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O54888 (RPA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase I subunit RPA2
Short name=RNA polymerase I subunit 2
EC=2.7.7.6
Alternative name(s):
A127
RPA135
Gene names
Name:Polr1b
Synonyms:Rpa2, Rpo1-2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1135 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.

Subcellular location

Nucleus › nucleolus By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11351135DNA-directed RNA polymerase I subunit RPA2
PRO_0000048074

Regions

Zinc finger1070 – 110132C4-type Potential

Sequences

Sequence LengthMass (Da)Tools
O54888 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 0A46D3FE9079A3E3

FASTA1,135127,721
        10         20         30         40         50         60 
MDVDSRWRNL PSGPSLKHLT DPSYAVPPEQ QKAALQDLTR AHVDSFNYAV LEGLSHAVQA 

        70         80         90        100        110        120 
IPPFEFAFKD ERISLTIVDA AISPPAVPKG TICKELNIYP AECRGRRSTY RGKLTADISW 

       130        140        150        160        170        180 
AVNGVPKGII KQFLGXVPIM VKSKLCNLYN LPPQVLIEHH EEAEEMGGYF IINGIEKVIR 

       190        200        210        220        230        240 
MLIMPRRNFP IAMIRPKWKS RGLGYTQFGV SIHCVREEHS AVNMNLHYVE NGTVMLNFIY 

       250        260        270        280        290        300 
RKELFFLPLG FALKALVSFS DYQIFQELIK GKEEDSFFKN SVSQMLRIVM EEGCHTQKQV 

       310        320        330        340        350        360 
LDYLGERFRV KLSLPDWYPN AEAAEFLFNQ CICIHLKSNT DKFYLLCLMT RKLFALARGE 

       370        380        390        400        410        420 
CMEDNPDSLV NQEVLTPGQL FLMFLKEKME NWLLSIKIAL DKRAQKTNVS INNENLMKIF 

       430        440        450        460        470        480 
SMGTELTRPF EYLLATGNLR SKTGLGFMQD SGLCVVADKL NFIRYLSHFR CVHRGADFAK 

       490        500        510        520        530        540 
MRTTTVRKLL PESWGFLCPV HTPDGAPCGL LNHLTAVCEV VTKFVYTASI PALLCGLGVT 

       550        560        570        580        590        600 
PVDAAPCRPY SDCYPVLLDG VMVGWVDKEL APEVADTLRR FKVLRERRCS SLDGGGPDSH 

       610        620        630        640        650        660 
DRKAKPVPRA VPLHHSLQAG EACAEPGAGQ RRARWNYGAA LHEHCHLRGR GFWWSFHTPG 

       670        680        690        700        710        720 
ALPSQPAEVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL LTYQDRSDNK LYRLQTPQSP 

       730        740        750        760        770        780 
LVRPCMYDHY DMDNYPIGTN AIVAVISYTG YDMEDAMIVN KASWERGFAH GSVYKSEFID 

       790        800        810        820        830        840 
LSEKFKQGDD SLVFGVKPGD PRVMQKLDND GLPFIGAKLE FGDPYYGYLN LNTGEGFVVY 

       850        860        870        880        890        900 
YKSKENCVVD NIKVCSNDTG SGKFKCVCVT VRVPRNPTIG DKFASRHGQK GILSRLWPAE 

       910        920        930        940        950        960 
DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF IFSEENSALE 

       970        980        990       1000       1010       1020 
YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ RLRHMVSDKF QVRTTGARDK 

      1030       1040       1050       1060       1070       1080 
VTNQPIGGRN VQGGIRFGEM ERDALLAHGT SFLLHDRLFN CSDRSVAHVC VKCGSLLSPL 

      1090       1100       1110       1120       1130 
LEKPPPSWSA MRNRKYNCTV CGRSDSIDTV SVPYVFRYFV AELAAMNIKV KLDVI

« Hide

References

[1]"Affinity purification of mammalian RNA polymerase I. Identification of an associated kinase."
Hannan R.D., Hempel W.M., Cavanaugh A., Arino T., Dimitrov S.I., Moss T., Rothblum L.
J. Biol. Chem. 273:1257-1267(1998) [PubMed: 9422795] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE RNA POL I COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF025424 mRNA. Translation: AAB94600.1.
IPIIPI00231082.
PIRT42368.
RefSeqNP_113961.1. NM_031773.1.
UniGeneRn.88734.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO54888.

PTM databases

PhosphoSiteO54888.

Proteomic databases

PRIDEO54888.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID83582.
KEGGrno:83582.
UCSCNM_031773. rat.

Organism-specific databases

CTD84172.
RGD620822. Polr1b.

Phylogenomic databases

eggNOGroNOG09372.
GeneTreeENSGT00550000074993.
HOVERGENHBG017742.
InParanoidO54888.
PhylomeDBO54888.

Gene expression databases

ArrayExpressO54888.
GenevestigatorO54888.
GermOnlineENSRNOG00000018349. Rattus norvegicus.

Family and domain databases

InterProIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
[Graphical view]
Gene3DG3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits.
KOK03002.
PANTHERPTHR20856. RNA_pol_I_sub2. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio616115.

Entry information

Entry nameRPA2_RAT
AccessionPrimary (citable) accession number: O54888
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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