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O54863

- TSSK2_MOUSE

UniProt

O54863 - TSSK2_MOUSE

Protein

Testis-specific serine/threonine-protein kinase 2

Gene

Tssk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.4 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATPPROSITE-ProRule annotation
    Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. protein autophosphorylation Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB
    4. spermatid development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.1)
    Short name:
    TSK-2
    Short name:
    TSK2
    Short name:
    TSSK-2
    Short name:
    Testis-specific kinase 2
    Alternative name(s):
    Serine/threonine-protein kinase 22B
    Gene namesi
    Name:Tssk2
    Synonyms:Stk22b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1347559. Tssk2.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Cytoplasmic vesiclesecretory vesicleacrosome
    Note: Present in the cytoplasm of elongating spermatids. In spermatozoa, localizes in the equatorial segment, neck, the midpiece and in a specific sperm head compartment. In spermatids, concentrates in centrioles during flagellogenesis. Localizes in the tail and acrosomal regions of epididymal sperm.

    GO - Cellular componenti

    1. acrosomal vesicle Source: UniProtKB
    2. centriole Source: UniProtKB
    3. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

    Pathology & Biotechi

    Disruption phenotypei

    Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Testis-specific serine/threonine-protein kinase 2PRO_0000086769Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO54863.

    PTM databases

    PhosphoSiteiO54863.

    Expressioni

    Tissue specificityi

    Testis-specific. Expressed only in the spermatids postmeiotically at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules. Also present in the epididymal sperm (at protein level).4 Publications

    Developmental stagei

    First expression detected at 3.5 to 4 weeks.

    Gene expression databases

    BgeeiO54863.
    CleanExiMM_TSSK2.
    GenevestigatoriO54863.

    Interactioni

    Subunit structurei

    Interacts with TSSK1B.2 Publications

    Protein-protein interaction databases

    IntActiO54863. 1 interaction.
    STRINGi10090.ENSMUSP00000051035.

    Structurei

    3D structure databases

    ProteinModelPortaliO54863.
    SMRiO54863. Positions 7-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 272261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115122.
    HOGENOMiHOG000233016.
    HOVERGENiHBG101110.
    InParanoidiQ6P8M9.
    KOiK08811.
    OMAiSLAVKYC.
    OrthoDBiEOG7P8P8F.
    PhylomeDBiO54863.
    TreeFamiTF352374.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54863-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDAAVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT    50
    DFVERFLPRE MDILATVNHR SIIKTYEIFE TSDGRIYIVM ELGVQGDLLE 100
    FIKCRGALHE DVARKMFRQL SSAVKYCHDL DVVHRDLKCE NLLLDKDFNI 150
    KLSDFGFSKR CLRDGSGRIV LSKTFCGSAA YAAPEVLQGI PYQPKVYDIW 200
    SLGVILYIMV CGSMPYDDSD IKKMLRIQKE HRVDFPRSKN LTGECKDLIY 250
    RILQPDVNRR LHIDEILSHS WLQPPKPKAM SSASFKREGE GKYRADCKLD 300
    TRPGSRPEHR PDHKLATKPQ QRMLVTPENE DRMEDRLAET SRAKDHHISG 350
    AEVEKAST 358
    Length:358
    Mass (Da):41,022
    Last modified:September 21, 2011 - v2
    Checksum:i551F2AAEB0BBAF31
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti100 – 1001E → T in AAC03367. (PubMed:9412477)Curated
    Sequence conflicti113 – 1142AR → GG in AAC03367. (PubMed:9412477)Curated
    Sequence conflicti120 – 1201L → V in AAC03367. (PubMed:9412477)Curated
    Sequence conflicti160 – 1601R → G in AAC03367. (PubMed:9412477)Curated
    Sequence conflicti187 – 1871L → R in AAC03367. (PubMed:9412477)Curated
    Sequence conflicti224 – 2241Missing in AAC03367. (PubMed:9412477)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019926 mRNA. Translation: AAC03367.1.
    BC061175 mRNA. Translation: AAH61175.1.
    CH466521 Genomic DNA. Translation: EDK97491.1.
    CCDSiCCDS28012.1.
    RefSeqiNP_033462.2. NM_009436.2.
    UniGeneiMm.310201.

    Genome annotation databases

    EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521.
    GeneIDi22115.
    KEGGimmu:22115.
    UCSCiuc007yml.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019926 mRNA. Translation: AAC03367.1 .
    BC061175 mRNA. Translation: AAH61175.1 .
    CH466521 Genomic DNA. Translation: EDK97491.1 .
    CCDSi CCDS28012.1.
    RefSeqi NP_033462.2. NM_009436.2.
    UniGenei Mm.310201.

    3D structure databases

    ProteinModelPortali O54863.
    SMRi O54863. Positions 7-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O54863. 1 interaction.
    STRINGi 10090.ENSMUSP00000051035.

    PTM databases

    PhosphoSitei O54863.

    Proteomic databases

    PRIDEi O54863.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055374 ; ENSMUSP00000051035 ; ENSMUSG00000045521 .
    GeneIDi 22115.
    KEGGi mmu:22115.
    UCSCi uc007yml.1. mouse.

    Organism-specific databases

    CTDi 23617.
    MGIi MGI:1347559. Tssk2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115122.
    HOGENOMi HOG000233016.
    HOVERGENi HBG101110.
    InParanoidi Q6P8M9.
    KOi K08811.
    OMAi SLAVKYC.
    OrthoDBi EOG7P8P8F.
    PhylomeDBi O54863.
    TreeFami TF352374.

    Miscellaneous databases

    NextBioi 301967.
    PROi O54863.
    SOURCEi Search...

    Gene expression databases

    Bgeei O54863.
    CleanExi MM_TSSK2.
    Genevestigatori O54863.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel family of serine/threonine kinases participating in spermiogenesis."
      Kueng P., Nikolova Z., Djonov V., Hemphill A., Rohrbach V., Boehlen D., Zuercher G., Andres A.-C., Ziemiecki A.
      J. Cell Biol. 139:1851-1859(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis."
      Nayak S., Galili N., Buck C.A.
      Mech. Dev. 74:171-174(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    5. "A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice."
      Zuercher G., Rohrbach V., Andres A.-C., Ziemiecki A.
      Mech. Dev. 93:175-177(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TSSK1B.
    6. "Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2."
      Zhang Z., Shen X., Jones B.H., Xu B., Herr J.C., Strauss J.F. III
      Biol. Reprod. 79:75-83(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPAG16.
    7. Cited for: SUBCELLULAR LOCATION.
    8. "Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency."
      Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S., Strauss J.F. III, Flickinger C.J., Herr J.C.
      Dev. Biol. 319:211-222(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    9. "Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases."
      Shang P., Baarends W.M., Hoogerbrugge J., Ooms M.P., van Cappellen W.A., de Jong A.A., Dohle G.R., van Eenennaam H., Gossen J.A., Grootegoed J.A.
      J. Cell Sci. 123:331-339(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
    10. "Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
      Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
      Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTSSK2_MOUSE
    AccessioniPrimary (citable) accession number: O54863
    Secondary accession number(s): Q6P8M9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3