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O54863

- TSSK2_MOUSE

UniProt

O54863 - TSSK2_MOUSE

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Protein

Testis-specific serine/threonine-protein kinase 2

Gene

Tssk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. protein autophosphorylation Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
  4. spermatid development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.1)
Short name:
TSK-2
Short name:
TSK2
Short name:
TSSK-2
Short name:
Testis-specific kinase 2
Alternative name(s):
Serine/threonine-protein kinase 22B
Gene namesi
Name:Tssk2
Synonyms:Stk22b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1347559. Tssk2.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Cytoplasmic vesiclesecretory vesicleacrosome
Note: Present in the cytoplasm of elongating spermatids. In spermatozoa, localizes in the equatorial segment, neck, the midpiece and in a specific sperm head compartment. In spermatids, concentrates in centrioles during flagellogenesis. Localizes in the tail and acrosomal regions of epididymal sperm.

GO - Cellular componenti

  1. acrosomal vesicle Source: UniProtKB
  2. centriole Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Testis-specific serine/threonine-protein kinase 2PRO_0000086769Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO54863.

PTM databases

PhosphoSiteiO54863.

Expressioni

Tissue specificityi

Testis-specific. Expressed only in the spermatids postmeiotically at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules. Also present in the epididymal sperm (at protein level).4 Publications

Developmental stagei

First expression detected at 3.5 to 4 weeks.

Gene expression databases

BgeeiO54863.
CleanExiMM_TSSK2.
GenevestigatoriO54863.

Interactioni

Subunit structurei

Interacts with TSSK1B.2 Publications

Protein-protein interaction databases

IntActiO54863. 1 interaction.
STRINGi10090.ENSMUSP00000051035.

Structurei

3D structure databases

ProteinModelPortaliO54863.
SMRiO54863. Positions 10-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 272261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiO54863.
KOiK08811.
OMAiSLAVKYC.
OrthoDBiEOG7P8P8F.
PhylomeDBiO54863.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54863-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDAAVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT
60 70 80 90 100
DFVERFLPRE MDILATVNHR SIIKTYEIFE TSDGRIYIVM ELGVQGDLLE
110 120 130 140 150
FIKCRGALHE DVARKMFRQL SSAVKYCHDL DVVHRDLKCE NLLLDKDFNI
160 170 180 190 200
KLSDFGFSKR CLRDGSGRIV LSKTFCGSAA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSD IKKMLRIQKE HRVDFPRSKN LTGECKDLIY
260 270 280 290 300
RILQPDVNRR LHIDEILSHS WLQPPKPKAM SSASFKREGE GKYRADCKLD
310 320 330 340 350
TRPGSRPEHR PDHKLATKPQ QRMLVTPENE DRMEDRLAET SRAKDHHISG

AEVEKAST
Length:358
Mass (Da):41,022
Last modified:September 21, 2011 - v2
Checksum:i551F2AAEB0BBAF31
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001E → T in AAC03367. (PubMed:9412477)Curated
Sequence conflicti113 – 1142AR → GG in AAC03367. (PubMed:9412477)Curated
Sequence conflicti120 – 1201L → V in AAC03367. (PubMed:9412477)Curated
Sequence conflicti160 – 1601R → G in AAC03367. (PubMed:9412477)Curated
Sequence conflicti187 – 1871L → R in AAC03367. (PubMed:9412477)Curated
Sequence conflicti224 – 2241Missing in AAC03367. (PubMed:9412477)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA. Translation: AAC03367.1.
BC061175 mRNA. Translation: AAH61175.1.
CH466521 Genomic DNA. Translation: EDK97491.1.
CCDSiCCDS28012.1.
RefSeqiNP_033462.2. NM_009436.2.
UniGeneiMm.310201.

Genome annotation databases

EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521.
GeneIDi22115.
KEGGimmu:22115.
UCSCiuc007yml.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA. Translation: AAC03367.1 .
BC061175 mRNA. Translation: AAH61175.1 .
CH466521 Genomic DNA. Translation: EDK97491.1 .
CCDSi CCDS28012.1.
RefSeqi NP_033462.2. NM_009436.2.
UniGenei Mm.310201.

3D structure databases

ProteinModelPortali O54863.
SMRi O54863. Positions 10-307.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O54863. 1 interaction.
STRINGi 10090.ENSMUSP00000051035.

PTM databases

PhosphoSitei O54863.

Proteomic databases

PRIDEi O54863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000055374 ; ENSMUSP00000051035 ; ENSMUSG00000045521 .
GeneIDi 22115.
KEGGi mmu:22115.
UCSCi uc007yml.1. mouse.

Organism-specific databases

CTDi 23617.
MGIi MGI:1347559. Tssk2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120521.
HOGENOMi HOG000233016.
HOVERGENi HBG101110.
InParanoidi O54863.
KOi K08811.
OMAi SLAVKYC.
OrthoDBi EOG7P8P8F.
PhylomeDBi O54863.
TreeFami TF352374.

Miscellaneous databases

NextBioi 301967.
PROi O54863.
SOURCEi Search...

Gene expression databases

Bgeei O54863.
CleanExi MM_TSSK2.
Genevestigatori O54863.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel family of serine/threonine kinases participating in spermiogenesis."
    Kueng P., Nikolova Z., Djonov V., Hemphill A., Rohrbach V., Boehlen D., Zuercher G., Andres A.-C., Ziemiecki A.
    J. Cell Biol. 139:1851-1859(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis."
    Nayak S., Galili N., Buck C.A.
    Mech. Dev. 74:171-174(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice."
    Zuercher G., Rohrbach V., Andres A.-C., Ziemiecki A.
    Mech. Dev. 93:175-177(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TSSK1B.
  6. "Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2."
    Zhang Z., Shen X., Jones B.H., Xu B., Herr J.C., Strauss J.F. III
    Biol. Reprod. 79:75-83(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPAG16.
  7. Cited for: SUBCELLULAR LOCATION.
  8. "Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency."
    Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S., Strauss J.F. III, Flickinger C.J., Herr J.C.
    Dev. Biol. 319:211-222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  9. "Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases."
    Shang P., Baarends W.M., Hoogerbrugge J., Ooms M.P., van Cappellen W.A., de Jong A.A., Dohle G.R., van Eenennaam H., Gossen J.A., Grootegoed J.A.
    J. Cell Sci. 123:331-339(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
  10. "Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
    Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
    Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTSSK2_MOUSE
AccessioniPrimary (citable) accession number: O54863
Secondary accession number(s): Q6P8M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: September 21, 2011
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3