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O54827

- AT10A_MOUSE

UniProt

O54827 - AT10A_MOUSE

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Protein

Probable phospholipid-transporting ATPase VA

Gene
Atp10a, Atpc5, Pfatp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules By similarity.

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 43114-aspartylphosphate intermediate By similarity
Metal bindingi1045 – 10451Magnesium By similarity
Metal bindingi1049 – 10491Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cation-transporting ATPase activity Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. phospholipid-translocating ATPase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase VA (EC:3.6.3.1)
Alternative name(s):
ATPase class V type 10A
P-locus fat-associated ATPase
P4-ATPase flippase complex alpha subunit ATP10A
Gene namesi
Name:Atp10a
Synonyms:Atpc5, Pfatp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1330809. Atp10a.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane By similarity
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei80 – 10122Helical; Reviewed predictionAdd
BLAST
Topological domaini102 – 1076Exoplasmic loop Reviewed prediction
Transmembranei108 – 12922Helical; Reviewed predictionAdd
BLAST
Topological domaini130 – 313184Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei314 – 33522Helical; Reviewed predictionAdd
BLAST
Topological domaini336 – 36631Exoplasmic loop Reviewed predictionAdd
BLAST
Transmembranei367 – 38822Helical; Reviewed predictionAdd
BLAST
Topological domaini389 – 1101713Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1102 – 112221Helical; Reviewed predictionAdd
BLAST
Topological domaini1123 – 113412Exoplasmic loop Reviewed predictionAdd
BLAST
Transmembranei1135 – 115420Helical; Reviewed predictionAdd
BLAST
Topological domaini1155 – 118430Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1185 – 120622Helical; Reviewed predictionAdd
BLAST
Topological domaini1207 – 12137Exoplasmic loop Reviewed prediction
Transmembranei1214 – 123623Helical; Reviewed predictionAdd
BLAST
Topological domaini1237 – 12426Cytoplasmic Reviewed prediction
Transmembranei1243 – 126321Helical; Reviewed predictionAdd
BLAST
Topological domaini1264 – 128118Exoplasmic loop Reviewed predictionAdd
BLAST
Transmembranei1282 – 130625Helical; Reviewed predictionAdd
BLAST
Topological domaini1307 – 1508202Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15081508Probable phospholipid-transporting ATPase VAPRO_0000046380Add
BLAST

Proteomic databases

PRIDEiO54827.

PTM databases

PhosphoSiteiO54827.

Expressioni

Tissue specificityi

Found in testis and in white adipose tissue. Also detected in fetal tissues.1 Publication

Gene expression databases

ArrayExpressiO54827.
BgeeiO54827.
CleanExiMM_ATP10A.
GenevestigatoriO54827.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit By similarity.

Structurei

3D structure databases

ProteinModelPortaliO54827.
SMRiO54827. Positions 757-1066.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 237Poly-Arg
Compositional biasi471 – 4744Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOVERGENiHBG107129.
InParanoidiO54827.
KOiK01530.
OMAiWRYQEKK.
OrthoDBiEOG76471S.
PhylomeDBiO54827.
TreeFamiTF354252.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54827-1 [UniParc]FASTAAdd to Basket

« Hide

MERELPAAEE SASSGWRRPR RRRWEGRTRT VRSNLLPPLG TEDSTIGAPK     50
GERLLMRGCI QHLADNRLKT TKYTLLSFLP KNLFEQFHRL ANVYFVFIAL 100
LNFVPAVNAF QPGLALAPVL FILAVTAIKD LWEDYSRHRS DHEINHLGCL 150
VFSREEKKYV NRYWKEIRVG DFVRLCCNEI IPADILLLSS SDPDGLCHIE 200
TANLDGETNL KRRQVVRGFS ELVSEFNPLT FTSVIECEKP NNDLSRFRGY 250
IMHSNGEKAG LHKENLLLRG CTIRNTEAVA GIVIYAGHET KALLNNSGPR 300
YKRSQLERQM NCDVLWCVLL LVCISLFSAV GHGLWVRRYQ EKKALFDVPE 350
SDGSSLSPAT AAVYSFFTMI IVLQVLIPIS LYVSIEIVKV CQVYFINQDI 400
ELYDEETDSQ LQCRALNITE DLGQIKYIFS DKTGTLTENK MVFRRCTVSG 450
IEYSHDANAQ RLARYQEADS EEEEVVSKVG TISHRGSTGS HQSIWMTHKT 500
QSIKSHRRTG SRAEAKRASM LSKHTAFSSP MEKDITPDPK LLEKVSECDR 550
FLAIARHQEH PLAHLSPELS DVFDFFIALT ICNTVVVTSP DQPRQKVRVR 600
FELKSPVKTI EDFLRRFTPS RLASGCSSIG NLSTSKSSHK SGSAFLPSLS 650
QDSMLLGLEE KLGQTAPSIA SNGYASQAGQ EESWASECTT DQKCPGEQRE 700
QQEGELRYEA ESPDEAALVY AARAYNCALV DRLHDQVSVE LPHLGRLTFE 750
LLHTLGFDSI RKRMSVVIRH PLTDEINVYT KGADSVVMDL LLPCSSDDAR 800
GRHQKKIRSK TQNYLNLYAV EGLRTLCIAK RVLSKEEYAC WLQSHIEAEA 850
SVESREELLF QSAVRLETNL HLLGATGIED RLQEGVPETI AKLRQAGLQI 900
WVLTGDKQET AINIAYACKL LDHGEEVITL NADSQEACAA LLDQCLSYVQ 950
SRNPRSTLQN SESNLSVGFS FNPVSTSTDA SPSPSLVIDG RSLAYALEKS 1000
LEDKFLFLAK QCRSVLCCRS TPLQKSMVVK LVRSKLKAMT LAIGDGANDV 1050
SMIQVADVGV GISGQEGMQA VMASDFAVPR FRYLERLLIV HGHWCYSRLA 1100
NMVLYFFYKN TMFVGLLFWF QFYCGFSASA MIDQWYLIFF NLLFSSLPQL 1150
VTGVLDKDVP ADMLLREPQL YKSGQNMEEY RPRAFWLNMV DAAFQSLVCF 1200
FIPYLAYYDS DVDVFTWGTP VTAIALFTFL LHLGIETKTW TWLNWLACGF 1250
STFLFFSVAL IYNTSCATCY PPSNPYWTMQ TLLGDPLFYL TCLIAPIAAL 1300
LPRLFFKALQ GSLFPTQLQL GRQLAKKPLN KFSDPKETFA QGQPPGHSET 1350
ELSERKTMGP FETLPRDCAS QASQFTQQLT CSPEASGEPS AVDTNMPLRE 1400
NTLLEGLGSQ ASGSSMPRGA ISEVCPGDSK RQSTSASQTA RLSSLFHLPS 1450
FGSLNWISSL SLASGLGSVL QLSGSSLQMD KQDGEFLSNP PQPEQDLHSF 1500
QGQVTGYL 1508
Length:1,508
Mass (Da):168,788
Last modified:May 10, 2005 - v4
Checksum:iB542F86C9CD80E5B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 227WRRPRRR → KLAAAKK in AAC02902. 1 Publication
Sequence conflicti435 – 4351T → L in AAC02902. 1 Publication
Sequence conflicti554 – 5541I → V in AAM20894. 1 Publication
Sequence conflicti687 – 6871E → D in AAF09447. 1 Publication
Sequence conflicti840 – 8423CWL → TRP in AAH25643. 1 Publication
Sequence conflicti978 – 9781T → A in AAM20894. 1 Publication
Sequence conflicti1113 – 11131F → S in AAF09447. 1 Publication
Sequence conflicti1434 – 14341T → S in AAF09447. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156549 mRNA. Translation: AAF09447.1.
AF011337 mRNA. Translation: AAC02902.1.
AF372979 Genomic DNA. Translation: AAM20894.1.
BC025643 mRNA. Translation: AAH25643.1.
CCDSiCCDS39972.1.
RefSeqiNP_033858.2. NM_009728.2.
XP_006540643.1. XM_006540580.1.
XP_006540644.1. XM_006540581.1.
UniGeneiMm.135129.

Genome annotation databases

EnsembliENSMUST00000168747; ENSMUSP00000129811; ENSMUSG00000025324.
GeneIDi11982.
KEGGimmu:11982.
UCSCiuc009hef.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156549 mRNA. Translation: AAF09447.1 .
AF011337 mRNA. Translation: AAC02902.1 .
AF372979 Genomic DNA. Translation: AAM20894.1 .
BC025643 mRNA. Translation: AAH25643.1 .
CCDSi CCDS39972.1.
RefSeqi NP_033858.2. NM_009728.2.
XP_006540643.1. XM_006540580.1.
XP_006540644.1. XM_006540581.1.
UniGenei Mm.135129.

3D structure databases

ProteinModelPortali O54827.
SMRi O54827. Positions 757-1066.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O54827.

Proteomic databases

PRIDEi O54827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000168747 ; ENSMUSP00000129811 ; ENSMUSG00000025324 .
GeneIDi 11982.
KEGGi mmu:11982.
UCSCi uc009hef.2. mouse.

Organism-specific databases

CTDi 57194.
MGIi MGI:1330809. Atp10a.

Phylogenomic databases

eggNOGi COG0474.
HOVERGENi HBG107129.
InParanoidi O54827.
KOi K01530.
OMAi WRYQEKK.
OrthoDBi EOG76471S.
PhylomeDBi O54827.
TreeFami TF354252.

Miscellaneous databases

NextBioi 280129.
PROi O54827.
SOURCEi Search...

Gene expression databases

ArrayExpressi O54827.
Bgeei O54827.
CleanExi MM_ATP10A.
Genevestigatori O54827.

Family and domain databases

Gene3Di 2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
PANTHERi PTHR24092. PTHR24092. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of putative transbilayer amphipath transporters."
    Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A.
    Physiol. Genomics 1:139-150(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Teratocarcinoma.
  2. "Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs."
    Halleck M.S., Pradhan D., Blackman C.F., Berkes C., Williamson P.L., Schlegel R.A.
    Genome Res. 8:354-361(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-435.
  3. "An aminophospholipid translocase associated with body fat and type 2 diabetes phenotypes."
    Dhar M., Hauser L., Johnson D.
    Obes. Res. 10:695-702(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-1508.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 840-1508.
  5. "A novel ATPase on mouse chromosome 7 is a candidate gene for increased body fat."
    Dhar M., Webb L.S., Smith L., Hauser L., Johnson D., West D.B.
    Physiol. Genomics 4:93-100(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT10A_MOUSE
AccessioniPrimary (citable) accession number: O54827
Secondary accession number(s): Q8R3B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 10, 2005
Last modified: July 9, 2014
This is version 129 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi