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O54915

- NR1I2_MOUSE

UniProt

O54915 - NR1I2_MOUSE

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Protein

Nuclear receptor subfamily 1 group I member 2

Gene

Nr1i2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes (By similarity). Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei244 – 2441AgonistBy similarity
Binding sitei285 – 2851AgonistBy similarity
Binding sitei296 – 2961AgonistBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi35 – 10470Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri38 – 5821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri74 – 9926NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB
  3. RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl
  4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  5. steroid hormone receptor activity Source: InterPro
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. drug export Source: UniProtKB
  2. exogenous drug catabolic process Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: MGI
  4. positive regulation of gene expression Source: MGI
  5. positive regulation of transcription, DNA-templated Source: UniProtKB
  6. xenobiotic metabolic process Source: UniProtKB
  7. xenobiotic transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_234105. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 1 group I member 2
Alternative name(s):
Orphan nuclear receptor PXR
Pregnane X receptor
Gene namesi
Name:Nr1i2
Synonyms:Pxr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1337040. Nr1i2.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Nuclear receptor subfamily 1 group I member 2PRO_0000053549Add
BLAST

Proteomic databases

PRIDEiO54915.

PTM databases

PhosphoSiteiO54915.

Expressioni

Gene expression databases

BgeeiO54915.
CleanExiMM_NR1I2.
ExpressionAtlasiO54915. baseline and differential.
GenevestigatoriO54915.

Interactioni

Subunit structurei

Heterodimer with RXRA. Interacts with NCOA1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201839. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliO54915.
SMRiO54915. Positions 34-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 20197HingeAdd
BLAST
Regioni202 – 431230Ligand-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 8927Bipartite nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 5821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri74 – 9926NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG238483.
HOGENOMiHOG000220844.
HOVERGENiHBG108655.
InParanoidiO54915.
KOiK08540.
OMAiHFKNFRL.
OrthoDBiEOG7GBFXG.
PhylomeDBiO54915.
TreeFamiTF316304.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O54915-1) [UniParc]FASTAAdd to Basket

Also known as: PXR.1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPEESWSRV GLVQCEEADS ALEEPINVEE EDGGLQICRV CGDKANGYHF
60 70 80 90 100
NVMTCEGCKG FFRRAMKRNV RLRCPFRKGT CEITRKTRRQ CQACRLRKCL
110 120 130 140 150
ESGMKKEMIM SDAAVEQRRA LIKRKKREKI EAPPPGGQGL TEEQQALIQE
160 170 180 190 200
LMDAQMQTFD TTFSHFKDFR LPAVFHSGCE LPEFLQASLL EDPATWSQIM
210 220 230 240 250
KDRVPMKISL QLRGEDGSIW NYQPPSKSDG KEIIPLLPHL ADVSTYMFKG
260 270 280 290 300
VINFAKVISY FRDLPIEDQI SLLKGATFEM CILRFNTMFD TETGTWECGR
310 320 330 340 350
LAYCFEDPNG GFQKLLLDPL MKFHCMLKKL QLHKEEYVLM QAISLFSPDR
360 370 380 390 400
PGVVQRSVVD QLQERFALTL KAYIECSRPY PAHRFLFLKI MAVLTELRSI
410 420 430
NAQQTQQLLR IQDSHPFATP LMQELFSSTD G
Length:431
Mass (Da):49,567
Last modified:June 1, 1998 - v1
Checksum:iF592AF91F689329E
GO
Isoform 2 (identifier: O54915-2) [UniParc]FASTAAdd to Basket

Also known as: PXR.2

The sequence of this isoform differs from the canonical sequence as follows:
     171-211: Missing.

Show »
Length:390
Mass (Da):44,958
Checksum:i9425A40F980F08ED
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 21141Missing in isoform 2. CuratedVSP_003670Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031814 mRNA. Translation: AAC39964.1.
AK018630 mRNA. Translation: BAB31316.1.
CCDSiCCDS28164.1. [O54915-1]
RefSeqiNP_001091874.1. NM_001098404.1. [O54915-2]
NP_035066.1. NM_010936.3. [O54915-1]
XP_006521909.1. XM_006521846.1. [O54915-1]
XP_006521911.1. XM_006521848.1. [O54915-1]
UniGeneiMm.8509.

Genome annotation databases

EnsembliENSMUST00000023504; ENSMUSP00000023504; ENSMUSG00000022809. [O54915-1]
GeneIDi18171.
KEGGimmu:18171.
UCSCiuc007zep.1. mouse. [O54915-2]
uc007zeq.1. mouse. [O54915-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031814 mRNA. Translation: AAC39964.1 .
AK018630 mRNA. Translation: BAB31316.1 .
CCDSi CCDS28164.1. [O54915-1 ]
RefSeqi NP_001091874.1. NM_001098404.1. [O54915-2 ]
NP_035066.1. NM_010936.3. [O54915-1 ]
XP_006521909.1. XM_006521846.1. [O54915-1 ]
XP_006521911.1. XM_006521848.1. [O54915-1 ]
UniGenei Mm.8509.

3D structure databases

ProteinModelPortali O54915.
SMRi O54915. Positions 34-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201839. 5 interactions.

Chemistry

ChEMBLi CHEMBL1743244.
GuidetoPHARMACOLOGYi 606.

PTM databases

PhosphoSitei O54915.

Proteomic databases

PRIDEi O54915.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023504 ; ENSMUSP00000023504 ; ENSMUSG00000022809 . [O54915-1 ]
GeneIDi 18171.
KEGGi mmu:18171.
UCSCi uc007zep.1. mouse. [O54915-2 ]
uc007zeq.1. mouse. [O54915-1 ]

Organism-specific databases

CTDi 8856.
MGIi MGI:1337040. Nr1i2.

Phylogenomic databases

eggNOGi NOG238483.
HOGENOMi HOG000220844.
HOVERGENi HBG108655.
InParanoidi O54915.
KOi K08540.
OMAi HFKNFRL.
OrthoDBi EOG7GBFXG.
PhylomeDBi O54915.
TreeFami TF316304.

Enzyme and pathway databases

Reactomei REACT_234105. Nuclear Receptor transcription pathway.

Miscellaneous databases

NextBioi 293468.
PROi O54915.
SOURCEi Search...

Gene expression databases

Bgeei O54915.
CleanExi MM_NR1I2.
ExpressionAtlasi O54915. baseline and differential.
Genevestigatori O54915.

Family and domain databases

Gene3Di 1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway."
    Kliewer S.A., Moore J.T., Wade L., Staudinger J.L., Watson M.A., Jones S.A., McKee D.D., Oliver B.B., Willson T.M., Zetterstrom R.H., Perlmann T., Lehmann J.M.
    Cell 92:73-82(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cecum.
  3. "Molecular mechanism of nuclear translocation of an orphan nuclear receptor, SXR."
    Kawana K., Ikuta T., Kobayashi Y., Gotoh O., Takeda K., Kawajiri K.
    Mol. Pharmacol. 63:524-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Identification of an endogenous ligand that activates pregnane X receptor-mediated sterol clearance."
    Dussault I., Yoo H.-D., Lin M., Wang E., Fan M., Batta A.K., Salen G., Erickson S.K., Forman B.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:833-838(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Human CYP3A4 and murine Cyp3A11 are regulated by equol and genistein via the pregnane X receptor in a species-specific manner."
    Li Y., Ross-Viola J.S., Shay N.F., Moore D.D., Ricketts M.L.
    J. Nutr. 139:898-904(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNR1I2_MOUSE
AccessioniPrimary (citable) accession number: O54915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3