O54839 (EOMES_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eomesodermin homolog

Alternative name(s):
T-box brain protein 2
Short name=T-brain-2
Short name=TBR-2

Gene names

Name:	Eomes
Synonyms:	Tbr2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in CD8+ T-cells. Ref.8
Developmental stage	Originally expressed in the trophoectoderm of the blastocyst and later in the extra-embryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain. Ref.7 Ref.10
Induction	Up-regulated in CD8+ T-cells upon activation. Ref.8
Disruption phenotype	Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects. Ref.7
Sequence similarities	Contains 1 T-box DNA-binding domain.

Ontologies

Keywords
Biological process	Adaptive immunity Differentiation Gastrulation Immunity Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CD8-positive, alpha-beta T cell differentiation involved in immune response Inferred from direct assay Ref.8. Source: UniProtKB cell differentiation involved in embryonic placenta development Inferred from mutant phenotype Ref.7. Source: UniProtKB cerebral cortex neuron differentiation Inferred from direct assay Ref.10. Source: UniProtKB endodermal cell fate specification Inferred from mutant phenotype. Source: MGI mesoderm formation Inferred from mutant phenotype Ref.7. Source: UniProtKB mesodermal to mesenchymal transition involved in gastrulation Inferred from mutant phenotype Ref.9. Source: UniProtKB positive regulation of cell differentiation Inferred from direct assay. Source: MGI stem cell maintenance Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW trophectodermal cell differentiation Inferred from direct assay. Source: MGI
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	RNA polymerase II regulatory region sequence-specific DNA binding Inferred from direct assay. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O54839-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O54839-2)

The sequence of this isoform differs from the canonical sequence as follows:
     463-481: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Eomesodermin homolog		PRO_0000184460
Regions
	DNA binding	278 – 458	181	T-box
	Region	592 – 707	116	Required for transcription activation By similarity
	Compositional bias	27 – 266	240	Gly-rich
Natural variations
	Alternative sequence	463 – 481	19	Missing in isoform 2.		VSP_038806
Experimental info
	Sequence conflict	144	1	E → D in BAC40968. Ref.3
	Sequence conflict	159	1	A → T in BAC40968. Ref.3
	Sequence conflict	178	1	V → G in BAA83416. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 23, 2010. Version 3. Checksum: 277AA462E214A927	FASTA	707	74,801
	10 20 30 40 50 60 MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA PSPQRLDLDK 70 80 90 100 110 120 ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG STSAVAKPGP PDGRKGSPCA 130 140 150 160 170 180 EEELPSAATA AATARYSMDS LSSERYYLPS PGPQGSELAA PCSLFQYPAA AGAAHGPVYP 190 200 210 220 230 240 ASNGARYPYG SMLPPGGFPA AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP 250 260 270 280 290 300 LYGPYAGTSA AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP 310 320 330 340 350 360 FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK MYVHPESPNT 370 380 390 400 410 420 GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP RLHIVEVTED GVEDLNEPSK 430 440 450 460 470 480 TQTFTFSETQ FIAVTAYQNT DITQLKIDHN PFAKGFRDNY DSMYTASEND RLTPSPTDSP 490 500 510 520 530 540 RSHQIVPGGR YGVQNFFPEP FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL 550 560 570 580 590 600 VTPVQQPVTN KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG 610 620 630 640 650 660 AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD SSDSGVYNSA 670 680 690 700 CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY YAFYTSP
	Isoform 2 [UniParc]. Checksum: 59CB0B89E920BFE6	FASTA	688	72,681
	10 20 30 40 50 60 MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA PSPQRLDLDK 70 80 90 100 110 120 ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG STSAVAKPGP PDGRKGSPCA 130 140 150 160 170 180 EEELPSAATA AATARYSMDS LSSERYYLPS PGPQGSELAA PCSLFQYPAA AGAAHGPVYP 190 200 210 220 230 240 ASNGARYPYG SMLPPGGFPA AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP 250 260 270 280 290 300 LYGPYAGTSA AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP 310 320 330 340 350 360 FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK MYVHPESPNT 370 380 390 400 410 420 GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP RLHIVEVTED GVEDLNEPSK 430 440 450 460 470 480 TQTFTFSETQ FIAVTAYQNT DITQLKIDHN PFAKGFRDNY DSSHQIVPGG RYGVQNFFPE 490 500 510 520 530 540 PFVNTLPQAR YYNGERTVPQ TNGLLSPQQS EEVANPPQRW LVTPVQQPVT NKLDIGSYES 550 560 570 580 590 600 EYTSSTLLPY GIKSLPLQTS HALGYYPDPT FPAMAGWGGR GAYQRKMAAG LPWTSRMSPP 610 620 630 640 650 660 VFPEDQLAKE KVKEEISSSW IETPPSIKSL DSSDSGVYNS ACKRKRLSPS TPSNGNSPPI 670 680 KCEDINTEEY SKDTSKGMGA YYAFYTSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel mammalian T-box-containing gene, Tbr2, expressed in mouse developing brain." Kimura N., Nakashima K., Ueno M., Taga T. Brain Res. Dev. Brain Res. 115:183-193(1999) [PubMed: 10407135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1." Ueno M., Kimura N., Nakashima K., Saito-Ohara F., Inazawa J., Taga T. Gene 254:29-35(2000) [PubMed: 10974533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2). Strain: 129/Sv. Tissue: Liver.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Olfactory bulb and Spleen.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[6]	"A combined analysis of genomic and primary protein structure defines the phylogenetic relationship of new members of the T-box family." Wattler S., Russ A., Evans M., Nehls M. Genomics 48:24-33(1998) [PubMed: 9503012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 278-457.
[7]	"Eomesodermin is required for mouse trophoblast development and mesoderm formation." Russ A.P., Wattler S., Colledge W.H., Aparicio S.A.J.R., Carlton M.B.L., Pearce J.J., Barton S.C., Surani M.A., Ryan K., Nehls M.C., Wilson V., Evans M.J. Nature 404:95-99(2000) [PubMed: 10716450] [Abstract] Cited for: FUNCTION IN TROPHOBLAST DIFFERENTIATION, FUNCTION IN GASTRULATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[8]	"Control of effector CD8+ T cell function by the transcription factor Eomesodermin." Pearce E.L., Mullen A.C., Martins G.A., Krawczyk C.M., Hutchins A.S., Zediak V.P., Banica M., DiCioccio C.B., Gross D.A., Mao C.-A., Shen H., Cereb N., Yang S.Y., Lindsten T., Rossant J., Hunter C.A., Reiner S.L. Science 302:1041-1043(2003) [PubMed: 14605368] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[9]	"Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse." Arnold S.J., Hofmann U.K., Bikoff E.K., Robertson E.J. Development 135:501-511(2008) [PubMed: 18171685] [Abstract] Cited for: FUNCTION IN GASTRULATION.
[10]	"Tbr2 directs conversion of radial glia into basal precursors and guides neuronal amplification by indirect neurogenesis in the developing neocortex." Sessa A., Mao C.-A., Hadjantonakis A.-K., Klein W.H., Broccoli V. Neuron 60:56-69(2008) [PubMed: 18940588] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB031037 mRNA. Translation: BAA83416.1. AB032373 Genomic DNA. Translation: BAB07808.1. AK089817 mRNA. Translation: BAC40968.1. AK143454 mRNA. Translation: BAE25384.1. AC173340 Genomic DNA. No translation available. BC094319 mRNA. Translation: AAH94319.1. AF013281 mRNA. Translation: AAC16233.1.
IPI	IPI00121983. IPI00830520.
RefSeq	NP_001158261.1. NM_001164789.1. NP_034266.2. NM_010136.3.
UniGene	Mm.200692.
3D structure databases
ProteinModelPortal	O54839.
SMR	O54839. Positions 269-458.
ModBase	Search...
Protein-protein interaction databases
STRING	O54839.
PTM databases
PhosphoSite	O54839.
Proteomic databases
PRIDE	O54839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446.
GeneID	13813.
KEGG	mmu:13813.
UCSC	uc009rzo.2. mouse. uc009rzq.2. mouse.
Organism-specific databases
CTD	8320.
MGI	MGI:1201683. Eomes.
Phylogenomic databases
HOVERGEN	HBG000578.
OMA	GFRAHVY.
OrthoDB	EOG45QHD6.
Gene expression databases
ArrayExpress	O54839.
Bgee	O54839.
CleanEx	MM_EOMES.
Genevestigator	O54839.
GermOnline	ENSMUSG00000032446. Mus musculus.
Family and domain databases
InterPro	IPR008967. p53-like_TF_DNA-bd. IPR001699. TF_T-box. IPR018186. TF_T-box_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.820. TF_T-box. 1 hit.
KO	K10173.
PANTHER	PTHR11267. TF_T-box. 1 hit.
Pfam	PF00907. T-box. 1 hit. [Graphical view]
PRINTS	PR00937. TBOX.
SMART	SM00425. TBOX. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS01283. TBOX_1. 1 hit. PS01264. TBOX_2. 1 hit. PS50252. TBOX_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

EOMES_MOUSE

Accession

Primary (citable) accession number: O54839
Secondary accession number(s): Q3UPL1 , Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	March 23, 2010
Last modified:	January 25, 2012
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program