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Protein

DNA-directed RNA polymerase I subunit RPA2

Gene

Polr1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1070 – 110132C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • rRNA transcription Source: RGD
  • transcription from RNA polymerase I promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA2 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit 2
Alternative name(s):
A127
RPA135
Gene namesi
Name:Polr1b
Synonyms:Rpa2, Rpo1-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620822. Polr1b.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11351135DNA-directed RNA polymerase I subunit RPA2PRO_0000048074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1051 – 10511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54888.
PRIDEiO54888.

PTM databases

iPTMnetiO54888.
PhosphoSiteiO54888.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024882.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1070 – 110132C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0216. Eukaryota.
COG0085. LUCA.
HOGENOMiHOG000222963.
HOVERGENiHBG017742.
InParanoidiO54888.
KOiK03002.
PhylomeDBiO54888.

Family and domain databases

Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDSRWRNL PSGPSLKHLT DPSYAVPPEQ QKAALQDLTR AHVDSFNYAV
60 70 80 90 100
LEGLSHAVQA IPPFEFAFKD ERISLTIVDA AISPPAVPKG TICKELNIYP
110 120 130 140 150
AECRGRRSTY RGKLTADISW AVNGVPKGII KQFLGXVPIM VKSKLCNLYN
160 170 180 190 200
LPPQVLIEHH EEAEEMGGYF IINGIEKVIR MLIMPRRNFP IAMIRPKWKS
210 220 230 240 250
RGLGYTQFGV SIHCVREEHS AVNMNLHYVE NGTVMLNFIY RKELFFLPLG
260 270 280 290 300
FALKALVSFS DYQIFQELIK GKEEDSFFKN SVSQMLRIVM EEGCHTQKQV
310 320 330 340 350
LDYLGERFRV KLSLPDWYPN AEAAEFLFNQ CICIHLKSNT DKFYLLCLMT
360 370 380 390 400
RKLFALARGE CMEDNPDSLV NQEVLTPGQL FLMFLKEKME NWLLSIKIAL
410 420 430 440 450
DKRAQKTNVS INNENLMKIF SMGTELTRPF EYLLATGNLR SKTGLGFMQD
460 470 480 490 500
SGLCVVADKL NFIRYLSHFR CVHRGADFAK MRTTTVRKLL PESWGFLCPV
510 520 530 540 550
HTPDGAPCGL LNHLTAVCEV VTKFVYTASI PALLCGLGVT PVDAAPCRPY
560 570 580 590 600
SDCYPVLLDG VMVGWVDKEL APEVADTLRR FKVLRERRCS SLDGGGPDSH
610 620 630 640 650
DRKAKPVPRA VPLHHSLQAG EACAEPGAGQ RRARWNYGAA LHEHCHLRGR
660 670 680 690 700
GFWWSFHTPG ALPSQPAEVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL
710 720 730 740 750
LTYQDRSDNK LYRLQTPQSP LVRPCMYDHY DMDNYPIGTN AIVAVISYTG
760 770 780 790 800
YDMEDAMIVN KASWERGFAH GSVYKSEFID LSEKFKQGDD SLVFGVKPGD
810 820 830 840 850
PRVMQKLDND GLPFIGAKLE FGDPYYGYLN LNTGEGFVVY YKSKENCVVD
860 870 880 890 900
NIKVCSNDTG SGKFKCVCVT VRVPRNPTIG DKFASRHGQK GILSRLWPAE
910 920 930 940 950
DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF
960 970 980 990 1000
IFSEENSALE YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ
1010 1020 1030 1040 1050
RLRHMVSDKF QVRTTGARDK VTNQPIGGRN VQGGIRFGEM ERDALLAHGT
1060 1070 1080 1090 1100
SFLLHDRLFN CSDRSVAHVC VKCGSLLSPL LEKPPPSWSA MRNRKYNCTV
1110 1120 1130
CGRSDSIDTV SVPYVFRYFV AELAAMNIKV KLDVI
Length:1,135
Mass (Da):127,721
Last modified:June 1, 1998 - v1
Checksum:i0A46D3FE9079A3E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025424 mRNA. Translation: AAB94600.1.
PIRiT42368.
RefSeqiNP_113961.1. NM_031773.1.
UniGeneiRn.88734.

Genome annotation databases

GeneIDi83582.
KEGGirno:83582.
UCSCiRGD:620822. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025424 mRNA. Translation: AAB94600.1.
PIRiT42368.
RefSeqiNP_113961.1. NM_031773.1.
UniGeneiRn.88734.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024882.

PTM databases

iPTMnetiO54888.
PhosphoSiteiO54888.

Proteomic databases

PaxDbiO54888.
PRIDEiO54888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83582.
KEGGirno:83582.
UCSCiRGD:620822. rat.

Organism-specific databases

CTDi84172.
RGDi620822. Polr1b.

Phylogenomic databases

eggNOGiKOG0216. Eukaryota.
COG0085. LUCA.
HOGENOMiHOG000222963.
HOVERGENiHBG017742.
InParanoidiO54888.
KOiK03002.
PhylomeDBiO54888.

Miscellaneous databases

PROiO54888.

Family and domain databases

Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Affinity purification of mammalian RNA polymerase I. Identification of an associated kinase."
    Hannan R.D., Hempel W.M., Cavanaugh A., Arino T., Dimitrov S.I., Moss T., Rothblum L.
    J. Biol. Chem. 273:1257-1267(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE RNA POL I COMPLEX.

Entry informationi

Entry nameiRPA2_RAT
AccessioniPrimary (citable) accession number: O54888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.