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Protein

Eomesodermin homolog

Gene

Eomes

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi278 – 458T-boxPROSITE-ProRule annotationAdd BLAST181

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • blastocyst development Source: MGI
  • brain development Source: MGI
  • cardioblast differentiation Source: MGI
  • CD8-positive, alpha-beta T cell differentiation involved in immune response Source: UniProtKB
  • cell differentiation Source: MGI
  • cell differentiation involved in embryonic placenta development Source: UniProtKB
  • cerebral cortex neuron differentiation Source: UniProtKB
  • cerebral cortex regionalization Source: MGI
  • endodermal cell fate specification Source: MGI
  • endoderm formation Source: UniProtKB
  • interferon-gamma production Source: MGI
  • mesodermal to mesenchymal transition involved in gastrulation Source: UniProtKB
  • mesoderm formation Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • olfactory bulb development Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of neurogenesis Source: MGI
  • regulation of neuron differentiation Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • trophectodermal cell differentiation Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processAdaptive immunity, Differentiation, Gastrulation, Immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eomesodermin homolog
Alternative name(s):
T-box brain protein 2
Short name:
T-brain-2
Short name:
TBR-2
Gene namesi
Name:Eomes
Synonyms:Tbr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1201683 Eomes

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001844601 – 707Eomesodermin homologAdd BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineCombined sources1
Modified residuei473PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54839
PeptideAtlasiO54839
PRIDEiO54839

PTM databases

iPTMnetiO54839
PhosphoSitePlusiO54839

Expressioni

Tissue specificityi

Expressed in CD8+ T-cells.1 Publication

Developmental stagei

Originally expressed in the trophoectoderm of the blastocyst and later in the extraembryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain.2 Publications

Inductioni

Up-regulated in CD8+ T-cells upon activation.1 Publication

Gene expression databases

BgeeiENSMUSG00000032446
CleanExiMM_EOMES
ExpressionAtlasiO54839 baseline and differential
GenevisibleiO54839 MM

Interactioni

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi199457, 2 interactors
IntActiO54839, 1 interactor
STRINGi10090.ENSMUSP00000035020

Structurei

3D structure databases

ProteinModelPortaliO54839
SMRiO54839
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni592 – 707Required for transcription activationBy similarityAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 266Gly-richAdd BLAST240

Phylogenomic databases

eggNOGiKOG3585 Eukaryota
ENOG410XSTS LUCA
GeneTreeiENSGT00900000140847
HOGENOMiHOG000218633
HOVERGENiHBG000578
InParanoidiO54839
KOiK10173
OMAiQPFFQDQ
OrthoDBiEOG091G0PQQ
PhylomeDBiO54839
TreeFamiTF106341

Family and domain databases

CDDicd00182 TBOX, 1 hit
Gene3Di2.60.40.820, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR032385 T-box_assoc
IPR036960 T-box_sf
IPR001699 TF_T-box
IPR018186 TF_T-box_CS
PANTHERiPTHR11267 PTHR11267, 1 hit
PfamiView protein in Pfam
PF00907 T-box, 1 hit
PF16176 T-box_assoc, 1 hit
PRINTSiPR00937 TBOX
SMARTiView protein in SMART
SM00425 TBOX, 1 hit
SUPFAMiSSF49417 SSF49417, 1 hit
PROSITEiView protein in PROSITE
PS01283 TBOX_1, 1 hit
PS01264 TBOX_2, 1 hit
PS50252 TBOX_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54839-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA
60 70 80 90 100
PSPQRLDLDK ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG
110 120 130 140 150
STSAVAKPGP PDGRKGSPCA EEELPSAATA AATARYSMDS LSSERYYLPS
160 170 180 190 200
PGPQGSELAA PCSLFQYPAA AGAAHGPVYP ASNGARYPYG SMLPPGGFPA
210 220 230 240 250
AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP LYGPYAGTSA
260 270 280 290 300
AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP
310 320 330 340 350
FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK
360 370 380 390 400
MYVHPESPNT GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP
410 420 430 440 450
RLHIVEVTED GVEDLNEPSK TQTFTFSETQ FIAVTAYQNT DITQLKIDHN
460 470 480 490 500
PFAKGFRDNY DSMYTASEND RLTPSPTDSP RSHQIVPGGR YGVQNFFPEP
510 520 530 540 550
FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL VTPVQQPVTN
560 570 580 590 600
KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG
610 620 630 640 650
AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD
660 670 680 690 700
SSDSGVYNSA CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY

YAFYTSP
Length:707
Mass (Da):74,801
Last modified:March 23, 2010 - v3
Checksum:i277AA462E214A927
GO
Isoform 2 (identifier: O54839-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     463-481: Missing.

Show »
Length:688
Mass (Da):72,681
Checksum:i59CB0B89E920BFE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144E → D in BAC40968 (PubMed:16141072).Curated1
Sequence conflicti159A → T in BAC40968 (PubMed:16141072).Curated1
Sequence conflicti178V → G in BAA83416 (PubMed:10407135).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038806463 – 481Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031037 mRNA Translation: BAA83416.1
AB032373 Genomic DNA Translation: BAB07808.1
AK089817 mRNA Translation: BAC40968.1
AK143454 mRNA Translation: BAE25384.1
AC173340 Genomic DNA No translation available.
BC094319 mRNA Translation: AAH94319.1
AF013281 mRNA Translation: AAC16233.1
CCDSiCCDS23603.1 [O54839-1]
CCDS52960.1 [O54839-2]
RefSeqiNP_001158261.1, NM_001164789.1 [O54839-2]
NP_034266.2, NM_010136.3 [O54839-1]
UniGeneiMm.200692

Genome annotation databases

EnsembliENSMUST00000035020; ENSMUSP00000035020; ENSMUSG00000032446 [O54839-1]
ENSMUST00000111763; ENSMUSP00000107393; ENSMUSG00000032446 [O54839-2]
GeneIDi13813
KEGGimmu:13813
UCSCiuc009rzo.2 mouse [O54839-1]
uc009rzp.2 mouse [O54839-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEOMES_MOUSE
AccessioniPrimary (citable) accession number: O54839
Secondary accession number(s): Q3UPL1
, Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 23, 2010
Last modified: April 25, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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