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O54839

- EOMES_MOUSE

UniProt

O54839 - EOMES_MOUSE

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Protein

Eomesodermin homolog

Gene

Eomes

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi278 – 458181T-boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: UniProtKB
  3. RNA polymerase II activating transcription factor binding Source: BHF-UCL
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  5. RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
  6. sequence-specific DNA binding Source: MGI
  7. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. blastocyst development Source: MGI
  2. cardioblast differentiation Source: MGI
  3. CD8-positive, alpha-beta T cell differentiation involved in immune response Source: UniProtKB
  4. cell differentiation Source: MGI
  5. cell differentiation involved in embryonic placenta development Source: UniProtKB
  6. cerebral cortex neuron differentiation Source: UniProtKB
  7. cerebral cortex regionalization Source: MGI
  8. endodermal cell fate specification Source: MGI
  9. endoderm formation Source: UniProtKB
  10. interferon-gamma production Source: MGI
  11. mesodermal to mesenchymal transition involved in gastrulation Source: UniProtKB
  12. mesoderm formation Source: UniProtKB
  13. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  14. olfactory bulb development Source: MGI
  15. positive regulation of cell differentiation Source: MGI
  16. positive regulation of transcription, DNA-templated Source: UniProtKB
  17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  18. regulation of gene expression Source: MGI
  19. regulation of neurogenesis Source: MGI
  20. regulation of neuron differentiation Source: MGI
  21. regulation of transcription from RNA polymerase II promoter Source: MGI
  22. skeletal muscle cell differentiation Source: MGI
  23. stem cell maintenance Source: MGI
  24. trophectodermal cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Adaptive immunity, Differentiation, Gastrulation, Immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eomesodermin homolog
Alternative name(s):
T-box brain protein 2
Short name:
T-brain-2
Short name:
TBR-2
Gene namesi
Name:Eomes
Synonyms:Tbr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1201683. Eomes.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. intracellular Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Eomesodermin homologPRO_0000184460Add
BLAST

Proteomic databases

PRIDEiO54839.

PTM databases

PhosphoSiteiO54839.

Expressioni

Tissue specificityi

Expressed in CD8+ T-cells.1 Publication

Developmental stagei

Originally expressed in the trophoectoderm of the blastocyst and later in the extraembryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain.2 Publications

Inductioni

Up-regulated in CD8+ T-cells upon activation.1 Publication

Gene expression databases

BgeeiO54839.
CleanExiMM_EOMES.
ExpressionAtlasiO54839. baseline and differential.
GenevestigatoriO54839.

Structurei

3D structure databases

ProteinModelPortaliO54839.
SMRiO54839. Positions 271-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni592 – 707116Required for transcription activationBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi27 – 266240Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG245070.
GeneTreeiENSGT00740000115029.
HOGENOMiHOG000218633.
HOVERGENiHBG000578.
InParanoidiO54839.
KOiK10173.
OMAiHGPVYPA.
OrthoDBiEOG7GFB4D.
PhylomeDBiO54839.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O54839-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA
60 70 80 90 100
PSPQRLDLDK ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG
110 120 130 140 150
STSAVAKPGP PDGRKGSPCA EEELPSAATA AATARYSMDS LSSERYYLPS
160 170 180 190 200
PGPQGSELAA PCSLFQYPAA AGAAHGPVYP ASNGARYPYG SMLPPGGFPA
210 220 230 240 250
AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP LYGPYAGTSA
260 270 280 290 300
AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP
310 320 330 340 350
FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK
360 370 380 390 400
MYVHPESPNT GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP
410 420 430 440 450
RLHIVEVTED GVEDLNEPSK TQTFTFSETQ FIAVTAYQNT DITQLKIDHN
460 470 480 490 500
PFAKGFRDNY DSMYTASEND RLTPSPTDSP RSHQIVPGGR YGVQNFFPEP
510 520 530 540 550
FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL VTPVQQPVTN
560 570 580 590 600
KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG
610 620 630 640 650
AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD
660 670 680 690 700
SSDSGVYNSA CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY

YAFYTSP
Length:707
Mass (Da):74,801
Last modified:March 23, 2010 - v3
Checksum:i277AA462E214A927
GO
Isoform 2 (identifier: O54839-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     463-481: Missing.

Show »
Length:688
Mass (Da):72,681
Checksum:i59CB0B89E920BFE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441E → D in BAC40968. (PubMed:16141072)Curated
Sequence conflicti159 – 1591A → T in BAC40968. (PubMed:16141072)Curated
Sequence conflicti178 – 1781V → G in BAA83416. (PubMed:10407135)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei463 – 48119Missing in isoform 2. 1 PublicationVSP_038806Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB031037 mRNA. Translation: BAA83416.1.
AB032373 Genomic DNA. Translation: BAB07808.1.
AK089817 mRNA. Translation: BAC40968.1.
AK143454 mRNA. Translation: BAE25384.1.
AC173340 Genomic DNA. No translation available.
BC094319 mRNA. Translation: AAH94319.1.
AF013281 mRNA. Translation: AAC16233.1.
CCDSiCCDS23603.1. [O54839-1]
CCDS52960.1. [O54839-2]
RefSeqiNP_001158261.1. NM_001164789.1. [O54839-2]
NP_034266.2. NM_010136.3. [O54839-1]
UniGeneiMm.200692.

Genome annotation databases

EnsembliENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. [O54839-1]
ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446. [O54839-2]
GeneIDi13813.
KEGGimmu:13813.
UCSCiuc009rzo.2. mouse. [O54839-1]
uc009rzp.2. mouse. [O54839-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB031037 mRNA. Translation: BAA83416.1 .
AB032373 Genomic DNA. Translation: BAB07808.1 .
AK089817 mRNA. Translation: BAC40968.1 .
AK143454 mRNA. Translation: BAE25384.1 .
AC173340 Genomic DNA. No translation available.
BC094319 mRNA. Translation: AAH94319.1 .
AF013281 mRNA. Translation: AAC16233.1 .
CCDSi CCDS23603.1. [O54839-1 ]
CCDS52960.1. [O54839-2 ]
RefSeqi NP_001158261.1. NM_001164789.1. [O54839-2 ]
NP_034266.2. NM_010136.3. [O54839-1 ]
UniGenei Mm.200692.

3D structure databases

ProteinModelPortali O54839.
SMRi O54839. Positions 271-457.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O54839.

Proteomic databases

PRIDEi O54839.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035020 ; ENSMUSP00000035020 ; ENSMUSG00000032446 . [O54839-1 ]
ENSMUST00000111763 ; ENSMUSP00000107393 ; ENSMUSG00000032446 . [O54839-2 ]
GeneIDi 13813.
KEGGi mmu:13813.
UCSCi uc009rzo.2. mouse. [O54839-1 ]
uc009rzp.2. mouse. [O54839-2 ]

Organism-specific databases

CTDi 8320.
MGIi MGI:1201683. Eomes.

Phylogenomic databases

eggNOGi NOG245070.
GeneTreei ENSGT00740000115029.
HOGENOMi HOG000218633.
HOVERGENi HBG000578.
InParanoidi O54839.
KOi K10173.
OMAi HGPVYPA.
OrthoDBi EOG7GFB4D.
PhylomeDBi O54839.
TreeFami TF106341.

Miscellaneous databases

NextBioi 284604.
PROi O54839.
SOURCEi Search...

Gene expression databases

Bgeei O54839.
CleanExi MM_EOMES.
ExpressionAtlasi O54839. baseline and differential.
Genevestigatori O54839.

Family and domain databases

Gene3Di 2.60.40.820. 1 hit.
InterProi IPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view ]
PANTHERi PTHR11267. PTHR11267. 1 hit.
Pfami PF00907. T-box. 1 hit.
[Graphical view ]
PRINTSi PR00937. TBOX.
SMARTi SM00425. TBOX. 1 hit.
[Graphical view ]
SUPFAMi SSF49417. SSF49417. 1 hit.
PROSITEi PS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel mammalian T-box-containing gene, Tbr2, expressed in mouse developing brain."
    Kimura N., Nakashima K., Ueno M., Taga T.
    Brain Res. Dev. Brain Res. 115:183-193(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1."
    Ueno M., Kimura N., Nakashima K., Saito-Ohara F., Inazawa J., Taga T.
    Gene 254:29-35(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
    Strain: 129/Sv.
    Tissue: Liver.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Olfactory bulb and Spleen.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  6. "A combined analysis of genomic and primary protein structure defines the phylogenetic relationship of new members of the T-box family."
    Wattler S., Russ A., Evans M., Nehls M.
    Genomics 48:24-33(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 278-457.
  7. Cited for: FUNCTION IN TROPHOBLAST DIFFERENTIATION, FUNCTION IN GASTRULATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
  8. Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  9. "Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse."
    Arnold S.J., Hofmann U.K., Bikoff E.K., Robertson E.J.
    Development 135:501-511(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GASTRULATION.
  10. "Tbr2 directs conversion of radial glia into basal precursors and guides neuronal amplification by indirect neurogenesis in the developing neocortex."
    Sessa A., Mao C.-A., Hadjantonakis A.-K., Klein W.H., Broccoli V.
    Neuron 60:56-69(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiEOMES_MOUSE
AccessioniPrimary (citable) accession number: O54839
Secondary accession number(s): Q3UPL1
, Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 23, 2010
Last modified: October 29, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3