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Protein

TYRO protein tyrosine kinase-binding protein

Gene

Tyrobp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Important for interaction with transmembrane receptorsBy similarity1

GO - Molecular functioni

GO - Biological processi

  • innate immune response Source: Reactome
  • integrin-mediated signaling pathway Source: UniProtKB
  • macrophage activation involved in immune response Source: UniProtKB
  • neutrophil activation involved in immune response Source: UniProtKB
  • regulation of osteoclast development Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2172127. DAP12 interactions.
R-MMU-2424491. DAP12 signaling.
R-MMU-391160. Signal regulatory protein (SIRP) family interactions.
R-MMU-416700. Other semaphorin interactions.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
TYRO protein tyrosine kinase-binding protein
Alternative name(s):
DNAX-activation protein 12
Killer-activating receptor-associated protein
Short name:
KAR-associated protein
Gene namesi
Name:Tyrobp
Synonyms:Dap12, Karap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277211. Tyrobp.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 42ExtracellularSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Topological domaini64 – 114CytoplasmicSequence analysisAdd BLAST51

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002260522 – 114TYRO protein tyrosine kinase-binding proteinAdd BLAST93

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37InterchainBy similarity
Modified residuei92PhosphotyrosineCombined sources1
Modified residuei103PhosphotyrosineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated.

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO54885.
PeptideAtlasiO54885.
PRIDEiO54885.

PTM databases

iPTMnetiO54885.
PhosphoSitePlusiO54885.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030579.
CleanExiMM_TYROBP.
ExpressionAtlasiO54885. baseline and differential.
GenevisibleiO54885. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with SIGLEC14 (By similarity). Interacts with CLECSF5. Interacts with CD300LB and CD300D. Interacts with CD300E. Interacts with KLRC2 and KIR2DS3 (By similarity). Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophils and macrophages integrin-mediated activation. Interacts (via transmembrane domain) with KLRK1 isoform 2 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface expression and induce NK cell-mediated cytotoxicity.By similarity9 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204396. 5 interactors.
DIPiDIP-29746N.
STRINGi10090.ENSMUSP00000032800.

Structurei

3D structure databases

ProteinModelPortaliO54885.
SMRiO54885.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 109ITAMAdd BLAST29

Sequence similaritiesi

Belongs to the TYROBP family.Curated
Contains 1 ITAM domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J1XS. Eukaryota.
ENOG410Z4TN. LUCA.
GeneTreeiENSGT00390000016786.
HOGENOMiHOG000056440.
HOVERGENiHBG061468.
InParanoidiO54885.
KOiK07992.
OrthoDBiEOG091G0X44.
PhylomeDBiO54885.
TreeFamiTF336898.

Family and domain databases

InterProiIPR026200. Tyrobp.
[Graphical view]
PANTHERiPTHR17554. PTHR17554. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGALEPSWCL LFLPVLLTVG GLSPVQAQSD TFPRCDCSSV SPGVLAGIVL
60 70 80 90 100
GDLVLTLLIA LAVYSLGRLV SRGQGTAEGT RKQHIAETES PYQELQGQRP
110
EVYSDLNTQR QYYR
Length:114
Mass (Da):12,367
Last modified:June 1, 1998 - v1
Checksum:i6A530D52E51FED8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20G → E in AAC95529 (PubMed:9852069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024637 mRNA. Translation: AAD09438.1.
AF077829 mRNA. Translation: AAC95529.1.
BC056450 mRNA. Translation: AAH56450.1.
CCDSiCCDS21089.1.
RefSeqiNP_035792.1. NM_011662.2.
UniGeneiMm.46301.

Genome annotation databases

EnsembliENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579.
GeneIDi22177.
KEGGimmu:22177.
UCSCiuc009gef.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024637 mRNA. Translation: AAD09438.1.
AF077829 mRNA. Translation: AAC95529.1.
BC056450 mRNA. Translation: AAH56450.1.
CCDSiCCDS21089.1.
RefSeqiNP_035792.1. NM_011662.2.
UniGeneiMm.46301.

3D structure databases

ProteinModelPortaliO54885.
SMRiO54885.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204396. 5 interactors.
DIPiDIP-29746N.
STRINGi10090.ENSMUSP00000032800.

PTM databases

iPTMnetiO54885.
PhosphoSitePlusiO54885.

Proteomic databases

PaxDbiO54885.
PeptideAtlasiO54885.
PRIDEiO54885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579.
GeneIDi22177.
KEGGimmu:22177.
UCSCiuc009gef.1. mouse.

Organism-specific databases

CTDi7305.
MGIiMGI:1277211. Tyrobp.

Phylogenomic databases

eggNOGiENOG410J1XS. Eukaryota.
ENOG410Z4TN. LUCA.
GeneTreeiENSGT00390000016786.
HOGENOMiHOG000056440.
HOVERGENiHBG061468.
InParanoidiO54885.
KOiK07992.
OrthoDBiEOG091G0X44.
PhylomeDBiO54885.
TreeFamiTF336898.

Enzyme and pathway databases

ReactomeiR-MMU-2172127. DAP12 interactions.
R-MMU-2424491. DAP12 signaling.
R-MMU-391160. Signal regulatory protein (SIRP) family interactions.
R-MMU-416700. Other semaphorin interactions.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO54885.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030579.
CleanExiMM_TYROBP.
ExpressionAtlasiO54885. baseline and differential.
GenevisibleiO54885. MM.

Family and domain databases

InterProiIPR026200. Tyrobp.
[Graphical view]
PANTHERiPTHR17554. PTHR17554. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTYOBP_MOUSE
AccessioniPrimary (citable) accession number: O54885
Secondary accession number(s): O88603
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.