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Protein

RalBP1-associated Eps domain-containing protein 1

Gene

Reps1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi331 – 34212PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RalBP1-associated Eps domain-containing protein 1
Alternative name(s):
RalBP1-interacting protein 1
Gene namesi
Name:Reps1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1196373. Reps1.

Subcellular locationi

  • Membraneclathrin-coated pit By similarity

  • Note: Colocalize with ITSN1 at the plasma membrane in structures that are most probably clathrin-coated pits.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795RalBP1-associated Eps domain-containing protein 1PRO_0000073830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei145 – 1451PhosphoserineBy similarity
Modified residuei162 – 1621PhosphoserineBy similarity
Modified residuei166 – 1661PhosphoserineBy similarity
Modified residuei170 – 1701PhosphoserineBy similarity
Modified residuei173 – 1731PhosphothreonineBy similarity
Modified residuei272 – 2721PhosphoserineCombined sources
Modified residuei273 – 2731PhosphoserineCombined sources
Modified residuei288 – 2881PhosphotyrosineSequence analysis
Modified residuei307 – 3071PhosphoserineBy similarity
Modified residuei489 – 4891PhosphoserineBy similarity
Modified residuei539 – 5391PhosphoserineCombined sources
Modified residuei543 – 5431PhosphothreonineCombined sources
Modified residuei561 – 5611PhosphoserineBy similarity
Modified residuei708 – 7081PhosphoserineCombined sources
Modified residuei739 – 7391PhosphoserineBy similarity

Post-translational modificationi

EGF stimulates phosphorylation on Tyr-residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO54916.
MaxQBiO54916.
PaxDbiO54916.
PRIDEiO54916.

PTM databases

iPTMnetiO54916.
PhosphoSiteiO54916.

Expressioni

Tissue specificityi

Expressed in all tissues examined. The highest level expression was found in the kidney and testis.

Gene expression databases

BgeeiO54916.
CleanExiMM_REPS1.
ExpressionAtlasiO54916. baseline and differential.
GenevisibleiO54916. MM.

Interactioni

Subunit structurei

Homodimer (Potential). Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase. Interacts with RAB11FIP2 (By similarity). Interacts with AMPH, ITSN1 (via SH3 domains) and SGIP1; may be involved in clathrin-mediated endocytosis (By similarity).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

IntActiO54916. 4 interactions.
MINTiMINT-1592094.
STRINGi10090.ENSMUSP00000123238.

Structurei

Secondary structure

1
795
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi283 – 29311Combined sources
Turni294 – 2963Combined sources
Beta strandi303 – 3053Combined sources
Helixi306 – 31611Combined sources
Helixi320 – 33011Combined sources
Beta strandi335 – 3395Combined sources
Helixi340 – 35516Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FI6NMR-A279-370[»]
ProteinModelPortaliO54916.
SMRiO54916. Positions 245-370.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 113104EH 1PROSITE-ProRule annotationAdd
BLAST
Domaini285 – 37490EH 2PROSITE-ProRule annotationAdd
BLAST
Domaini318 – 35336EF-handPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni651 – 795145Interaction with RALBP1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili750 – 79041Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi540 – 60364Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 2 EH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IRAU. Eukaryota.
ENOG410ZSYS. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000231382.
HOVERGENiHBG056372.
InParanoidiO54916.
KOiK20068.
OMAiSHDPVQP.
PhylomeDBiO54916.
TreeFamiTF316546.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
[Graphical view]
PfamiPF12763. EF-hand_4. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54916-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGLTLSDAE QKYYSDLFSY CDIESTKKVV VNGRVLELFR AAQLPNDVVL
60 70 80 90 100
QIMELCGATR LGYFGRSQFY IALKLVAVAQ SGFPLRVESI NTVKDLPLPR
110 120 130 140 150
FVASKNEQES RLAASYSSDS ENQGSYSGVI PPPPGRGQVK KGPGSHDAVQ
160 170 180 190 200
PRPSAEQQEP VSPVVSPQQS PPTSPHTWRK HSRHPSGGNS ERPLTGPGPF
210 220 230 240 250
WSPFGDAQAG SSAGDAVWSG QSPPPPQDNW VSFADTPPTS ALLTMHPASV
260 270 280 290 300
QDQTTVRTVA SAATANEIRR QSSSYEDPWK ITDEQRQYYV NQFKTIQPDL
310 320 330 340 350
NGFIPGSAAK EFFTKSKLPI LELSHIWELS DFDKDGALTL DEFCAAFHLV
360 370 380 390 400
VARKNGYDLP EKLPESLMPK LIDLEDSADV GEQPGEVGYS GSPAEAPPSK
410 420 430 440 450
SPSMPSLNQT WPELNQSSEQ WETFSERSSS SQTLTQFDSN IAPADPDTAI
460 470 480 490 500
VHPVPIRMTP SKIHMQEMEL KRTSSDHTNP TSPLLVKPSD LSEENKINSS
510 520 530 540 550
VKFPSGNTVD GYSSSDSFPS DPEQIGSSVT RQRSHSGTSP DNTAPPPPPP
560 570 580 590 600
RPQPSHSRSS SLDMNRTFAV TTGQQQAGVV AHPPAVPPRP QPSQAPGPSV
610 620 630 640 650
HRPVDADGLI THTSTSPQQI PEQPNFADFS QFEVFAASNV SEEQDSEAEK
660 670 680 690 700
HPEVLPAEKA SDPSSSLRAA QADSKAEEKT ATNVPANVSK GTTPLAPPPK
710 720 730 740 750
PVRRRLKSED ELRPDVDEHT QKTGVLAAVL TSQPSIPRSV GKDKKAIQAS
760 770 780 790
IRRNKETNTV LARLNSELQQ QLKDVLEERI SLEVQLEQLR PFSHL
Length:795
Mass (Da):86,519
Last modified:November 3, 2009 - v2
Checksum:i98D788160F560509
GO
Isoform 2 (identifier: O54916-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
     420-454: QWETFSERSSSSQTLTQFDSNIAPADPDTAIVHPV → VSKTSLSLLEISLFTGRSFKQDRFTAGYLQYAHTP
     455-795: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:210
Mass (Da):23,378
Checksum:i0F50314C618EC731
GO
Isoform 3 (identifier: O54916-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     420-446: Missing.

Show »
Length:716
Mass (Da):77,614
Checksum:i2D37A40F959AA466
GO
Isoform 4 (identifier: O54916-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:743
Mass (Da):80,626
Checksum:i205B87DD9D8AC3A6
GO

Sequence cautioni

The sequence BAE29439.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE30291.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611V → A in AAB94736 (PubMed:9395447).Curated
Sequence conflicti605 – 6051D → N in BAE29439 (PubMed:16141072).Curated
Sequence conflicti701 – 7011P → T in BAE29439 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 244244Missing in isoform 2. 1 PublicationVSP_038337Add
BLAST
Alternative sequencei1 – 5252Missing in isoform 3 and isoform 4. 2 PublicationsVSP_038336Add
BLAST
Alternative sequencei420 – 45435QWETF…IVHPV → VSKTSLSLLEISLFTGRSFK QDRFTAGYLQYAHTP in isoform 2. 1 PublicationVSP_007956Add
BLAST
Alternative sequencei420 – 44627Missing in isoform 3. 1 PublicationVSP_038338Add
BLAST
Alternative sequencei455 – 795341Missing in isoform 2. 1 PublicationVSP_007957Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031939 mRNA. Translation: AAB94736.1.
AK150284 mRNA. Translation: BAE29439.1. Different initiation.
AK151309 mRNA. Translation: BAE30291.1. Different initiation.
AK041967 mRNA. Translation: BAC31117.1.
AC153433 Genomic DNA. No translation available.
BC002256 mRNA. No translation available.
BC087547 mRNA. Translation: AAH87547.1.
CCDSiCCDS23710.2. [O54916-1]
PIRiT09173.
RefSeqiNP_001104535.1. NM_001111065.1.
NP_033074.2. NM_009048.2. [O54916-1]
UniGeneiMm.4479.

Genome annotation databases

EnsembliENSMUST00000126390; ENSMUSP00000123238; ENSMUSG00000019854. [O54916-1]
GeneIDi19707.
KEGGimmu:19707.
UCSCiuc007ema.2. mouse. [O54916-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031939 mRNA. Translation: AAB94736.1.
AK150284 mRNA. Translation: BAE29439.1. Different initiation.
AK151309 mRNA. Translation: BAE30291.1. Different initiation.
AK041967 mRNA. Translation: BAC31117.1.
AC153433 Genomic DNA. No translation available.
BC002256 mRNA. No translation available.
BC087547 mRNA. Translation: AAH87547.1.
CCDSiCCDS23710.2. [O54916-1]
PIRiT09173.
RefSeqiNP_001104535.1. NM_001111065.1.
NP_033074.2. NM_009048.2. [O54916-1]
UniGeneiMm.4479.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FI6NMR-A279-370[»]
ProteinModelPortaliO54916.
SMRiO54916. Positions 245-370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54916. 4 interactions.
MINTiMINT-1592094.
STRINGi10090.ENSMUSP00000123238.

PTM databases

iPTMnetiO54916.
PhosphoSiteiO54916.

Proteomic databases

EPDiO54916.
MaxQBiO54916.
PaxDbiO54916.
PRIDEiO54916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000126390; ENSMUSP00000123238; ENSMUSG00000019854. [O54916-1]
GeneIDi19707.
KEGGimmu:19707.
UCSCiuc007ema.2. mouse. [O54916-1]

Organism-specific databases

CTDi85021.
MGIiMGI:1196373. Reps1.

Phylogenomic databases

eggNOGiENOG410IRAU. Eukaryota.
ENOG410ZSYS. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000231382.
HOVERGENiHBG056372.
InParanoidiO54916.
KOiK20068.
OMAiSHDPVQP.
PhylomeDBiO54916.
TreeFamiTF316546.

Miscellaneous databases

ChiTaRSiReps1. mouse.
EvolutionaryTraceiO54916.
PROiO54916.
SOURCEiSearch...

Gene expression databases

BgeeiO54916.
CleanExiMM_REPS1.
ExpressionAtlasiO54916. baseline and differential.
GenevisibleiO54916. MM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
[Graphical view]
PfamiPF12763. EF-hand_4. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An eps homology (EH) domain protein that binds to the ral-GTPase target, RalBP1."
    Yamaguchi A., Urano T., Goi T., Feig L.A.
    J. Biol. Chem. 272:31230-31234(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), CHARACTERIZATION.
    Tissue: Muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-795 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 499-795 (ISOFORM 1).
    Tissue: Embryo and Mammary tumor.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-708, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272; SER-273; SER-539 AND THR-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  8. "Solution structure of the Reps1 EH domain and characterization of its binding to NPF target sequences."
    Kim S., Cullis D.N., Feig L.A., Baleja J.D.
    Biochemistry 40:6776-6785(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 279-370.

Entry informationi

Entry nameiREPS1_MOUSE
AccessioniPrimary (citable) accession number: O54916
Secondary accession number(s): Q3UAM3
, Q5PPQ9, Q8C9J9, Q99LR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: November 3, 2009
Last modified: June 8, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.