Probable phospholipid-transporting ATPase VA

Sequence

>sp|O54827|AT10A_MOUSE Probable phospholipid-transporting ATPase VA OS=Mus musculus GN=Atp10a PE=1 SV=4 MERELPAAEESASSGWRRPRRRRWEGRTRTVRSNLLPPLGTEDSTIGAPKGERLLMRGCI QHLADNRLKTTKYTLLSFLPKNLFEQFHRLANVYFVFIALLNFVPAVNAFQPGLALAPVL FILAVTAIKDLWEDYSRHRSDHEINHLGCLVFSREEKKYVNRYWKEIRVGDFVRLCCNEI IPADILLLSSSDPDGLCHIETANLDGETNLKRRQVVRGFSELVSEFNPLTFTSVIECEKP NNDLSRFRGYIMHSNGEKAGLHKENLLLRGCTIRNTEAVAGIVIYAGHETKALLNNSGPR YKRSQLERQMNCDVLWCVLLLVCISLFSAVGHGLWVRRYQEKKALFDVPESDGSSLSPAT AAVYSFFTMIIVLQVLIPISLYVSIEIVKVCQVYFINQDIELYDEETDSQLQCRALNITE DLGQIKYIFSDKTGTLTENKMVFRRCTVSGIEYSHDANAQRLARYQEADSEEEEVVSKVG TISHRGSTGSHQSIWMTHKTQSIKSHRRTGSRAEAKRASMLSKHTAFSSPMEKDITPDPK LLEKVSECDRFLAIARHQEHPLAHLSPELSDVFDFFIALTICNTVVVTSPDQPRQKVRVR FELKSPVKTIEDFLRRFTPSRLASGCSSIGNLSTSKSSHKSGSAFLPSLSQDSMLLGLEE KLGQTAPSIASNGYASQAGQEESWASECTTDQKCPGEQREQQEGELRYEAESPDEAALVY AARAYNCALVDRLHDQVSVELPHLGRLTFELLHTLGFDSIRKRMSVVIRHPLTDEINVYT KGADSVVMDLLLPCSSDDARGRHQKKIRSKTQNYLNLYAVEGLRTLCIAKRVLSKEEYAC WLQSHIEAEASVESREELLFQSAVRLETNLHLLGATGIEDRLQEGVPETIAKLRQAGLQI WVLTGDKQETAINIAYACKLLDHGEEVITLNADSQEACAALLDQCLSYVQSRNPRSTLQN SESNLSVGFSFNPVSTSTDASPSPSLVIDGRSLAYALEKSLEDKFLFLAKQCRSVLCCRS TPLQKSMVVKLVRSKLKAMTLAIGDGANDVSMIQVADVGVGISGQEGMQAVMASDFAVPR FRYLERLLIVHGHWCYSRLANMVLYFFYKNTMFVGLLFWFQFYCGFSASAMIDQWYLIFF NLLFSSLPQLVTGVLDKDVPADMLLREPQLYKSGQNMEEYRPRAFWLNMVDAAFQSLVCF FIPYLAYYDSDVDVFTWGTPVTAIALFTFLLHLGIETKTWTWLNWLACGFSTFLFFSVAL IYNTSCATCYPPSNPYWTMQTLLGDPLFYLTCLIAPIAALLPRLFFKALQGSLFPTQLQL GRQLAKKPLNKFSDPKETFAQGQPPGHSETELSERKTMGPFETLPRDCASQASQFTQQLT CSPEASGEPSAVDTNMPLRENTLLEGLGSQASGSSMPRGAISEVCPGDSKRQSTSASQTA RLSSLFHLPSFGSLNWISSLSLASGLGSVLQLSGSSLQMDKQDGEFLSNPPQPEQDLHSF QGQVTGYL

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Reviewed, UniProtKB/Swiss-Prot O54827 (AT10A_MOUSE)

Last modified November 3, 2009. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable phospholipid-transporting ATPase VA
    EC=3.6.3.1
Alternative name(s):
    ATPase class V type 10A
    P-locus fat-associated ATPase

Gene names

Name:	Atp10a
Synonyms:	Atpc5, Pfatp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + H₂O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in testis and in white adipose tissue. Also detected in fetal tissues. Ref.5
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1508

1508

Probable phospholipid-transporting ATPase VA

PRO_0000046380

Regions

Topological domain

1 – 79

Cytoplasmic Potential

Transmembrane

80 – 101

Potential

Topological domain

102 – 107

Extracellular Potential

Transmembrane

108 – 129

Potential

Topological domain

130 – 313

184

Cytoplasmic Potential

Transmembrane

314 – 335

Potential

Topological domain

336 – 366

Extracellular Potential

Transmembrane

367 – 388

Potential

Topological domain

389 – 1101

713

Cytoplasmic Potential

Transmembrane

1102 – 1122

Potential

Topological domain

1123 – 1134

Extracellular Potential

Transmembrane

1135 – 1154

Potential

Topological domain

1155 – 1184

Cytoplasmic Potential

Transmembrane

1185 – 1206

Potential

Topological domain

1207 – 1213

Extracellular Potential

Transmembrane

1214 – 1236

Potential

Topological domain

1237 – 1242

Cytoplasmic Potential

Transmembrane

1243 – 1263

Potential

Topological domain

1264 – 1281

Extracellular Potential

Transmembrane

1282 – 1306

Potential

Topological domain

1307 – 1508

202

Cytoplasmic Potential

Compositional bias

17 – 23

Poly-Arg

Compositional bias

471 – 474

Poly-Glu

Sites

Active site

431

4-aspartylphosphate intermediate By similarity

Metal binding

1045

Magnesium By similarity

Metal binding

1049

Magnesium By similarity

Amino acid modifications

Modified residue

650

Phosphoserine Ref.6

Experimental info

Sequence conflict

16 – 22

WRRPRRR → KLAAAKK Ref.2

Sequence conflict

435

T → L Ref.2

Sequence conflict

554

I → V in AAM20894. Ref.3

Sequence conflict

687

E → D in AAF09447. Ref.1

Sequence conflict

840 – 842

CWL → TRP Ref.4

Sequence conflict

978

T → A in AAM20894. Ref.3

Sequence conflict

1113

F → S in AAF09447. Ref.1

Sequence conflict

1434

T → S in AAF09447. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

O54827-1 [UniParc].

Last modified May 10, 2005. Version 4.
Checksum: B542F86C9CD80E5B
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FASTA

1,508

168,788

        10         20         30         40         50         60 
MERELPAAEE SASSGWRRPR RRRWEGRTRT VRSNLLPPLG TEDSTIGAPK GERLLMRGCI 

        70         80         90        100        110        120 
QHLADNRLKT TKYTLLSFLP KNLFEQFHRL ANVYFVFIAL LNFVPAVNAF QPGLALAPVL 

       130        140        150        160        170        180 
FILAVTAIKD LWEDYSRHRS DHEINHLGCL VFSREEKKYV NRYWKEIRVG DFVRLCCNEI 

       190        200        210        220        230        240 
IPADILLLSS SDPDGLCHIE TANLDGETNL KRRQVVRGFS ELVSEFNPLT FTSVIECEKP 

       250        260        270        280        290        300 
NNDLSRFRGY IMHSNGEKAG LHKENLLLRG CTIRNTEAVA GIVIYAGHET KALLNNSGPR 

       310        320        330        340        350        360 
YKRSQLERQM NCDVLWCVLL LVCISLFSAV GHGLWVRRYQ EKKALFDVPE SDGSSLSPAT 

       370        380        390        400        410        420 
AAVYSFFTMI IVLQVLIPIS LYVSIEIVKV CQVYFINQDI ELYDEETDSQ LQCRALNITE 

       430        440        450        460        470        480 
DLGQIKYIFS DKTGTLTENK MVFRRCTVSG IEYSHDANAQ RLARYQEADS EEEEVVSKVG 

       490        500        510        520        530        540 
TISHRGSTGS HQSIWMTHKT QSIKSHRRTG SRAEAKRASM LSKHTAFSSP MEKDITPDPK 

       550        560        570        580        590        600 
LLEKVSECDR FLAIARHQEH PLAHLSPELS DVFDFFIALT ICNTVVVTSP DQPRQKVRVR 

       610        620        630        640        650        660 
FELKSPVKTI EDFLRRFTPS RLASGCSSIG NLSTSKSSHK SGSAFLPSLS QDSMLLGLEE 

       670        680        690        700        710        720 
KLGQTAPSIA SNGYASQAGQ EESWASECTT DQKCPGEQRE QQEGELRYEA ESPDEAALVY 

       730        740        750        760        770        780 
AARAYNCALV DRLHDQVSVE LPHLGRLTFE LLHTLGFDSI RKRMSVVIRH PLTDEINVYT 

       790        800        810        820        830        840 
KGADSVVMDL LLPCSSDDAR GRHQKKIRSK TQNYLNLYAV EGLRTLCIAK RVLSKEEYAC 

       850        860        870        880        890        900 
WLQSHIEAEA SVESREELLF QSAVRLETNL HLLGATGIED RLQEGVPETI AKLRQAGLQI 

       910        920        930        940        950        960 
WVLTGDKQET AINIAYACKL LDHGEEVITL NADSQEACAA LLDQCLSYVQ SRNPRSTLQN 

       970        980        990       1000       1010       1020 
SESNLSVGFS FNPVSTSTDA SPSPSLVIDG RSLAYALEKS LEDKFLFLAK QCRSVLCCRS 

      1030       1040       1050       1060       1070       1080 
TPLQKSMVVK LVRSKLKAMT LAIGDGANDV SMIQVADVGV GISGQEGMQA VMASDFAVPR 

      1090       1100       1110       1120       1130       1140 
FRYLERLLIV HGHWCYSRLA NMVLYFFYKN TMFVGLLFWF QFYCGFSASA MIDQWYLIFF 

      1150       1160       1170       1180       1190       1200 
NLLFSSLPQL VTGVLDKDVP ADMLLREPQL YKSGQNMEEY RPRAFWLNMV DAAFQSLVCF 

      1210       1220       1230       1240       1250       1260 
FIPYLAYYDS DVDVFTWGTP VTAIALFTFL LHLGIETKTW TWLNWLACGF STFLFFSVAL 

      1270       1280       1290       1300       1310       1320 
IYNTSCATCY PPSNPYWTMQ TLLGDPLFYL TCLIAPIAAL LPRLFFKALQ GSLFPTQLQL 

      1330       1340       1350       1360       1370       1380 
GRQLAKKPLN KFSDPKETFA QGQPPGHSET ELSERKTMGP FETLPRDCAS QASQFTQQLT 

      1390       1400       1410       1420       1430       1440 
CSPEASGEPS AVDTNMPLRE NTLLEGLGSQ ASGSSMPRGA ISEVCPGDSK RQSTSASQTA 

      1450       1460       1470       1480       1490       1500 
RLSSLFHLPS FGSLNWISSL SLASGLGSVL QLSGSSLQMD KQDGEFLSNP PQPEQDLHSF 


QGQVTGYL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of putative transbilayer amphipath transporters." Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A. Physiol. Genomics 1:139-150(1999) [PubMed: 11015572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Teratocarcinoma.
[2]	"Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs." Halleck M.S., Pradhan D., Blackman C.F., Berkes C., Williamson P.L., Schlegel R.A. Genome Res. 8:354-361(1998) [PubMed: 9548971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-435.
[3]	"An aminophospholipid translocase associated with body fat and type 2 diabetes phenotypes." Dhar M., Hauser L., Johnson D. Obes. Res. 10:695-702(2002) [PubMed: 12105293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-1508.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 840-1508.
[5]	"A novel ATPase on mouse chromosome 7 is a candidate gene for increased body fat." Dhar M., Webb L.S., Smith L., Hauser L., Johnson D., West D.B. Physiol. Genomics 4:93-100(2000) [PubMed: 11074018] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-650, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF156549 mRNA. Translation: AAF09447.1. AF011337 mRNA. Translation: AAC02902.1. AF372979 Genomic DNA. Translation: AAM20894.1. BC025643 mRNA. Translation: AAH25643.1.
IPI	IPI00338618.
RefSeq	NP_033858.2.
UniGene	Mm.135129
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O54827.
PTM databases
PhosphoSite	O54827.
Proteomic databases
PRIDE	O54827.
Genome annotation databases
Ensembl	ENSMUST00000055764; ENSMUSP00000055608; ENSMUSG00000025324; Mus musculus. [Genome view]
GeneID	11982.
KEGG	mmu:11982.
Organism-specific databases
CTD	11982.
MGI	MGI:1330809. Atp10a.
Phylogenomic databases
HOVERGEN	O54827.
OMA	KETFAQG.
Enzyme and pathway databases
BRENDA	3.6.3.1. 244.
Gene expression databases
ArrayExpress	O54827.
Bgee	O54827.
CleanEx	MM_ATP10A.
Genevestigator	O54827.
GermOnline	ENSMUSG00000025324. Mus musculus.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280129.
SOURCE	Search...

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Entry information

Entry name

AT10A_MOUSE

Accession

Primary (citable) accession number: O54827
Secondary accession number(s): Q8R3B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 10, 2005
Last modified:	November 3, 2009
This is version 87 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents