O54905 (B3GT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 2
Short name=Beta-1,3-GalTase 2
Short name=Beta3Gal-T2
Short name=Beta3GalT2
EC=2.4.1.-

Alternative name(s):
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase-II

Gene names

Name:

B3galt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Can also utilize substrates with a terminal galactose residue, albeit with lower efficiency. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Ref.1
Cofactor	Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Detected in brain and heart. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oligosaccharide biosynthetic process Inferred from direct assay Ref.1. Source: MGI protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Beta-1,3-galactosyltransferase 2		PRO_0000219151
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 43	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	44 – 422	379	Lumenal Potential
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	226	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	44	1	N → S in strain: HMI/Msf. Ref.4
	Natural variant	86	1	V → A in strain: BLG2/Msf, MSM/Msf, SWN/Msf and NJL/Msf. Ref.4
	Natural variant	88	1	P → S in strain: BLG2/Msf and NJL/Msf. Ref.4
	Natural variant	100	1	S → T in strain: CAST/Ei and HMI/Msf. Ref.4
	Natural variant	124	1	N → D in strain: BLG2/Msf, NJL/Msf, MSM/Msf and SWN/Msf. Ref.4
Experimental info
	Sequence conflict	95	1	T → I in AAC53524. Ref.1
	Sequence conflict	366	1	H → R in BAC28688. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O54905 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 58F5D3568143F4FB	FASTA	422	49,095
	10 20 30 40 50 60 MLQWRRRHCC FAKMTWSPKR SLLRTPLTGV LSLVFLFAMF LFFNHHDWLP GRPGFKENPV 70 80 90 100 110 120 TYTFRGFRST KSETNHSSLR TIWKEVAPQT LRPHTASNSS NTELSPQGVT GLQNTLSANG 130 140 150 160 170 180 SIYNEKGTGH PNSYHFKYII NEPEKCQEKS PFLILLIAAE PGQIEARRAI RQTWGNETLA 190 200 210 220 230 240 PGIQIIRVFL LGISIKLNGY LQHAIQEESR QYHDIIQQEY LDTYYNLTIK TLMGMNWVAT 250 260 270 280 290 300 YCPHTPYVMK TDSDMFVNTE YLIHKLLKPD LPPRHNYFTG YLMRGYAPNR NKDSKWYMPP 310 320 330 340 350 360 DLYPSERYPV FCSGTGYVFS GDLAEKIFKV SLGIRRLHLE DVYVGICLAK LRVDPVPPPN 370 380 390 400 410 420 EFVFNHWRVS YSSCKYSHLI TSHQFQPSEL IKYWNHLQQN KHNACANAAK EKAGRYRHRK LH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes." Hennet T., Dinter A., Kuhnert P., Mattu T.S., Rudd P.M., Berger E.G. J. Biol. Chem. 273:58-65(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, TISSUE SPECIFICITY. Strain: 129/SvJ.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum, Diencephalon and Hippocampus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[4]	"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies." Liu Y., Kitano T., Koide T., Shiroishi T., Moriwaki K., Saitou N. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-415, VARIANTS SER-44; ALA-86; SER-88; THR-100 AND ASP-124. Strain: BFM/2Msf, BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, Pgn2 and SWN/Msf.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b3GalT2

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029791 Genomic DNA. Translation: AAC53524.1. AK034371 mRNA. Translation: BAC28688.1. AK036141 mRNA. Translation: BAC29317.1. AK083168 mRNA. Translation: BAC38793.1. BC046322 mRNA. Translation: AAH46322.1. AB039144 Genomic DNA. Translation: BAB68668.1. AB039145 Genomic DNA. Translation: BAB68669.1. AB039146 Genomic DNA. Translation: BAB68670.1. AB039147 Genomic DNA. Translation: BAB68671.1. AB039148 Genomic DNA. Translation: BAB68672.1. AB039149 Genomic DNA. Translation: BAB68673.1. AB039150 Genomic DNA. Translation: BAB68674.1. AB039151 Genomic DNA. Translation: BAB68675.1. AB039152 Genomic DNA. Translation: BAB68676.1.
IPI	IPI00387260.
RefSeq	NP_064409.3. NM_020025.4.
UniGene	Mm.285580.
3D structure databases
ProteinModelPortal	O54905.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
PTM databases
PhosphoSite	O54905.
Proteomic databases
PRIDE	O54905.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038252; ENSMUSP00000046118; ENSMUSG00000033849.
GeneID	26878.
KEGG	mmu:26878.
UCSC	uc007cwy.2. mouse.
Organism-specific databases
CTD	8707.
MGI	MGI:1349461. B3galt2.
Phylogenomic databases
eggNOG	NOG331548.
GeneTree	ENSGT00670000097716.
HOVERGEN	HBG101354.
InParanoid	O54905.
KO	K07820.
OMA	CCFAKMS.
OrthoDB	EOG4FXR7D.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	O54905.
Genevestigator	O54905.
GermOnline	ENSMUSG00000033849. Mus musculus.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. PTHR11214. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304691.
SOURCE	Search...

Entry information

Entry name

B3GT2_MOUSE

Accession

Primary (citable) accession number: O54905
Secondary accession number(s): Q8BH19 , Q8CBX4, Q91V19, Q91V58, Q91VE9, Q920V3, Q920V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program