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O54853 (KCNH6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 6
Alternative name(s):
Ether-a-go-go-related gene potassium channel 2
Short name=ERG-2
Short name=Eag-related protein 2
Short name=Ether-a-go-go-related protein 2
Voltage-gated potassium channel subunit Kv11.2
Gene names
Name:Kcnh6
Synonyms:Erg2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length950 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH2/ERG1 and KCNH7/ERG3.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in celiac and superior mesenteric ganglia, but not detected in brain or in heart. Detected at low levels in retina. Also found in pituitary. Also found in the olfactory bulb (granular and mitral cell layers) (Ref.4). Ref.2 Ref.4

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.2/KCNH6 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 950950Potassium voltage-gated channel subfamily H member 6
PRO_0000054014

Regions

Topological domain1 – 261261Cytoplasmic Potential
Transmembrane262 – 28221Helical; Name=Segment S1; Potential
Topological domain283 – 29816Extracellular Potential
Transmembrane299 – 31921Helical; Name=Segment S2; Potential
Topological domain320 – 34021Cytoplasmic Potential
Transmembrane341 – 36121Helical; Name=Segment S3; Potential
Topological domain362 – 3709Extracellular Potential
Transmembrane371 – 39121Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain392 – 3987Cytoplasmic Potential
Transmembrane399 – 41921Helical; Name=Segment S5; Potential
Topological domain420 – 46344Extracellular Potential
Intramembrane464 – 48421Pore-forming; Name=Segment H5; Potential
Topological domain485 – 4906Extracellular Potential
Transmembrane491 – 51121Helical; Name=Segment S6; Potential
Topological domain512 – 950439Cytoplasmic Potential
Domain41 – 7030PAS
Domain92 – 14453PAC
Nucleotide binding594 – 711118cNMP
Motif476 – 4816Selectivity filter By similarity

Experimental info

Mutagenesis4801G → S: Dominant negative mutant; abolishes ERG current. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O54853 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: AB5AE674B08776F0

FASTA950105,706
        10         20         30         40         50         60 
MPVRRGHVAP QNTYLDTIIR KFEGQSRKFL IANAQMENCA IIYCNDGFCE LFGYSRVEVM 

        70         80         90        100        110        120 
QRPCTCDFLT GPNTPSSAVS RLAQALLGAE ECKVDILYYR KDASSFRCLV DVVPVKNEDG 

       130        140        150        160        170        180 
AVIMFILNFE DLAQLLAKSS SRSLTQRLLS HSFLGSEGSH SRPSGQGPGP GRGKYRTVSQ 

       190        200        210        220        230        240 
IPQFTLNFVE FNLEKHRSGS TTEIEIIAPH KVVERTQNVT EKVTQVLSLG ADVLPEYKLQ 

       250        260        270        280        290        300 
APRIHRGTIL HYSPFKAVWD WLILLLVIYT AVFTPYSAAF LLSDQDESQR GTCGYTCSPL 

       310        320        330        340        350        360 
TVVDLIVDIM FVVDIVINFR TTYVNTNDEV VSHPRRIAVH YFKGWFLIDM VAAIPFDLLI 

       370        380        390        400        410        420 
FRTGSDETTT LIGLLKTARL LRLVRVARKL DRYSEYGAAV LFLLMCTFAL IAHWLACIWY 

       430        440        450        460        470        480 
AIGNVERPYL EPKIGWLDSL GAQLGKQYNG SDPASGPSVQ DKYVTALYFT FSSLTSVGFG 

       490        500        510        520        530        540 
NVSPNTNSEK VFSICVMLIG SLMYASIFGN VSAIIQRLYS GTARYHTQML RVKEFIRFHQ 

       550        560        570        580        590        600 
IPNPLRQRLE EYFQHAWSYT NGIDMNAVLK GFPECLQADI CLHLHRALLQ HCPAFRGASK 

       610        620        630        640        650        660 
GCLRALAVKF KTTHAPPGDT LVHLGDVLST LYFISRGSIE ILRDDVVVAI LGKNDIFGEP 

       670        680        690        700        710        720 
ASLHARPGKS SADVRALTYC DLHKIHRADL LEVLDMYPAF ADTFWNKLEV TFNLRDADGG 

       730        740        750        760        770        780 
LQSTPRQAPG HQDPQGFFLN DSQSGAAPSL SISDTSALWP ELLQQMPPSP PNPRQDLDCW 

       790        800        810        820        830        840 
HRELGFKLEQ LQAQMNRLES RVSSDLSRIL QLLQHPQGRP SYILGASASS DLASFPETSV 

       850        860        870        880        890        900 
TRSSESTLLV GHVPSAQTLS YGDLDDHIQT PRNFSPRTPH VAMAMDKTLV PSSEQEQPGG 

       910        920        930        940        950 
LLSPLASPLR PLEVPGLGGS RFPSLPEHLS SVPKQLEFQR HGSDPGFTRS 

« Hide

References

[1]"Identification of two nervous system-specific members of the erg potassium channel gene family."
Shi W., Wymore R.S., Wang H.-S., Pan Z., Cohen I.S., McKinnon D., Dixon J.E.
J. Neurosci. 17:9423-9432(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cervical ganglion.
[2]"Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[3]"Erg1, erg2 and erg3 K channel subunits are able to form heteromultimers."
Wimmers S., Wulfsen I., Bauer C.K., Schwarz J.R.
Pflugers Arch. 441:450-455(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KCNH2 AND KCNH7, MUTAGENESIS OF GLY-480.
[4]"Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain."
Saganich M.J., Machado E., Rudy B.
J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF016192 mRNA. Translation: AAB94742.1.
RefSeqNP_446389.1. NM_053937.1.
UniGeneRn.10875.

3D structure databases

ProteinModelPortalO54853.
SMRO54853. Positions 26-130, 421-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000010816.

Chemistry

GuidetoPHARMACOLOGY573.

Protein family/group databases

TCDB1.A.1.20.2. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteO54853.

Proteomic databases

PRIDEO54853.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID116745.
KEGGrno:116745.
UCSCRGD:620304. rat.

Organism-specific databases

CTD81033.
RGD620304. Kcnh6.

Phylogenomic databases

eggNOGCOG2202.
HOGENOMHOG000230793.
HOVERGENHBG052232.
InParanoidO54853.
KOK04909.
PhylomeDBO54853.

Gene expression databases

GenevestigatorO54853.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003967. K_chnl_volt-dep_ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01470. ERGCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619690.
PROO54853.

Entry information

Entry nameKCNH6_RAT
AccessionPrimary (citable) accession number: O54853
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families