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O54827

- AT10A_MOUSE

UniProt

O54827 - AT10A_MOUSE

Protein

Probable phospholipid-transporting ATPase VA

Gene

Atp10a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 4 (10 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules By similarity.By similarity

    Catalytic activityi

    ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei431 – 43114-aspartylphosphate intermediateBy similarity
    Metal bindingi1045 – 10451MagnesiumBy similarity
    Metal bindingi1049 – 10491MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cation-transporting ATPase activity Source: InterPro
    3. magnesium ion binding Source: InterPro
    4. phospholipid-translocating ATPase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable phospholipid-transporting ATPase VA (EC:3.6.3.1)
    Alternative name(s):
    ATPase class V type 10A
    P-locus fat-associated ATPase
    P4-ATPase flippase complex alpha subunit ATP10A
    Gene namesi
    Name:Atp10a
    Synonyms:Atpc5, Pfatp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1330809. Atp10a.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane By similarity
    Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15081508Probable phospholipid-transporting ATPase VAPRO_0000046380Add
    BLAST

    Proteomic databases

    PRIDEiO54827.

    PTM databases

    PhosphoSiteiO54827.

    Expressioni

    Tissue specificityi

    Found in testis and in white adipose tissue. Also detected in fetal tissues.1 Publication

    Gene expression databases

    ArrayExpressiO54827.
    BgeeiO54827.
    CleanExiMM_ATP10A.
    GenevestigatoriO54827.

    Interactioni

    Subunit structurei

    Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO54827.
    SMRiO54827. Positions 757-1066.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini102 – 1076Exoplasmic loopSequence Analysis
    Topological domaini130 – 313184CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini336 – 36631Exoplasmic loopSequence AnalysisAdd
    BLAST
    Topological domaini389 – 1101713CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1123 – 113412Exoplasmic loopSequence AnalysisAdd
    BLAST
    Topological domaini1155 – 118430CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1207 – 12137Exoplasmic loopSequence Analysis
    Topological domaini1237 – 12426CytoplasmicSequence Analysis
    Topological domaini1264 – 128118Exoplasmic loopSequence AnalysisAdd
    BLAST
    Topological domaini1307 – 1508202CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei80 – 10122HelicalSequence AnalysisAdd
    BLAST
    Transmembranei108 – 12922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei314 – 33522HelicalSequence AnalysisAdd
    BLAST
    Transmembranei367 – 38822HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1102 – 112221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1135 – 115420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1185 – 120622HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1214 – 123623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1243 – 126321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1282 – 130625HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi17 – 237Poly-Arg
    Compositional biasi471 – 4744Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOVERGENiHBG107129.
    InParanoidiO54827.
    KOiK01530.
    OMAiWRYQEKK.
    OrthoDBiEOG76471S.
    PhylomeDBiO54827.
    TreeFamiTF354252.

    Family and domain databases

    Gene3Di2.70.150.10. 2 hits.
    3.40.1110.10. 3 hits.
    3.40.50.1000. 2 hits.
    InterProiIPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PANTHERiPTHR24092. PTHR24092. 1 hit.
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54827-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERELPAAEE SASSGWRRPR RRRWEGRTRT VRSNLLPPLG TEDSTIGAPK     50
    GERLLMRGCI QHLADNRLKT TKYTLLSFLP KNLFEQFHRL ANVYFVFIAL 100
    LNFVPAVNAF QPGLALAPVL FILAVTAIKD LWEDYSRHRS DHEINHLGCL 150
    VFSREEKKYV NRYWKEIRVG DFVRLCCNEI IPADILLLSS SDPDGLCHIE 200
    TANLDGETNL KRRQVVRGFS ELVSEFNPLT FTSVIECEKP NNDLSRFRGY 250
    IMHSNGEKAG LHKENLLLRG CTIRNTEAVA GIVIYAGHET KALLNNSGPR 300
    YKRSQLERQM NCDVLWCVLL LVCISLFSAV GHGLWVRRYQ EKKALFDVPE 350
    SDGSSLSPAT AAVYSFFTMI IVLQVLIPIS LYVSIEIVKV CQVYFINQDI 400
    ELYDEETDSQ LQCRALNITE DLGQIKYIFS DKTGTLTENK MVFRRCTVSG 450
    IEYSHDANAQ RLARYQEADS EEEEVVSKVG TISHRGSTGS HQSIWMTHKT 500
    QSIKSHRRTG SRAEAKRASM LSKHTAFSSP MEKDITPDPK LLEKVSECDR 550
    FLAIARHQEH PLAHLSPELS DVFDFFIALT ICNTVVVTSP DQPRQKVRVR 600
    FELKSPVKTI EDFLRRFTPS RLASGCSSIG NLSTSKSSHK SGSAFLPSLS 650
    QDSMLLGLEE KLGQTAPSIA SNGYASQAGQ EESWASECTT DQKCPGEQRE 700
    QQEGELRYEA ESPDEAALVY AARAYNCALV DRLHDQVSVE LPHLGRLTFE 750
    LLHTLGFDSI RKRMSVVIRH PLTDEINVYT KGADSVVMDL LLPCSSDDAR 800
    GRHQKKIRSK TQNYLNLYAV EGLRTLCIAK RVLSKEEYAC WLQSHIEAEA 850
    SVESREELLF QSAVRLETNL HLLGATGIED RLQEGVPETI AKLRQAGLQI 900
    WVLTGDKQET AINIAYACKL LDHGEEVITL NADSQEACAA LLDQCLSYVQ 950
    SRNPRSTLQN SESNLSVGFS FNPVSTSTDA SPSPSLVIDG RSLAYALEKS 1000
    LEDKFLFLAK QCRSVLCCRS TPLQKSMVVK LVRSKLKAMT LAIGDGANDV 1050
    SMIQVADVGV GISGQEGMQA VMASDFAVPR FRYLERLLIV HGHWCYSRLA 1100
    NMVLYFFYKN TMFVGLLFWF QFYCGFSASA MIDQWYLIFF NLLFSSLPQL 1150
    VTGVLDKDVP ADMLLREPQL YKSGQNMEEY RPRAFWLNMV DAAFQSLVCF 1200
    FIPYLAYYDS DVDVFTWGTP VTAIALFTFL LHLGIETKTW TWLNWLACGF 1250
    STFLFFSVAL IYNTSCATCY PPSNPYWTMQ TLLGDPLFYL TCLIAPIAAL 1300
    LPRLFFKALQ GSLFPTQLQL GRQLAKKPLN KFSDPKETFA QGQPPGHSET 1350
    ELSERKTMGP FETLPRDCAS QASQFTQQLT CSPEASGEPS AVDTNMPLRE 1400
    NTLLEGLGSQ ASGSSMPRGA ISEVCPGDSK RQSTSASQTA RLSSLFHLPS 1450
    FGSLNWISSL SLASGLGSVL QLSGSSLQMD KQDGEFLSNP PQPEQDLHSF 1500
    QGQVTGYL 1508
    Length:1,508
    Mass (Da):168,788
    Last modified:May 10, 2005 - v4
    Checksum:iB542F86C9CD80E5B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 227WRRPRRR → KLAAAKK in AAC02902. (PubMed:9548971)Curated
    Sequence conflicti435 – 4351T → L in AAC02902. (PubMed:9548971)Curated
    Sequence conflicti554 – 5541I → V in AAM20894. (PubMed:12105293)Curated
    Sequence conflicti687 – 6871E → D in AAF09447. (PubMed:11015572)Curated
    Sequence conflicti840 – 8423CWL → TRP in AAH25643. (PubMed:15489334)Curated
    Sequence conflicti978 – 9781T → A in AAM20894. (PubMed:12105293)Curated
    Sequence conflicti1113 – 11131F → S in AAF09447. (PubMed:11015572)Curated
    Sequence conflicti1434 – 14341T → S in AAF09447. (PubMed:11015572)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156549 mRNA. Translation: AAF09447.1.
    AF011337 mRNA. Translation: AAC02902.1.
    AF372979 Genomic DNA. Translation: AAM20894.1.
    BC025643 mRNA. Translation: AAH25643.1.
    CCDSiCCDS39972.1.
    RefSeqiNP_033858.2. NM_009728.2.
    XP_006540643.1. XM_006540580.1.
    XP_006540644.1. XM_006540581.1.
    UniGeneiMm.135129.

    Genome annotation databases

    EnsembliENSMUST00000168747; ENSMUSP00000129811; ENSMUSG00000025324.
    GeneIDi11982.
    KEGGimmu:11982.
    UCSCiuc009hef.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156549 mRNA. Translation: AAF09447.1 .
    AF011337 mRNA. Translation: AAC02902.1 .
    AF372979 Genomic DNA. Translation: AAM20894.1 .
    BC025643 mRNA. Translation: AAH25643.1 .
    CCDSi CCDS39972.1.
    RefSeqi NP_033858.2. NM_009728.2.
    XP_006540643.1. XM_006540580.1.
    XP_006540644.1. XM_006540581.1.
    UniGenei Mm.135129.

    3D structure databases

    ProteinModelPortali O54827.
    SMRi O54827. Positions 757-1066.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O54827.

    Proteomic databases

    PRIDEi O54827.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000168747 ; ENSMUSP00000129811 ; ENSMUSG00000025324 .
    GeneIDi 11982.
    KEGGi mmu:11982.
    UCSCi uc009hef.2. mouse.

    Organism-specific databases

    CTDi 57194.
    MGIi MGI:1330809. Atp10a.

    Phylogenomic databases

    eggNOGi COG0474.
    HOVERGENi HBG107129.
    InParanoidi O54827.
    KOi K01530.
    OMAi WRYQEKK.
    OrthoDBi EOG76471S.
    PhylomeDBi O54827.
    TreeFami TF354252.

    Miscellaneous databases

    NextBioi 280129.
    PROi O54827.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54827.
    Bgeei O54827.
    CleanExi MM_ATP10A.
    Genevestigatori O54827.

    Family and domain databases

    Gene3Di 2.70.150.10. 2 hits.
    3.40.1110.10. 3 hits.
    3.40.50.1000. 2 hits.
    InterProi IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    PANTHERi PTHR24092. PTHR24092. 1 hit.
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01652. ATPase-Plipid. 2 hits.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of putative transbilayer amphipath transporters."
      Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A.
      Physiol. Genomics 1:139-150(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Teratocarcinoma.
    2. "Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs."
      Halleck M.S., Pradhan D., Blackman C.F., Berkes C., Williamson P.L., Schlegel R.A.
      Genome Res. 8:354-361(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-435.
    3. "An aminophospholipid translocase associated with body fat and type 2 diabetes phenotypes."
      Dhar M., Hauser L., Johnson D.
      Obes. Res. 10:695-702(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-1508.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 840-1508.
    5. "A novel ATPase on mouse chromosome 7 is a candidate gene for increased body fat."
      Dhar M., Webb L.S., Smith L., Hauser L., Johnson D., West D.B.
      Physiol. Genomics 4:93-100(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAT10A_MOUSE
    AccessioniPrimary (citable) accession number: O54827
    Secondary accession number(s): Q8R3B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 130 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3