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Protein

Bcl-2-like protein 11

Gene

Bcl2l11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis and anoikis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.1 Publication

GO - Molecular functioni

  • dynein binding Source: MGI
  • microtubule binding Source: MGI

GO - Biological processi

  • apoptotic process involved in embryonic digit morphogenesis Source: MGI
  • B cell apoptotic process Source: MGI
  • B cell homeostasis Source: MGI
  • brain development Source: Ensembl
  • cell-matrix adhesion Source: MGI
  • cellular process regulating host cell cycle in response to virus Source: MGI
  • developmental pigmentation Source: MGI
  • ear development Source: MGI
  • extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • leukocyte homeostasis Source: MGI
  • lymphocyte homeostasis Source: MGI
  • male gonad development Source: MGI
  • mammary gland development Source: MGI
  • myeloid cell homeostasis Source: MGI
  • odontogenesis of dentin-containing tooth Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of apoptotic process by virus Source: MGI
  • positive regulation of cell cycle Source: MGI
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • positive regulation of fibroblast apoptotic process Source: MGI
  • positive regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  • positive regulation of neuron apoptotic process Source: MGI
  • positive regulation of protein homooligomerization Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: MGI
  • post-embryonic animal organ morphogenesis Source: MGI
  • post-embryonic development Source: MGI
  • regulation of apoptotic process Source: MGI
  • regulation of developmental pigmentation Source: MGI
  • regulation of organ growth Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • spermatogenesis Source: MGI
  • spleen development Source: MGI
  • T cell homeostasis Source: MGI
  • thymocyte apoptotic process Source: MGI
  • thymus development Source: MGI
  • tube formation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-111446. Activation of BIM and translocation to mitochondria.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-MMU-193648. NRAGE signals death through JNK.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-like protein 11
Short name:
Bcl2-L-11
Alternative name(s):
Bcl2-interacting mediator of cell death
Gene namesi
Name:Bcl2l11
Synonyms:Bim
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1197519. Bcl2l11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extrinsic component of membrane Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55S → A: Loss of TRIM2-binding; when associated with A-65 and A-73. 1 Publication1
Mutagenesisi65S → A: Loss of TRIM2-binding; when associated with A-55 and A-73. 1 Publication1
Mutagenesisi73S → A: Loss of TRIM2-binding; when associated with A-55 and A-65. 1 Publication1
Mutagenesisi150Missing : Loss of MCL1-binding, strong decrease in BCL2L1-binding, no effect on USP27X-binding; when associated with I-153 del. 1 Publication1
Mutagenesisi153Missing : Loss of MCL1-binding, strong decrease in BCL2L1-binding, no effect on USP27X-binding; when associated with L-150 del. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001431101 – 196Bcl-2-like protein 11Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65Phosphoserine; by MAPK2 Publications1
Modified residuei73PhosphoserineBy similarity1
Modified residuei83PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-65 by MAPK1/MAPK3 leads interaction with TRIM2 and ubiquitination, followed by proteasomal degradation (PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (PubMed:27013495).2 Publications
Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO54918.
PeptideAtlasiO54918.
PRIDEiO54918.

PTM databases

iPTMnetiO54918.
PhosphoSitePlusiO54918.

Expressioni

Tissue specificityi

Expressed in a number of B- and T-lymphoid cell lines.1 Publication

Inductioni

By ER stress.1 Publication

Gene expression databases

BgeeiENSMUSG00000027381.
CleanExiMM_BCL2L11.
ExpressionAtlasiO54918. baseline and differential.
GenevisibleiO54918. MM.

Interactioni

Subunit structurei

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins, including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BCL2L2/BCLW (PubMed:16543145, PubMed:27013495, PubMed:14499110). Interacts with BAX (in vitro); this interaction may induce the conformationally active form of BAX (PubMed:14499110). Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L); BH3 integrity is required for BCL2L1-binding (PubMed:21478148, PubMed:27013495, PubMed:14499110). Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation (PubMed:21478148). Interacts (via BH3) with MCL1; this interaction may sequester BCL2L11 and prevent its pro-apoptotic activity (PubMed:16543145, PubMed:27013495). When phosphorylated, isoform BimEL interacts with USP27X; this interaction leads to BCL2L11 deubiquitination and stabilization (PubMed:27013495).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bcl2P104172EBI-526076,EBI-526314
Mcl1P972874EBI-526067,EBI-707292
Prkar1aQ9DBC72EBI-526076,EBI-645677
RACK1P632442EBI-526076,EBI-296739From a different organism.

GO - Molecular functioni

  • dynein binding Source: MGI
  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi198352. 13 interactors.
DIPiDIP-29214N.
IntActiO54918. 16 interactors.
MINTiMINT-89451.
STRINGi10090.ENSMUSP00000105970.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi142 – 168Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtiDP00518.
ProteinModelPortaliO54918.
SMRiO54918.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54918.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 160BH3Add BLAST15

Domaini

The BH3 motif is required for the interaction with Bcl-2 proteins and cytotoxicity.2 Publications

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
HOGENOMiHOG000231637.
InParanoidiO54918.
KOiK16341.
OMAiAEDHPQM.
OrthoDBiEOG091G0XD3.
PhylomeDBiO54918.
TreeFamiTF335898.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform BimEL (identifier: O54918-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QGNPDGEGDR
60 70 80 90 100
CPHGSPQGPL APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS
110 120 130 140 150
PAPMSCDKST QTPSPPCQAF NHYLSAMASI RQSQEEPEDL RPEIRIAQEL
160 170 180 190
RRIGDEFNET YTRRVFANDY REAEDHPQMV ILQLLRFIFR LVWRRH
Length:196
Mass (Da):22,067
Last modified:June 1, 1998 - v1
Checksum:i531C176E5F1AC9AA
GO
Isoform BimL (identifier: O54918-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Show »
Length:140
Mass (Da):16,205
Checksum:iB127B40A190DA923
GO
Isoform BimS (identifier: O54918-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Show »
Length:110
Mass (Da):12,954
Checksum:i67AB421AC1E4D2CA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00053742 – 127Missing in isoform BimS. CuratedAdd BLAST86
Alternative sequenceiVSP_00053642 – 97Missing in isoform BimL. CuratedAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSiCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqiNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneiMm.141083.
Mm.453214.

Genome annotation databases

EnsembliENSMUST00000019281; ENSMUSP00000019281; ENSMUSG00000027381. [O54918-3]
ENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000103211; ENSMUSP00000099500; ENSMUSG00000027381. [O54918-3]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneIDi12125.
KEGGimmu:12125.
UCSCiuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSiCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqiNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneiMm.141083.
Mm.453214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtiDP00518.
ProteinModelPortaliO54918.
SMRiO54918.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198352. 13 interactors.
DIPiDIP-29214N.
IntActiO54918. 16 interactors.
MINTiMINT-89451.
STRINGi10090.ENSMUSP00000105970.

PTM databases

iPTMnetiO54918.
PhosphoSitePlusiO54918.

Proteomic databases

PaxDbiO54918.
PeptideAtlasiO54918.
PRIDEiO54918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019281; ENSMUSP00000019281; ENSMUSG00000027381. [O54918-3]
ENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000103211; ENSMUSP00000099500; ENSMUSG00000027381. [O54918-3]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneIDi12125.
KEGGimmu:12125.
UCSCiuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Organism-specific databases

CTDi10018.
MGIiMGI:1197519. Bcl2l11.

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
HOGENOMiHOG000231637.
InParanoidiO54918.
KOiK16341.
OMAiAEDHPQM.
OrthoDBiEOG091G0XD3.
PhylomeDBiO54918.
TreeFamiTF335898.

Enzyme and pathway databases

ReactomeiR-MMU-111446. Activation of BIM and translocation to mitochondria.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-MMU-193648. NRAGE signals death through JNK.

Miscellaneous databases

ChiTaRSiBcl2l11. mouse.
EvolutionaryTraceiO54918.
PROiO54918.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027381.
CleanExiMM_BCL2L11.
ExpressionAtlasiO54918. baseline and differential.
GenevisibleiO54918. MM.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB2L11_MOUSE
AccessioniPrimary (citable) accession number: O54918
Secondary accession number(s): A2ALQ6, O54919, O54920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.