Reviewed, UniProtKB/Swiss-Prot O54834 (RHG06_MOUSE)

Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 6
Alternative name(s):
    Rho-type GTPase-activating protein 6
    Rho-type GTPase-activating protein RhoGAPX-1

Gene names

Name:

Arhgap6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	987 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Could regulate the interactions of signaling molecules with the actin cytoskeleton. Promotes continuous elongation of cytoplasmic processes during cell motility and simultaneous retraction of the cell body changing the cell morphology By similarity.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Expressed in retina and lung.
Sequence similarities	Contains 1 Rho-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	SH3-binding
Molecular function	GTPase activation
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	actin filament organization Ref.1 Inferred from direct assay. Source: MGI focal adhesion formation Ref.1 Inferred from direct assay. Source: MGI positive regulation of GTPase activity Ref.1 Inferred from direct assay. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	actin cytoskeleton Ref.1 Inferred from direct assay. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	Rho GTPase activator activity Ref.1 Inferred from direct assay. Source: MGI SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O54834-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O54834-2)

The sequence of this isoform differs from the canonical sequence as follows:
     639-660: SPDILQTEVSFSMGGRHSSTDS → TSSVLPAAGQACSQSPASDFTP
     661-987: Missing.

Isoform 3 (identifier: O54834-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.
     59-197: STVSRLHSPQ...SHPYVVWRSE → MGDPSYSEKPRLHYA

Isoform 4 (identifier: O54834-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-197: MSAQSLLHSV...SHPYVVWRSE → MYKIF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 987	987	Rho GTPase-activating protein 6		PRO_0000056705
Regions
	Domain	403 – 604	202	Rho-GAP
	Motif	344 – 354	11	SH3-binding
Amino acid modifications
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	665	1	Phosphoserine By similarity
	Modified residue	675	1	Phosphoserine Ref.5
	Modified residue	758	1	Phosphoserine By similarity
	Modified residue	781	1	Phosphoserine By similarity
	Modified residue	941	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 197	197	MSAQS…VWRSE → MYKIF in isoform 4.		VSP_026051
	Alternative sequence	1 – 58	58	Missing in isoform 3.		VSP_026052
	Alternative sequence	59 – 197	139	STVSR…VWRSE → MGDPSYSEKPRLHYA in isoform 3.		VSP_026053
	Alternative sequence	639 – 660	22	SPDIL…SSTDS → TSSVLPAAGQACSQSPASDF TP in isoform 2.		VSP_001643
	Alternative sequence	661 – 987	327	Missing in isoform 2.		VSP_001644
Experimental info
	Sequence conflict	58 – 63	6	GSTVSR → AHSKP in AAC53522. Ref.1
	Sequence conflict	58 – 63	6	GSTVSR → AHSKP in AAD55086. Ref.1
	Sequence conflict	111	1	K → N in AAC53522. Ref.1
	Sequence conflict	111	1	K → N in AAD55086. Ref.1
	Sequence conflict	157	1	L → V in AAC53522. Ref.1
	Sequence conflict	157	1	L → V in AAD55086. Ref.1
	Sequence conflict	262	1	K → N in AAC53522. Ref.1
	Sequence conflict	262	1	K → N in AAD55086. Ref.1
	Sequence conflict	521	1	L → P in BAE38520. Ref.2
	Sequence conflict	580	1	S → P in BAE38520. Ref.2
	Sequence conflict	601 – 602	2	EA → DS in AAC53522. Ref.1
	Sequence conflict	601 – 602	2	EA → DS in AAD55086. Ref.1
	Sequence conflict	664	1	S → F in AAC53522. Ref.1
	Sequence conflict	723	1	P → S in AAC53522. Ref.1
	Sequence conflict	753	1	P → Q in BAE38520. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 3. Checksum: B621845ECF1C7EBD	FASTA	987	108,844
	10 20 30 40 50 60 MSAQSLLHSV FSCSSPASGG TASAKGFSKR KLRQTRSLDP ALIGGCGSEM GAEGGLRGST 70 80 90 100 110 120 VSRLHSPQLL AEGLGSRLAS SPRSQHLRAT RFQTPRPLCS SFSTPSTPQE KSPSGSFHFD 130 140 150 160 170 180 YEVPLSRSGL KKSMAWDLPS VLAGSGSASS RSPASILSSS GGGPNGIFSS PRRWLQQRKF 190 200 210 220 230 240 QPPPNSRSHP YVVWRSEGDF TWNSMSGRSV RLRSVPIQSL SELERARLQE VAFYQLQQDC 250 260 270 280 290 300 DLGCQITIPK DGQKRKKSLR KKLDSLGKEK NKDKEFIPQA FGMPLSQVIA NDRAYKLKQD 310 320 330 340 350 360 LQREEQKDAS SDFVSSLLPF GNKKQNKELS SSNSSLSSTS ETPNESTSPN TPEPAPRARR 370 380 390 400 410 420 RGAMSVDSIT DLDDNQSRLL EALQLSLPAE AQSKKEKARD KKLSLNPIYR QVPRLVDSCC 430 440 450 460 470 480 QHLEKHGLQT VGIFRVGSSK KRVRQLREEF DRGVDVCLEE EHSVHDVAAL LKEFLRDMPD 490 500 510 520 530 540 PLLTRELYTA FINTLLLEPE EQLGTLQLLI YLLPPCNCDT LHRLLQFLSI VARHADDNVS 550 560 570 580 590 600 KDGQEVTGNK MTSLNLATIF GPNLLHKQKS SDKEYSVQSS ARAEESTAII AVVQKMIENY 610 620 630 640 650 660 EALFMVPPDL QNEVLISLLE TDPDVVDYLL RRKASQSSSP DILQTEVSFS MGGRHSSTDS 670 680 690 700 710 720 NKASSGDISP YDNNSPVLSE RSLLAMQEDR ARGGSEKLYK VPEQYTLVGH LSSPKSKSRE 730 740 750 760 770 780 SSPGPRLGKE MSEEPFNIWG TWHSTLKSGS KDPGMTGSYG DIFESSSLRP RPCSLSQGNL 790 800 810 820 830 840 SLNWPRCQGS PTGLDSGTQV IRRTQTAATV EQCSVHLPVS RVCSTPHIQD GSRGTRRPAA 850 860 870 880 890 900 SSDPFLSLNS TEDLAEGKED VAWLQSQARP VYQRPQESGK DDRRPPPPYP GSGKPATTSA 910 920 930 940 950 960 QLPLEPPLWR LQRHEEGSET AVEGGQQASG EHQTRPKKLS SAYSLSASEQ DKQNLGEASW 970 980 LDWQRERWQI WELLSTDNPD ALPETLV
	Isoform 2. Checksum: 7DB2D964FEF9A5F9	FASTA	660	72,713
	10 20 30 40 50 60 MSAQSLLHSV FSCSSPASGG TASAKGFSKR KLRQTRSLDP ALIGGCGSEM GAEGGLRGST 70 80 90 100 110 120 VSRLHSPQLL AEGLGSRLAS SPRSQHLRAT RFQTPRPLCS SFSTPSTPQE KSPSGSFHFD 130 140 150 160 170 180 YEVPLSRSGL KKSMAWDLPS VLAGSGSASS RSPASILSSS GGGPNGIFSS PRRWLQQRKF 190 200 210 220 230 240 QPPPNSRSHP YVVWRSEGDF TWNSMSGRSV RLRSVPIQSL SELERARLQE VAFYQLQQDC 250 260 270 280 290 300 DLGCQITIPK DGQKRKKSLR KKLDSLGKEK NKDKEFIPQA FGMPLSQVIA NDRAYKLKQD 310 320 330 340 350 360 LQREEQKDAS SDFVSSLLPF GNKKQNKELS SSNSSLSSTS ETPNESTSPN TPEPAPRARR 370 380 390 400 410 420 RGAMSVDSIT DLDDNQSRLL EALQLSLPAE AQSKKEKARD KKLSLNPIYR QVPRLVDSCC 430 440 450 460 470 480 QHLEKHGLQT VGIFRVGSSK KRVRQLREEF DRGVDVCLEE EHSVHDVAAL LKEFLRDMPD 490 500 510 520 530 540 PLLTRELYTA FINTLLLEPE EQLGTLQLLI YLLPPCNCDT LHRLLQFLSI VARHADDNVS 550 560 570 580 590 600 KDGQEVTGNK MTSLNLATIF GPNLLHKQKS SDKEYSVQSS ARAEESTAII AVVQKMIENY 610 620 630 640 650 660 EALFMVPPDL QNEVLISLLE TDPDVVDYLL RRKASQSSTS SVLPAAGQAC SQSPASDFTP
	Isoform 3. Checksum: 31C024E718236D05	FASTA	805	89,722
	10 20 30 40 50 60 MGDPSYSEKP RLHYAGDFTW NSMSGRSVRL RSVPIQSLSE LERARLQEVA FYQLQQDCDL 70 80 90 100 110 120 GCQITIPKDG QKRKKSLRKK LDSLGKEKNK DKEFIPQAFG MPLSQVIAND RAYKLKQDLQ 130 140 150 160 170 180 REEQKDASSD FVSSLLPFGN KKQNKELSSS NSSLSSTSET PNESTSPNTP EPAPRARRRG 190 200 210 220 230 240 AMSVDSITDL DDNQSRLLEA LQLSLPAEAQ SKKEKARDKK LSLNPIYRQV PRLVDSCCQH 250 260 270 280 290 300 LEKHGLQTVG IFRVGSSKKR VRQLREEFDR GVDVCLEEEH SVHDVAALLK EFLRDMPDPL 310 320 330 340 350 360 LTRELYTAFI NTLLLEPEEQ LGTLQLLIYL LPPCNCDTLH RLLQFLSIVA RHADDNVSKD 370 380 390 400 410 420 GQEVTGNKMT SLNLATIFGP NLLHKQKSSD KEYSVQSSAR AEESTAIIAV VQKMIENYEA 430 440 450 460 470 480 LFMVPPDLQN EVLISLLETD PDVVDYLLRR KASQSSSPDI LQTEVSFSMG GRHSSTDSNK 490 500 510 520 530 540 ASSGDISPYD NNSPVLSERS LLAMQEDRAR GGSEKLYKVP EQYTLVGHLS SPKSKSRESS 550 560 570 580 590 600 PGPRLGKEMS EEPFNIWGTW HSTLKSGSKD PGMTGSYGDI FESSSLRPRP CSLSQGNLSL 610 620 630 640 650 660 NWPRCQGSPT GLDSGTQVIR RTQTAATVEQ CSVHLPVSRV CSTPHIQDGS RGTRRPAASS 670 680 690 700 710 720 DPFLSLNSTE DLAEGKEDVA WLQSQARPVY QRPQESGKDD RRPPPPYPGS GKPATTSAQL 730 740 750 760 770 780 PLEPPLWRLQ RHEEGSETAV EGGQQASGEH QTRPKKLSSA YSLSASEQDK QNLGEASWLD 790 800 WQRERWQIWE LLSTDNPDAL PETLV
	Isoform 4. Checksum: 50805457C81512DB	FASTA	795	88,672
	10 20 30 40 50 60 MYKIFGDFTW NSMSGRSVRL RSVPIQSLSE LERARLQEVA FYQLQQDCDL GCQITIPKDG 70 80 90 100 110 120 QKRKKSLRKK LDSLGKEKNK DKEFIPQAFG MPLSQVIAND RAYKLKQDLQ REEQKDASSD 130 140 150 160 170 180 FVSSLLPFGN KKQNKELSSS NSSLSSTSET PNESTSPNTP EPAPRARRRG AMSVDSITDL 190 200 210 220 230 240 DDNQSRLLEA LQLSLPAEAQ SKKEKARDKK LSLNPIYRQV PRLVDSCCQH LEKHGLQTVG 250 260 270 280 290 300 IFRVGSSKKR VRQLREEFDR GVDVCLEEEH SVHDVAALLK EFLRDMPDPL LTRELYTAFI 310 320 330 340 350 360 NTLLLEPEEQ LGTLQLLIYL LPPCNCDTLH RLLQFLSIVA RHADDNVSKD GQEVTGNKMT 370 380 390 400 410 420 SLNLATIFGP NLLHKQKSSD KEYSVQSSAR AEESTAIIAV VQKMIENYEA LFMVPPDLQN 430 440 450 460 470 480 EVLISLLETD PDVVDYLLRR KASQSSSPDI LQTEVSFSMG GRHSSTDSNK ASSGDISPYD 490 500 510 520 530 540 NNSPVLSERS LLAMQEDRAR GGSEKLYKVP EQYTLVGHLS SPKSKSRESS PGPRLGKEMS 550 560 570 580 590 600 EEPFNIWGTW HSTLKSGSKD PGMTGSYGDI FESSSLRPRP CSLSQGNLSL NWPRCQGSPT 610 620 630 640 650 660 GLDSGTQVIR RTQTAATVEQ CSVHLPVSRV CSTPHIQDGS RGTRRPAASS DPFLSLNSTE 670 680 690 700 710 720 DLAEGKEDVA WLQSQARPVY QRPQESGKDD RRPPPPYPGS GKPATTSAQL PLEPPLWRLQ 730 740 750 760 770 780 RHEEGSETAV EGGQQASGEH QTRPKKLSSA YSLSASEQDK QNLGEASWLD WQRERWQIWE 790 LLSTDNPDAL PETLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional analysis of ARHGAP6, a novel GTPase-activating protein for RhoA." Prakash S.K., Paylor R., Jenna S., Lamarche-Vane N., Armstrong D.L., Xu B., Mancini M.A., Zoghbi H.Y. Hum. Mol. Genet. 9:477-488(2000) [PubMed: 10699171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SEQUENCE REVISION TO 600-601. Strain: 129/Sv.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Strain: C57BL/6J. Tissue: Head, Lung and Testis.
[3]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Cloning and characterization of a novel rho-type GTPase-activating protein gene (ARHGAP6) from the critical region for microphthalmia with linear skin defects." Schaefer L., Prakash S.K., Zoghbi H.Y. Genomics 46:268-277(1997) [PubMed: 9417914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 280-660 (ISOFORM 1). Strain: 129/Sv.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-675, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF012273 mRNA. Translation: AAC53522.2. AF177664 mRNA. Translation: AAD55086.1. AK048162 mRNA. Translation: BAC33263.1. Different initiation. AK048507 mRNA. Translation: BAC33352.1. AK077996 mRNA. Translation: BAC37093.1. Different initiation. AK166015 mRNA. Translation: BAE38520.1. AL663026, AL805974, AL831750 Genomic DNA. Translation: CAM17930.1. AL663026, AL805974, AL831750 Genomic DNA. Translation: CAM17933.1. AL663056, AL805974, AL831750 Genomic DNA. Translation: CAM18669.1. AL805974, AL663056, AL831750 Genomic DNA. Translation: CAM21305.1. AL805974, AL663026, AL831750 Genomic DNA. Translation: CAM21306.1. AL805974, AL663026, AL831750 Genomic DNA. Translation: CAM21309.1. AL831750, AL663056, AL805974 Genomic DNA. Translation: CAM22606.1. AL831750, AL663026, AL805974 Genomic DNA. Translation: CAM22607.1. AL831750, AL663026, AL805974 Genomic DNA. Translation: CAM22609.1.
IPI	IPI00118796. IPI00467336. IPI00742331. IPI00831349.
RefSeq	NP_033837.2.
UniGene	Mm.441810
3D structure databases
HSSP	HSSP built from PDB template 1F7C based on UniProtKB Q98935.
ModBase	Search...
PTM databases
PhosphoSite	O54834.
Proteomic databases
PRIDE	O54834.
Genome annotation databases
Ensembl	ENSMUSG00000031355. Mus musculus. [Contig view]
GeneID	11856.
KEGG	mmu:11856.
NMPDR	fig|10090.3.peg.22474.
Organism-specific databases
MGI	MGI:1196332. Arhgap6.
Phylogenomic databases
HOGENOM	O54834.
HOVERGEN	O54834.
OMA	O54834. GHLPSSK.
Gene expression databases
ArrayExpress	O54834.
Bgee	O54834.
CleanEx	MM_ARHGAP6.
Family and domain databases
InterPro	IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279841.
SOURCE	Search...

Entry information

Entry name

RHG06_MOUSE

Accession

Primary (citable) accession number: O54834
Secondary accession number(s): A2ABW4 , A2AC55, Q3TMC2, Q8BG83, Q8C842, Q9QZL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)