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Protein

NF-kappa-B inhibitor epsilon

Gene

Nfkbie

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_275686. Activation of NF-kappaB in B cells.

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor epsilon
Short name:
NF-kappa-BIE
Alternative name(s):
I-kappa-B-epsilon
Short name:
IkB-E
Short name:
IkB-epsilon
Short name:
IkappaBepsilon
Gene namesi
Name:Nfkbie
Synonyms:Ikbe
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1194908. Nfkbie.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364NF-kappa-B inhibitor epsilonPRO_0000067008Add
BLAST

Post-translational modificationi

Serine phosphorylated; followed by proteasome-dependent degradation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54910.
PaxDbiO54910.
PRIDEiO54910.

PTM databases

PhosphoSiteiO54910.

Expressioni

Gene expression databases

BgeeiO54910.
GenevisibleiO54910. MM.

Interactioni

Subunit structurei

Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RelbQ048632EBI-6688774,EBI-1209145

Protein-protein interaction databases

IntActiO54910. 1 interaction.
STRINGi10090.ENSMUSP00000024742.

Structurei

3D structure databases

ProteinModelPortaliO54910.
SMRiO54910. Positions 33-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati122 – 15534ANK 1Add
BLAST
Repeati157 – 18630ANK 2Add
BLAST
Repeati190 – 21930ANK 3Add
BLAST
Repeati233 – 26230ANK 4Add
BLAST
Repeati267 – 29630ANK 5Add
BLAST
Repeati300 – 32930ANK 6Add
BLAST

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000059576.
HOVERGENiHBG108320.
InParanoidiO54910.
KOiK05872.
OMAiRNRPLME.
OrthoDBiEOG7W154S.
PhylomeDBiO54910.
TreeFamiTF320166.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDARKGPDE ADDSQCDSGI ESLRSLRSLP EPTAAPGSGS SQSGCPQPWR
60 70 80 90 100
HAPETHKEPE KEDADGERAD STYASSSLTE SFPLLERPEA KDPSPPAPGS
110 120 130 140 150
PLPPAGVLSP QQLEALTYIS EDGDTLLHLA VIHEAPSVLF CCLAFLPQEV
160 170 180 190 200
LDIQNNLYQT ALHLAVHLDQ PDVVRALVLK GASRILQDQH GDTALHVACR
210 220 230 240 250
RQNLACACCL LEEQPEPGRQ LSHPLDLQLK NWQGLACLHI ATLQRNQPLI
260 270 280 290 300
ELLLQNGADI DVQEGTSGKT ALHLAVETQE RSLVQFLLQA GARVDARMLN
310 320 330 340 350
GCTPLHLAAG RGLNSISSTL CEAGADSLLL NVEDETPQDL AEDLLSYLPF
360
DDLKISGKPL LCTD
Length:364
Mass (Da):39,200
Last modified:June 20, 2001 - v2
Checksum:i4426B58424187F3A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971A → V in AAB97517 (PubMed:9405619).Curated
Sequence conflicti182 – 19110ASRILQDQHG → PAEFCRSAC in BAB25924 (PubMed:16141072).Curated
Sequence conflicti291 – 2911G → R in AAB97517 (PubMed:9405619).Curated
Sequence conflicti345 – 36420LSYLP…LLCTD → GWVPTASHLEAGSAAAAVGT QAMSPLC in BAB27943 (PubMed:16141072).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030896 mRNA. Translation: AAB97517.1.
AK008843 mRNA. Translation: BAB25924.1.
AK011965 mRNA. Translation: BAB27943.1.
AK151450 mRNA. Translation: BAE30410.1.
AK153248 mRNA. Translation: BAE31839.1.
BC030923 mRNA. Translation: AAH30923.1.
CCDSiCCDS28811.1.
RefSeqiNP_001291885.1. NM_001304956.1.
NP_032716.2. NM_008690.4.
UniGeneiMm.57043.

Genome annotation databases

EnsembliENSMUST00000024742; ENSMUSP00000024742; ENSMUSG00000023947.
GeneIDi18037.
KEGGimmu:18037.
UCSCiuc008cqv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030896 mRNA. Translation: AAB97517.1.
AK008843 mRNA. Translation: BAB25924.1.
AK011965 mRNA. Translation: BAB27943.1.
AK151450 mRNA. Translation: BAE30410.1.
AK153248 mRNA. Translation: BAE31839.1.
BC030923 mRNA. Translation: AAH30923.1.
CCDSiCCDS28811.1.
RefSeqiNP_001291885.1. NM_001304956.1.
NP_032716.2. NM_008690.4.
UniGeneiMm.57043.

3D structure databases

ProteinModelPortaliO54910.
SMRiO54910. Positions 33-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54910. 1 interaction.
STRINGi10090.ENSMUSP00000024742.

PTM databases

PhosphoSiteiO54910.

Proteomic databases

MaxQBiO54910.
PaxDbiO54910.
PRIDEiO54910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024742; ENSMUSP00000024742; ENSMUSG00000023947.
GeneIDi18037.
KEGGimmu:18037.
UCSCiuc008cqv.2. mouse.

Organism-specific databases

CTDi4794.
MGIiMGI:1194908. Nfkbie.

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000059576.
HOVERGENiHBG108320.
InParanoidiO54910.
KOiK05872.
OMAiRNRPLME.
OrthoDBiEOG7W154S.
PhylomeDBiO54910.
TreeFamiTF320166.

Enzyme and pathway databases

ReactomeiREACT_275686. Activation of NF-kappaB in B cells.

Miscellaneous databases

NextBioi293137.
PROiO54910.
SOURCEiSearch...

Gene expression databases

BgeeiO54910.
GenevisibleiO54910. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterization of mouse IkappaBepsilon."
    Simeonidis S., Liang S., Chen G., Thanos D.
    Proc. Natl. Acad. Sci. U.S.A. 94:14372-14377(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow, Embryo and Stomach.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiIKBE_MOUSE
AccessioniPrimary (citable) accession number: O54910
Secondary accession number(s): Q3U686, Q9CZZ9, Q9D7U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: July 22, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.