O54825 (BYST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bystin

Gene names

Name:

Bysl

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. Ref.4 Ref.5
Subunit structure	Binds trophinin, tastin and cytokeratins By similarity.
Subcellular location	Cytoplasm. Nucleus › nucleolus. Note: Associated with 40S ribosomal subunits. Ref.5
Tissue specificity	High levels in preimplantation embryos, bone marrow, brain, testis and ovary. Ref.5
Developmental stage	High levels of maternal transcript in unfertilized eggs. High levels up to blastocyst stage. Ref.5
Disruption phenotype	Mice show embryonic lethality around stage E6.5 shortly after implantation. Mice lacking maternal Bysl transcript upon injection of siRNA into fertilized eggs are arrested at the 16-cell stage, fail to induce trophectoderm, and show altered morphology of developing nucleoli. Embryonic stem cells lacking Bysl show accumulation of pre-20S rRNA precursors and a reduction of 40S ribosomal subunits in the cytoplasm. Ref.4
Sequence similarities	Belongs to the bystin family.
Sequence caution	The sequence AAH17530.3 differs from that shown. Reason: Erroneous initiation. The sequence BAB31619.2 differs from that shown. Reason: Erroneous initiation. The sequence BAC38736.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from sequence or structural similarity PubMed 11252172. Source: MGI cell proliferation Inferred from mutant phenotype Ref.5. Source: MGI maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.5. Source: MGI trophectodermal cell differentiation Inferred from mutant phenotype Ref.5. Source: MGI
Cellular_component	apical part of cell Inferred from direct assay Ref.4. Source: MGI cytoplasm Inferred from direct assay Ref.4. Source: MGI nucleolus Inferred from direct assay Ref.4Ref.5. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Bystin		PRO_0000186115
Amino acid modifications
	Modified residue	97	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	166	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	284	1	A → V in BAE40242. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54825 [UniParc]. Last modified May 30, 2006. Version 3. Checksum: EFA148BED2072B72	FASTA	436	49,784
	10 20 30 40 50 60 MPKFKVTRGA SNREKHAPLA EQILAGNAVR AGTREKRRGR EVEEEEEYVG PRLSRRILQQ 70 80 90 100 110 120 ARQQQEELET DHGAGDRSAP PRERATRLGP GLPQDGSDEE DEEWPTLEKA AKMAGVDHQA 130 140 150 160 170 180 EVIVDPEDER AIEMFMNKNP PVRRTLADII MEKLTEKQTE VETVMSEVSG FPMPQLDPRV 190 200 210 220 230 240 LEVYRGVREV LCKYRSGKLP KAFKVIPALS NWEQILYVTE PEAWTAAAMY QATRIFASNL 250 260 270 280 290 300 KERMAQRFYN LVLLPRVRDD IAEYKRLNFH LYMALKKALF KPGAWFKGIL IPLCESGTCT 310 320 330 340 350 360 LREAIIVGSI ITKCSIPVLH SSAAMLKIAE MEYSGANSIF LRLLLDKKYA LPYRVLDALV 370 380 390 400 410 420 FHFLAFRTEK RQLPVLWHQC LLTLAQRYKA DLATEQKEAL LELLRLQPHP QLSPEIRREL 430 QSAVPRDVED GGVTME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: BALB/c and C57BL/6J. Tissue: Embryo, Embryonic spinal cord and Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Salivary gland.
[3]	"Characterization of mouse bystin." Suzuki N., Fukuda M.N. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 151-436.
[4]	"The Bysl gene product, bystin, is essential for survival of mouse embryos." Aoki R., Suzuki N., Paria B.C., Sugihara K., Akama T.O., Raab G., Miyoshi M., Nadano D., Fukuda M.N. FEBS Lett. 580:6062-6068(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]	"Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis." Adachi K., Soeta-Saneyoshi C., Sagara H., Iwakura Y. Mol. Cell. Biol. 27:2202-2214(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Liver.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Macrophage.
[8]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK019239 mRNA. Translation: BAB31619.2. Different initiation. AK083033 mRNA. Translation: BAC38736.1. Different initiation. AK143027 mRNA. Translation: BAE25255.1. AK168301 mRNA. Translation: BAE40242.1. BC017530 mRNA. Translation: AAH17530.3. Different initiation. AF007802 mRNA. Translation: AAB94491.1.
IPI	IPI00118762.
RefSeq	NP_058555.3. NM_016859.3.
UniGene	Mm.472101.
3D structure databases
ProteinModelPortal	O54825.
ModBase	Search...
Protein-protein interaction databases
IntAct	O54825. 1 interaction.
PTM databases
PhosphoSite	O54825.
Proteomic databases
PaxDb	O54825.
PRIDE	O54825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024783; ENSMUSP00000024783; ENSMUSG00000023988.
GeneID	53414.
KEGG	mmu:53414.
UCSC	uc008cvq.2. mouse.
Organism-specific databases
CTD	705.
MGI	MGI:1858419. Bysl.
Phylogenomic databases
eggNOG	NOG280148.
GeneTree	ENSGT00390000007241.
HOGENOM	HOG000178028.
HOVERGEN	HBG024425.
InParanoid	O54825.
KO	K14797.
OMA	LNFHLYQ.
OrthoDB	EOG4VHK6H.
Gene expression databases
ArrayExpress	O54825.
Bgee	O54825.
CleanEx	MM_BYSL.
Genevestigator	O54825.
GermOnline	ENSMUSG00000023988. Mus musculus.
Family and domain databases
InterPro	IPR007955. Bystin. [Graphical view]
PANTHER	PTHR12821. PTHR12821. 1 hit.
Pfam	PF05291. Bystin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BYSL. mouse.
NextBio	310229.
SOURCE	Search...

Entry information

Entry name

BYST_MOUSE

Accession

Primary (citable) accession number: O54825
Secondary accession number(s): Q3THF4, Q3UPY9, Q8VD68

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 30, 2006
Last modified:	April 3, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program