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O54824 (IL16_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pro-interleukin-16

Cleaved into the following chain:

  1. Interleukin-16
    Short name=IL-16
    Alternative name(s):
    Lymphocyte chemoattractant factor
    Short name=LCF
Gene names
Name:Il16
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1322 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Subunit structure

Homotetramer Probable. Isoform 2 interacts with GRIN2A. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C. Isoform 2 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3interacts with GABPB1. Isoform 2 interacts (via PDZ 3 domain) with HDAC3 By similarity. Ref.3

Subcellular location

Secreted By similarity Ref.3.

Isoform 1: Cytoplasm. Note: Colocalizes with GRIN2C in neuronal cell bodies and neurites. Ref.3

Isoform 2: Cytoplasm. Nucleus By similarity Ref.3.

Tissue specificity

Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung. Ref.1 Ref.3

Post-translational modification

Synthesized as a chemo-attractant inactive precursor which is proteolytically cleaved by caspase-3 to yield IL-16. Ref.3

Sequence similarities

Contains 4 PDZ (DHR) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ItpkcQ7TS723EBI-641708,EBI-648015

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform 1 (identifier: O54824-1)

Also known as: NIL-16;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage. Is probably proteolytically processed to yield IL-16.
Isoform 2 (identifier: O54824-2)

Also known as: Pro-IL-16;

The sequence of this isoform differs from the canonical sequence as follows:
     1-698: Missing.
Note: Produced by alternative promoter usage. Is proteolytically processed to yield IL-16.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13221322Pro-interleukin-16
PRO_0000377546
Chain1205 – 1322118Interleukin-16 By similarity
PRO_0000015418

Regions

Domain213 – 29987PDZ 1
Domain352 – 43786PDZ 2
Domain1105 – 119086PDZ 3
Domain1226 – 130984PDZ 4
Region183 – 449267Interaction with GRIN2A By similarity
Region1099 – 119597Interaction with PPP1R12A, PPP1R12B and PPP1R12C By similarity

Natural variations

Alternative sequence1 – 698698Missing in isoform 2.
VSP_037460

Experimental info

Sequence conflict2641I → S in BAD90484. Ref.5
Sequence conflict7331T → R in AAC04383. Ref.1
Sequence conflict9211G → S in AAC16039. Ref.2
Sequence conflict10671S → P in AAC16039. Ref.2
Sequence conflict12241V → A in AAC16039. Ref.2
Sequence conflict1262 – 12643TEQ → DRT in AAC04383. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NIL-16) [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: DDB94003A5DCB738

FASTA1,322141,435
        10         20         30         40         50         60 
MEPHGHSGKS RKSTKFRSIS RSLILCNAKT SDDGSSPDEK YPDPFETSLC QGKEGFFHSS 

        70         80         90        100        110        120 
MQLADTFEAG LSNIPDLALA SDSAQLAAAG SDRGKHCRKM FFMKESSSTS SKEKSGKPEA 

       130        140        150        160        170        180 
QSSSFLFPKA CHQRTRSNST SVNPYSAGEI DFPMTKKSAA PTDRQPYSLC SNRKSLSQQL 

       190        200        210        220        230        240 
DYPILGTARP TRSLSTAQLG QLSGGLQASV ISNIVLMKGQ AKGLGFSIVG GKDSIYGPIG 

       250        260        270        280        290        300 
IYVKSIFAGG AAAADGRLQE GDEILELNGE SMAGLTHQDA LQKFKQAKKG LLTLTVRTRL 

       310        320        330        340        350        360 
TTPPSLCSHL SPPLCRSLSS STCGAQDSSP FSLESPASPA STAKPNYRIM VEVSLKKEAG 

       370        380        390        400        410        420 
VGLGIGLCSI PYFQCISGIF VHTLSPGSVA HLDGRLRCGD EIVEINDSPV HCLTLNEVYT 

       430        440        450        460        470        480 
ILSHCDPGPV PIIVSRHPDP QVSEQQLKEA VAQAVEGVKF GKDRHQWSLE GVKRLESSWH 

       490        500        510        520        530        540 
GRPTLEKERE KHSAPPHRRA QKIMVRSSSD SSYMSGSPGG SPCSAGAEPQ PSEREGSTHS 

       550        560        570        580        590        600 
PSLSPGEEQE PCPGVPSRPQ QESPPLPESL ERESHPPLRL KKSFEILVRK PTSSKPKPPP 

       610        620        630        640        650        660 
RKYFKNDSEP QKKLEEKEKV TDPSGHTLPT CSQETRELLP LLLQEDTAGR APCTAACCPG 

       670        680        690        700        710        720 
PAASTQTSSS TEGESRRSAS PETPASPGKH PLLKRQARMD YSFDITAEDP WVRISDCIKN 

       730        740        750        760        770        780 
LFSPIMSENH SHTPLQPNTS LGEEDGTQGC PEGGLSKMDA ANGAPRVYKS ADGSTVKKGP 

       790        800        810        820        830        840 
PVAPKPAWFR QSLKGLRNRA PDPRRPPEVA SAIQPTPVSR DPPGPQPQAS SSIRQRISSF 

       850        860        870        880        890        900 
ENFGSSQLPD RGVQRLSLQP SSGETTKFPG KQDGGRFSGL LGQGATVTAK HRQTEVESMS 

       910        920        930        940        950        960 
TTFPNSSEVR DPGLPESPPP GQRPSTKALS PDPLLRLLTT QSEDTQGPGL KMPSQRARSF 

       970        980        990       1000       1010       1020 
PLTRTQSCET KLLDEKASKL YSISSQLSSA VMKSLLCLPS SVSCGQITCI PKERVSPKSP 

      1030       1040       1050       1060       1070       1080 
CNNSSAAEGF GEAMASDTGF SLNLSELREY SEGLTEPGET EDRNHCSSQA GQSVISLLSA 

      1090       1100       1110       1120       1130       1140 
EELEKLIEEV RVLDEATLKQ LDSIHVTILH KEEGAGLGFS LAGGADLENK VITVHRVFPN 

      1150       1160       1170       1180       1190       1200 
GLASQEGTIQ KGNEVLSING KSLKGATHND ALAILRQARD PRQAVIVTRR TTVEATHDLN 

      1210       1220       1230       1240       1250       1260 
SSTDSAASAS AASDISVESK EATVCTVTLE KTSAGLGFSL EGGKGSLHGD KPLTINRIFK 

      1270       1280       1290       1300       1310       1320 
GTEQGEMVQP GDEILQLAGT AVQGLTRFEA WNVIKALPDG PVTIVIRRTS LQCKQTTASA 


DS 

« Hide

Isoform 2 (Pro-IL-16) [UniParc].

Checksum: E46375FAABE9D6E0
Show »

FASTA62466,471

References

« Hide 'large scale' references
[1]"Conservation of structure and function between human and murine IL-16."
Keane J., Nicoll J., Kim S., Wu D.M.H., Cruikshank W.W., Brazer W., Natke B., Zhang Y., Center D.M., Kornfeld H.
J. Immunol. 160:5945-5954(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY (ISOFORM 2).
Strain: NFS.
Tissue: Spleen.
[2]"Molecular cloning and sequence analysis of interleukin 16 from nonhuman primates and from the mouse."
Bannert N., Adler H.S., Werner A., Baier M., Kurth R.
Immunogenetics 47:390-397(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein."
Kurschner C., Yuzaki M.
J. Neurosci. 19:7770-7780(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION (ISOFORM 1), TISSUE SPECIFICITY (ISOFORM 1), INTERACTION WITH GRIN2A; GRIN2D; KCNJ10; KCNJ15 AND CACNA1C, PROTEOLYTIC PROCESSING.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Thymus.
[5]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Fetal brain.
[6]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF006001 mRNA. Translation: AAC04383.1.
AF017111 mRNA. Translation: AAC16039.1.
AF175292 mRNA. Translation: AAD55393.1.
AK038319 mRNA. Translation: BAC29967.1.
AK169551 mRNA. Translation: BAE41224.1.
AK220455 mRNA. Translation: BAD90484.1.
CH466543 Genomic DNA. Translation: EDL06877.1.
BC026894 mRNA. Translation: AAH26894.1.
BC058709 mRNA. Translation: AAH58709.1.
RefSeqNP_034681.2. NM_010551.3.
XP_006507449.1. XM_006507386.1.
UniGeneMm.10137.

3D structure databases

ProteinModelPortalO54824.
SMRO54824. Positions 213-441, 1096-1322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO54824. 14 interactions.
MINTMINT-103508.

PTM databases

PhosphoSiteO54824.

Proteomic databases

PaxDbO54824.
PRIDEO54824.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001792; ENSMUSP00000001792; ENSMUSG00000001741. [O54824-1]
GeneID16170.
KEGGmmu:16170.
UCSCuc009idr.1. mouse. [O54824-2]
uc009ids.1. mouse. [O54824-1]

Organism-specific databases

CTD3603.
MGIMGI:1270855. Il16.

Phylogenomic databases

eggNOGNOG150595.
GeneTreeENSGT00740000115038.
HOGENOMHOG000081749.
HOVERGENHBG000100.
InParanoidQ5DTR5.
OMAILSHCDP.
OrthoDBEOG79W94J.
TreeFamTF326303.

Gene expression databases

ArrayExpressO54824.
BgeeO54824.
CleanExMM_IL16.
GenevestigatorO54824.

Family and domain databases

Gene3D2.30.42.10. 4 hits.
InterProIPR020450. IL-16.
IPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 4 hits.
[Graphical view]
PRINTSPR01931. INTRLEUKIN16.
SMARTSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMSSF50156. SSF50156. 4 hits.
PROSITEPS50106. PDZ. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio289013.
PROO54824.
SOURCESearch...

Entry information

Entry nameIL16_MOUSE
AccessionPrimary (citable) accession number: O54824
Secondary accession number(s): O70236 expand/collapse secondary AC list , Q3TEM4, Q5DTR5, Q8R0G5, Q9QZP6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot