T-lymphoid cell lines.1 Publication

Inductioni

By ER stress.1 Publication

Gene expression databases

BgeeiO54918.
CleanExiMM_BCL2L11.
ExpressionAtlasiO54918. baseline and differential.
GenevestigatoriO54918.

Interactioni

Subunit structurei

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Interacts with Bax (in vitro). Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L). Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation. Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bcl2P104172EBI-526076,EBI-526314
GNB2L1P632442EBI-526076,EBI-296739From a different organism.
Mcl1P972874EBI-526067,EBI-707292
Prkar1aQ9DBC72EBI-526076,EBI-645677

Protein-protein interaction databases

BioGridi198352. 12 interactions.
DIPiDIP-29214N.
IntActiO54918. 16 interactions.
MINTiMINT-89451.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi142 – 16827

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtiDP00518.
ProteinModelPortaliO54918.
SMRiO54918. Positions 139-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 16015BH3Add
BLAST

Domaini

The BH3 motif is required for Bcl-2 binding and cytotoxicity.

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiNOG40262.
GeneTreeiENSGT00390000003178.
HOGENOMiHOG000231637.
InParanoidiO54918.
KOiK16341.
OMAiIRLVWRM.
PhylomeDBiO54918.
TreeFamiTF335898.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform BimEL (identifier: O54918-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QGNPDGEGDR
60 70 80 90 100
CPHGSPQGPL APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS
110 120 130 140 150
PAPMSCDKST QTPSPPCQAF NHYLSAMASI RQSQEEPEDL RPEIRIAQEL
160 170 180 190
RRIGDEFNET YTRRVFANDY REAEDHPQMV ILQLLRFIFR LVWRRH
Length:196
Mass (Da):22,067
Last modified:June 1, 1998 - v1
Checksum:i531C176E5F1AC9AA
GO
Isoform BimL (identifier: O54918-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Show »
Length:140
Mass (Da):16,205
Checksum:iB127B40A190DA923
GO
Isoform BimS (identifier: O54918-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Show »
Length:110
Mass (Da):12,954
Checksum:i67AB421AC1E4D2CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 12786Missing in isoform BimS. CuratedVSP_000537Add
BLAST
Alternative sequencei42 – 9756Missing in isoform BimL. CuratedVSP_000536Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSiCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqiNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneiMm.141083.
Mm.453214.

Genome annotation databases

EnsembliENSMUST00000019281; ENSMUSP00000019281; ENSMUSG00000027381. [O54918-3]
ENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000103211; ENSMUSP00000099500; ENSMUSG00000027381. [O54918-3]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneIDi12125.
KEGGimmu:12125.
UCSCiuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF032459 mRNA. Translation: AAC40029.1 .
AF032460 mRNA. Translation: AAC40030.1 .
AF032461 mRNA. Translation: AAC40031.1 .
AL805950 Genomic DNA. Translation: CAM14745.1 .
BC019556 mRNA. Translation: AAH19556.1 .
CCDSi CCDS16712.1. [O54918-3 ]
CCDS16713.1. [O54918-1 ]
CCDS16714.1. [O54918-2 ]
RefSeqi NP_033884.1. NM_009754.3. [O54918-3 ]
NP_997563.1. NM_207680.2. [O54918-1 ]
NP_997564.1. NM_207681.2. [O54918-2 ]
UniGenei Mm.141083.
Mm.453214.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PQ1 X-ray 1.65 B 139-171 [» ]
DisProti DP00518.
ProteinModelPortali O54918.
SMRi O54918. Positions 139-171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198352. 12 interactions.
DIPi DIP-29214N.
IntActi O54918. 16 interactions.
MINTi MINT-89451.

PTM databases

PhosphoSitei O54918.

Proteomic databases

PaxDbi O54918.
PRIDEi O54918.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019281 ; ENSMUSP00000019281 ; ENSMUSG00000027381 . [O54918-3 ]
ENSMUST00000103210 ; ENSMUSP00000099499 ; ENSMUSG00000027381 . [O54918-2 ]
ENSMUST00000103211 ; ENSMUSP00000099500 ; ENSMUSG00000027381 . [O54918-3 ]
ENSMUST00000110341 ; ENSMUSP00000105970 ; ENSMUSG00000027381 . [O54918-1 ]
GeneIDi 12125.
KEGGi mmu:12125.
UCSCi uc008mgh.1. mouse. [O54918-1 ]
uc008mgk.1. mouse. [O54918-2 ]

Organism-specific databases

CTDi 10018.
MGIi MGI:1197519. Bcl2l11.

Phylogenomic databases

eggNOGi NOG40262.
GeneTreei ENSGT00390000003178.
HOGENOMi HOG000231637.
InParanoidi O54918.
KOi K16341.
OMAi IRLVWRM.
PhylomeDBi O54918.
TreeFami TF335898.

Enzyme and pathway databases

Reactomei REACT_209641. NRAGE signals death through JNK.
REACT_223399. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.

Miscellaneous databases

EvolutionaryTracei O54918.
NextBioi 280451.
PROi O54918.
SOURCEi Search...

Gene expression databases

Bgeei O54918.
CleanExi MM_BCL2L11.
ExpressionAtlasi O54918. baseline and differential.
Genevestigatori O54918.

Family and domain databases

InterProi IPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view ]
Pfami PF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bim: a novel member of the Bcl-2 family that promotes apoptosis."
    O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S.
    EMBO J. 17:384-395(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BIMEL).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1."
    Maurer U., Charvet C., Wagman A.S., Dejardin E., Green D.R.
    Mol. Cell 21:749-760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MCL1.
  5. "Neuronal apoptosis induced by endoplasmic reticulum stress is regulated by ATF4-CHOP-mediated induction of the Bcl-2 homology 3-only member PUMA."
    Galehdar Z., Swan P., Fuerth B., Callaghan S.M., Park D.S., Cregan S.P.
    J. Neurosci. 30:16938-16948(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
    Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
    J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLL1; TRIM2 AND YWHAZ, UBIQUITINATION, PHOSPHORYLATION AT SER-65, MUTAGENESIS OF SER-55; SER-65 AND SER-73.
  7. "The structure of a Bcl-xL/Bim fragment complex: implications for Bim function."
    Liu X., Dai S., Zhu Y., Marrack P., Kappler J.W.
    Immunity 19:341-352(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 139-171 IN COMPLEX WITH BCL2L1 ISOFORM BCL-XL, IDENTIFICATION IN A COMPLEX WITH DYNLL1 AND BCL-XL, INTERACTION WITH BAX.

Entry informationi

Entry nameiB2L11_MOUSE
AccessioniPrimary (citable) accession number: O54918
Secondary accession number(s): A2ALQ6, O54919, O54920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3