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Protein

Regulator of G-protein signaling 7

Gene

Rgs7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS7/GNB5 dimer enhances GNAO1 GTPase activity. May play a role in synaptic vesicle exocytosis. Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 7
Short name:
RGS7
Gene namesi
Name:Rgs7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1346089. Rgs7.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Interaction with PKD1 promotes location at the cell membrane. Interaction with RGS7BP promotes location at the cell membrane.By similarity

GO - Cellular componenti

  • cytosol Source: MGI
  • dendrite terminus Source: MGI
  • heterotrimeric G-protein complex Source: InterPro
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Regulator of G-protein signaling 7PRO_0000204197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei229 – 2291PhosphoserineBy similarity
Modified residuei241 – 2411PhosphoserineCombined sources
Modified residuei243 – 2431PhosphothreonineCombined sources
Modified residuei434 – 4341PhosphoserineBy similarity

Post-translational modificationi

Palmitoylated.By similarity
Ubiquitinated, leading to rapid proteasomal degradation.By similarity
Phosphorylation and subsequent interaction with 14-3-3 proteins inhibits GAP activity.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO54829.
PaxDbiO54829.
PRIDEiO54829.

PTM databases

iPTMnetiO54829.
PhosphoSiteiO54829.

Expressioni

Tissue specificityi

Detected in brain (at protein level).1 Publication

Gene expression databases

BgeeiO54829.
CleanExiMM_RGS7.
ExpressionAtlasiO54829. baseline and differential.
GenevisibleiO54829. MM.

Interactioni

Subunit structurei

Interacts with PKD1; this prevents rapid proteasomal degradation. Interacts with GNB5 (By similarity). Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with GNB5 (PubMed:15632198, PubMed:15897264). Interacts (phosphorylated form) with 14-3-3 protein YWHAQ (PubMed:10862767). Interacts with SNAPIN. Interacts with GNAI1 (By similarity). Interacts with GNAO1, GNAI3 and GNAZ (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204852. 1 interaction.
IntActiO54829. 2 interactions.
MINTiMINT-4132332.
STRINGi10090.ENSMUSP00000027812.

Structurei

3D structure databases

ProteinModelPortaliO54829.
SMRiO54829. Positions 19-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 11276DEPPROSITE-ProRule annotationAdd
BLAST
Domaini255 – 31662G protein gammaAdd
BLAST
Domaini333 – 448116RGSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 1 G protein gamma domain.Curated
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000220864.
HOVERGENiHBG007404.
InParanoidiO54829.
KOiK16449.
OrthoDBiEOG7BZVS5.
TreeFamiTF351956.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQGNNYGQT SNGVADESPN MLVYRKMEDV IARMQDEKNG IPIRTVKSFL
60 70 80 90 100
SKIPSVFSGS DIVQWLIKNL TIEDPVEALH LGTLMAAHGY FFPISDHVLT
110 120 130 140 150
LKDDGTFYRF QTPYFWPSNC WEPENTDYAV YLCKRTMQNK ARLELADYEA
160 170 180 190 200
ESLARLQRAF ARKWEFIFMQ AEAQAKVDKK RDKIERKILD SQERAFWDVH
210 220 230 240 250
RPVPGCVNTT EVDIKKSSRM RNPHKTRKSV YGLQNDIRSH SPTHTPTPET
260 270 280 290 300
KPPTEDELHQ QIKYWQIQLD RHRLKMSKVA DSLLSYTEQY VEYDPFLVPP
310 320 330 340 350
DPSNPWLSDD TTFWELEASK EPSQQRVKRW GFGMDEALKD PVGREQFLKF
360 370 380 390 400
LESEFSSENL RFWLAVEDLK RRPIREVPSR VQEIWQEFLA PGAPSAINLD
410 420 430 440 450
SKSYDKTTQN VKEPGRYTFE DAQEHIYKLM KSDSYPRFIR SSAYQELLQA
460
KRKGKTLTSK RLTSLVQSY
Length:469
Mass (Da):54,792
Last modified:July 27, 2011 - v2
Checksum:iAE4B91280D87F211
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti461 – 4611R → S in AAC99483 (PubMed:9459445).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011360 mRNA. Translation: AAC99483.1.
AC102499 Genomic DNA. No translation available.
AC113050 Genomic DNA. No translation available.
AC117693 Genomic DNA. No translation available.
AC117734 Genomic DNA. No translation available.
AC159973 Genomic DNA. No translation available.
CCDSiCCDS15546.1.
RefSeqiNP_036010.2. NM_011880.3.
UniGeneiMm.7956.

Genome annotation databases

EnsembliENSMUST00000027812; ENSMUSP00000027812; ENSMUSG00000026527.
ENSMUST00000192227; ENSMUSP00000142278; ENSMUSG00000026527.
GeneIDi24012.
KEGGimmu:24012.
UCSCiuc007dtk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011360 mRNA. Translation: AAC99483.1.
AC102499 Genomic DNA. No translation available.
AC113050 Genomic DNA. No translation available.
AC117693 Genomic DNA. No translation available.
AC117734 Genomic DNA. No translation available.
AC159973 Genomic DNA. No translation available.
CCDSiCCDS15546.1.
RefSeqiNP_036010.2. NM_011880.3.
UniGeneiMm.7956.

3D structure databases

ProteinModelPortaliO54829.
SMRiO54829. Positions 19-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204852. 1 interaction.
IntActiO54829. 2 interactions.
MINTiMINT-4132332.
STRINGi10090.ENSMUSP00000027812.

PTM databases

iPTMnetiO54829.
PhosphoSiteiO54829.

Proteomic databases

MaxQBiO54829.
PaxDbiO54829.
PRIDEiO54829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027812; ENSMUSP00000027812; ENSMUSG00000026527.
ENSMUST00000192227; ENSMUSP00000142278; ENSMUSG00000026527.
GeneIDi24012.
KEGGimmu:24012.
UCSCiuc007dtk.2. mouse.

Organism-specific databases

CTDi6000.
MGIiMGI:1346089. Rgs7.

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000220864.
HOVERGENiHBG007404.
InParanoidiO54829.
KOiK16449.
OrthoDBiEOG7BZVS5.
TreeFamiTF351956.

Miscellaneous databases

NextBioi303939.
PROiO54829.
SOURCEiSearch...

Gene expression databases

BgeeiO54829.
CleanExiMM_RGS7.
ExpressionAtlasiO54829. baseline and differential.
GenevisibleiO54829. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RGS9, a GTPase accelerator for phototransduction."
    He W., Cowan C.W., Wensel T.G.
    Neuron 20:95-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "14-3-3 interacts with regulator of G protein signaling proteins and modulates their activity."
    Benzing T., Yaffe M.B., Arnould T., Sellin L., Schermer B., Schilling B., Schreiber R., Kunzelmann K., Leparc G.G., Kim E., Walz G.
    J. Biol. Chem. 275:28167-28172(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH 14-3-3 PROTEIN YWHAQ, TISSUE SPECIFICITY.
  4. "R7BP, a novel neuronal protein interacting with RGS proteins of the R7 family."
    Martemyanov K.A., Yoo P.J., Skiba N.P., Arshavsky V.Y.
    J. Biol. Chem. 280:5133-5136(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGS7BP.
  5. "Palmitoylation regulates plasma membrane-nuclear shuttling of R7BP, a novel membrane anchor for the RGS7 family."
    Drenan R.M., Doupnik C.A., Boyle M.P., Muglia L.J., Huettner J.E., Linder M.E., Blumer K.J.
    J. Cell Biol. 169:623-633(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGS7BP.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241 AND THR-243, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRGS7_MOUSE
AccessioniPrimary (citable) accession number: O54829
Secondary accession number(s): E9QLB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.