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O54918 (B2L11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bcl-2-like protein 11

Short name=Bcl2-L-11
Alternative name(s):
Bcl2-interacting mediator of cell death
Gene names
Name:Bcl2l11
Synonyms:Bim
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Induces apoptosis and anoikis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent. Ref.1

Subunit structure

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L) BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Interacts with Bax (in vitro). Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L) Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation. Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity. Ref.4 Ref.6 Ref.7

Subcellular location

Membrane; Peripheral membrane protein. Mitochondrion By similarity. Note: Associated with intracytoplasmic membranes. Ref.1

Tissue specificity

Expressed in a number of B- and T-lymphoid cell lines. Ref.1

Induction

By ER stress. Ref.5

Domain

The BH3 motif is required for Bcl-2 binding and cytotoxicity.

Post-translational modification

Phosphorylation at Ser-65 by MAPK1/MAPK3 induces interaction with TRIM2, followed by ubiquitination.

Ubiquitinated by TRIM2 following phosphorylation by MAPK1/MAPK3, leading to degradation by the proteasome. Ref.6

Sequence similarities

Belongs to the Bcl-2 family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentMembrane
Mitochondrion
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell apoptotic process

Inferred from genetic interaction PubMed 16055554. Source: MGI

B cell homeostasis

Inferred from mutant phenotype PubMed 16055554PubMed 16270031. Source: MGI

T cell homeostasis

Inferred from mutant phenotype PubMed 16270031. Source: MGI

activation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

apoptotic process involved in embryonic digit morphogenesis

Inferred from genetic interaction PubMed 15967824. Source: MGI

brain development

Inferred from electronic annotation. Source: Ensembl

cell-matrix adhesion

Inferred from mutant phenotype PubMed 17276340. Source: MGI

cellular process regulating host cell cycle in response to virus

Inferred from direct assay PubMed 15231831. Source: MGI

developmental pigmentation

Inferred from genetic interaction PubMed 11709185. Source: MGI

ear development

Inferred from genetic interaction PubMed 11709185. Source: MGI

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from mutant phenotype PubMed 15967824. Source: MGI

in utero embryonic development

Inferred from genetic interaction PubMed 16282979. Source: MGI

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from genetic interaction PubMed 15967824. Source: MGI

kidney development

Inferred from genetic interaction PubMed 11709185. Source: MGI

leukocyte homeostasis

Inferred from mutant phenotype PubMed 15967824. Source: MGI

lymphocyte homeostasis

Inferred from mutant phenotype PubMed 11709185. Source: MGI

male gonad development

Inferred from genetic interaction PubMed 16270031. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 17276340. Source: MGI

myeloid cell homeostasis

Inferred from genetic interaction PubMed 15967824. Source: MGI

odontogenesis of dentin-containing tooth

Inferred from genetic interaction PubMed 15967824. Source: MGI

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 16270031PubMed 16832056PubMed 17276340. Source: MGI

positive regulation of apoptotic process by virus

Inferred from direct assay PubMed 15231831. Source: MGI

positive regulation of cell cycle

Inferred from mutant phenotype PubMed 17705137. Source: MGI

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 15967824. Source: MGI

positive regulation of fibroblast apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of intrinsic apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitochondrial membrane permeability involved in apoptotic process

Inferred from genetic interaction PubMed 15967824. Source: MGI

positive regulation of neuron apoptotic process

Inferred from direct assay PubMed 15231831. Source: MGI

positive regulation of protein homooligomerization

Inferred from electronic annotation. Source: Ensembl

positive regulation of release of cytochrome c from mitochondria

Inferred from electronic annotation. Source: Ensembl

post-embryonic development

Inferred from genetic interaction PubMed 11709185. Source: MGI

post-embryonic organ morphogenesis

Inferred from mutant phenotype PubMed 17276340. Source: MGI

regulation of apoptotic process

Inferred from genetic interaction PubMed 15818405. Source: MGI

regulation of developmental pigmentation

Inferred from genetic interaction PubMed 15818405. Source: MGI

regulation of organ growth

Inferred from mutant phenotype PubMed 17276340. Source: MGI

response to endoplasmic reticulum stress

Inferred from direct assay Ref.5. Source: UniProtKB

spermatogenesis

Inferred from genetic interaction PubMed 16270031. Source: MGI

spleen development

Inferred from genetic interaction PubMed 15818405. Source: MGI

thymocyte apoptotic process

Inferred from genetic interaction PubMed 15967824. Source: MGI

thymus development

Inferred from genetic interaction PubMed 15818405. Source: MGI

tube formation

Inferred from mutant phenotype PubMed 17276340. Source: MGI

   Cellular_componentBIM-BCL-2 complex

Inferred from electronic annotation. Source: Ensembl

BIM-BCL-xl complex

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay PubMed 15136728. Source: MGI

cytosol

Inferred from electronic annotation. Source: Ensembl

extrinsic component of membrane

Inferred from direct assay PubMed 15136728. Source: MGI

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 15136728. Source: MGI

microtubule

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from direct assay PubMed 15136728. Source: MGI

   Molecular_functionmicrotubule binding

Inferred from direct assay PubMed 16832056. Source: MGI

protein binding

Inferred from physical interaction PubMed 18420585PubMed 21151042. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Bcl2P104172EBI-526076,EBI-526314
GNB2L1P632442EBI-526076,EBI-296739From a different organism.
Mcl1P972874EBI-526067,EBI-707292
Prkar1aQ9DBC72EBI-526076,EBI-645677

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform BimEL (identifier: O54918-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform BimL (identifier: O54918-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.
Isoform BimS (identifier: O54918-3)

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 196196Bcl-2-like protein 11
PRO_0000143110

Regions

Motif146 – 16015BH3

Amino acid modifications

Modified residue651Phosphoserine; by MAPK Ref.6

Natural variations

Alternative sequence42 – 12786Missing in isoform BimS.
VSP_000537
Alternative sequence42 – 9756Missing in isoform BimL.
VSP_000536

Experimental info

Mutagenesis551S → A: Loss of TRIM2-binding; when associated with A-65 and A-73. Ref.6
Mutagenesis651S → A: Loss of TRIM2-binding; when associated with A-55 and A-73. Ref.6
Mutagenesis731S → A: Loss of TRIM2-binding; when associated with A-55 and A-65. Ref.6

Secondary structure

... 196
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform BimEL [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 531C176E5F1AC9AA

FASTA19622,067
        10         20         30         40         50         60 
MAKQPSDVSS ECDREGGQLQ PAERPPQLRP GAPTSLQTEP QGNPDGEGDR CPHGSPQGPL 

        70         80         90        100        110        120 
APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS PAPMSCDKST QTPSPPCQAF 

       130        140        150        160        170        180 
NHYLSAMASI RQSQEEPEDL RPEIRIAQEL RRIGDEFNET YTRRVFANDY REAEDHPQMV 

       190 
ILQLLRFIFR LVWRRH 

« Hide

Isoform BimL [UniParc].

Checksum: B127B40A190DA923
Show »

FASTA14016,205
Isoform BimS [UniParc].

Checksum: 67AB421AC1E4D2CA
Show »

FASTA11012,954

References

« Hide 'large scale' references
[1]"Bim: a novel member of the Bcl-2 family that promotes apoptosis."
O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S.
EMBO J. 17:384-395(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BIMEL).
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1."
Maurer U., Charvet C., Wagman A.S., Dejardin E., Green D.R.
Mol. Cell 21:749-760(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MCL1.
[5]"Neuronal apoptosis induced by endoplasmic reticulum stress is regulated by ATF4-CHOP-mediated induction of the Bcl-2 homology 3-only member PUMA."
Galehdar Z., Swan P., Fuerth B., Callaghan S.M., Park D.S., Cregan S.P.
J. Neurosci. 30:16938-16948(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYNLL1; TRIM2 AND YWHAZ, UBIQUITINATION, PHOSPHORYLATION AT SER-65, MUTAGENESIS OF SER-55; SER-65 AND SER-73.
[7]"The structure of a Bcl-xL/Bim fragment complex: implications for Bim function."
Liu X., Dai S., Zhu Y., Marrack P., Kappler J.W.
Immunity 19:341-352(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 139-171 IN COMPLEX WITH BCL2L1 ISOFORM BCL-XL, IDENTIFICATION IN A COMPLEX WITH DYNLL1 AND BCL-XL, INTERACTION WITH BAX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF032459 mRNA. Translation: AAC40029.1.
AF032460 mRNA. Translation: AAC40030.1.
AF032461 mRNA. Translation: AAC40031.1.
AL805950 Genomic DNA. Translation: CAM14745.1.
BC019556 mRNA. Translation: AAH19556.1.
CCDSCCDS16712.1. [O54918-3]
CCDS16713.1. [O54918-1]
CCDS16714.1. [O54918-2]
RefSeqNP_033884.1. NM_009754.3. [O54918-3]
NP_997563.1. NM_207680.2. [O54918-1]
NP_997564.1. NM_207681.2. [O54918-2]
UniGeneMm.141083.
Mm.453214.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PQ1X-ray1.65B139-171[»]
DisProtDP00518.
ProteinModelPortalO54918.
SMRO54918. Positions 139-171.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198352. 12 interactions.
DIPDIP-29214N.
IntActO54918. 16 interactions.
MINTMINT-89451.

PTM databases

PhosphoSiteO54918.

Proteomic databases

PaxDbO54918.
PRIDEO54918.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019281; ENSMUSP00000019281; ENSMUSG00000027381. [O54918-3]
ENSMUST00000103210; ENSMUSP00000099499; ENSMUSG00000027381. [O54918-2]
ENSMUST00000103211; ENSMUSP00000099500; ENSMUSG00000027381. [O54918-3]
ENSMUST00000110341; ENSMUSP00000105970; ENSMUSG00000027381. [O54918-1]
GeneID12125.
KEGGmmu:12125.
UCSCuc008mgh.1. mouse. [O54918-1]
uc008mgk.1. mouse. [O54918-2]

Organism-specific databases

CTD10018.
MGIMGI:1197519. Bcl2l11.

Phylogenomic databases

eggNOGNOG40262.
GeneTreeENSGT00390000003178.
HOGENOMHOG000231637.
InParanoidO54918.
KOK16341.
OMAIRLVWRM.
PhylomeDBO54918.
TreeFamTF335898.

Gene expression databases

ArrayExpressO54918.
BgeeO54918.
CleanExMM_BCL2L11.
GenevestigatorO54918.

Family and domain databases

InterProIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFPIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO54918.
NextBio280451.
PROO54918.
SOURCESearch...

Entry information

Entry nameB2L11_MOUSE
AccessionPrimary (citable) accession number: O54918
Secondary accession number(s): A2ALQ6, O54919, O54920
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot