O54904 (B3GT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 1
Short name=Beta-1,3-GalTase 1
Short name=Beta3Gal-T1
Short name=Beta3GalT1
EC=2.4.1.-

Alternative name(s):
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase-I
UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1

Gene names

Name:

B3galt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Ref.1
Cofactor	Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oligosaccharide biosynthetic process Inferred from direct assay Ref.1. Source: MGI protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from direct assay PubMed 11042173. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Beta-1,3-galactosyltransferase 1		PRO_0000219146
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 26	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	27 – 326	300	Lumenal Potential
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	12	1	T → S in strain: 129/SvJ. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54904 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 83271E99B2EE74F5	FASTA	326	37,993
	10 20 30 40 50 60 MASKVSCLYV LTVVCWASAL WYLSITRPTS SYTGSKPFSH LTVARKNFTF GNIRTRPINP 70 80 90 100 110 120 HSFEFLINEP NKCEKNIPFL VILISTTHKE FDARQAIRET WGDENNFKGI KIATLFLLGK 130 140 150 160 170 180 NADPVLNQMV EQESQIFHDI IVEDFIDSYH NLTLKTLMGM RWVATFCSKA KYVMKTDSDI 190 200 210 220 230 240 FVNMDNLIYK LLKPSTKPRR RYFTGYVING GPIRDVRSKW YMPRDLYPDS NYPPFCSGTG 250 260 270 280 290 300 YIFSADVAEL IYKTSLHTRL LHLEDVYVGL CLRKLGIHPF QNSGFNHWKM AYSLCRYRRV 310 320 ITVHQISPEE MHRIWNDMSS KKHLRC

References

[1]	"Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes." Hennet T., Dinter A., Kuhnert P., Mattu T.S., Rudd P.M., Berger E.G. J. Biol. Chem. 273:58-65(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-12, FUNCTION, COFACTOR, TISSUE SPECIFICITY. Strain: 129/SvJ.
[2]	"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies." Liu Y., Kitano T., Koide T., Shiroishi T., Moriwaki K., Saitou N. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-316. Strain: BFM/2Msf, BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, Pgn2 and SWN/Msf.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b3GalT1

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029790 Genomic DNA. Translation: AAC53523.1. AB039134 Genomic DNA. Translation: BAB68658.1. AB039135 Genomic DNA. Translation: BAB68659.1. AB039136 Genomic DNA. Translation: BAB68660.1. AB039137 Genomic DNA. Translation: BAB68661.1. AB039138 Genomic DNA. Translation: BAB68662.1. AB039139 Genomic DNA. Translation: BAB68663.1. AB039140 Genomic DNA. Translation: BAB68664.1. AB039141 Genomic DNA. Translation: BAB68665.1. AB039142 Genomic DNA. Translation: BAB68666.1.
IPI	IPI00119757.
RefSeq	NP_064679.2. NM_020283.4.
UniGene	Mm.226435. Mm.490783.
3D structure databases
ProteinModelPortal	O54904.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
PTM databases
PhosphoSite	O54904.
Proteomic databases
PRIDE	O54904.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000042456; ENSMUSP00000041343; ENSMUSG00000034780. ENSMUST00000112346; ENSMUSP00000107965; ENSMUSG00000034780.
GeneID	26877.
KEGG	mmu:26877.
Organism-specific databases
CTD	8708.
MGI	MGI:1349403. B3galt1.
Phylogenomic databases
eggNOG	NOG271304.
HOGENOM	HOG000059604.
HOVERGEN	HBG101354.
InParanoid	O54904.
KO	K07819.
OMA	DFLINEP.
OrthoDB	EOG4DJJWT.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	O54904.
Bgee	O54904.
Genevestigator	O54904.
GermOnline	ENSMUSG00000034780. Mus musculus.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. PTHR11214. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304687.
SOURCE	Search...

Entry information

Entry name

B3GT1_MOUSE

Accession

Primary (citable) accession number: O54904
Secondary accession number(s): Q91V52

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program