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O54828 (RGS9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 9

Short name=RGS9
Gene names
Name:Rgs9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length675 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.

Subunit structure

Heterodimer with Gbeta5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5. Component of the RGS9-1-Gbeta5 complex composed of isoform 1 of RGS9 (RGS9-1), Gbeta5 (GNB5) and RGS9BP Probable. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location

Isoform 1: Membrane; Peripheral membrane protein. Note: Isoform 1 is targeted to the membrane via its interaction with RGS9BP.

Tissue specificity

Isoform 1 is expressed in photoreceptor outer segments. Isoform 2 is expressed in brain striatum.

Post-translational modification

Retinal isoform 1 is light-dependent phosphorylated at 'Ser-475'. Phosphorylation is decreased by light exposition. Interaction with RGS9BP is decreased when isoform 1 is phosphorylated at 'Ser-475'. Ref.6

Sequence similarities

Contains 1 DEP domain.

Contains 1 G protein gamma domain.

Contains 1 RGS domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: O54828-1)

Also known as: RGS9-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: O54828-2)

Also known as: RGS9-1;

The sequence of this isoform differs from the canonical sequence as follows:
     467-484: PGQHLAPSPHLAVYTGTC → VMSKLDRRSQLKKELPPK
     485-675: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 675675Regulator of G-protein signaling 9
PRO_0000204204

Regions

Domain30 – 10576DEP
Domain222 – 28362G protein gamma
Domain299 – 414116RGS

Natural variations

Alternative sequence467 – 48418PGQHL…YTGTC → VMSKLDRRSQLKKELPPK in isoform 1.
VSP_005678
Alternative sequence485 – 675191Missing in isoform 1.
VSP_005679

Experimental info

Sequence conflict761F → L in AAC99481. Ref.1
Sequence conflict761F → L in AAD20014. Ref.2
Sequence conflict1611Q → R in AAC99481. Ref.1
Sequence conflict1611Q → R in AAD20014. Ref.2

Secondary structure

.............................................................. 675
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (RGS9-2) [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 72F1652D7D59EB05

FASTA67576,978
        10         20         30         40         50         60 
MTIRHQGQQY RPRMAFLQKI EALVKDMQNP ETGVRMHNQR VLVTSVPHAM TGGDVLQWIT 

        70         80         90        100        110        120 
QRLWISNLEA QNLGNFIVKY GYIYPLQDPK NLILKPDSSL YRFQTPYFWP TQQWPAEDTD 

       130        140        150        160        170        180 
YAIYLAKRNI KKKGILEEYE KENYDFLNKK INYKWDFVIM QAKEQYRTGK ERNKADRYAL 

       190        200        210        220        230        240 
DCQEKAYWLV HRSPPGMNNV LDYGLDRVTN PNEVKKQTVT AVRKEIMYYQ QALMRSTVKS 

       250        260        270        280        290        300 
SVSLGGIVKY SEQFSSNDAI MSGCLPSNPW ITDDTQFWDL NAKLVEIPTK MRVERWAFNF 

       310        320        330        340        350        360 
SELIRDPKGR QSFQYFLKKE FSGENLGFWE ACEDLKYGDQ SKVKEKAEEI YKLFLAPGAR 

       370        380        390        400        410        420 
RWINIDGKTM DITVKGLRHP HRYVLDAAQT HIYMLMKKDS YARYLKSPIY KEMLAKAIEP 

       430        440        450        460        470        480 
QETTKRSSTL PFMRRHLRSS PSPVILRQLE EEEKAREAAN TVDITQPGQH LAPSPHLAVY 

       490        500        510        520        530        540 
TGTCVPPSPS SPFSPSCRSP RKPFASPSRF IRRPSIAICP SPSRVALEGS SGLEPKGEAS 

       550        560        570        580        590        600 
WSGANSGPSV TENREPSADH SRPQPRAPPK ARAALSLGRF LRRGCLASPV FARLSPKCPS 

       610        620        630        640        650        660 
VSHGKVQPLG DMGQQLPRLK PKKVANFFQI KMEMPTDSGT CLMDSDDPRA GESGDQTTEK 

       670 
EVICPWESLA EGKAG 

« Hide

Isoform 1 (RGS9-1) [UniParc].

Checksum: 60B37878EBB4CD3C
Show »

FASTA48456,940

References

« Hide 'large scale' references
[1]"RGS9, a GTPase accelerator for phototransduction."
He W., Cowan C.W., Wensel T.G.
Neuron 20:95-102(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning and characterization of RGS9-2: a striatal-enriched alternatively spliced product of the RGS9 gene."
Rahman Z., Gold S.J., Potenza M.N., Cowan C.W., Ni Y.G., He W., Wensel T.G., Nestler E.J.
J. Neurosci. 19:2016-2026(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Forebrain.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Phosphorylation of RGS9-1 by an endogenous protein kinase in rod outer segments."
Hu G., Jang G.F., Cowan C.W., Wensel T.G., Palczewski K.
J. Biol. Chem. 276:22287-22295(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION OF ISOFORM 1.
[7]"R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1."
Hu G., Wensel T.G.
Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9BP.
Strain: C57BL/6 X 129.
Tissue: Retina.
[8]"Identification of protein kinase C isozymes responsible for the phosphorylation of photoreceptor-specific RGS9-1 at Ser475."
Sokal I., Hu G., Liang Y., Mao M., Wensel T.G., Palczewski K.
J. Biol. Chem. 278:8316-8325(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9BP.
[9]"The DEP domain determines subcellular targeting of the GTPase activating protein RGS9 in vivo."
Martemyanov K.A., Lishko P.V., Calero N., Keresztes G., Sokolov M., Strissel K.J., Leskov I.B., Hopp J.A., Kolesnikov A.V., Chen C.-K., Lem J., Heller S., Burns M.E., Arshavsky V.Y.
J. Neurosci. 23:10175-10181(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9BP.
[10]"R7BP, a novel neuronal protein interacting with RGS proteins of the R7 family."
Martemyanov K.A., Yoo P.J., Skiba N.P., Arshavsky V.Y.
J. Biol. Chem. 280:5133-5136(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS7BP.
[11]"Palmitoylation regulates plasma membrane-nuclear shuttling of R7BP, a novel membrane anchor for the RGS7 family."
Drenan R.M., Doupnik C.A., Boyle M.P., Muglia L.J., Huettner J.E., Linder M.E., Blumer K.J.
J. Cell Biol. 169:623-633(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS7BP.
[12]"RGS expression rate-limits recovery of rod photoresponses."
Krispel C.M., Chen D., Melling N., Chen Y.-J., Martemyanov K.A., Quillinan N., Arshavsky V.Y., Wensel T.G., Chen C.-K., Burns M.E.
Neuron 51:409-416(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9BP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF011358 mRNA. Translation: AAC99481.1.
AF125046 mRNA. Translation: AAD20014.1.
AL604043 Genomic DNA. Translation: CAM22971.1.
CH466558 Genomic DNA. Translation: EDL34351.1.
BC129899 mRNA. Translation: AAI29900.1.
CCDSCCDS25576.1. [O54828-1]
CCDS48969.1. [O54828-2]
RefSeqNP_035398.2. NM_011268.2. [O54828-1]
UniGeneMm.38548.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95A/C1-422[»]
ProteinModelPortalO54828.
SMRO54828. Positions 7-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202886. 1 interaction.
DIPDIP-46389N.
IntActO54828. 4 interactions.

Proteomic databases

PaxDbO54828.
PRIDEO54828.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020920; ENSMUSP00000020920; ENSMUSG00000020599. [O54828-1]
ENSMUST00000106706; ENSMUSP00000102317; ENSMUSG00000020599. [O54828-2]
GeneID19739.
KEGGmmu:19739.
UCSCuc007mbx.1. mouse. [O54828-1]

Organism-specific databases

CTD8787.
MGIMGI:1338824. Rgs9.

Phylogenomic databases

eggNOGNOG327614.
GeneTreeENSGT00700000104259.
HOGENOMHOG000220864.
HOVERGENHBG007404.
InParanoidA1L352.
KOK13765.
OMALVKDMQN.
OrthoDBEOG7BZVS5.
TreeFamTF351956.

Gene expression databases

ArrayExpressO54828.
BgeeO54828.
CleanExMM_RGS9.
GenevestigatorO54828.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRGS9. mouse.
EvolutionaryTraceO54828.
NextBio297184.
PROO54828.
SOURCESearch...

Entry information

Entry nameRGS9_MOUSE
AccessionPrimary (citable) accession number: O54828
Secondary accession number(s): A1L352, Q9Z0S0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot