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Protein

Testis-specific serine/threonine-protein kinase 2

Gene

Tssk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.1)
Short name:
TSK-2
Short name:
TSK2
Short name:
TSSK-2
Short name:
Testis-specific kinase 2
Alternative name(s):
Serine/threonine-protein kinase 22B
Gene namesi
Name:Tssk2
Synonyms:Stk22b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1347559. Tssk2.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB
  • centriole Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867691 – 358Testis-specific serine/threonine-protein kinase 2Add BLAST358

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54863.
PRIDEiO54863.

PTM databases

iPTMnetiO54863.
PhosphoSitePlusiO54863.

Expressioni

Tissue specificityi

Testis-specific. Expressed only in the spermatids postmeiotically at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules. Also present in the epididymal sperm (at protein level).4 Publications

Developmental stagei

First expression detected at 3.5 to 4 weeks.

Gene expression databases

BgeeiENSMUSG00000045521.
CleanExiMM_TSSK2.
GenevisibleiO54863. MM.

Interactioni

Subunit structurei

Interacts with TSSK1B.2 Publications

Protein-protein interaction databases

IntActiO54863. 1 interactor.
STRINGi10090.ENSMUSP00000051035.

Structurei

3D structure databases

ProteinModelPortaliO54863.
SMRiO54863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiO54863.
KOiK08811.
OMAiATVNHGS.
OrthoDBiEOG091G0EZI.
PhylomeDBiO54863.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDAAVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT
60 70 80 90 100
DFVERFLPRE MDILATVNHR SIIKTYEIFE TSDGRIYIVM ELGVQGDLLE
110 120 130 140 150
FIKCRGALHE DVARKMFRQL SSAVKYCHDL DVVHRDLKCE NLLLDKDFNI
160 170 180 190 200
KLSDFGFSKR CLRDGSGRIV LSKTFCGSAA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSD IKKMLRIQKE HRVDFPRSKN LTGECKDLIY
260 270 280 290 300
RILQPDVNRR LHIDEILSHS WLQPPKPKAM SSASFKREGE GKYRADCKLD
310 320 330 340 350
TRPGSRPEHR PDHKLATKPQ QRMLVTPENE DRMEDRLAET SRAKDHHISG

AEVEKAST
Length:358
Mass (Da):41,022
Last modified:September 21, 2011 - v2
Checksum:i551F2AAEB0BBAF31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100E → T in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti113 – 114AR → GG in AAC03367 (PubMed:9412477).Curated2
Sequence conflicti120L → V in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti160R → G in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti187L → R in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti224Missing in AAC03367 (PubMed:9412477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA. Translation: AAC03367.1.
BC061175 mRNA. Translation: AAH61175.1.
CH466521 Genomic DNA. Translation: EDK97491.1.
CCDSiCCDS28012.1.
RefSeqiNP_033462.2. NM_009436.2.
UniGeneiMm.310201.

Genome annotation databases

EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521.
GeneIDi22115.
KEGGimmu:22115.
UCSCiuc007yml.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA. Translation: AAC03367.1.
BC061175 mRNA. Translation: AAH61175.1.
CH466521 Genomic DNA. Translation: EDK97491.1.
CCDSiCCDS28012.1.
RefSeqiNP_033462.2. NM_009436.2.
UniGeneiMm.310201.

3D structure databases

ProteinModelPortaliO54863.
SMRiO54863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54863. 1 interactor.
STRINGi10090.ENSMUSP00000051035.

PTM databases

iPTMnetiO54863.
PhosphoSitePlusiO54863.

Proteomic databases

PaxDbiO54863.
PRIDEiO54863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521.
GeneIDi22115.
KEGGimmu:22115.
UCSCiuc007yml.1. mouse.

Organism-specific databases

CTDi23617.
MGIiMGI:1347559. Tssk2.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiO54863.
KOiK08811.
OMAiATVNHGS.
OrthoDBiEOG091G0EZI.
PhylomeDBiO54863.
TreeFamiTF352374.

Miscellaneous databases

PROiO54863.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045521.
CleanExiMM_TSSK2.
GenevisibleiO54863. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK2_MOUSE
AccessioniPrimary (citable) accession number: O54863
Secondary accession number(s): Q6P8M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.