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Protein

Eomesodermin homolog

Gene

Eomes

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi278 – 458T-boxPROSITE-ProRule annotationAdd BLAST181

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • blastocyst development Source: MGI
  • brain development Source: MGI
  • cardioblast differentiation Source: MGI
  • CD8-positive, alpha-beta T cell differentiation involved in immune response Source: UniProtKB
  • cell differentiation Source: MGI
  • cell differentiation involved in embryonic placenta development Source: UniProtKB
  • cerebral cortex neuron differentiation Source: UniProtKB
  • cerebral cortex regionalization Source: MGI
  • endodermal cell fate specification Source: MGI
  • endoderm formation Source: UniProtKB
  • interferon-gamma production Source: MGI
  • mesodermal to mesenchymal transition involved in gastrulation Source: UniProtKB
  • mesoderm formation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • olfactory bulb development Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of neurogenesis Source: MGI
  • regulation of neuron differentiation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • trophectodermal cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Adaptive immunity, Differentiation, Gastrulation, Immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eomesodermin homolog
Alternative name(s):
T-box brain protein 2
Short name:
T-brain-2
Short name:
TBR-2
Gene namesi
Name:Eomes
Synonyms:Tbr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1201683. Eomes.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001844601 – 707Eomesodermin homologAdd BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineCombined sources1
Modified residuei473PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54839.
PeptideAtlasiO54839.
PRIDEiO54839.

PTM databases

iPTMnetiO54839.
PhosphoSitePlusiO54839.

Expressioni

Tissue specificityi

Expressed in CD8+ T-cells.1 Publication

Developmental stagei

Originally expressed in the trophoectoderm of the blastocyst and later in the extraembryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain.2 Publications

Inductioni

Up-regulated in CD8+ T-cells upon activation.1 Publication

Gene expression databases

BgeeiENSMUSG00000032446.
CleanExiMM_EOMES.
ExpressionAtlasiO54839. baseline and differential.
GenevisibleiO54839. MM.

Interactioni

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi199457. 2 interactors.
STRINGi10090.ENSMUSP00000035020.

Structurei

3D structure databases

ProteinModelPortaliO54839.
SMRiO54839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni592 – 707Required for transcription activationBy similarityAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 266Gly-richAdd BLAST240

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3585. Eukaryota.
ENOG410XSTS. LUCA.
GeneTreeiENSGT00740000115029.
HOGENOMiHOG000218633.
HOVERGENiHBG000578.
InParanoidiO54839.
KOiK10173.
OMAiSAQRWFV.
OrthoDBiEOG091G0PQQ.
PhylomeDBiO54839.
TreeFamiTF106341.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR032385. T-box_assoc.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
PF16176. T-box_assoc. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54839-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA
60 70 80 90 100
PSPQRLDLDK ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG
110 120 130 140 150
STSAVAKPGP PDGRKGSPCA EEELPSAATA AATARYSMDS LSSERYYLPS
160 170 180 190 200
PGPQGSELAA PCSLFQYPAA AGAAHGPVYP ASNGARYPYG SMLPPGGFPA
210 220 230 240 250
AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP LYGPYAGTSA
260 270 280 290 300
AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP
310 320 330 340 350
FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK
360 370 380 390 400
MYVHPESPNT GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP
410 420 430 440 450
RLHIVEVTED GVEDLNEPSK TQTFTFSETQ FIAVTAYQNT DITQLKIDHN
460 470 480 490 500
PFAKGFRDNY DSMYTASEND RLTPSPTDSP RSHQIVPGGR YGVQNFFPEP
510 520 530 540 550
FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL VTPVQQPVTN
560 570 580 590 600
KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG
610 620 630 640 650
AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD
660 670 680 690 700
SSDSGVYNSA CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY

YAFYTSP
Length:707
Mass (Da):74,801
Last modified:March 23, 2010 - v3
Checksum:i277AA462E214A927
GO
Isoform 2 (identifier: O54839-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     463-481: Missing.

Show »
Length:688
Mass (Da):72,681
Checksum:i59CB0B89E920BFE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144E → D in BAC40968 (PubMed:16141072).Curated1
Sequence conflicti159A → T in BAC40968 (PubMed:16141072).Curated1
Sequence conflicti178V → G in BAA83416 (PubMed:10407135).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038806463 – 481Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031037 mRNA. Translation: BAA83416.1.
AB032373 Genomic DNA. Translation: BAB07808.1.
AK089817 mRNA. Translation: BAC40968.1.
AK143454 mRNA. Translation: BAE25384.1.
AC173340 Genomic DNA. No translation available.
BC094319 mRNA. Translation: AAH94319.1.
AF013281 mRNA. Translation: AAC16233.1.
CCDSiCCDS23603.1. [O54839-1]
CCDS52960.1. [O54839-2]
RefSeqiNP_001158261.1. NM_001164789.1. [O54839-2]
NP_034266.2. NM_010136.3. [O54839-1]
UniGeneiMm.200692.

Genome annotation databases

EnsembliENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. [O54839-1]
ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446. [O54839-2]
GeneIDi13813.
KEGGimmu:13813.
UCSCiuc009rzo.2. mouse. [O54839-1]
uc009rzp.2. mouse. [O54839-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031037 mRNA. Translation: BAA83416.1.
AB032373 Genomic DNA. Translation: BAB07808.1.
AK089817 mRNA. Translation: BAC40968.1.
AK143454 mRNA. Translation: BAE25384.1.
AC173340 Genomic DNA. No translation available.
BC094319 mRNA. Translation: AAH94319.1.
AF013281 mRNA. Translation: AAC16233.1.
CCDSiCCDS23603.1. [O54839-1]
CCDS52960.1. [O54839-2]
RefSeqiNP_001158261.1. NM_001164789.1. [O54839-2]
NP_034266.2. NM_010136.3. [O54839-1]
UniGeneiMm.200692.

3D structure databases

ProteinModelPortaliO54839.
SMRiO54839.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199457. 2 interactors.
STRINGi10090.ENSMUSP00000035020.

PTM databases

iPTMnetiO54839.
PhosphoSitePlusiO54839.

Proteomic databases

PaxDbiO54839.
PeptideAtlasiO54839.
PRIDEiO54839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. [O54839-1]
ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446. [O54839-2]
GeneIDi13813.
KEGGimmu:13813.
UCSCiuc009rzo.2. mouse. [O54839-1]
uc009rzp.2. mouse. [O54839-2]

Organism-specific databases

CTDi8320.
MGIiMGI:1201683. Eomes.

Phylogenomic databases

eggNOGiKOG3585. Eukaryota.
ENOG410XSTS. LUCA.
GeneTreeiENSGT00740000115029.
HOGENOMiHOG000218633.
HOVERGENiHBG000578.
InParanoidiO54839.
KOiK10173.
OMAiSAQRWFV.
OrthoDBiEOG091G0PQQ.
PhylomeDBiO54839.
TreeFamiTF106341.

Miscellaneous databases

PROiO54839.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032446.
CleanExiMM_EOMES.
ExpressionAtlasiO54839. baseline and differential.
GenevisibleiO54839. MM.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR032385. T-box_assoc.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
PF16176. T-box_assoc. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEOMES_MOUSE
AccessioniPrimary (citable) accession number: O54839
Secondary accession number(s): Q3UPL1
, Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 23, 2010
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.