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Protein

Guanylate cyclase soluble subunit beta-1

Gene

Gucy1b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.By similarity

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

Cofactori

hemeBy similarityNote: Binds 1 or 2 heme groups per heterodimer. Heme is required for responding to nitric oxide, but not for catalytic activity.By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron (heme proximal ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to nitric oxide Source: UniProtKB
  • cGMP biosynthetic process Source: UniProtKB
  • cGMP-mediated signaling Source: SynGO
  • nitric oxide-cGMP-mediated signaling pathway Source: UniProtKB
  • trans-synaptic signaling by nitric oxide, modulating synaptic transmission Source: SynGO

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-1 (EC:4.6.1.2By similarity)
Short name:
GCS-beta-1
Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name:
GCS-beta-3
Soluble guanylate cyclase small subunit
Gene namesi
Name:Gucy1b1
Synonyms:Gucy1b3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1860604 Gucy1b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741171 – 620Guanylate cyclase soluble subunit beta-1Add BLAST620

Proteomic databases

MaxQBiO54865
PaxDbiO54865
PeptideAtlasiO54865
PRIDEiO54865

PTM databases

iPTMnetiO54865
PhosphoSitePlusiO54865

Expressioni

Gene expression databases

BgeeiENSMUSG00000028005
CleanExiMM_GUCY1B3
ExpressionAtlasiO54865 baseline and differential
GenevisibleiO54865 MM

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1A1. Can also form inactive homodimers in vitro.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO54865, 1 interactor
MINTiO54865
STRINGi10090.ENSMUSP00000029635

Structurei

3D structure databases

ProteinModelPortaliO54865
SMRiO54865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 554Guanylate cyclasePROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000220903
HOVERGENiHBG051715
InParanoidiO54865
KOiK12319
OMAiEFLCEAP
OrthoDBiEOG091G07GF
PhylomeDBiO54865
TreeFamiTF351403

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
3.90.1520.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR038158 H-NOX_domain_sf
IPR011644 Heme_NO-bd
IPR011645 HNOB_dom_associated
IPR024096 NO_sig/Golgi_transp_ligand-bd
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07700 HNOB, 1 hit
PF07701 HNOBA, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF111126 SSF111126, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O54865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD
60 70 80 90 100
LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN
110 120 130 140 150
LDALHDHLAT IYPGMRAPSF RCTDAEKGKG LILHYYSERE GLQDIVIGII
160 170 180 190 200
KTVAQQIHGT EIDMKVIQQR NEECDHTQFL IEEKESKEED FYEDLDRFEE
210 220 230 240 250
NGTQESRISP YTFCKAFPFH IIFDRNLVVT QCGNAIYRVL PQLQPGNCSL
260 270 280 290 300
LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGAE
310 320 330 340 350
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR
360 370 380 390 400
DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP
410 420 430 440 450
SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL
460 470 480 490 500
NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA
510 520 530 540 550
LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT
560 570 580 590 600
SRTETTGEKG KINVSEYTYR CLMSPENSDP LFHLEHRGPV SMKGKKEPMQ
610 620
VWFLSRKNTG TEETNEEDEN
Length:620
Mass (Da):70,598
Last modified:June 1, 1998 - v1
Checksum:iEED89ABF539F4EF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020339 mRNA Translation: AAB94876.1
AF297083 mRNA Translation: AAG17447.1
CCDSiCCDS38460.1
RefSeqiNP_059497.1, NM_017469.4
UniGeneiMm.9445

Genome annotation databases

EnsembliENSMUST00000029635; ENSMUSP00000029635; ENSMUSG00000028005
GeneIDi54195
KEGGimmu:54195
UCSCiuc008por.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGCYB1_MOUSE
AccessioniPrimary (citable) accession number: O54865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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