Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O54839 (EOMES_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eomesodermin homolog
Alternative name(s):
T-box brain protein 2
Short name=T-brain-2
Short name=TBR-2
Gene names
Name:Eomes
Synonyms:Tbr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length707 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in CD8+ T-cells. Ref.8

Developmental stage

Originally expressed in the trophoectoderm of the blastocyst and later in the extraembryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain. Ref.7 Ref.10

Induction

Up-regulated in CD8+ T-cells upon activation. Ref.8

Disruption phenotype

Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects. Ref.7

Sequence similarities

Contains 1 T-box DNA-binding domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Differentiation
Gastrulation
Immunity
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCD8-positive, alpha-beta T cell differentiation involved in immune response

Inferred from direct assay Ref.8. Source: UniProtKB

blastocyst development

Inferred from mutant phenotype PubMed 15788452. Source: MGI

cardioblast differentiation

Inferred from mutant phenotype PubMed 21822279. Source: MGI

cell differentiation

Inferred from mutant phenotype Ref.7PubMed 15788452. Source: MGI

cell differentiation involved in embryonic placenta development

Inferred from mutant phenotype Ref.7. Source: UniProtKB

cerebral cortex neuron differentiation

Inferred from direct assay Ref.10. Source: UniProtKB

cerebral cortex regionalization

Inferred from mutant phenotype PubMed 23431145. Source: MGI

endoderm formation

Inferred from mutant phenotype Ref.9. Source: UniProtKB

endodermal cell fate specification

Inferred from mutant phenotype PubMed 21245162. Source: MGI

interferon-gamma production

Inferred from direct assay PubMed 20399120. Source: MGI

mesoderm formation

Inferred from mutant phenotype Ref.7. Source: UniProtKB

mesodermal to mesenchymal transition involved in gastrulation

Inferred from mutant phenotype Ref.9. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 22164283. Source: BHF-UCL

olfactory bulb development

Inferred from mutant phenotype PubMed 19435790. Source: MGI

positive regulation of cell differentiation

Inferred from direct assay PubMed 16325584. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 21245162. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of gene expression

Inferred from mutant phenotype PubMed 21245162. Source: MGI

regulation of neurogenesis

Inferred from mutant phenotype PubMed 18794345. Source: MGI

regulation of neuron differentiation

Inferred from mutant phenotype PubMed 18794345. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622. Source: MGI

skeletal muscle cell differentiation

Inferred from mutant phenotype PubMed 22147266. Source: MGI

stem cell maintenance

Inferred from mutant phenotype PubMed 20176728. Source: MGI

trophectodermal cell differentiation

Inferred from direct assay PubMed 16325584. Source: MGI

   Cellular_componentintracellular

Inferred from direct assay PubMed 16892058. Source: MGI

nucleus

Inferred from direct assay PubMed 15880683PubMed 19409883PubMed 19796622PubMed 23434913. Source: MGI

   Molecular_functionDNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

RNA polymerase II activating transcription factor binding

Inferred from physical interaction PubMed 22164283. Source: BHF-UCL

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 20176728. Source: MGI

RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 22164283. Source: BHF-UCL

chromatin binding

Inferred from direct assay PubMed 21822279. Source: MGI

sequence-specific DNA binding

Inferred from sequence orthology PubMed 21245162. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O54839-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O54839-2)

The sequence of this isoform differs from the canonical sequence as follows:
     463-481: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 707707Eomesodermin homolog
PRO_0000184460

Regions

DNA binding278 – 458181T-box
Region592 – 707116Required for transcription activation By similarity
Compositional bias27 – 266240Gly-rich

Natural variations

Alternative sequence463 – 48119Missing in isoform 2.
VSP_038806

Experimental info

Sequence conflict1441E → D in BAC40968. Ref.3
Sequence conflict1591A → T in BAC40968. Ref.3
Sequence conflict1781V → G in BAA83416. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 23, 2010. Version 3.
Checksum: 277AA462E214A927

FASTA70774,801
        10         20         30         40         50         60 
MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA PSPQRLDLDK 

        70         80         90        100        110        120 
ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG STSAVAKPGP PDGRKGSPCA 

       130        140        150        160        170        180 
EEELPSAATA AATARYSMDS LSSERYYLPS PGPQGSELAA PCSLFQYPAA AGAAHGPVYP 

       190        200        210        220        230        240 
ASNGARYPYG SMLPPGGFPA AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP 

       250        260        270        280        290        300 
LYGPYAGTSA AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP 

       310        320        330        340        350        360 
FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK MYVHPESPNT 

       370        380        390        400        410        420 
GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP RLHIVEVTED GVEDLNEPSK 

       430        440        450        460        470        480 
TQTFTFSETQ FIAVTAYQNT DITQLKIDHN PFAKGFRDNY DSMYTASEND RLTPSPTDSP 

       490        500        510        520        530        540 
RSHQIVPGGR YGVQNFFPEP FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL 

       550        560        570        580        590        600 
VTPVQQPVTN KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG 

       610        620        630        640        650        660 
AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD SSDSGVYNSA 

       670        680        690        700 
CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY YAFYTSP 

« Hide

Isoform 2 [UniParc].

Checksum: 59CB0B89E920BFE6
Show »

FASTA68872,681

References

« Hide 'large scale' references
[1]"A novel mammalian T-box-containing gene, Tbr2, expressed in mouse developing brain."
Kimura N., Nakashima K., Ueno M., Taga T.
Brain Res. Dev. Brain Res. 115:183-193(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1."
Ueno M., Kimura N., Nakashima K., Saito-Ohara F., Inazawa J., Taga T.
Gene 254:29-35(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
Strain: 129/Sv.
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Olfactory bulb and Spleen.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[6]"A combined analysis of genomic and primary protein structure defines the phylogenetic relationship of new members of the T-box family."
Wattler S., Russ A., Evans M., Nehls M.
Genomics 48:24-33(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 278-457.
[7]"Eomesodermin is required for mouse trophoblast development and mesoderm formation."
Russ A.P., Wattler S., Colledge W.H., Aparicio S.A.J.R., Carlton M.B.L., Pearce J.J., Barton S.C., Surani M.A., Ryan K., Nehls M.C., Wilson V., Evans M.J.
Nature 404:95-99(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN TROPHOBLAST DIFFERENTIATION, FUNCTION IN GASTRULATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[8]"Control of effector CD8+ T cell function by the transcription factor Eomesodermin."
Pearce E.L., Mullen A.C., Martins G.A., Krawczyk C.M., Hutchins A.S., Zediak V.P., Banica M., DiCioccio C.B., Gross D.A., Mao C.-A., Shen H., Cereb N., Yang S.Y., Lindsten T., Rossant J., Hunter C.A., Reiner S.L.
Science 302:1041-1043(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[9]"Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse."
Arnold S.J., Hofmann U.K., Bikoff E.K., Robertson E.J.
Development 135:501-511(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN GASTRULATION.
[10]"Tbr2 directs conversion of radial glia into basal precursors and guides neuronal amplification by indirect neurogenesis in the developing neocortex."
Sessa A., Mao C.-A., Hadjantonakis A.-K., Klein W.H., Broccoli V.
Neuron 60:56-69(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB031037 mRNA. Translation: BAA83416.1.
AB032373 Genomic DNA. Translation: BAB07808.1.
AK089817 mRNA. Translation: BAC40968.1.
AK143454 mRNA. Translation: BAE25384.1.
AC173340 Genomic DNA. No translation available.
BC094319 mRNA. Translation: AAH94319.1.
AF013281 mRNA. Translation: AAC16233.1.
CCDSCCDS23603.1. [O54839-1]
CCDS52960.1. [O54839-2]
RefSeqNP_001158261.1. NM_001164789.1. [O54839-2]
NP_034266.2. NM_010136.3. [O54839-1]
UniGeneMm.200692.

3D structure databases

ProteinModelPortalO54839.
SMRO54839. Positions 271-457.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO54839.

Proteomic databases

PRIDEO54839.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. [O54839-1]
ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446. [O54839-2]
GeneID13813.
KEGGmmu:13813.
UCSCuc009rzo.2. mouse. [O54839-1]
uc009rzp.2. mouse. [O54839-2]

Organism-specific databases

CTD8320.
MGIMGI:1201683. Eomes.

Phylogenomic databases

eggNOGNOG245070.
GeneTreeENSGT00740000115029.
HOGENOMHOG000218633.
HOVERGENHBG000578.
InParanoidQ8BN22.
KOK10173.
OMAHGPVYPA.
OrthoDBEOG7GFB4D.
PhylomeDBO54839.
TreeFamTF106341.

Gene expression databases

ArrayExpressO54839.
BgeeO54839.
CleanExMM_EOMES.
GenevestigatorO54839.

Family and domain databases

Gene3D2.60.40.820. 1 hit.
InterProIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERPTHR11267. PTHR11267. 1 hit.
PfamPF00907. T-box. 1 hit.
[Graphical view]
PRINTSPR00937. TBOX.
SMARTSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMSSF49417. SSF49417. 1 hit.
PROSITEPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284604.
PROO54839.
SOURCESearch...

Entry information

Entry nameEOMES_MOUSE
AccessionPrimary (citable) accession number: O54839
Secondary accession number(s): Q3UPL1 expand/collapse secondary AC list , Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 23, 2010
Last modified: July 9, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot