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O54863 (TSSK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testis-specific serine/threonine-protein kinase 2

Short name=TSK-2
Short name=TSK2
Short name=TSSK-2
Short name=Testis-specific kinase 2
EC=2.7.11.1
Alternative name(s):
Serine/threonine-protein kinase 22B
Gene names
Name:Tssk2
Synonyms:Stk22b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body. Ref.1 Ref.6 Ref.8 Ref.9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by phosphorylation on Thr-174, potentially by autophosphorylation By similarity.

Subunit structure

Interacts with TSSK1B. Ref.5 Ref.6

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Cytoplasmic vesiclesecretory vesicleacrosome. Note: Present in the cytoplasm of elongating spermatids. In spermatozoa, localizes in the equatorial segment, neck, the midpiece and in a specific sperm head compartment. In spermatids, concentrates in centrioles during flagellogenesis. Localizes in the tail and acrosomal regions of epididymal sperm. Ref.1 Ref.4 Ref.6 Ref.7 Ref.9 Ref.10

Tissue specificity

Testis-specific. Expressed only in the spermatids postmeiotically at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules. Also present in the epididymal sperm (at protein level). Ref.1 Ref.4 Ref.8 Ref.10

Developmental stage

First expression detected at 3.5 to 4 weeks.

Post-translational modification

Autophosphorylated By similarity.

Disruption phenotype

Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath. Ref.8 Ref.9

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Testis-specific serine/threonine-protein kinase 2
PRO_0000086769

Regions

Domain12 – 272261Protein kinase
Nucleotide binding18 – 269ATP By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site411ATP By similarity

Experimental info

Sequence conflict1001E → T in AAC03367. Ref.1
Sequence conflict113 – 1142AR → GG in AAC03367. Ref.1
Sequence conflict1201L → V in AAC03367. Ref.1
Sequence conflict1601R → G in AAC03367. Ref.1
Sequence conflict1871L → R in AAC03367. Ref.1
Sequence conflict2241Missing in AAC03367. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O54863 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 551F2AAEB0BBAF31

FASTA35841,022
        10         20         30         40         50         60 
MDDAAVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT DFVERFLPRE 

        70         80         90        100        110        120 
MDILATVNHR SIIKTYEIFE TSDGRIYIVM ELGVQGDLLE FIKCRGALHE DVARKMFRQL 

       130        140        150        160        170        180 
SSAVKYCHDL DVVHRDLKCE NLLLDKDFNI KLSDFGFSKR CLRDGSGRIV LSKTFCGSAA 

       190        200        210        220        230        240 
YAAPEVLQGI PYQPKVYDIW SLGVILYIMV CGSMPYDDSD IKKMLRIQKE HRVDFPRSKN 

       250        260        270        280        290        300 
LTGECKDLIY RILQPDVNRR LHIDEILSHS WLQPPKPKAM SSASFKREGE GKYRADCKLD 

       310        320        330        340        350 
TRPGSRPEHR PDHKLATKPQ QRMLVTPENE DRMEDRLAET SRAKDHHISG AEVEKAST 

« Hide

References

« Hide 'large scale' references
[1]"A novel family of serine/threonine kinases participating in spermiogenesis."
Kueng P., Nikolova Z., Djonov V., Hemphill A., Rohrbach V., Boehlen D., Zuercher G., Andres A.-C., Ziemiecki A.
J. Cell Biol. 139:1851-1859(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis."
Nayak S., Galili N., Buck C.A.
Mech. Dev. 74:171-174(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice."
Zuercher G., Rohrbach V., Andres A.-C., Ziemiecki A.
Mech. Dev. 93:175-177(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TSSK1B.
[6]"Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2."
Zhang Z., Shen X., Jones B.H., Xu B., Herr J.C., Strauss J.F. III
Biol. Reprod. 79:75-83(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPAG16.
[7]"TSKS concentrates in spermatid centrioles during flagellogenesis."
Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S., Strauss J.F. III, Flickinger C.J., Herr J.C.
Dev. Biol. 319:201-210(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency."
Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S., Strauss J.F. III, Flickinger C.J., Herr J.C.
Dev. Biol. 319:211-222(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]"Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases."
Shang P., Baarends W.M., Hoogerbrugge J., Ooms M.P., van Cappellen W.A., de Jong A.A., Dohle G.R., van Eenennaam H., Gossen J.A., Grootegoed J.A.
J. Cell Sci. 123:331-339(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
[10]"Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019926 mRNA. Translation: AAC03367.1.
BC061175 mRNA. Translation: AAH61175.1.
CH466521 Genomic DNA. Translation: EDK97491.1.
RefSeqNP_033462.2. NM_009436.2.
UniGeneMm.310201.

3D structure databases

ProteinModelPortalO54863.
SMRO54863. Positions 10-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO54863. 1 interaction.
STRING10090.ENSMUSP00000051035.

PTM databases

PhosphoSiteO54863.

Proteomic databases

PRIDEO54863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521.
GeneID22115.
KEGGmmu:22115.
UCSCuc007yml.1. mouse.

Organism-specific databases

CTD23617.
MGIMGI:1347559. Tssk2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000115122.
HOGENOMHOG000233016.
HOVERGENHBG101110.
InParanoidQ6P8M9.
KOK08811.
OMASLAVKYC.
OrthoDBEOG7P8P8F.
PhylomeDBO54863.
TreeFamTF352374.

Gene expression databases

BgeeO54863.
CleanExMM_TSSK2.
GenevestigatorO54863.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301967.
PROO54863.
SOURCESearch...

Entry information

Entry nameTSSK2_MOUSE
AccessionPrimary (citable) accession number: O54863
Secondary accession number(s): Q6P8M9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot