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Protein

Casein kinase II subunit alpha'

Gene

Csnk2a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins (By similarity).By similarity

Miscellaneous

Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei157Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: MGI
  • protein N-terminus binding Source: MGI
  • protein serine/threonine kinase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 3474
ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-201688 WNT mediated activation of DVL
R-MMU-2514853 Condensation of Prometaphase Chromosomes
R-MMU-445144 Signal transduction by L1
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding
R-MMU-8934903 Receptor Mediated Mitophagy
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8948751 Regulation of PTEN stability and activity

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha' (EC:2.7.11.1)
Short name:
CK II alpha'
Gene namesi
Name:Csnk2a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:88547 Csnk2a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Infertile male mice with oligospermia and globozoospermia.1 Publication

Chemistry databases

ChEMBLiCHEMBL5326

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858921 – 350Casein kinase II subunit alpha'Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphotyrosineBy similarity1
Modified residuei18PhosphoserineBy similarity1
Modified residuei21PhosphoserineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei288PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO54833
PaxDbiO54833
PeptideAtlasiO54833
PRIDEiO54833

PTM databases

iPTMnetiO54833
PhosphoSitePlusiO54833

Expressioni

Tissue specificityi

Highly expressed in brain, testis and mature epididymal spermatozoa. Weakly expressed in kidney, liver, lung, spleen and thymus (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000046707
CleanExiMM_CSNK2A2
ExpressionAtlasiO54833 baseline and differential
GenevisibleiO54833 MM

Interactioni

Subunit structurei

Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1 (By similarity). Interacts with CSNKA2IP (via C-terminus) (PubMed:19273531).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198946, 7 interactors
CORUMiO54833
IntActiO54833, 6 interactors
MINTiO54833
STRINGi10090.ENSMUSP00000055919

Structurei

3D structure databases

ProteinModelPortaliO54833
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 325Protein kinasePROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0668 Eukaryota
ENOG410XNPP LUCA
GeneTreeiENSGT00390000004215
HOGENOMiHOG000233021
HOVERGENiHBG107282
InParanoidiO54833
KOiK03097
OMAiNREYWDY
OrthoDBiEOG091G0AZY
PhylomeDBiO54833
TreeFamiTF300483

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O54833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGPAAGSRA RVYAEVNSLR SREYWDYEAH VPSWGNQDDY QLVRKLGRGK
60 70 80 90 100
YSEVFEAINI TNNERVVVKI LKPVKKKKIK REVKILENLR GGTNIIKLID
110 120 130 140 150
TVKDPVSKTP ALVFEYINNT DFKQLYQILT DFDIRFYMYE LLKALDYCHS
160 170 180 190 200
KGIMHRDVKP HNVMIDHQQK KLRLIDWGLA EFYHPAQEYN VRVASRYFKG
210 220 230 240 250
PELLVDYQMY DYSLDMWSLG CMLASMIFRK EPFFHGQDNY DQLVRIAKVL
260 270 280 290 300
GTDELYGYLK KYHIDLDPHF NDILGQHSRK RWENFIHSEN RHLVSPEALD
310 320 330 340 350
LLDKLLRYDH QQRLTAKEAM EHPYFYPVVK EQSQPCAENT VLSSGLTAAR
Length:350
Mass (Da):41,215
Last modified:June 1, 1998 - v1
Checksum:iC5FA314617627F5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012251 mRNA Translation: AAC53552.1
AJ001420 mRNA Translation: CAA04753.1
BC057862 mRNA Translation: AAH57862.1
CCDSiCCDS22562.1
RefSeqiNP_034104.1, NM_009974.3
UniGeneiMm.440348
Mm.51136

Genome annotation databases

EnsembliENSMUST00000056919; ENSMUSP00000055919; ENSMUSG00000046707
ENSMUST00000212214; ENSMUSP00000148404; ENSMUSG00000046707
GeneIDi13000
KEGGimmu:13000
UCSCiuc009myk.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCSK22_MOUSE
AccessioniPrimary (citable) accession number: O54833
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health