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O54827

- AT10A_MOUSE

UniProt

O54827 - AT10A_MOUSE

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Protein

Probable phospholipid-transporting ATPase VA

Gene

Atp10a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).By similarity

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 43114-aspartylphosphate intermediateBy similarity
Metal bindingi1045 – 10451MagnesiumBy similarity
Metal bindingi1049 – 10491MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cation-transporting ATPase activity Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. phospholipid-translocating ATPase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_250224. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase VA (EC:3.6.3.1)
Alternative name(s):
ATPase class V type 10A
P-locus fat-associated ATPase
P4-ATPase flippase complex alpha subunit ATP10A
Gene namesi
Name:Atp10a
Synonyms:Atpc5, Pfatp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1330809. Atp10a.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane By similarity
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei80 – 10122HelicalSequence AnalysisAdd
BLAST
Topological domaini102 – 1076Exoplasmic loopSequence Analysis
Transmembranei108 – 12922HelicalSequence AnalysisAdd
BLAST
Topological domaini130 – 313184CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei314 – 33522HelicalSequence AnalysisAdd
BLAST
Topological domaini336 – 36631Exoplasmic loopSequence AnalysisAdd
BLAST
Transmembranei367 – 38822HelicalSequence AnalysisAdd
BLAST
Topological domaini389 – 1101713CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1102 – 112221HelicalSequence AnalysisAdd
BLAST
Topological domaini1123 – 113412Exoplasmic loopSequence AnalysisAdd
BLAST
Transmembranei1135 – 115420HelicalSequence AnalysisAdd
BLAST
Topological domaini1155 – 118430CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1185 – 120622HelicalSequence AnalysisAdd
BLAST
Topological domaini1207 – 12137Exoplasmic loopSequence Analysis
Transmembranei1214 – 123623HelicalSequence AnalysisAdd
BLAST
Topological domaini1237 – 12426CytoplasmicSequence Analysis
Transmembranei1243 – 126321HelicalSequence AnalysisAdd
BLAST
Topological domaini1264 – 128118Exoplasmic loopSequence AnalysisAdd
BLAST
Transmembranei1282 – 130625HelicalSequence AnalysisAdd
BLAST
Topological domaini1307 – 1508202CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15081508Probable phospholipid-transporting ATPase VAPRO_0000046380Add
BLAST

Proteomic databases

PRIDEiO54827.

PTM databases

PhosphoSiteiO54827.

Expressioni

Tissue specificityi

Found in testis and in white adipose tissue. Also detected in fetal tissues.1 Publication

Gene expression databases

BgeeiO54827.
CleanExiMM_ATP10A.
ExpressionAtlasiO54827. baseline and differential.
GenevestigatoriO54827.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit.By similarity

Structurei

3D structure databases

ProteinModelPortaliO54827.
SMRiO54827. Positions 757-1066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 237Poly-Arg
Compositional biasi471 – 4744Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOVERGENiHBG107129.
InParanoidiO54827.
KOiK01530.
OMAiWRYQEKK.
OrthoDBiEOG76471S.
PhylomeDBiO54827.
TreeFamiTF354252.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54827-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERELPAAEE SASSGWRRPR RRRWEGRTRT VRSNLLPPLG TEDSTIGAPK
60 70 80 90 100
GERLLMRGCI QHLADNRLKT TKYTLLSFLP KNLFEQFHRL ANVYFVFIAL
110 120 130 140 150
LNFVPAVNAF QPGLALAPVL FILAVTAIKD LWEDYSRHRS DHEINHLGCL
160 170 180 190 200
VFSREEKKYV NRYWKEIRVG DFVRLCCNEI IPADILLLSS SDPDGLCHIE
210 220 230 240 250
TANLDGETNL KRRQVVRGFS ELVSEFNPLT FTSVIECEKP NNDLSRFRGY
260 270 280 290 300
IMHSNGEKAG LHKENLLLRG CTIRNTEAVA GIVIYAGHET KALLNNSGPR
310 320 330 340 350
YKRSQLERQM NCDVLWCVLL LVCISLFSAV GHGLWVRRYQ EKKALFDVPE
360 370 380 390 400
SDGSSLSPAT AAVYSFFTMI IVLQVLIPIS LYVSIEIVKV CQVYFINQDI
410 420 430 440 450
ELYDEETDSQ LQCRALNITE DLGQIKYIFS DKTGTLTENK MVFRRCTVSG
460 470 480 490 500
IEYSHDANAQ RLARYQEADS EEEEVVSKVG TISHRGSTGS HQSIWMTHKT
510 520 530 540 550
QSIKSHRRTG SRAEAKRASM LSKHTAFSSP MEKDITPDPK LLEKVSECDR
560 570 580 590 600
FLAIARHQEH PLAHLSPELS DVFDFFIALT ICNTVVVTSP DQPRQKVRVR
610 620 630 640 650
FELKSPVKTI EDFLRRFTPS RLASGCSSIG NLSTSKSSHK SGSAFLPSLS
660 670 680 690 700
QDSMLLGLEE KLGQTAPSIA SNGYASQAGQ EESWASECTT DQKCPGEQRE
710 720 730 740 750
QQEGELRYEA ESPDEAALVY AARAYNCALV DRLHDQVSVE LPHLGRLTFE
760 770 780 790 800
LLHTLGFDSI RKRMSVVIRH PLTDEINVYT KGADSVVMDL LLPCSSDDAR
810 820 830 840 850
GRHQKKIRSK TQNYLNLYAV EGLRTLCIAK RVLSKEEYAC WLQSHIEAEA
860 870 880 890 900
SVESREELLF QSAVRLETNL HLLGATGIED RLQEGVPETI AKLRQAGLQI
910 920 930 940 950
WVLTGDKQET AINIAYACKL LDHGEEVITL NADSQEACAA LLDQCLSYVQ
960 970 980 990 1000
SRNPRSTLQN SESNLSVGFS FNPVSTSTDA SPSPSLVIDG RSLAYALEKS
1010 1020 1030 1040 1050
LEDKFLFLAK QCRSVLCCRS TPLQKSMVVK LVRSKLKAMT LAIGDGANDV
1060 1070 1080 1090 1100
SMIQVADVGV GISGQEGMQA VMASDFAVPR FRYLERLLIV HGHWCYSRLA
1110 1120 1130 1140 1150
NMVLYFFYKN TMFVGLLFWF QFYCGFSASA MIDQWYLIFF NLLFSSLPQL
1160 1170 1180 1190 1200
VTGVLDKDVP ADMLLREPQL YKSGQNMEEY RPRAFWLNMV DAAFQSLVCF
1210 1220 1230 1240 1250
FIPYLAYYDS DVDVFTWGTP VTAIALFTFL LHLGIETKTW TWLNWLACGF
1260 1270 1280 1290 1300
STFLFFSVAL IYNTSCATCY PPSNPYWTMQ TLLGDPLFYL TCLIAPIAAL
1310 1320 1330 1340 1350
LPRLFFKALQ GSLFPTQLQL GRQLAKKPLN KFSDPKETFA QGQPPGHSET
1360 1370 1380 1390 1400
ELSERKTMGP FETLPRDCAS QASQFTQQLT CSPEASGEPS AVDTNMPLRE
1410 1420 1430 1440 1450
NTLLEGLGSQ ASGSSMPRGA ISEVCPGDSK RQSTSASQTA RLSSLFHLPS
1460 1470 1480 1490 1500
FGSLNWISSL SLASGLGSVL QLSGSSLQMD KQDGEFLSNP PQPEQDLHSF

QGQVTGYL
Length:1,508
Mass (Da):168,788
Last modified:May 10, 2005 - v4
Checksum:iB542F86C9CD80E5B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 227WRRPRRR → KLAAAKK in AAC02902. (PubMed:9548971)Curated
Sequence conflicti435 – 4351T → L in AAC02902. (PubMed:9548971)Curated
Sequence conflicti554 – 5541I → V in AAM20894. (PubMed:12105293)Curated
Sequence conflicti687 – 6871E → D in AAF09447. (PubMed:11015572)Curated
Sequence conflicti840 – 8423CWL → TRP in AAH25643. (PubMed:15489334)Curated
Sequence conflicti978 – 9781T → A in AAM20894. (PubMed:12105293)Curated
Sequence conflicti1113 – 11131F → S in AAF09447. (PubMed:11015572)Curated
Sequence conflicti1434 – 14341T → S in AAF09447. (PubMed:11015572)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156549 mRNA. Translation: AAF09447.1.
AF011337 mRNA. Translation: AAC02902.1.
AF372979 Genomic DNA. Translation: AAM20894.1.
BC025643 mRNA. Translation: AAH25643.1.
CCDSiCCDS39972.1.
RefSeqiNP_033858.2. NM_009728.2.
XP_006540643.1. XM_006540580.1.
XP_006540644.1. XM_006540581.1.
UniGeneiMm.135129.

Genome annotation databases

EnsembliENSMUST00000168747; ENSMUSP00000129811; ENSMUSG00000025324.
GeneIDi11982.
KEGGimmu:11982.
UCSCiuc009hef.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156549 mRNA. Translation: AAF09447.1 .
AF011337 mRNA. Translation: AAC02902.1 .
AF372979 Genomic DNA. Translation: AAM20894.1 .
BC025643 mRNA. Translation: AAH25643.1 .
CCDSi CCDS39972.1.
RefSeqi NP_033858.2. NM_009728.2.
XP_006540643.1. XM_006540580.1.
XP_006540644.1. XM_006540581.1.
UniGenei Mm.135129.

3D structure databases

ProteinModelPortali O54827.
SMRi O54827. Positions 757-1066.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O54827.

Proteomic databases

PRIDEi O54827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000168747 ; ENSMUSP00000129811 ; ENSMUSG00000025324 .
GeneIDi 11982.
KEGGi mmu:11982.
UCSCi uc009hef.2. mouse.

Organism-specific databases

CTDi 57194.
MGIi MGI:1330809. Atp10a.

Phylogenomic databases

eggNOGi COG0474.
HOVERGENi HBG107129.
InParanoidi O54827.
KOi K01530.
OMAi WRYQEKK.
OrthoDBi EOG76471S.
PhylomeDBi O54827.
TreeFami TF354252.

Enzyme and pathway databases

Reactomei REACT_250224. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi 280129.
PROi O54827.
SOURCEi Search...

Gene expression databases

Bgeei O54827.
CleanExi MM_ATP10A.
ExpressionAtlasi O54827. baseline and differential.
Genevestigatori O54827.

Family and domain databases

Gene3Di 2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
PANTHERi PTHR24092. PTHR24092. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of putative transbilayer amphipath transporters."
    Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A.
    Physiol. Genomics 1:139-150(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Teratocarcinoma.
  2. "Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs."
    Halleck M.S., Pradhan D., Blackman C.F., Berkes C., Williamson P.L., Schlegel R.A.
    Genome Res. 8:354-361(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-435.
  3. "An aminophospholipid translocase associated with body fat and type 2 diabetes phenotypes."
    Dhar M., Hauser L., Johnson D.
    Obes. Res. 10:695-702(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-1508.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 840-1508.
  5. "A novel ATPase on mouse chromosome 7 is a candidate gene for increased body fat."
    Dhar M., Webb L.S., Smith L., Hauser L., Johnson D., West D.B.
    Physiol. Genomics 4:93-100(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT10A_MOUSE
AccessioniPrimary (citable) accession number: O54827
Secondary accession number(s): Q8R3B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 10, 2005
Last modified: November 26, 2014
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3