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Protein

Pro-interleukin-16

Gene

Il16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

GO - Molecular functioni

  • cytokine activity Source: MGI

GO - Biological processi

  • induction of positive chemotaxis Source: MGI
  • leukocyte chemotaxis Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-449147. Signaling by Interleukins.

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-interleukin-16
Cleaved into the following chain:
Interleukin-16
Short name:
IL-16
Alternative name(s):
Lymphocyte chemoattractant factor
Short name:
LCF
Gene namesi
Name:Il16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1270855. Il16.

Subcellular locationi

Isoform 1 :
  • Cytoplasm 1 Publication

  • Note: Colocalizes with GRIN2C in neuronal cell bodies and neurites.
Isoform 2 :

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular space Source: UniProtKB-KW
  • intracellular Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003775461 – 1322Pro-interleukin-16Add BLAST1322
ChainiPRO_00000154181205 – 1322Interleukin-16By similarityAdd BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei917PhosphoserineBy similarity1

Post-translational modificationi

Synthesized as a chemo-attractant inactive precursor which is proteolytically cleaved by caspase-3 to yield IL-16.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54824.
PaxDbiO54824.
PeptideAtlasiO54824.
PRIDEiO54824.

PTM databases

iPTMnetiO54824.
PhosphoSitePlusiO54824.

Expressioni

Tissue specificityi

Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Gene expression databases

BgeeiENSMUSG00000001741.
CleanExiMM_IL16.
ExpressionAtlasiO54824. baseline and differential.
GenevisibleiO54824. MM.

Interactioni

Subunit structurei

Homotetramer (Probable). Isoform 2 interacts with GRIN2A. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C. Isoform 2 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GABPB1. Isoform 2 interacts (via PDZ 3 domain) with HDAC3 (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
ItpkcQ7TS723EBI-641708,EBI-648015

GO - Molecular functioni

  • cytokine activity Source: MGI

Protein-protein interaction databases

BioGridi200618. 13 interactors.
IntActiO54824. 14 interactors.
MINTiMINT-103508.
STRINGi10090.ENSMUSP00000001792.

Structurei

3D structure databases

ProteinModelPortaliO54824.
SMRiO54824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini213 – 299PDZ 1PROSITE-ProRule annotationAdd BLAST87
Domaini352 – 437PDZ 2PROSITE-ProRule annotationAdd BLAST86
Domaini1105 – 1190PDZ 3PROSITE-ProRule annotationAdd BLAST86
Domaini1226 – 1309PDZ 4PROSITE-ProRule annotationAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 449Interaction with GRIN2ABy similarityAdd BLAST267
Regioni1099 – 1195Interaction with PPP1R12A, PPP1R12B and PPP1R12CBy similarityAdd BLAST97

Sequence similaritiesi

Contains 4 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00760000119017.
HOGENOMiHOG000081749.
HOVERGENiHBG000100.
InParanoidiO54824.
OMAiAVIVTRK.
OrthoDBiEOG091G0G6P.
TreeFamiTF326303.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR020450. IL-16.
IPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 2 hits.
[Graphical view]
PRINTSiPR01931. INTRLEUKIN16.
SMARTiSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS50106. PDZ. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: O54824-1) [UniParc]FASTAAdd to basket
Also known as: NIL-16

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPHGHSGKS RKSTKFRSIS RSLILCNAKT SDDGSSPDEK YPDPFETSLC
60 70 80 90 100
QGKEGFFHSS MQLADTFEAG LSNIPDLALA SDSAQLAAAG SDRGKHCRKM
110 120 130 140 150
FFMKESSSTS SKEKSGKPEA QSSSFLFPKA CHQRTRSNST SVNPYSAGEI
160 170 180 190 200
DFPMTKKSAA PTDRQPYSLC SNRKSLSQQL DYPILGTARP TRSLSTAQLG
210 220 230 240 250
QLSGGLQASV ISNIVLMKGQ AKGLGFSIVG GKDSIYGPIG IYVKSIFAGG
260 270 280 290 300
AAAADGRLQE GDEILELNGE SMAGLTHQDA LQKFKQAKKG LLTLTVRTRL
310 320 330 340 350
TTPPSLCSHL SPPLCRSLSS STCGAQDSSP FSLESPASPA STAKPNYRIM
360 370 380 390 400
VEVSLKKEAG VGLGIGLCSI PYFQCISGIF VHTLSPGSVA HLDGRLRCGD
410 420 430 440 450
EIVEINDSPV HCLTLNEVYT ILSHCDPGPV PIIVSRHPDP QVSEQQLKEA
460 470 480 490 500
VAQAVEGVKF GKDRHQWSLE GVKRLESSWH GRPTLEKERE KHSAPPHRRA
510 520 530 540 550
QKIMVRSSSD SSYMSGSPGG SPCSAGAEPQ PSEREGSTHS PSLSPGEEQE
560 570 580 590 600
PCPGVPSRPQ QESPPLPESL ERESHPPLRL KKSFEILVRK PTSSKPKPPP
610 620 630 640 650
RKYFKNDSEP QKKLEEKEKV TDPSGHTLPT CSQETRELLP LLLQEDTAGR
660 670 680 690 700
APCTAACCPG PAASTQTSSS TEGESRRSAS PETPASPGKH PLLKRQARMD
710 720 730 740 750
YSFDITAEDP WVRISDCIKN LFSPIMSENH SHTPLQPNTS LGEEDGTQGC
760 770 780 790 800
PEGGLSKMDA ANGAPRVYKS ADGSTVKKGP PVAPKPAWFR QSLKGLRNRA
810 820 830 840 850
PDPRRPPEVA SAIQPTPVSR DPPGPQPQAS SSIRQRISSF ENFGSSQLPD
860 870 880 890 900
RGVQRLSLQP SSGETTKFPG KQDGGRFSGL LGQGATVTAK HRQTEVESMS
910 920 930 940 950
TTFPNSSEVR DPGLPESPPP GQRPSTKALS PDPLLRLLTT QSEDTQGPGL
960 970 980 990 1000
KMPSQRARSF PLTRTQSCET KLLDEKASKL YSISSQLSSA VMKSLLCLPS
1010 1020 1030 1040 1050
SVSCGQITCI PKERVSPKSP CNNSSAAEGF GEAMASDTGF SLNLSELREY
1060 1070 1080 1090 1100
SEGLTEPGET EDRNHCSSQA GQSVISLLSA EELEKLIEEV RVLDEATLKQ
1110 1120 1130 1140 1150
LDSIHVTILH KEEGAGLGFS LAGGADLENK VITVHRVFPN GLASQEGTIQ
1160 1170 1180 1190 1200
KGNEVLSING KSLKGATHND ALAILRQARD PRQAVIVTRR TTVEATHDLN
1210 1220 1230 1240 1250
SSTDSAASAS AASDISVESK EATVCTVTLE KTSAGLGFSL EGGKGSLHGD
1260 1270 1280 1290 1300
KPLTINRIFK GTEQGEMVQP GDEILQLAGT AVQGLTRFEA WNVIKALPDG
1310 1320
PVTIVIRRTS LQCKQTTASA DS
Note: Produced by alternative promoter usage. Is probably proteolytically processed to yield IL-16.
Length:1,322
Mass (Da):141,435
Last modified:June 16, 2009 - v3
Checksum:iDDB94003A5DCB738
GO
Isoform 2 (identifier: O54824-2) [UniParc]FASTAAdd to basket
Also known as: Pro-IL-16

The sequence of this isoform differs from the canonical sequence as follows:
     1-698: Missing.

Note: Produced by alternative promoter usage. Is proteolytically processed to yield IL-16.
Show »
Length:624
Mass (Da):66,471
Checksum:iE46375FAABE9D6E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti264I → S in BAD90484 (Ref. 5) Curated1
Sequence conflicti733T → R in AAC04383 (PubMed:9637508).Curated1
Sequence conflicti921G → S in AAC16039 (PubMed:9510557).Curated1
Sequence conflicti1067S → P in AAC16039 (PubMed:9510557).Curated1
Sequence conflicti1224V → A in AAC16039 (PubMed:9510557).Curated1
Sequence conflicti1262 – 1264TEQ → DRT in AAC04383 (PubMed:9637508).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374601 – 698Missing in isoform 2. 4 PublicationsAdd BLAST698

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006001 mRNA. Translation: AAC04383.1.
AF017111 mRNA. Translation: AAC16039.1.
AF175292 mRNA. Translation: AAD55393.1.
AK038319 mRNA. Translation: BAC29967.1.
AK169551 mRNA. Translation: BAE41224.1.
AK220455 mRNA. Translation: BAD90484.1.
CH466543 Genomic DNA. Translation: EDL06877.1.
BC026894 mRNA. Translation: AAH26894.1.
BC058709 mRNA. Translation: AAH58709.1.
CCDSiCCDS21412.1. [O54824-1]
RefSeqiNP_034681.2. NM_010551.3. [O54824-1]
XP_006507449.1. XM_006507386.3. [O54824-1]
XP_011239983.1. XM_011241681.2. [O54824-1]
UniGeneiMm.10137.

Genome annotation databases

EnsembliENSMUST00000001792; ENSMUSP00000001792; ENSMUSG00000001741. [O54824-1]
ENSMUST00000145610; ENSMUSP00000146496; ENSMUSG00000001741. [O54824-2]
GeneIDi16170.
KEGGimmu:16170.
UCSCiuc009idr.1. mouse. [O54824-2]
uc009ids.1. mouse. [O54824-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006001 mRNA. Translation: AAC04383.1.
AF017111 mRNA. Translation: AAC16039.1.
AF175292 mRNA. Translation: AAD55393.1.
AK038319 mRNA. Translation: BAC29967.1.
AK169551 mRNA. Translation: BAE41224.1.
AK220455 mRNA. Translation: BAD90484.1.
CH466543 Genomic DNA. Translation: EDL06877.1.
BC026894 mRNA. Translation: AAH26894.1.
BC058709 mRNA. Translation: AAH58709.1.
CCDSiCCDS21412.1. [O54824-1]
RefSeqiNP_034681.2. NM_010551.3. [O54824-1]
XP_006507449.1. XM_006507386.3. [O54824-1]
XP_011239983.1. XM_011241681.2. [O54824-1]
UniGeneiMm.10137.

3D structure databases

ProteinModelPortaliO54824.
SMRiO54824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200618. 13 interactors.
IntActiO54824. 14 interactors.
MINTiMINT-103508.
STRINGi10090.ENSMUSP00000001792.

PTM databases

iPTMnetiO54824.
PhosphoSitePlusiO54824.

Proteomic databases

MaxQBiO54824.
PaxDbiO54824.
PeptideAtlasiO54824.
PRIDEiO54824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001792; ENSMUSP00000001792; ENSMUSG00000001741. [O54824-1]
ENSMUST00000145610; ENSMUSP00000146496; ENSMUSG00000001741. [O54824-2]
GeneIDi16170.
KEGGimmu:16170.
UCSCiuc009idr.1. mouse. [O54824-2]
uc009ids.1. mouse. [O54824-1]

Organism-specific databases

CTDi3603.
MGIiMGI:1270855. Il16.

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00760000119017.
HOGENOMiHOG000081749.
HOVERGENiHBG000100.
InParanoidiO54824.
OMAiAVIVTRK.
OrthoDBiEOG091G0G6P.
TreeFamiTF326303.

Enzyme and pathway databases

ReactomeiR-MMU-449147. Signaling by Interleukins.

Miscellaneous databases

PROiO54824.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001741.
CleanExiMM_IL16.
ExpressionAtlasiO54824. baseline and differential.
GenevisibleiO54824. MM.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR020450. IL-16.
IPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 2 hits.
[Graphical view]
PRINTSiPR01931. INTRLEUKIN16.
SMARTiSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS50106. PDZ. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIL16_MOUSE
AccessioniPrimary (citable) accession number: O54824
Secondary accession number(s): O70236
, Q3TEM4, Q5DTR5, Q8R0G5, Q9QZP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.