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Protein

Mothers against decapentaplegic homolog 9

Gene

Smad9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD) (By similarity). Has been shown to be activated by activin type I receptor-like kinase-2 (ALK-2) which stimulates heteromerization between SMAD9 and SMAD4. ALK-2 binds TGF-beta, activin and BMP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68ZincBy similarity1
Metal bindingi113ZincBy similarity1
Metal bindingi125ZincBy similarity1
Metal bindingi130ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • Mullerian duct regression Source: RGD
  • positive regulation of cell differentiation Source: RGD
  • positive regulation of transcription, DNA-templated Source: RGD
  • response to hypoxia Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • transforming growth factor beta receptor signaling pathway Source: InterPro

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 9
Short name:
MAD homolog 9
Short name:
Mothers against DPP homolog 9
Alternative name(s):
SMAD family member 9
Short name:
SMAD 9
Short name:
Smad9
Smad8
Gene namesi
Name:Smad9
Synonyms:Madh8, Madh9, Smad8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71004. Smad9.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000908771 – 434Mothers against decapentaplegic homolog 9Add BLAST434

Post-translational modificationi

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase (By similarity). Phosphorylated by activin type I receptor-like kinase-2 (ALK-2).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54835.

PTM databases

iPTMnetiO54835.
PhosphoSitePlusiO54835.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000091.

Interactioni

Subunit structurei

Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit (By similarity). Interacts with RANBP3L (By similarity).By similarity

Protein-protein interaction databases

BioGridi250104. 1 interactor.
STRINGi10116.ENSRNOP00000000102.

Structurei

3D structure databases

ProteinModelPortaliO54835.
SMRiO54835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 140MH1PROSITE-ProRule annotationAdd BLAST125
Domaini236 – 434MH2PROSITE-ProRule annotationAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 49Poly-Lys7

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiO54835.
KOiK16791.
PhylomeDBiO54835.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiView protein in InterPro
IPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiView protein in Pfam
PF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
SMARTiView protein in SMART
SM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiView protein in PROSITE
PS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.

Sequencei

Sequence statusi: Complete.

O54835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPSTPISSL FSFTSPAVKR LLGWKQGDEE EKWAEKAVDS LVKKLKKKKG
60 70 80 90 100
AMDELERALS CPGQPSKCVT IPRSLDGRLQ VSHRKGLPHV IYCRVWRWPD
110 120 130 140 150
LQSHHELKPL ECCEFPFGSK QKEVCINPYH YRRVETPVLP PVLVPRHSEY
160 170 180 190 200
NPQLSLLAKF RSASLHSEPL MPHNATYPDS FQQSLGPAPP SSPGHVFPQS
210 220 230 240 250
PCPTSYPQSP GSPSESDSPY QHSDFRPVCY EEPLHWCSVA YYELNNRVGE
260 270 280 290 300
TFQASSRSVL IDGFTDPSNN RNRFCLGLLS NVNRNSTIEN TRRHIGKGVH
310 320 330 340 350
LYYVGGEVYA ECVSDSSIFV QSRNCNYQHG FHPATVCKIP SGCSLKVFNN
360 370 380 390 400
QLFAQLLAQL LAQSVHHGFE VVYELTKMCT IRMSFVKGWG AEYHRQDVTS
410 420 430
TPCWIEIHLH GPLQWLDKVL TQMGSPHNPI SSVS
Length:434
Mass (Da):48,992
Last modified:June 1, 1998 - v1
Checksum:iDF6D7D31046D2575
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012347 mRNA. Translation: AAC53515.1.
RefSeqiNP_620227.1. NM_138872.1.
UniGeneiRn.10862.

Genome annotation databases

GeneIDi85435.
KEGGirno:85435.
UCSCiRGD:71004. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSMAD9_RAT
AccessioniPrimary (citable) accession number: O54835
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: June 1, 1998
Last modified: May 10, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families