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O54890

- ITB3_MOUSE

UniProt

O54890 - ITB3_MOUSE

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Protein

Integrin beta-3

Gene
Itgb3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

GO - Molecular functioni

  1. integrin binding Source: MGI
  2. protein binding Source: UniProtKB
  3. receptor activity Source: InterPro

GO - Biological processi

  1. angiogenesis involved in wound healing Source: InterPro
  2. cell-matrix adhesion Source: MGI
  3. cell projection morphogenesis Source: BHF-UCL
  4. cell-substrate junction assembly Source: MGI
  5. integrin-mediated signaling pathway Source: UniProtKB-KW
  6. platelet aggregation Source: InterPro
  7. positive regulation of bone resorption Source: BHF-UCL
  8. positive regulation of cell migration Source: BHF-UCL
  9. positive regulation of osteoblast proliferation Source: BHF-UCL
  10. positive regulation of osteoclast differentiation Source: BHF-UCL
  11. regulation of cell migration Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_196606. ECM proteoglycans.
REACT_196644. Syndecan interactions.
REACT_198996. Elastic fibre formation.
REACT_198998. Molecules associated with elastic fibres.
REACT_215461. Signal transduction by L1.
REACT_216309. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-3
Alternative name(s):
Platelet membrane glycoprotein IIIa
Short name:
GPIIIa
CD_antigen: CD61
Gene namesi
Name:Itgb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:96612. Itgb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 717692Extracellular Reviewed predictionAdd
BLAST
Transmembranei718 – 74023Helical; Reviewed predictionAdd
BLAST
Topological domaini741 – 78747Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. alpha9-beta1 integrin-ADAM8 complex Source: BHF-UCL
  2. apical plasma membrane Source: MGI
  3. external side of plasma membrane Source: MGI
  4. integrin alpha9-beta1 complex Source: BHF-UCL
  5. nucleus Source: MGI
  6. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Chaini26 – 787762Integrin beta-3PRO_0000016345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 460 By similarity
Disulfide bondi38 ↔ 48 By similarity
Disulfide bondi41 ↔ 74 By similarity
Disulfide bondi51 ↔ 63 By similarity
Disulfide bondi202 ↔ 209 By similarity
Disulfide bondi257 ↔ 298 By similarity
Glycosylationi345 – 3451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi396 – 3961N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi399 ↔ 411 By similarity
Disulfide bondi431 ↔ 680 By similarity
Disulfide bondi458 ↔ 462 By similarity
Disulfide bondi473 ↔ 485 By similarity
Glycosylationi477 – 4771N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi482 ↔ 520 By similarity
Disulfide bondi487 ↔ 496 By similarity
Disulfide bondi498 ↔ 511 By similarity
Disulfide bondi526 ↔ 531 By similarity
Disulfide bondi528 ↔ 561 By similarity
Disulfide bondi533 ↔ 546 By similarity
Disulfide bondi548 ↔ 553 By similarity
Disulfide bondi567 ↔ 572 By similarity
Disulfide bondi569 ↔ 600 By similarity
Disulfide bondi574 ↔ 583 By similarity
Glycosylationi584 – 5841N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi585 ↔ 592 By similarity
Disulfide bondi606 ↔ 611 By similarity
Disulfide bondi608 ↔ 656 By similarity
Disulfide bondi613 ↔ 623 By similarity
Disulfide bondi626 ↔ 629 By similarity
Disulfide bondi633 ↔ 642 By similarity
Disulfide bondi639 ↔ 712 By similarity
Disulfide bondi660 ↔ 688 By similarity
Glycosylationi679 – 6791N-linked (GlcNAc...) Reviewed prediction
Modified residuei772 – 7721Phosphotyrosine By similarity
Modified residuei778 – 7781Phosphothreonine By similarity
Modified residuei784 – 7841Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO54890.
PaxDbiO54890.
PRIDEiO54890.

PTM databases

PhosphoSiteiO54890.

Expressioni

Gene expression databases

BgeeiO54890.
CleanExiMM_ITGB3.
GenevestigatoriO54890.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIB or alpha-V. Interacts with FLNB and COMP. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion. Interacts with MYO10 By similarity. Interacts with DAB2. Interacts with FERMT2.2 Publications

Protein-protein interaction databases

BioGridi200830. 3 interactions.
DIPiDIP-46416N.
IntActiO54890. 4 interactions.
MINTiMINT-4099028.
STRINGi10090.ENSMUSP00000021028.

Structurei

3D structure databases

ProteinModelPortaliO54890.
SMRiO54890. Positions 28-787.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 376243VWFAAdd
BLAST
Repeati462 – 51049IAdd
BLAST
Repeati511 – 55242IIAdd
BLAST
Repeati553 – 59139IIIAdd
BLAST
Repeati592 – 62837IVAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni462 – 628167Cysteine-rich tandem repeatsAdd
BLAST

Sequence similaritiesi

Contains 1 VWFA domain.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287997.
GeneTreeiENSGT00720000108389.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiQ3TZC6.
KOiK06493.
OMAiGHGQCSC.
OrthoDBiEOG7T7GSB.
TreeFamiTF105392.

Family and domain databases

Gene3Di1.20.5.630. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR027068. Integrin_beta-3.
IPR015812. Integrin_bsu.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF25. PTHR10082:SF25. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54890-1 [UniParc]FASTAAdd to Basket

« Hide

MRAQWPGQLW AALLALGALA GVVVGESNIC TTRGVNSCQQ CLAVSPVCAW    50
CSDETLSQGS PRCNLKENLL KDNCAPESIE FPVSEAQILE ARPLSSKGSG 100
SSAQITQVSP QRIALRLRPD DSKIFSLQVR QVEDYPVDIY YLMDLSFSMK 150
DDLSSIQTLG TKLASQMRKL TSNLRIGFGA FVDKPVSPYM YISPPQAIKN 200
PCYNMKNACL PMFGYKHVLT LTDQVSRFNE EVKKQSVSRN RDAPEGGFDA 250
IMQATVCDEK IGWRNDASHL LVFTTDAKTH IALDGRLAGI VLPNDGHCHI 300
GTDNHYSAST TMDYPSLGLM TEKLSQKNIN LIFAVTENVV SLYQNYSELI 350
PGTTVGVLSD DSSNVLQLIV DAYGKIRSKV ELEVRDLPEE LSLSFNATCL 400
NNEVIPGLKS CVGLKIGDTV SFSIEAKVRG CPQEKEQSFT IKPVGFKDSL 450
TVQVTFDCDC ACQAFAQPSS PRCNNGNGTF ECGVCRCDQG WLGSMCECSE 500
EDYRPSQQEE CSPKEGQPIC SQRGECLCGQ CVCHSSDFGK ITGKYCECDD 550
FSCVRYKGEM CSGHGQCNCG DCVCDSDWTG YYCNCTTRTD TCMSTNGLLC 600
SGRGNCECGS CVCVQPGSYG DTCEKCPTCP DACSFKKECV ECKKFNRGTL 650
HEENTCSRYC RDDIEQVKEL TDTGKNAVNC TYKNEDDCVV RFQYYEDTSG 700
RAVLYVVEEP ECPKGPDILV VLLSVMGAIL LIGLATLLIW KLLITIHDRK 750
EFAKFEEERA RAKWDTANNP LYKEATSTFT NITYRGT 787
Length:787
Mass (Da):86,738
Last modified:July 27, 2011 - v2
Checksum:i4F2E606969788794
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141A → V in AAB94086. 1 Publication
Sequence conflicti389 – 3891E → G in AAB94086. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF026509 mRNA. Translation: AAB94086.1.
AK157958 mRNA. Translation: BAE34283.1.
AL603709, BX000996 Genomic DNA. Translation: CAM22629.1.
BX000996, AL603709 Genomic DNA. Translation: CAM27926.1.
CH466558 Genomic DNA. Translation: EDL34227.1.
BC125518 mRNA. Translation: AAI25519.1.
BC125520 mRNA. Translation: AAI25521.1.
CCDSiCCDS25536.1.
PIRiPN0510.
RefSeqiNP_058060.2. NM_016780.2.
UniGeneiMm.87150.

Genome annotation databases

EnsembliENSMUST00000021028; ENSMUSP00000021028; ENSMUSG00000020689.
GeneIDi16416.
KEGGimmu:16416.
UCSCiuc007lwx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF026509 mRNA. Translation: AAB94086.1 .
AK157958 mRNA. Translation: BAE34283.1 .
AL603709 , BX000996 Genomic DNA. Translation: CAM22629.1 .
BX000996 , AL603709 Genomic DNA. Translation: CAM27926.1 .
CH466558 Genomic DNA. Translation: EDL34227.1 .
BC125518 mRNA. Translation: AAI25519.1 .
BC125520 mRNA. Translation: AAI25521.1 .
CCDSi CCDS25536.1.
PIRi PN0510.
RefSeqi NP_058060.2. NM_016780.2.
UniGenei Mm.87150.

3D structure databases

ProteinModelPortali O54890.
SMRi O54890. Positions 28-787.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200830. 3 interactions.
DIPi DIP-46416N.
IntActi O54890. 4 interactions.
MINTi MINT-4099028.
STRINGi 10090.ENSMUSP00000021028.

Chemistry

BindingDBi O54890.

PTM databases

PhosphoSitei O54890.

Proteomic databases

MaxQBi O54890.
PaxDbi O54890.
PRIDEi O54890.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021028 ; ENSMUSP00000021028 ; ENSMUSG00000020689 .
GeneIDi 16416.
KEGGi mmu:16416.
UCSCi uc007lwx.2. mouse.

Organism-specific databases

CTDi 3690.
MGIi MGI:96612. Itgb3.

Phylogenomic databases

eggNOGi NOG287997.
GeneTreei ENSGT00720000108389.
HOGENOMi HOG000252936.
HOVERGENi HBG006190.
InParanoidi Q3TZC6.
KOi K06493.
OMAi GHGQCSC.
OrthoDBi EOG7T7GSB.
TreeFami TF105392.

Enzyme and pathway databases

Reactomei REACT_196606. ECM proteoglycans.
REACT_196644. Syndecan interactions.
REACT_198996. Elastic fibre formation.
REACT_198998. Molecules associated with elastic fibres.
REACT_215461. Signal transduction by L1.
REACT_216309. Integrin cell surface interactions.

Miscellaneous databases

ChiTaRSi ITGB3. mouse.
NextBioi 289613.
PROi O54890.
SOURCEi Search...

Gene expression databases

Bgeei O54890.
CleanExi MM_ITGB3.
Genevestigatori O54890.

Family and domain databases

Gene3Di 1.20.5.630. 1 hit.
3.40.50.410. 1 hit.
InterProi IPR027068. Integrin_beta-3.
IPR015812. Integrin_bsu.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view ]
PANTHERi PTHR10082. PTHR10082. 1 hit.
PTHR10082:SF25. PTHR10082:SF25. 1 hit.
Pfami PF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view ]
PIRSFi PIRSF002512. Integrin_B. 1 hit.
PRINTSi PR01186. INTEGRINB.
SMARTi SM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEi PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 1 hit.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. McHugh K.P., Teitelbaum S.L., Kitazawa S., Ross F.P.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HeN.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Inner ear.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling."
    Calderwood D.A., Fujioka Y., de Pereda J.M., Garcia-Alvarez B., Nakamoto T., Margolis B., McGlade C.J., Liddington R.C., Ginsberg M.H.
    Proc. Natl. Acad. Sci. U.S.A. 100:2272-2277(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DAB2.
  7. "Kindlin-2 controls bidirectional signaling of integrins."
    Montanez E., Ussar S., Schifferer M., Bosl M., Zent R., Moser M., Fassler R.
    Genes Dev. 22:1325-1330(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FERMT2.

Entry informationi

Entry nameiITB3_MOUSE
AccessioniPrimary (citable) accession number: O54890
Secondary accession number(s): Q3TZC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi