Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O54839

- EOMES_MOUSE

UniProt

O54839 - EOMES_MOUSE

Protein

Eomesodermin homolog

Gene

Eomes

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 3 (23 Mar 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi278 – 458181T-boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB
    3. RNA polymerase II activating transcription factor binding Source: BHF-UCL
    4. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
    5. RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
    6. sequence-specific DNA binding Source: MGI
    7. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. blastocyst development Source: MGI
    2. cardioblast differentiation Source: MGI
    3. CD8-positive, alpha-beta T cell differentiation involved in immune response Source: UniProtKB
    4. cell differentiation Source: MGI
    5. cell differentiation involved in embryonic placenta development Source: UniProtKB
    6. cerebral cortex neuron differentiation Source: UniProtKB
    7. cerebral cortex regionalization Source: MGI
    8. endodermal cell fate specification Source: MGI
    9. endoderm formation Source: UniProtKB
    10. interferon-gamma production Source: MGI
    11. mesodermal to mesenchymal transition involved in gastrulation Source: UniProtKB
    12. mesoderm formation Source: UniProtKB
    13. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    14. olfactory bulb development Source: MGI
    15. positive regulation of cell differentiation Source: MGI
    16. positive regulation of transcription, DNA-templated Source: UniProtKB
    17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    18. regulation of gene expression Source: MGI
    19. regulation of neurogenesis Source: MGI
    20. regulation of neuron differentiation Source: MGI
    21. regulation of transcription from RNA polymerase II promoter Source: MGI
    22. skeletal muscle cell differentiation Source: MGI
    23. stem cell maintenance Source: MGI
    24. trophectodermal cell differentiation Source: MGI

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Adaptive immunity, Differentiation, Gastrulation, Immunity, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eomesodermin homolog
    Alternative name(s):
    T-box brain protein 2
    Short name:
    T-brain-2
    Short name:
    TBR-2
    Gene namesi
    Name:Eomes
    Synonyms:Tbr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1201683. Eomes.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. intracellular Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Eomesodermin homologPRO_0000184460Add
    BLAST

    Proteomic databases

    PRIDEiO54839.

    PTM databases

    PhosphoSiteiO54839.

    Expressioni

    Tissue specificityi

    Expressed in CD8+ T-cells.1 Publication

    Developmental stagei

    Originally expressed in the trophoectoderm of the blastocyst and later in the extraembryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain.2 Publications

    Inductioni

    Up-regulated in CD8+ T-cells upon activation.1 Publication

    Gene expression databases

    ArrayExpressiO54839.
    BgeeiO54839.
    CleanExiMM_EOMES.
    GenevestigatoriO54839.

    Structurei

    3D structure databases

    ProteinModelPortaliO54839.
    SMRiO54839. Positions 271-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni592 – 707116Required for transcription activationBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi27 – 266240Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG245070.
    GeneTreeiENSGT00740000115029.
    HOGENOMiHOG000218633.
    HOVERGENiHBG000578.
    InParanoidiQ8BN22.
    KOiK10173.
    OMAiHGPVYPA.
    OrthoDBiEOG7GFB4D.
    PhylomeDBiO54839.
    TreeFamiTF106341.

    Family and domain databases

    Gene3Di2.60.40.820. 1 hit.
    InterProiIPR008967. p53-like_TF_DNA-bd.
    IPR001699. TF_T-box.
    IPR018186. TF_T-box_CS.
    [Graphical view]
    PANTHERiPTHR11267. PTHR11267. 1 hit.
    PfamiPF00907. T-box. 1 hit.
    [Graphical view]
    PRINTSiPR00937. TBOX.
    SMARTiSM00425. TBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF49417. SSF49417. 1 hit.
    PROSITEiPS01283. TBOX_1. 1 hit.
    PS01264. TBOX_2. 1 hit.
    PS50252. TBOX_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O54839-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA    50
    PSPQRLDLDK ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG 100
    STSAVAKPGP PDGRKGSPCA EEELPSAATA AATARYSMDS LSSERYYLPS 150
    PGPQGSELAA PCSLFQYPAA AGAAHGPVYP ASNGARYPYG SMLPPGGFPA 200
    AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP LYGPYAGTSA 250
    AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP 300
    FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK 350
    MYVHPESPNT GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP 400
    RLHIVEVTED GVEDLNEPSK TQTFTFSETQ FIAVTAYQNT DITQLKIDHN 450
    PFAKGFRDNY DSMYTASEND RLTPSPTDSP RSHQIVPGGR YGVQNFFPEP 500
    FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL VTPVQQPVTN 550
    KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG 600
    AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD 650
    SSDSGVYNSA CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY 700
    YAFYTSP 707
    Length:707
    Mass (Da):74,801
    Last modified:March 23, 2010 - v3
    Checksum:i277AA462E214A927
    GO
    Isoform 2 (identifier: O54839-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         463-481: Missing.

    Show »
    Length:688
    Mass (Da):72,681
    Checksum:i59CB0B89E920BFE6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti144 – 1441E → D in BAC40968. (PubMed:16141072)Curated
    Sequence conflicti159 – 1591A → T in BAC40968. (PubMed:16141072)Curated
    Sequence conflicti178 – 1781V → G in BAA83416. (PubMed:10407135)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei463 – 48119Missing in isoform 2. 1 PublicationVSP_038806Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB031037 mRNA. Translation: BAA83416.1.
    AB032373 Genomic DNA. Translation: BAB07808.1.
    AK089817 mRNA. Translation: BAC40968.1.
    AK143454 mRNA. Translation: BAE25384.1.
    AC173340 Genomic DNA. No translation available.
    BC094319 mRNA. Translation: AAH94319.1.
    AF013281 mRNA. Translation: AAC16233.1.
    CCDSiCCDS23603.1. [O54839-1]
    CCDS52960.1. [O54839-2]
    RefSeqiNP_001158261.1. NM_001164789.1. [O54839-2]
    NP_034266.2. NM_010136.3. [O54839-1]
    UniGeneiMm.200692.

    Genome annotation databases

    EnsembliENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446. [O54839-1]
    ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446. [O54839-2]
    GeneIDi13813.
    KEGGimmu:13813.
    UCSCiuc009rzo.2. mouse. [O54839-1]
    uc009rzp.2. mouse. [O54839-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB031037 mRNA. Translation: BAA83416.1 .
    AB032373 Genomic DNA. Translation: BAB07808.1 .
    AK089817 mRNA. Translation: BAC40968.1 .
    AK143454 mRNA. Translation: BAE25384.1 .
    AC173340 Genomic DNA. No translation available.
    BC094319 mRNA. Translation: AAH94319.1 .
    AF013281 mRNA. Translation: AAC16233.1 .
    CCDSi CCDS23603.1. [O54839-1 ]
    CCDS52960.1. [O54839-2 ]
    RefSeqi NP_001158261.1. NM_001164789.1. [O54839-2 ]
    NP_034266.2. NM_010136.3. [O54839-1 ]
    UniGenei Mm.200692.

    3D structure databases

    ProteinModelPortali O54839.
    SMRi O54839. Positions 271-457.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O54839.

    Proteomic databases

    PRIDEi O54839.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035020 ; ENSMUSP00000035020 ; ENSMUSG00000032446 . [O54839-1 ]
    ENSMUST00000111763 ; ENSMUSP00000107393 ; ENSMUSG00000032446 . [O54839-2 ]
    GeneIDi 13813.
    KEGGi mmu:13813.
    UCSCi uc009rzo.2. mouse. [O54839-1 ]
    uc009rzp.2. mouse. [O54839-2 ]

    Organism-specific databases

    CTDi 8320.
    MGIi MGI:1201683. Eomes.

    Phylogenomic databases

    eggNOGi NOG245070.
    GeneTreei ENSGT00740000115029.
    HOGENOMi HOG000218633.
    HOVERGENi HBG000578.
    InParanoidi Q8BN22.
    KOi K10173.
    OMAi HGPVYPA.
    OrthoDBi EOG7GFB4D.
    PhylomeDBi O54839.
    TreeFami TF106341.

    Miscellaneous databases

    NextBioi 284604.
    PROi O54839.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54839.
    Bgeei O54839.
    CleanExi MM_EOMES.
    Genevestigatori O54839.

    Family and domain databases

    Gene3Di 2.60.40.820. 1 hit.
    InterProi IPR008967. p53-like_TF_DNA-bd.
    IPR001699. TF_T-box.
    IPR018186. TF_T-box_CS.
    [Graphical view ]
    PANTHERi PTHR11267. PTHR11267. 1 hit.
    Pfami PF00907. T-box. 1 hit.
    [Graphical view ]
    PRINTSi PR00937. TBOX.
    SMARTi SM00425. TBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49417. SSF49417. 1 hit.
    PROSITEi PS01283. TBOX_1. 1 hit.
    PS01264. TBOX_2. 1 hit.
    PS50252. TBOX_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel mammalian T-box-containing gene, Tbr2, expressed in mouse developing brain."
      Kimura N., Nakashima K., Ueno M., Taga T.
      Brain Res. Dev. Brain Res. 115:183-193(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1."
      Ueno M., Kimura N., Nakashima K., Saito-Ohara F., Inazawa J., Taga T.
      Gene 254:29-35(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
      Strain: 129/Sv.
      Tissue: Liver.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Olfactory bulb and Spleen.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    6. "A combined analysis of genomic and primary protein structure defines the phylogenetic relationship of new members of the T-box family."
      Wattler S., Russ A., Evans M., Nehls M.
      Genomics 48:24-33(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 278-457.
    7. Cited for: FUNCTION IN TROPHOBLAST DIFFERENTIATION, FUNCTION IN GASTRULATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    8. Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    9. "Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse."
      Arnold S.J., Hofmann U.K., Bikoff E.K., Robertson E.J.
      Development 135:501-511(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GASTRULATION.
    10. "Tbr2 directs conversion of radial glia into basal precursors and guides neuronal amplification by indirect neurogenesis in the developing neocortex."
      Sessa A., Mao C.-A., Hadjantonakis A.-K., Klein W.H., Broccoli V.
      Neuron 60:56-69(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiEOMES_MOUSE
    AccessioniPrimary (citable) accession number: O54839
    Secondary accession number(s): Q3UPL1
    , Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 114 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3