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Protein

Tumor necrosis factor ligand superfamily member 12

Gene

Tnfsf12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein

Keywords - Biological processi

Angiogenesis, Apoptosis, Differentiation

Enzyme and pathway databases

ReactomeiR-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor necrosis factor ligand superfamily member 12
Alternative name(s):
TNF-related weak inducer of apoptosis
Short name:
TWEAK
Cleaved into the following 2 chains:
Gene namesi
Name:Tnfsf12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1196259. Tnfsf12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4524Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini46 – 249204ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Tumor necrosis factor ligand superfamily member 12, membrane formPRO_0000034522Add
BLAST
Chaini94 – 249156Tumor necrosis factor ligand superfamily member 12, secreted formBy similarityPRO_0000034523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi191 ↔ 210By similarity

Post-translational modificationi

The soluble form is produced from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei93 – 942CleavageBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO54907.
PRIDEiO54907.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

ExpressionAtlasiO54907. baseline and differential.
GenevisibleiO54907. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with the angiogenic factor AGGF1/VG5Q (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137658.

Structurei

3D structure databases

ProteinModelPortaliO54907.
SMRiO54907. Positions 105-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II60. Eukaryota.
ENOG410Y4KC. LUCA.
HOVERGENiHBG055510.
InParanoidiO54907.
KOiK05474.
OMAiNPLRYDC.
OrthoDBiEOG79KPGF.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARRSQRRR GRRGEPGTAL LAPLVLSLGL ALACLGLLLV VVSLGSWATL
60 70 80 90 100
SAQEPSQEEL TAEDRREPPE LNPQTEESQD VVPFLEQLVR PRRSAPKGRK
110 120 130 140 150
ARPRRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEETKINS SSPLRYDRQI
160 170 180 190 200
GEFTVIRAGL YYLYCQVHFD EGKAVYLKLD LLVNGVLALR CLEEFSATAA
210 220 230 240
SSPGPQLRLC QVSGLLPLRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH
Length:249
Mass (Da):27,441
Last modified:May 24, 2004 - v2
Checksum:i08352620444EFB7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030100 mRNA. Translation: AAC53517.2.
AK020909 mRNA. Translation: BAB32249.1.
CCDSiCCDS24907.1.
RefSeqiNP_035744.1. NM_011614.3.
UniGeneiMm.8983.

Genome annotation databases

EnsembliENSMUST00000181810; ENSMUSP00000137972; ENSMUSG00000097328.
GeneIDi21944.
KEGGimmu:21944.
UCSCiuc007jri.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030100 mRNA. Translation: AAC53517.2.
AK020909 mRNA. Translation: BAB32249.1.
CCDSiCCDS24907.1.
RefSeqiNP_035744.1. NM_011614.3.
UniGeneiMm.8983.

3D structure databases

ProteinModelPortaliO54907.
SMRiO54907. Positions 105-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137658.

Proteomic databases

PaxDbiO54907.
PRIDEiO54907.

Protocols and materials databases

DNASUi21944.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000181810; ENSMUSP00000137972; ENSMUSG00000097328.
GeneIDi21944.
KEGGimmu:21944.
UCSCiuc007jri.2. mouse.

Organism-specific databases

CTDi8742.
MGIiMGI:1196259. Tnfsf12.

Phylogenomic databases

eggNOGiENOG410II60. Eukaryota.
ENOG410Y4KC. LUCA.
HOVERGENiHBG055510.
InParanoidiO54907.
KOiK05474.
OMAiNPLRYDC.
OrthoDBiEOG79KPGF.

Enzyme and pathway databases

ReactomeiR-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Miscellaneous databases

PROiO54907.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiO54907. baseline and differential.
GenevisibleiO54907. MM.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Chicheportiche Y., Bixler S., Tizard R., Browning J.L.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis."
    Chicheportiche Y., Bourdon P.R., Xu H., Hsu Y.-M., Scott H., Hession C., Garcia I., Browning J.L.
    J. Biol. Chem. 272:32401-32410(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-249.
    Tissue: Peritoneal macrophage.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 107-249.
    Strain: C57BL/6J.
    Tissue: Retina.

Entry informationi

Entry nameiTNF12_MOUSE
AccessioniPrimary (citable) accession number: O54907
Secondary accession number(s): Q9CTP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 24, 2004
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.