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O54915

- NR1I2_MOUSE

UniProt

O54915 - NR1I2_MOUSE

Protein

Nuclear receptor subfamily 1 group I member 2

Gene

Nr1i2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes By similarity. Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).By similarity2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei244 – 2441AgonistBy similarity
    Binding sitei285 – 2851AgonistBy similarity
    Binding sitei296 – 2961AgonistBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi35 – 10470Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri38 – 5821NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri74 – 9926NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. drug binding Source: UniProtKB
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB
    3. protein binding Source: MGI
    4. sequence-specific DNA binding Source: InterPro
    5. steroid hormone receptor activity Source: InterPro
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. drug export Source: UniProtKB
    2. exogenous drug catabolic process Source: UniProtKB
    3. negative regulation of transcription, DNA-templated Source: MGI
    4. positive regulation of gene expression Source: MGI
    5. positive regulation of transcription, DNA-templated Source: UniProtKB
    6. xenobiotic metabolic process Source: UniProtKB
    7. xenobiotic transport Source: UniProtKB

    Keywords - Molecular functioni

    Activator, Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 1 group I member 2
    Alternative name(s):
    Orphan nuclear receptor PXR
    Pregnane X receptor
    Gene namesi
    Name:Nr1i2
    Synonyms:Pxr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1337040. Nr1i2.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 431431Nuclear receptor subfamily 1 group I member 2PRO_0000053549Add
    BLAST

    Proteomic databases

    PRIDEiO54915.

    PTM databases

    PhosphoSiteiO54915.

    Expressioni

    Gene expression databases

    ArrayExpressiO54915.
    BgeeiO54915.
    CleanExiMM_NR1I2.
    GenevestigatoriO54915.

    Interactioni

    Subunit structurei

    Heterodimer with RXRA. Interacts with NCOA1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201839. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO54915.
    SMRiO54915. Positions 34-426.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni105 – 20197HingeAdd
    BLAST
    Regioni202 – 431230Ligand-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi63 – 8927Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri38 – 5821NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri74 – 9926NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG238483.
    HOGENOMiHOG000220844.
    HOVERGENiHBG108655.
    InParanoidiO54915.
    KOiK08540.
    OMAiHFKNFRL.
    OrthoDBiEOG7GBFXG.
    PhylomeDBiO54915.
    TreeFamiTF316304.

    Family and domain databases

    Gene3Di1.10.565.10. 2 hits.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O54915-1) [UniParc]FASTAAdd to Basket

    Also known as: PXR.1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPEESWSRV GLVQCEEADS ALEEPINVEE EDGGLQICRV CGDKANGYHF    50
    NVMTCEGCKG FFRRAMKRNV RLRCPFRKGT CEITRKTRRQ CQACRLRKCL 100
    ESGMKKEMIM SDAAVEQRRA LIKRKKREKI EAPPPGGQGL TEEQQALIQE 150
    LMDAQMQTFD TTFSHFKDFR LPAVFHSGCE LPEFLQASLL EDPATWSQIM 200
    KDRVPMKISL QLRGEDGSIW NYQPPSKSDG KEIIPLLPHL ADVSTYMFKG 250
    VINFAKVISY FRDLPIEDQI SLLKGATFEM CILRFNTMFD TETGTWECGR 300
    LAYCFEDPNG GFQKLLLDPL MKFHCMLKKL QLHKEEYVLM QAISLFSPDR 350
    PGVVQRSVVD QLQERFALTL KAYIECSRPY PAHRFLFLKI MAVLTELRSI 400
    NAQQTQQLLR IQDSHPFATP LMQELFSSTD G 431
    Length:431
    Mass (Da):49,567
    Last modified:June 1, 1998 - v1
    Checksum:iF592AF91F689329E
    GO
    Isoform 2 (identifier: O54915-2) [UniParc]FASTAAdd to Basket

    Also known as: PXR.2

    The sequence of this isoform differs from the canonical sequence as follows:
         171-211: Missing.

    Show »
    Length:390
    Mass (Da):44,958
    Checksum:i9425A40F980F08ED
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei171 – 21141Missing in isoform 2. CuratedVSP_003670Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031814 mRNA. Translation: AAC39964.1.
    AK018630 mRNA. Translation: BAB31316.1.
    CCDSiCCDS28164.1. [O54915-1]
    RefSeqiNP_001091874.1. NM_001098404.1. [O54915-2]
    NP_035066.1. NM_010936.3. [O54915-1]
    XP_006521909.1. XM_006521846.1. [O54915-1]
    XP_006521911.1. XM_006521848.1. [O54915-1]
    UniGeneiMm.8509.

    Genome annotation databases

    EnsembliENSMUST00000023504; ENSMUSP00000023504; ENSMUSG00000022809. [O54915-1]
    GeneIDi18171.
    KEGGimmu:18171.
    UCSCiuc007zep.1. mouse. [O54915-2]
    uc007zeq.1. mouse. [O54915-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031814 mRNA. Translation: AAC39964.1 .
    AK018630 mRNA. Translation: BAB31316.1 .
    CCDSi CCDS28164.1. [O54915-1 ]
    RefSeqi NP_001091874.1. NM_001098404.1. [O54915-2 ]
    NP_035066.1. NM_010936.3. [O54915-1 ]
    XP_006521909.1. XM_006521846.1. [O54915-1 ]
    XP_006521911.1. XM_006521848.1. [O54915-1 ]
    UniGenei Mm.8509.

    3D structure databases

    ProteinModelPortali O54915.
    SMRi O54915. Positions 34-426.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201839. 5 interactions.

    Chemistry

    ChEMBLi CHEMBL1743244.
    GuidetoPHARMACOLOGYi 606.

    PTM databases

    PhosphoSitei O54915.

    Proteomic databases

    PRIDEi O54915.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023504 ; ENSMUSP00000023504 ; ENSMUSG00000022809 . [O54915-1 ]
    GeneIDi 18171.
    KEGGi mmu:18171.
    UCSCi uc007zep.1. mouse. [O54915-2 ]
    uc007zeq.1. mouse. [O54915-1 ]

    Organism-specific databases

    CTDi 8856.
    MGIi MGI:1337040. Nr1i2.

    Phylogenomic databases

    eggNOGi NOG238483.
    HOGENOMi HOG000220844.
    HOVERGENi HBG108655.
    InParanoidi O54915.
    KOi K08540.
    OMAi HFKNFRL.
    OrthoDBi EOG7GBFXG.
    PhylomeDBi O54915.
    TreeFami TF316304.

    Miscellaneous databases

    NextBioi 293468.
    PROi O54915.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54915.
    Bgeei O54915.
    CleanExi MM_NR1I2.
    Genevestigatori O54915.

    Family and domain databases

    Gene3Di 1.10.565.10. 2 hits.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway."
      Kliewer S.A., Moore J.T., Wade L., Staudinger J.L., Watson M.A., Jones S.A., McKee D.D., Oliver B.B., Willson T.M., Zetterstrom R.H., Perlmann T., Lehmann J.M.
      Cell 92:73-82(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Cecum.
    3. "Molecular mechanism of nuclear translocation of an orphan nuclear receptor, SXR."
      Kawana K., Ikuta T., Kobayashi Y., Gotoh O., Takeda K., Kawajiri K.
      Mol. Pharmacol. 63:524-531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Identification of an endogenous ligand that activates pregnane X receptor-mediated sterol clearance."
      Dussault I., Yoo H.-D., Lin M., Wang E., Fan M., Batta A.K., Salen G., Erickson S.K., Forman B.M.
      Proc. Natl. Acad. Sci. U.S.A. 100:833-838(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Human CYP3A4 and murine Cyp3A11 are regulated by equol and genistein via the pregnane X receptor in a species-specific manner."
      Li Y., Ross-Viola J.S., Shay N.F., Moore D.D., Ricketts M.L.
      J. Nutr. 139:898-904(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNR1I2_MOUSE
    AccessioniPrimary (citable) accession number: O54915
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3