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Protein

TYRO protein tyrosine kinase-binding protein

Gene

Tyrobp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Important for interaction with transmembrane receptorsBy similarity1

GO - Molecular functioni

GO - Biological processi

  • integrin-mediated signaling pathway Source: UniProtKB
  • macrophage activation involved in immune response Source: UniProtKB
  • neutrophil activation involved in immune response Source: UniProtKB
  • regulation of osteoclast development Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-2172127 DAP12 interactions
R-MMU-2424491 DAP12 signaling
R-MMU-391160 Signal regulatory protein family interactions
R-MMU-416700 Other semaphorin interactions
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
TYRO protein tyrosine kinase-binding protein
Alternative name(s):
DNAX-activation protein 12
Killer-activating receptor-associated protein
Short name:
KAR-associated protein
Gene namesi
Name:Tyrobp
Synonyms:Dap12, Karap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277211 Tyrobp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 42ExtracellularSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Topological domaini64 – 114CytoplasmicSequence analysisAdd BLAST51

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002260522 – 114TYRO protein tyrosine kinase-binding proteinAdd BLAST93

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37InterchainBy similarity
Modified residuei92PhosphotyrosineCombined sources1
Modified residuei103PhosphotyrosineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated.

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO54885
PeptideAtlasiO54885
PRIDEiO54885

PTM databases

iPTMnetiO54885
PhosphoSitePlusiO54885

Expressioni

Gene expression databases

BgeeiENSMUSG00000030579
CleanExiMM_TYROBP
ExpressionAtlasiO54885 baseline and differential
GenevisibleiO54885 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with SIGLEC14 (By similarity). Interacts with CLECSF5 (PubMed:10449773). Interacts with CD300LB and CD300D (PubMed:12874256, PubMed:12893283, PubMed:17202337, PubMed:17928527). Interacts with CD300E. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophils and macrophages integrin-mediated activation (PubMed:17086186). Interacts (via transmembrane domain) with KLRK1 isoform 2 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface expression and induce NK cell-mediated cytotoxicity (PubMed:12426564, PubMed:12426565, PubMed:15294961). Interacts with KLRC2 (By similarity).By similarity9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204396, 5 interactors
DIPiDIP-29746N
IntActiO54885, 4 interactors
STRINGi10090.ENSMUSP00000032800

Structurei

3D structure databases

ProteinModelPortaliO54885
SMRiO54885
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 109ITAMAdd BLAST29

Sequence similaritiesi

Belongs to the TYROBP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J1XS Eukaryota
ENOG410Z4TN LUCA
GeneTreeiENSGT00390000016786
HOGENOMiHOG000056440
HOVERGENiHBG061468
InParanoidiO54885
KOiK07992
OrthoDBiEOG091G0X44
PhylomeDBiO54885
TreeFamiTF336898

Family and domain databases

InterProiView protein in InterPro
IPR026200 Tyrobp
PANTHERiPTHR17554 PTHR17554, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGALEPSWCL LFLPVLLTVG GLSPVQAQSD TFPRCDCSSV SPGVLAGIVL
60 70 80 90 100
GDLVLTLLIA LAVYSLGRLV SRGQGTAEGT RKQHIAETES PYQELQGQRP
110
EVYSDLNTQR QYYR
Length:114
Mass (Da):12,367
Last modified:June 1, 1998 - v1
Checksum:i6A530D52E51FED8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20G → E in AAC95529 (PubMed:9852069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024637 mRNA Translation: AAD09438.1
AF077829 mRNA Translation: AAC95529.1
BC056450 mRNA Translation: AAH56450.1
CCDSiCCDS21089.1
RefSeqiNP_035792.1, NM_011662.2
UniGeneiMm.46301

Genome annotation databases

EnsembliENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579
GeneIDi22177
KEGGimmu:22177
UCSCiuc009gef.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTYOBP_MOUSE
AccessioniPrimary (citable) accession number: O54885
Secondary accession number(s): O88603
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health