Reviewed, UniProtKB/Swiss-Prot O54918 (B2L11_MOUSE)

Names and origin

Protein names

Recommended name:
    Bcl-2-like protein 11
      Short name=Bcl2-L-11
Alternative name(s):
    Bcl2-interacting mediator of cell death

Gene names

Name:	Bcl2l11
Synonyms:	Bim

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Induces apoptosis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent. Ref.1
Subunit structure	Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK By similarity. Interacts with Bax (in vitro). Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L).
Subcellular location	Membrane; Peripheral membrane protein. Mitochondrion By similarity. Note: Associated with intracytoplasmic membranes.
Tissue specificity	Expressed in a number of B- and T-lymphoid cell lines. Ref.1
Domain	The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	B cell apoptosis Inferred from genetic interaction. Source: MGI B cell homeostasis Inferred from mutant phenotype. Source: MGI T cell homeostasis Inferred from mutant phenotype. Source: MGI cell-matrix adhesion Inferred from mutant phenotype. Source: MGI ear development Inferred from genetic interaction. Source: MGI in utero embryonic development Inferred from genetic interaction. Source: MGI kidney development Inferred from genetic interaction. Source: MGI male gonad development Inferred from genetic interaction. Source: MGI mammary gland development Inferred from mutant phenotype. Source: MGI myeloid cell homeostasis Inferred from genetic interaction. Source: MGI odontogenesis of dentine-containing tooth Inferred from genetic interaction. Source: MGI pigmentation during development Inferred from genetic interaction. Source: MGI positive regulation of apoptosis Inferred from mutant phenotype. Source: MGI post-embryonic organ morphogenesis Inferred from mutant phenotype. Source: MGI regulation of organ growth Inferred from mutant phenotype. Source: MGI regulation of pigmentation during development Inferred from genetic interaction. Source: MGI spermatogenesis Inferred from genetic interaction. Source: MGI spleen development Inferred from genetic interaction. Source: MGI thymus development Inferred from genetic interaction. Source: MGI tube lumen formation Inferred from mutant phenotype. Source: MGI
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: MGI peripheral to membrane of membrane fraction Inferred from direct assay. Source: MGI
Molecular function	microtubule binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2	P10415	1	EBI-526067,EBI-77694	From a different organism.
Bcl2a1	Q07440	1	EBI-526067,EBI-707754
BCL2L1	Q07817-1	1	EBI-526067,EBI-287195	From a different organism.
Bcl2l1	Q64373	1	EBI-526067,EBI-526361
BCL2L2	Q92843	1	EBI-526067,EBI-707714	From a different organism.
DYNLRB1	Q9NP97	1	EBI-526080,EBI-372128	From a different organism.
DYNLRB1	Q9NP97	1	EBI-526076,EBI-372128	From a different organism.
GNB2L1	P63244	2	EBI-526076,EBI-296739	From a different organism.
Mcl1	P97287	1	EBI-526067,EBI-707292
Ywhab	Q9CQV8	1	EBI-526067,EBI-771608

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform BimEL (identifier: O54918-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform BimL (identifier: O54918-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Isoform BimS (identifier: O54918-3)

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

Bcl-2-like protein 11

PRO_0000143110

Regions

Motif

146 – 160

15

BH3

Amino acid modifications

Modified residue

73

1

Phosphoserine By similarity

Natural variations

Alternative sequence

42 – 127

86

Missing in isoform BimS.

VSP_000537

Alternative sequence

42 – 97

56

Missing in isoform BimL.

VSP_000536

Secondary structure

1

.

.

.

196

Helix Strand Turn

Details...

Helix

142 – 168

27

Sequences

	Sequence		Length	Mass (Da)
	Isoform BimEL [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 531C176E5F1AC9AA	FASTA	196	22,067
	10 20 30 40 50 60 MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QGNPDGEGDR CPHGSPQGPL 70 80 90 100 110 120 APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS PAPMSCDKST QTPSPPCQAF 130 140 150 160 170 180 NHYLSAMASI RQSQEEPEDL RPEIRIAQEL RRIGDEFNET YTRRVFANDY REAEDHPQMV 190 ILQLLRFIFR LVWRRH
	Isoform BimL. Checksum: B127B40A190DA923	FASTA	140	16,205
	10 20 30 40 50 60 MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QDRSPAPMSC DKSTQTPSPP 70 80 90 100 110 120 CQAFNHYLSA MASIRQSQEE PEDLRPEIRI AQELRRIGDE FNETYTRRVF ANDYREAEDH 130 140 PQMVILQLLR FIFRLVWRRH
	Isoform BimS. Checksum: 67AB421AC1E4D2CA	FASTA	110	12,954
	10 20 30 40 50 60 MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QASIRQSQEE PEDLRPEIRI 70 80 90 100 110 AQELRRIGDE FNETYTRRVF ANDYREAEDH PQMVILQLLR FIFRLVWRRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bim: a novel member of the Bcl-2 family that promotes apoptosis." O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S. EMBO J. 17:384-395(1998) [PubMed: 9430630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BIMEL). Strain: FVB/N. Tissue: Mammary tumor.
[3]	"The structure of a Bcl-xL/Bim fragment complex: implications for Bim function." Liu X., Dai S., Zhu Y., Marrack P., Kappler J.W. Immunity 19:341-352(2003) [PubMed: 14499110] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 139-171 IN COMPLEX WITH BCL2L1 ISOFORM BCL-XL, IDENTIFICATION IN A COMPLEX WITH DYNLL1 AND BCL-XL, INTERACTION WITH BAX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
BC019556 mRNA. Translation: AAH19556.1.

IPI

IPI00119799.
IPI00225688.
IPI00225689.

RefSeq

NP_033884.1.
NP_997563.1.

UniGene

Mm.141083
Mm.453214

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PQ1	X-ray	1.65	B	139-171	[»]

DisProt

DP00518.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29214N.

IntAct

O54918. 17 interactions.

PTM databases

PhosphoSite

O54918.

Genome annotation databases

Ensembl

ENSMUSG00000027381. Mus musculus. [Contig view]

GeneID

12125.

KEGG

mmu:12125.

Organism-specific databases

MGI

MGI:1197519. Bcl2l11.

Phylogenomic databases

HOGENOM

O54918.

HOVERGEN

O54918.

OMA

O54918. FANDYRE.

Gene expression databases

ArrayExpress

O54918.

Bgee

O54918.

CleanEx

MM_BCL2L11.

GermOnline

ENSMUSG00000027381. Mus musculus.

Family and domain databases

InterPro

IPR017288. Apoptosis_Bcl-2-like_11.
IPR009616. Apoptosis_Bim.
IPR014771. Apoptosis_Bim_N.
IPR015040. Bcl-x_interacting.
IPR000712. Bcl2_BH.
[Graphical view]

PANTHER

PTHR12044:SF1. Bim_N. 1 hit.

Pfam

PF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]

PIRSF

PIRSF037827. Bcl-2-like_p11. 1 hit.

PROSITE

PS01259. BH3. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

280451.

SOURCE

Search...

Entry information

Entry name

B2L11_MOUSE

Accession

Primary (citable) accession number: O54918
Secondary accession number(s): O54919, O54920

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)