Reviewed, UniProtKB/Swiss-Prot O54819 (TFPI1_MOUSE)

Names and origin

Protein names

Recommended name:
    Tissue factor pathway inhibitor
      Short name=TFPI
Alternative name(s):
    Lipoprotein-associated coagulation inhibitor
      Short name=LACI
    Extrinsic pathway inhibitor
      Short name=EPI

Gene names

Name:

Tfpi

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma By similarity.
Subcellular location	Secreted.
Tissue specificity	Isoform alpha is expressed in heart and spleen; isoform beta in heart and lung.
Domain	This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa By similarity.
Sequence similarities	Contains 3 BPTI/Kunitz inhibitor domains.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha (identifier: O54819-1)

Also known as: TFPIalpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: O54819-2)

Also known as: TFPIbeta;

The sequence of this isoform differs from the canonical sequence as follows:
     218-253: DYRGRPWCLQPADSGLCKASERRFYYNSATGKCHRF → VTKEETNGGWKNADYTYQGFLSSVYIHVLYFVFRIG
     254-306: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 306	278	Tissue factor pathway inhibitor		PRO_0000016873
Regions
	Domain	50 – 100	51	BPTI/Kunitz inhibitor 1
	Domain	121 – 171	51	BPTI/Kunitz inhibitor 2
	Domain	225 – 275	51	BPTI/Kunitz inhibitor 3
Sites
	Site	60 – 61	2	Reactive bond By similarity
	Site	131 – 132	2	Reactive bond By similarity
	Site	235 – 236	2	Reactive bond By similarity
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 100		By similarity
	Disulfide bond	59 ↔ 83		By similarity
	Disulfide bond	75 ↔ 96		By similarity
	Disulfide bond	121 ↔ 171		By similarity
	Disulfide bond	130 ↔ 154		By similarity
	Disulfide bond	146 ↔ 167		By similarity
	Disulfide bond	225 ↔ 275		By similarity
	Disulfide bond	234 ↔ 258		By similarity
	Disulfide bond	250 ↔ 271		By similarity
Natural variations
	Alternative sequence	218 – 253	36	DYRGR…KCHRF → VTKEETNGGWKNADYTYQGF LSSVYIHVLYFVFRIG in isoform Beta.		VSP_003032
	Alternative sequence	254 – 306	53	Missing in isoform Beta.		VSP_003033
Experimental info
	Sequence conflict	68	1	F → L in AAD01586. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (TFPIalpha) [UniParc]. Last modified June 1, 1998. Version 1. Checksum: D3EA3297E4B6A359	FASTA	306	34,987
	10 20 30 40 50 60 MTYKMKKEYA FWATVCLLLS LVPEFLNALS EEADDTDSEL GSMKPLHTFC AMKADDGPCK 70 80 90 100 110 120 AMIRSYFFNM YTHQCEEFIY GGCEGNENRF DTLEECKKTC IPGYEKTAVK AASGAERPDF 130 140 150 160 170 180 CFLEEDPGLC RGYMKRYLYN NQTKQCERFV YGGCLGNRNN FETLDECKKI CENPVHSPSP 190 200 210 220 230 240 VNEVQMSDYV TDGNTVTDRS TVNNIVVPQS PKVPRRRDYR GRPWCLQPAD SGLCKASERR 250 260 270 280 290 300 FYYNSATGKC HRFNYTGCGG NNNNFTTRRR CLRSCKTGLI KNKSKGVVKI QRRKAPFVKV VYESIN
	Isoform Beta (TFPIbeta). Checksum: FDEAB02B634A810B	FASTA	253	28,949
	10 20 30 40 50 60 MTYKMKKEYA FWATVCLLLS LVPEFLNALS EEADDTDSEL GSMKPLHTFC AMKADDGPCK 70 80 90 100 110 120 AMIRSYFFNM YTHQCEEFIY GGCEGNENRF DTLEECKKTC IPGYEKTAVK AASGAERPDF 130 140 150 160 170 180 CFLEEDPGLC RGYMKRYLYN NQTKQCERFV YGGCLGNRNN FETLDECKKI CENPVHSPSP 190 200 210 220 230 240 VNEVQMSDYV TDGNTVTDRS TVNNIVVPQS PKVPRRRVTK EETNGGWKNA DYTYQGFLSS 250 VYIHVLYFVF RIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and characterization of mouse tissue factor pathway inhibitor (TFPI)." Chang J.-Y., Monroe D.M., Oliver J.A., Liles D.K., Roberts H.R. Thromb. Haemost. 79:306-309(1998) [PubMed: 9493581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA). Strain: 129.
[2]	"TFPIbeta, a second product from the mouse tissue factor pathway inhibitor (TFPI) gene." Chang J.-Y., Monroe D.M., Oliver J.A., Roberts H.R. Thromb. Haemost. 81:45-49(1999) [PubMed: 9974373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Strain: C57BL/6J. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF004833 mRNA. Translation: AAC40035.1. AF016313 mRNA. Translation: AAD01586.1. AK028356 mRNA. Translation: BAC25901.1.
IPI	IPI00118737. IPI00229887.
RefSeq	NP_035706.1.
UniGene	Mm.124316 Mm.447013
3D structure databases
HSSP	HSSP built from PDB template 1TFX based on UniProtKB P10646.
SMR	O54819. Positions 117-173.
ModBase	Search...
PTM databases
PhosphoSite	O54819.
Genome annotation databases
Ensembl	ENSMUSG00000027082. Mus musculus. [Contig view]
GeneID	21788.
KEGG	mmu:21788.
Organism-specific databases
MGI	MGI:1095418. Tfpi.
Phylogenomic databases
HOGENOM	O54819.
HOVERGEN	O54819.
OMA	O54819. FIQRISK.
Gene expression databases
ArrayExpress	O54819.
Bgee	O54819.
CleanEx	MM_TFPI.
GermOnline	ENSMUSG00000027082. Mus musculus.
Family and domain databases
InterPro	IPR002223. Prot_inh_Kunz-m. IPR008296. Prot_inhib_I2_TFPI. [Graphical view]
Gene3D	G3DSA:4.10.410.10. Prot_inh_Kunz-m. 2 hits.
Pfam	PF00014. Kunitz_BPTI. 3 hits. [Graphical view]
PIRSF	PIRSF001620. TFPI. 1 hit.
ProDom	PD000222. Prot_Inh_Kunz-m. 3 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00131. KU. 3 hits. [Graphical view]
PROSITE	PS00280. BPTI_KUNITZ_1. 3 hits. PS50279. BPTI_KUNITZ_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301144.
SOURCE	Search...

Entry information

Entry name

TFPI1_MOUSE

Accession

Primary (citable) accession number: O54819
Secondary accession number(s): Q9Z2U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)