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O54804 (CHKA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline kinase alpha

Short name=CK
EC=2.7.1.32
Alternative name(s):
CHETK-alpha
Ethanolamine kinase
Short name=EK
EC=2.7.1.82
Gene names
Name:Chka
Synonyms:Chk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline By similarity.

Catalytic activity

ATP + choline = ADP + phosphocholine.

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Subunit structure

Homodimer, and heterodimer with CHKB. Ref.7

Subcellular location

Cytoplasm.

Tissue specificity

Expressed ubiquitously with the highest level in testis.

Disruption phenotype

Death at an early embryonic stage. Embryos die after 3 to 5 days of development. Ref.8

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Sequence caution

The sequence AAH56758.1 differs from that shown. Reason: Intron retention.

The sequence BAA88154.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAB29191.1 differs from that shown. Reason: Intron retention.

The sequence BAC34841.1 differs from that shown. Reason: Intron retention.

The sequence BAC35539.1 differs from that shown. Reason: Intron retention.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O54804-1)

Also known as: CHETK-alpha2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O54804-2)

Also known as: CHETK-alpha1;

The sequence of this isoform differs from the canonical sequence as follows:
     151-168: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Choline kinase alpha
PRO_0000206220

Regions

Nucleotide binding113 – 1197ATP By similarity
Nucleotide binding203 – 2097ATP By similarity
Region115 – 1173Substrate binding By similarity
Compositional bias53 – 8129Pro-rich

Sites

Binding site1421ATP By similarity
Binding site3041ATP By similarity
Binding site3261ATP By similarity

Natural variations

Alternative sequence151 – 16818Missing in isoform 2.
VSP_009684

Experimental info

Sequence conflict1931F → Y in BAA24898. Ref.1
Sequence conflict1931F → Y in BAA88154. Ref.2
Sequence conflict1931F → Y in BAA88153. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CHETK-alpha2) [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: 66042FEE92C333D4

FASTA45351,985
        10         20         30         40         50         60 
MKTKFCTGGE AEPSPLGLLL SCGGNAAPTP GVGQQRDAAG ELESKQLGGR TQPLALPPPP 

        70         80         90        100        110        120 
PPPLPLPPPP SPPLADEQPE PRTRRRAYLW CKEFLPGAWR GLREDQFHIS VIRGGLSNML 

       130        140        150        160        170        180 
FQCSLPDSIA SVGDEPRKVL LRLYGAILKM RSCNKEGSEQ AQNENEFQGA EAMVLESVMF 

       190        200        210        220        230        240 
AILAERSLGP KLFGIFPQGR LEQFIPSRRL DTEELRLPDI SAEIAEKMAT FHGMKMPFNK 

       250        260        270        280        290        300 
EPKWLFGTME KYLNQVLRLK FSREARVQQL HKILSYNLPL ELENLRSLLQ YTRSPVVFCH 

       310        320        330        340        350        360 
NDCQEGNILL LEGQENSERR KLMLIDFEYS SYNYRGFDIG NHFCEWMYDY TYEKYPFFRA 

       370        380        390        400        410        420 
NIQKYPSRKQ QLHFISSYLT TFQNDFESLS SEEQFATKED MLLEVNRFAL ASHFLWGLWS 

       430        440        450 
IVQAKISSIE FGYMEYAQAR FEAYFDQKRK LGV 

« Hide

Isoform 2 (CHETK-alpha1) [UniParc].

Checksum: DC7377434296F68A
Show »

FASTA43549,904

References

« Hide 'large scale' references
[1]"Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs."
Aoyama C., Nakashima K., Ishidate K.
Biochim. Biophys. Acta 1393:179-185(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: Swiss Webster / NIH.
[2]"Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta."
Aoyama C., Yamazaki N., Terada H., Ishidate K.
J. Lipid Res. 41:452-464(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
Strain: 129/Sv.
Tissue: Liver.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-371 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-150 (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain and Limb.
[6]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-168 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryonic eye, Embryonic head and Eye.
[7]"Deletion and alanine mutation analyses for the formation of active homo-or hetero-dimer complexes of mouse choline kinase-alpha and -beta."
Liao H., Aoyama C., Ishidate K., Teraoka H.
Biochim. Biophys. Acta 1761:111-120(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"Early embryonic lethality caused by disruption of the gene for choline kinase alpha, the first enzyme in phosphatidylcholine biosynthesis."
Wu G., Aoyama C., Young S.G., Vance D.E.
J. Biol. Chem. 283:1456-1462(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011002 mRNA. Translation: BAA24898.1.
AB030621 Genomic DNA. Translation: BAA88153.1.
AB030621 Genomic DNA. Translation: BAA88154.1. Sequence problems.
AC133523 Genomic DNA. No translation available.
CH466612 Genomic DNA. Translation: EDL32956.1.
BC056758 mRNA. Translation: AAH56758.1. Sequence problems.
BC060218 mRNA. No translation available.
AK014174 mRNA. Translation: BAB29191.1. Sequence problems.
AK052056 mRNA. Translation: BAC34841.1. Sequence problems.
AK053818 mRNA. Translation: BAC35539.1. Sequence problems.
CCDSCCDS29400.1. [O54804-2]
CCDS70913.1. [O54804-1]
RefSeqNP_001258425.1. NM_001271496.1. [O54804-1]
NP_038518.2. NM_013490.4. [O54804-2]
UniGeneMm.225505.

3D structure databases

ProteinModelPortalO54804.
SMRO54804. Positions 77-453.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO54804.

Proteomic databases

PaxDbO54804.
PRIDEO54804.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025760; ENSMUSP00000025760; ENSMUSG00000024843. [O54804-1]
ENSMUST00000072055; ENSMUSP00000071933; ENSMUSG00000024843. [O54804-2]
GeneID12660.
KEGGmmu:12660.

Organism-specific databases

CTD1119.
MGIMGI:107760. Chka.

Phylogenomic databases

eggNOGCOG0510.
GeneTreeENSGT00530000062991.
HOGENOMHOG000041274.
HOVERGENHBG050943.
InParanoidO54804.
KOK14156.
OMARAYLWCK.
TreeFamTF313549.

Enzyme and pathway databases

BRENDA2.7.1.82. 3474.
UniPathwayUPA00558; UER00741.
UPA00753; UER00737.

Gene expression databases

ArrayExpressO54804.
CleanExMM_CHKA.
GenevestigatorO54804.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCHKA. mouse.
NextBio281884.
PROO54804.
SOURCESearch...

Entry information

Entry nameCHKA_MOUSE
AccessionPrimary (citable) accession number: O54804
Secondary accession number(s): G5E853 expand/collapse secondary AC list , Q8BPL3, Q8BPW6, Q9CXP3, Q9QX56
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot