Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O54833

- CSK22_MOUSE

UniProt

O54833 - CSK22_MOUSE

Protein

Casein kinase II subunit alpha'

Gene

Csnk2a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691ATPPROSITE-ProRule annotation
    Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. protein serine/threonine kinase activity Source: MGI

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. mitotic spindle checkpoint Source: UniProtKB
    3. protein phosphorylation Source: MGI
    4. regulation of cell cycle Source: MGI
    5. regulation of transcription, DNA-templated Source: UniProtKB-KW
    6. transcription, DNA-templated Source: UniProtKB-KW
    7. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis, Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 3474.
    ReactomeiREACT_196632. Condensation of Prometaphase Chromosomes.
    REACT_215461. Signal transduction by L1.
    REACT_225118. WNT mediated activation of DVL.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Casein kinase II subunit alpha' (EC:2.7.11.1)
    Short name:
    CK II alpha'
    Gene namesi
    Name:Csnk2a2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:88547. Csnk2a2.

    Subcellular locationi

    GO - Cellular componenti

    1. PcG protein complex Source: Ensembl
    2. protein kinase CK2 complex Source: MGI

    Pathology & Biotechi

    Disruption phenotypei

    Infertile male mice with oligospermia and globozoospermia.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Casein kinase II subunit alpha'PRO_0000085892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131PhosphotyrosineBy similarity
    Modified residuei18 – 181PhosphoserineBy similarity
    Modified residuei21 – 211PhosphoserineBy similarity
    Modified residuei97 – 971N6-acetyllysineBy similarity
    Modified residuei288 – 2881PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO54833.
    PaxDbiO54833.
    PRIDEiO54833.

    PTM databases

    PhosphoSiteiO54833.

    Expressioni

    Gene expression databases

    ArrayExpressiO54833.
    BgeeiO54833.
    CleanExiMM_CSNK2A2.
    GenevestigatoriO54833.

    Interactioni

    Subunit structurei

    Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198946. 5 interactions.
    IntActiO54833. 5 interactions.
    MINTiMINT-4092108.

    Structurei

    3D structure databases

    ProteinModelPortaliO54833.
    SMRiO54833. Positions 8-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 325286Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233021.
    HOVERGENiHBG107282.
    InParanoidiO54833.
    KOiK03097.
    OMAiPSWGNQD.
    OrthoDBiEOG7QG446.
    PhylomeDBiO54833.
    TreeFamiTF300483.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54833-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGPAAGSRA RVYAEVNSLR SREYWDYEAH VPSWGNQDDY QLVRKLGRGK    50
    YSEVFEAINI TNNERVVVKI LKPVKKKKIK REVKILENLR GGTNIIKLID 100
    TVKDPVSKTP ALVFEYINNT DFKQLYQILT DFDIRFYMYE LLKALDYCHS 150
    KGIMHRDVKP HNVMIDHQQK KLRLIDWGLA EFYHPAQEYN VRVASRYFKG 200
    PELLVDYQMY DYSLDMWSLG CMLASMIFRK EPFFHGQDNY DQLVRIAKVL 250
    GTDELYGYLK KYHIDLDPHF NDILGQHSRK RWENFIHSEN RHLVSPEALD 300
    LLDKLLRYDH QQRLTAKEAM EHPYFYPVVK EQSQPCAENT VLSSGLTAAR 350
    Length:350
    Mass (Da):41,215
    Last modified:June 1, 1998 - v1
    Checksum:iC5FA314617627F5B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012251 mRNA. Translation: AAC53552.1.
    AJ001420 mRNA. Translation: CAA04753.1.
    BC057862 mRNA. Translation: AAH57862.1.
    CCDSiCCDS22562.1.
    RefSeqiNP_034104.1. NM_009974.3.
    XP_006530703.1. XM_006530640.1.
    UniGeneiMm.440348.
    Mm.51136.

    Genome annotation databases

    EnsembliENSMUST00000056919; ENSMUSP00000055919; ENSMUSG00000046707.
    GeneIDi13000.
    KEGGimmu:13000.
    UCSCiuc009myk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012251 mRNA. Translation: AAC53552.1 .
    AJ001420 mRNA. Translation: CAA04753.1 .
    BC057862 mRNA. Translation: AAH57862.1 .
    CCDSi CCDS22562.1.
    RefSeqi NP_034104.1. NM_009974.3.
    XP_006530703.1. XM_006530640.1.
    UniGenei Mm.440348.
    Mm.51136.

    3D structure databases

    ProteinModelPortali O54833.
    SMRi O54833. Positions 8-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198946. 5 interactions.
    IntActi O54833. 5 interactions.
    MINTi MINT-4092108.

    Chemistry

    BindingDBi O54833.
    ChEMBLi CHEMBL5326.

    PTM databases

    PhosphoSitei O54833.

    Proteomic databases

    MaxQBi O54833.
    PaxDbi O54833.
    PRIDEi O54833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000056919 ; ENSMUSP00000055919 ; ENSMUSG00000046707 .
    GeneIDi 13000.
    KEGGi mmu:13000.
    UCSCi uc009myk.2. mouse.

    Organism-specific databases

    CTDi 1459.
    MGIi MGI:88547. Csnk2a2.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233021.
    HOVERGENi HBG107282.
    InParanoidi O54833.
    KOi K03097.
    OMAi PSWGNQD.
    OrthoDBi EOG7QG446.
    PhylomeDBi O54833.
    TreeFami TF300483.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 3474.
    Reactomei REACT_196632. Condensation of Prometaphase Chromosomes.
    REACT_215461. Signal transduction by L1.
    REACT_225118. WNT mediated activation of DVL.

    Miscellaneous databases

    ChiTaRSi CSNK2A2. mouse.
    NextBioi 282812.
    PROi O54833.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54833.
    Bgeei O54833.
    CleanExi MM_CSNK2A2.
    Genevestigatori O54833.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine protein kinase CK2 alpha': cDNA and genomic cloning and chromosomal mapping."
      Xu X., Rich E.S. Jr., Seldin D.C.
      Genomics 48:79-86(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Protein kinase CK2alpha' is induced by serum as a delayed early gene and cooperates with Ha-ras in fibroblast transformation."
      Orlandini M., Semplici F., Ferruzzi R., Meggio F., Pinna L.A., Oliviero S.
      J. Biol. Chem. 273:21291-21297(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: 129.
      Tissue: Mammary gland.
    4. "Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit."
      Xu X., Toselli P.A., Russell L.D., Seldin D.C.
      Nat. Genet. 23:118-121(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCSK22_MOUSE
    AccessioniPrimary (citable) accession number: O54833
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3