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Protein

Transcription factor E2F6

Gene

E2f6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi50 – 129Sequence analysisAdd BLAST80

GO - Molecular functioni

  • DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F6
Short name:
E2F-6
Alternative name(s):
E2F-binding site-modulating activity protein
Short name:
EMA
Gene namesi
Name:E2f6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1354159. E2f6.

Subcellular locationi

GO - Cellular componenti

  • DNA replication factor A complex Source: MGI
  • MLL1 complex Source: UniProtKB
  • nucleus Source: MGI
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194731 – 272Transcription factor E2F6Add BLAST272

Proteomic databases

PaxDbiO54917.
PeptideAtlasiO54917.
PRIDEiO54917.

PTM databases

iPTMnetiO54917.
PhosphoSitePlusiO54917.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057469.
CleanExiMM_E2F6.
GenevisibleiO54917. MM.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10 (By similarity).By similarity

Protein-protein interaction databases

BioGridi206042. 4 interactors.
DIPiDIP-59331N.
STRINGi10090.ENSMUSP00000020908.

Structurei

3D structure databases

ProteinModelPortaliO54917.
SMRiO54917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 62Binding to corepressorsCuratedAdd BLAST62
Regioni130 – 222DimerizationSequence analysisAdd BLAST93
Regioni143 – 164Leucine-zipperAdd BLAST22
Regioni173 – 272Transcription repressionBy similarityAdd BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 129DEF boxAdd BLAST35

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000112309.
HOVERGENiHBG002227.
InParanoidiO54917.
KOiK09390.
OMAiSESTHAE.
OrthoDBiEOG091G087U.
TreeFamiTF105566.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR015635. Transcription_factor_E2F6.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF19. PTHR12081:SF19. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

O54917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQRTARRQ PSLLVDPAQE TVRRRCRDPI NVENLLPSKI RINLEENVQY
60 70 80 90 100
VSMRKALKVK RPRFDVSLVY LTRKFMDLVR SAPGGILDLN KVATKLGVRK
110 120 130 140 150
RRVYDITNVL DGIELVEKKS KNHIRWIGSD LNNFGAAPQQ KKLQAELSDL
160 170 180 190 200
SAMEDALDEL IKDCAQQLLE LTDDKENERL AYVTYQDIHG IQAFHEQIVI
210 220 230 240 250
AVKAPEETRL DVPAPREDSI TVHIRSTKGP IDVYLCEVEQ NHSNGKTNDG
260 270
IGASPSKSSH PQCPEKEDEP PQ
Length:272
Mass (Da):30,852
Last modified:October 3, 2012 - v2
Checksum:iB99641329C00CD08
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97G → S in AAC53521 (PubMed:9403682).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032131 mRNA. Translation: AAC53521.1.
AF487711 Genomic DNA. Translation: AAM45941.1.
AC157352 Genomic DNA. No translation available.
CCDSiCCDS36409.1.
RefSeqiNP_150373.2. NM_033270.2.
UniGeneiMm.23296.

Genome annotation databases

EnsembliENSMUST00000020908; ENSMUSP00000020908; ENSMUSG00000057469.
GeneIDi50496.
KEGGimmu:50496.
UCSCiuc007nce.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032131 mRNA. Translation: AAC53521.1.
AF487711 Genomic DNA. Translation: AAM45941.1.
AC157352 Genomic DNA. No translation available.
CCDSiCCDS36409.1.
RefSeqiNP_150373.2. NM_033270.2.
UniGeneiMm.23296.

3D structure databases

ProteinModelPortaliO54917.
SMRiO54917.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206042. 4 interactors.
DIPiDIP-59331N.
STRINGi10090.ENSMUSP00000020908.

PTM databases

iPTMnetiO54917.
PhosphoSitePlusiO54917.

Proteomic databases

PaxDbiO54917.
PeptideAtlasiO54917.
PRIDEiO54917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020908; ENSMUSP00000020908; ENSMUSG00000057469.
GeneIDi50496.
KEGGimmu:50496.
UCSCiuc007nce.2. mouse.

Organism-specific databases

CTDi1876.
MGIiMGI:1354159. E2f6.

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000112309.
HOVERGENiHBG002227.
InParanoidiO54917.
KOiK09390.
OMAiSESTHAE.
OrthoDBiEOG091G087U.
TreeFamiTF105566.

Miscellaneous databases

ChiTaRSiE2f6. mouse.
PROiO54917.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057469.
CleanExiMM_E2F6.
GenevisibleiO54917. MM.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR015635. Transcription_factor_E2F6.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF19. PTHR12081:SF19. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE2F6_MOUSE
AccessioniPrimary (citable) accession number: O54917
Secondary accession number(s): Q8K456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.