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Protein

Regulator of G-protein signaling 9

Gene

Rgs9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.

GO - Molecular functioni

  • GTPase activator activity Source: MGI
  • signal transducer activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Keywords - Biological processi

Sensory transduction, Vision

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 9
Short name:
RGS9
Gene namesi
Name:Rgs9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1338824. Rgs9.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • heterotrimeric G-protein complex Source: InterPro
  • nucleus Source: Ensembl
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002042041 – 675Regulator of G-protein signaling 9Add BLAST675

Post-translational modificationi

Retinal isoform 1 is light-dependent phosphorylated at 'Ser-475'. Phosphorylation is decreased by light exposition. Interaction with RGS9BP is decreased when isoform 1 is phosphorylated at 'Ser-475'.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO54828.
PRIDEiO54828.

PTM databases

iPTMnetiO54828.
PhosphoSitePlusiO54828.

Expressioni

Tissue specificityi

Isoform 1 is expressed in photoreceptor outer segments. Isoform 2 is expressed in brain striatum.

Gene expression databases

BgeeiENSMUSG00000020599.
CleanExiMM_RGS9.
ExpressionAtlasiO54828. baseline and differential.
GenevisibleiO54828. MM.

Interactioni

Subunit structurei

Heterodimer with Gbeta5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5. Component of the RGS9-1-Gbeta5 complex composed of isoform 1 of RGS9 (RGS9-1), Gbeta5 (GNB5) and RGS9BP (Probable).6 Publications

Protein-protein interaction databases

BioGridi202886. 1 interactor.
DIPiDIP-46389N.
IntActiO54828. 4 interactors.
STRINGi10090.ENSMUSP00000020920.

Structurei

Secondary structure

1675
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 27Combined sources12
Turni30 – 32Combined sources3
Helixi52 – 63Combined sources12
Helixi67 – 79Combined sources13
Beta strandi82 – 88Combined sources7
Beta strandi96 – 98Combined sources3
Beta strandi100 – 103Combined sources4
Helixi106 – 108Combined sources3
Helixi118 – 130Combined sources13
Beta strandi132 – 134Combined sources3
Helixi138 – 150Combined sources13
Helixi152 – 154Combined sources3
Helixi155 – 169Combined sources15
Helixi174 – 191Combined sources18
Beta strandi207 – 209Combined sources3
Helixi219 – 234Combined sources16
Helixi240 – 254Combined sources15
Helixi255 – 257Combined sources3
Helixi259 – 262Combined sources4
Helixi269 – 272Combined sources4
Helixi276 – 280Combined sources5
Helixi290 – 295Combined sources6
Turni296 – 298Combined sources3
Helixi300 – 305Combined sources6
Helixi307 – 319Combined sources13
Helixi325 – 337Combined sources13
Beta strandi340 – 342Combined sources3
Helixi343 – 354Combined sources12
Helixi367 – 376Combined sources10
Turni382 – 385Combined sources4
Helixi386 – 398Combined sources13
Helixi400 – 405Combined sources6
Helixi408 – 416Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95A/C1-422[»]
ProteinModelPortaliO54828.
SMRiO54828.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54828.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 105DEPPROSITE-ProRule annotationAdd BLAST76
Domaini222 – 283G protein gammaAdd BLAST62
Domaini299 – 414RGSPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 1 G protein gamma domain.Curated
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000220864.
HOVERGENiHBG007404.
InParanoidiO54828.
KOiK13765.
OMAiPRMAFLQ.
OrthoDBiEOG091G05MO.
TreeFamiTF351956.

Family and domain databases

CDDicd00068. GGL. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O54828-1) [UniParc]FASTAAdd to basket
Also known as: RGS9-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTIRHQGQQY RPRMAFLQKI EALVKDMQNP ETGVRMHNQR VLVTSVPHAM
60 70 80 90 100
TGGDVLQWIT QRLWISNLEA QNLGNFIVKY GYIYPLQDPK NLILKPDSSL
110 120 130 140 150
YRFQTPYFWP TQQWPAEDTD YAIYLAKRNI KKKGILEEYE KENYDFLNKK
160 170 180 190 200
INYKWDFVIM QAKEQYRTGK ERNKADRYAL DCQEKAYWLV HRSPPGMNNV
210 220 230 240 250
LDYGLDRVTN PNEVKKQTVT AVRKEIMYYQ QALMRSTVKS SVSLGGIVKY
260 270 280 290 300
SEQFSSNDAI MSGCLPSNPW ITDDTQFWDL NAKLVEIPTK MRVERWAFNF
310 320 330 340 350
SELIRDPKGR QSFQYFLKKE FSGENLGFWE ACEDLKYGDQ SKVKEKAEEI
360 370 380 390 400
YKLFLAPGAR RWINIDGKTM DITVKGLRHP HRYVLDAAQT HIYMLMKKDS
410 420 430 440 450
YARYLKSPIY KEMLAKAIEP QETTKRSSTL PFMRRHLRSS PSPVILRQLE
460 470 480 490 500
EEEKAREAAN TVDITQPGQH LAPSPHLAVY TGTCVPPSPS SPFSPSCRSP
510 520 530 540 550
RKPFASPSRF IRRPSIAICP SPSRVALEGS SGLEPKGEAS WSGANSGPSV
560 570 580 590 600
TENREPSADH SRPQPRAPPK ARAALSLGRF LRRGCLASPV FARLSPKCPS
610 620 630 640 650
VSHGKVQPLG DMGQQLPRLK PKKVANFFQI KMEMPTDSGT CLMDSDDPRA
660 670
GESGDQTTEK EVICPWESLA EGKAG
Length:675
Mass (Da):76,978
Last modified:July 27, 2011 - v3
Checksum:i72F1652D7D59EB05
GO
Isoform 1 (identifier: O54828-2) [UniParc]FASTAAdd to basket
Also known as: RGS9-1

The sequence of this isoform differs from the canonical sequence as follows:
     467-484: PGQHLAPSPHLAVYTGTC → VMSKLDRRSQLKKELPPK
     485-675: Missing.

Show »
Length:484
Mass (Da):56,940
Checksum:i60B37878EBB4CD3C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76F → L in AAC99481 (PubMed:9459445).Curated1
Sequence conflicti76F → L in AAD20014 (PubMed:10066255).Curated1
Sequence conflicti161Q → R in AAC99481 (PubMed:9459445).Curated1
Sequence conflicti161Q → R in AAD20014 (PubMed:10066255).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005678467 – 484PGQHL…YTGTC → VMSKLDRRSQLKKELPPK in isoform 1. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_005679485 – 675Missing in isoform 1. 1 PublicationAdd BLAST191

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011358 mRNA. Translation: AAC99481.1.
AF125046 mRNA. Translation: AAD20014.1.
AL604043 Genomic DNA. Translation: CAM22971.1.
CH466558 Genomic DNA. Translation: EDL34351.1.
BC129899 mRNA. Translation: AAI29900.1.
CCDSiCCDS25576.1. [O54828-1]
CCDS48969.1. [O54828-2]
RefSeqiNP_035398.2. NM_011268.2. [O54828-1]
UniGeneiMm.38548.

Genome annotation databases

EnsembliENSMUST00000020920; ENSMUSP00000020920; ENSMUSG00000020599. [O54828-1]
ENSMUST00000106706; ENSMUSP00000102317; ENSMUSG00000020599. [O54828-2]
GeneIDi19739.
KEGGimmu:19739.
UCSCiuc007mbx.1. mouse. [O54828-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011358 mRNA. Translation: AAC99481.1.
AF125046 mRNA. Translation: AAD20014.1.
AL604043 Genomic DNA. Translation: CAM22971.1.
CH466558 Genomic DNA. Translation: EDL34351.1.
BC129899 mRNA. Translation: AAI29900.1.
CCDSiCCDS25576.1. [O54828-1]
CCDS48969.1. [O54828-2]
RefSeqiNP_035398.2. NM_011268.2. [O54828-1]
UniGeneiMm.38548.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95A/C1-422[»]
ProteinModelPortaliO54828.
SMRiO54828.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202886. 1 interactor.
DIPiDIP-46389N.
IntActiO54828. 4 interactors.
STRINGi10090.ENSMUSP00000020920.

PTM databases

iPTMnetiO54828.
PhosphoSitePlusiO54828.

Proteomic databases

PaxDbiO54828.
PRIDEiO54828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020920; ENSMUSP00000020920; ENSMUSG00000020599. [O54828-1]
ENSMUST00000106706; ENSMUSP00000102317; ENSMUSG00000020599. [O54828-2]
GeneIDi19739.
KEGGimmu:19739.
UCSCiuc007mbx.1. mouse. [O54828-1]

Organism-specific databases

CTDi8787.
MGIiMGI:1338824. Rgs9.

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000220864.
HOVERGENiHBG007404.
InParanoidiO54828.
KOiK13765.
OMAiPRMAFLQ.
OrthoDBiEOG091G05MO.
TreeFamiTF351956.

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiRgs9. mouse.
EvolutionaryTraceiO54828.
PROiO54828.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020599.
CleanExiMM_RGS9.
ExpressionAtlasiO54828. baseline and differential.
GenevisibleiO54828. MM.

Family and domain databases

CDDicd00068. GGL. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS9_MOUSE
AccessioniPrimary (citable) accession number: O54828
Secondary accession number(s): A1L352, Q9Z0S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.