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Protein

DNA-directed RNA polymerase I subunit RPA1

Gene

Polr1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641ZincBy similarity
Metal bindingi67 – 671ZincBy similarity
Metal bindingi74 – 741ZincBy similarity
Metal bindingi77 – 771ZincBy similarity
Metal bindingi595 – 5951Magnesium; catalyticBy similarity
Metal bindingi597 – 5971Magnesium; catalyticBy similarity
Metal bindingi599 – 5991Magnesium; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • rRNA transcription Source: RGD
  • transcription from RNA polymerase I promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA1 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit A1
Alternative name(s):
DNA-directed RNA polymerase I largest subunit
DNA-directed RNA polymerase I subunit A
RNA polymerase I 194 kDa subunit
Short name:
A194
Short name:
RPA194
Gene namesi
Name:Polr1a
Synonyms:Rpa1, Rpo1-4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620824. Polr1a.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17161716DNA-directed RNA polymerase I subunit RPA1PRO_0000073925Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54889.
PRIDEiO54889.

PTM databases

PhosphoSiteiO54889.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with MYO1C. Interacts with ERBB2 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1507847.
STRINGi10116.ENSRNOP00000013587.

Structurei

3D structure databases

ProteinModelPortaliO54889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni968 – 98013Bridging helixBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
HOGENOMiHOG000205401.
HOVERGENiHBG017741.
InParanoidiO54889.
KOiK02999.
PhylomeDBiO54889.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASKHTPWR RLQGISFGMY SAEELKKLSV KSITNPRYVD SLGNPSADGL
60 70 80 90 100
YDLALGPADS KEVCSTCVQD FNNCSGHLGH IDLPLTVYNP LLFDKLYLLL
110 120 130 140 150
RGSCLNCHML TCPRAAIHLL VCQLKVLDVG ALQAVYELER ILSRFLEETS
160 170 180 190 200
DPSAFEIQEE LEEYTSKILQ NNLLGSQGAH VKNVCESRSK LVAHFWKTHM
210 220 230 240 250
AAKRCPHCKT GRSVVRKEHN SKLTITYPAM VHKKSGQKDA ELPEGAPAAP
260 270 280 290 300
GIDEAQMGKR GYLTPSSAQE HLFAIWKNEG FFLNYLFSGL DDIGPESSFN
310 320 330 340 350
PSMFFLDFIV VPPSRYRPIN RLGDQMFTNG QTVNLQAVMK DAVLIRKLLA
360 370 380 390 400
VMAQEQKLPC EMTEITIDKE NDSSGAIDRS FLSLLPGQSL TDKLYNIWIR
410 420 430 440 450
LQSHVNIVFD SDMDKLMLEK YPGIRQILEK KEGLFRKHMM GKRVDYAARS
460 470 480 490 500
VICPDMYINT NEIGIPMVFA TKLTYPQPVT PWNVQELRQA VINGPNVHPG
510 520 530 540 550
ASMVINEDGS RTALSAVDAT QREAVAKQLL TPSTGIPKPQ GAKVVCRHVK
560 570 580 590 600
NGDILLLNRQ PTLHRPSIQA HRAHILPEEK VLRLHYANCK AYNADFDGDE
610 620 630 640 650
MNAHFPQSEL GRAEAYVLAC TDQQYLVPKD GQPLAGLIQD HMVSGANMTI
660 670 680 690 700
RGCFFTREQY MELVYRGLTD KVGRVKLFPP AILKPFPLWT GKQVVSTLLI
710 720 730 740 750
NIIPEDYTPL NLTGKAKIGS KAWVKEKPRP VPDFDPDSMC ESQVIIREGE
760 770 780 790 800
LLCGVLDKAH YGSSAYGLVH CCYEIYGGET SGRVLTCLAR LFTAYLQLYR
810 820 830 840 850
GFTLGVEDIL VKPNADVMRQ RIIEESTQCG PRAVRAALNL PEAASCDEIQ
860 870 880 890 900
GKWQDAIWRK DQRDFNMIDM KFKEEVNHYS NEINKACMPF GLHRQFPENN
910 920 930 940 950
LQMMVQSGAK GSTVNTMQIS CLLGQIELEG RRPPLMASGK SLPCFEPYEF
960 970 980 990 1000
TPRAGGFVTG RFLTGIRPPE FFFHCMAGRE GLVDTAVKTS RSGYLQRCII
1010 1020 1030 1040 1050
KHLEGLVIQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ PKQFPFLASN
1060 1070 1080 1090 1100
YEVIMKSKHL HEVLSRADPQ KVLRHFRAIK KWHHRHSSAL LRKGAFLSFS
1110 1120 1130 1140 1150
QKIQAAVKAL NLEGKTQNGR SPETQQMLQM WHELDEQSRR KYQKRAAPCP
1160 1170 1180 1190 1200
DPSLSVWRPD IHFASVSETF EKKIDDYSQE WAAQAEKSHN RSELSLDRLR
1210 1220 1230 1240 1250
TLLQLKWQRS LCDPGEAVGL LAAQSIGEPS TQMTLNTFHF AGRGEMNVTL
1260 1270 1280 1290 1300
GIPRLREILM VASANIKTPM MSVPVFNTKK ALRRVKSLKK QLTRVCLGEV
1310 1320 1330 1340 1350
LQKVDIQESF CMGEKQNKFR VYELRFQFLP HAYYQQEKCL RPEDILHFME
1360 1370 1380 1390 1400
TRFFKLLMEA IKKKNSKASA FRSVNTRRAT QKDLDDTEDS GRNRREEERD
1410 1420 1430 1440 1450
EEEEGNIVDA EAEEGDADAS DTKRKEKQEE EVDYESEEEG EEEEEEDVQE
1460 1470 1480 1490 1500
EENIKGEGAH QTHEPDEEEG SGLEEESSQN PPCRHSRPQG AEAMERRIQA
1510 1520 1530 1540 1550
VRESHSFIED YQYDTEESLW CQVTVKLPLM KINFDMSSLV VSLAHNAIVY
1560 1570 1580 1590 1600
TTKGITRCLL NETINSKNEK EFVLNTEGIN LPELFKYSEV LDLRRLYSND
1610 1620 1630 1640 1650
IHAVANTYGI EAALRVIEKE IKDVFAVYGI AVDPRHLSLV ADYMCFEGVY
1660 1670 1680 1690 1700
KPLNRFGIQS SSSPLQQMTF ETSFQFLKQA TMMGSHDELK SPSACLVVGK
1710
VVKGGTGLFE LKQPLR
Length:1,716
Mass (Da):194,192
Last modified:June 1, 1998 - v1
Checksum:iE8EE15BC23E60941
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025425 mRNA. Translation: AAB94601.1.
PIRiT14103.
RefSeqiNP_113960.1. NM_031772.1.
UniGeneiRn.24877.

Genome annotation databases

GeneIDi83581.
KEGGirno:83581.
UCSCiRGD:620824. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025425 mRNA. Translation: AAB94601.1.
PIRiT14103.
RefSeqiNP_113960.1. NM_031772.1.
UniGeneiRn.24877.

3D structure databases

ProteinModelPortaliO54889.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1507847.
STRINGi10116.ENSRNOP00000013587.

PTM databases

PhosphoSiteiO54889.

Proteomic databases

PaxDbiO54889.
PRIDEiO54889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83581.
KEGGirno:83581.
UCSCiRGD:620824. rat.

Organism-specific databases

CTDi25885.
RGDi620824. Polr1a.

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
HOGENOMiHOG000205401.
HOVERGENiHBG017741.
InParanoidiO54889.
KOiK02999.
PhylomeDBiO54889.

Miscellaneous databases

PROiO54889.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Affinity purification of mammalian RNA polymerase I. Identification of an associated kinase."
    Hannan R.D., Hempel W.M., Cavanaugh A., Arino T., Dimitrov S.I., Moss T., Rothblum L.
    J. Biol. Chem. 273:1257-1267(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE RNA POL I COMPLEX, PHOSPHORYLATION.

Entry informationi

Entry nameiRPA1_RAT
AccessioniPrimary (citable) accession number: O54889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.