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O54833

- CSK22_MOUSE

UniProt

O54833 - CSK22_MOUSE

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Protein
Casein kinase II subunit alpha'
Gene
Csnk2a2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATP By similarity
Active sitei157 – 1571Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 549ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: MGI
  3. protein serine/threonine kinase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
  2. apoptotic process Source: UniProtKB-KW
  3. mitotic spindle checkpoint Source: UniProtKB
  4. protein phosphorylation Source: MGI
  5. regulation of cell cycle Source: MGI
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.
ReactomeiREACT_196632. Condensation of Prometaphase Chromosomes.
REACT_215461. Signal transduction by L1.
REACT_225118. WNT mediated activation of DVL.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha' (EC:2.7.11.1)
Short name:
CK II alpha'
Gene namesi
Name:Csnk2a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:88547. Csnk2a2.

Subcellular locationi

GO - Cellular componenti

  1. PcG protein complex Source: Ensembl
  2. protein kinase CK2 complex Source: MGI
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Infertile male mice with oligospermia and globozoospermia.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Casein kinase II subunit alpha'
PRO_0000085892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphotyrosine By similarity
Modified residuei18 – 181Phosphoserine By similarity
Modified residuei21 – 211Phosphoserine By similarity
Modified residuei97 – 971N6-acetyllysine By similarity
Modified residuei288 – 2881Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO54833.
PaxDbiO54833.
PRIDEiO54833.

PTM databases

PhosphoSiteiO54833.

Expressioni

Gene expression databases

ArrayExpressiO54833.
BgeeiO54833.
CleanExiMM_CSNK2A2.
GenevestigatoriO54833.

Interactioni

Subunit structurei

Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1 By similarity.

Protein-protein interaction databases

BioGridi198946. 5 interactions.
IntActiO54833. 5 interactions.
MINTiMINT-4092108.

Structurei

3D structure databases

ProteinModelPortaliO54833.
SMRiO54833. Positions 8-332.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 325286Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233021.
HOVERGENiHBG107282.
InParanoidiO54833.
KOiK03097.
OMAiPSWGNQD.
OrthoDBiEOG7QG446.
PhylomeDBiO54833.
TreeFamiTF300483.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54833-1 [UniParc]FASTAAdd to Basket

« Hide

MPGPAAGSRA RVYAEVNSLR SREYWDYEAH VPSWGNQDDY QLVRKLGRGK    50
YSEVFEAINI TNNERVVVKI LKPVKKKKIK REVKILENLR GGTNIIKLID 100
TVKDPVSKTP ALVFEYINNT DFKQLYQILT DFDIRFYMYE LLKALDYCHS 150
KGIMHRDVKP HNVMIDHQQK KLRLIDWGLA EFYHPAQEYN VRVASRYFKG 200
PELLVDYQMY DYSLDMWSLG CMLASMIFRK EPFFHGQDNY DQLVRIAKVL 250
GTDELYGYLK KYHIDLDPHF NDILGQHSRK RWENFIHSEN RHLVSPEALD 300
LLDKLLRYDH QQRLTAKEAM EHPYFYPVVK EQSQPCAENT VLSSGLTAAR 350
Length:350
Mass (Da):41,215
Last modified:June 1, 1998 - v1
Checksum:iC5FA314617627F5B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012251 mRNA. Translation: AAC53552.1.
AJ001420 mRNA. Translation: CAA04753.1.
BC057862 mRNA. Translation: AAH57862.1.
CCDSiCCDS22562.1.
RefSeqiNP_034104.1. NM_009974.3.
XP_006530703.1. XM_006530640.1.
UniGeneiMm.440348.
Mm.51136.

Genome annotation databases

EnsembliENSMUST00000056919; ENSMUSP00000055919; ENSMUSG00000046707.
GeneIDi13000.
KEGGimmu:13000.
UCSCiuc009myk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012251 mRNA. Translation: AAC53552.1 .
AJ001420 mRNA. Translation: CAA04753.1 .
BC057862 mRNA. Translation: AAH57862.1 .
CCDSi CCDS22562.1.
RefSeqi NP_034104.1. NM_009974.3.
XP_006530703.1. XM_006530640.1.
UniGenei Mm.440348.
Mm.51136.

3D structure databases

ProteinModelPortali O54833.
SMRi O54833. Positions 8-332.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198946. 5 interactions.
IntActi O54833. 5 interactions.
MINTi MINT-4092108.

Chemistry

BindingDBi O54833.
ChEMBLi CHEMBL5326.

PTM databases

PhosphoSitei O54833.

Proteomic databases

MaxQBi O54833.
PaxDbi O54833.
PRIDEi O54833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000056919 ; ENSMUSP00000055919 ; ENSMUSG00000046707 .
GeneIDi 13000.
KEGGi mmu:13000.
UCSCi uc009myk.2. mouse.

Organism-specific databases

CTDi 1459.
MGIi MGI:88547. Csnk2a2.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233021.
HOVERGENi HBG107282.
InParanoidi O54833.
KOi K03097.
OMAi PSWGNQD.
OrthoDBi EOG7QG446.
PhylomeDBi O54833.
TreeFami TF300483.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 3474.
Reactomei REACT_196632. Condensation of Prometaphase Chromosomes.
REACT_215461. Signal transduction by L1.
REACT_225118. WNT mediated activation of DVL.

Miscellaneous databases

ChiTaRSi CSNK2A2. mouse.
NextBioi 282812.
PROi O54833.
SOURCEi Search...

Gene expression databases

ArrayExpressi O54833.
Bgeei O54833.
CleanExi MM_CSNK2A2.
Genevestigatori O54833.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine protein kinase CK2 alpha': cDNA and genomic cloning and chromosomal mapping."
    Xu X., Rich E.S. Jr., Seldin D.C.
    Genomics 48:79-86(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Protein kinase CK2alpha' is induced by serum as a delayed early gene and cooperates with Ha-ras in fibroblast transformation."
    Orlandini M., Semplici F., Ferruzzi R., Meggio F., Pinna L.A., Oliviero S.
    J. Biol. Chem. 273:21291-21297(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.
  4. "Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit."
    Xu X., Toselli P.A., Russell L.D., Seldin D.C.
    Nat. Genet. 23:118-121(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCSK22_MOUSE
AccessioniPrimary (citable) accession number: O54833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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