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O54790

- MAFG_MOUSE

UniProt

O54790 - MAFG_MOUSE

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Protein

Transcription factor MafG

Gene

Mafg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H+ (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. adult behavior Source: MGI
  2. in utero embryonic development Source: MGI
  3. regulation of cell proliferation Source: MGI
  4. regulation of cellular pH Source: Ensembl
  5. regulation of epidermal cell differentiation Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafG
Alternative name(s):
V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Gene namesi
Name:Mafg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:96911. Mafg.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice, although viable and fertile, exhibit abnormal megakaryocyte proliferation as well as age-dependent behavioral defects. Megakaryocytes display both anti-glycoprotein IIb immunoreactivity and anti-acetylcholinesterase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162Transcription factor MafGPRO_0000076501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki14 – 14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei53 – 531N6-acetyllysineCurated
Modified residuei60 – 601N6-acetyllysineCurated
Modified residuei71 – 711N6-acetyllysineCurated
Modified residuei76 – 761N6-acetyllysineCurated
Modified residuei124 – 1241PhosphoserineBy similarity

Post-translational modificationi

Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NF-E2.
Sumoylation at Lys-14 is required for active transcriptional repression.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO54790.
PaxDbiO54790.
PRIDEiO54790.

PTM databases

PhosphoSiteiO54790.

Expressioni

Developmental stagei

Expressed throughout the embryo up until E8.5 with strong expression in the neural tube. Expression continues throughout the embryo with some intense expression also in the epithelium of the intestine, skeletal muscle, lens, retina, cranial nerve, and dorsal root ganglion cells. After birth, strong expression in the epidermis, hair follicles, epithelium of the digestive and respiratory tracts, and kidney tubules.1 Publication

Gene expression databases

BgeeiO54790.
GenevestigatoriO54790.

Interactioni

Subunit structurei

Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with NFE2; the interaction results in transactivation activation. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding (By similarity).By similarity

Protein-protein interaction databases

BioGridi201283. 3 interactions.
DIPiDIP-46344N.
IntActiO54790. 1 interaction.
MINTiMINT-4101043.

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 316Combined sources
Helixi34 – 396Combined sources
Turni40 – 434Combined sources
Helixi46 – 8338Combined sources
Turni84 – 863Combined sources
Turni89 – 946Combined sources
Helixi99 – 1024Combined sources
Turni103 – 1053Combined sources
Beta strandi106 – 1094Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K1VNMR-A24-64[»]
3A5TX-ray2.80A/B21-123[»]
ProteinModelPortaliO54790.
SMRiO54790. Positions 21-111.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 11464bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 7624Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni79 – 9315Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG236209.
GeneTreeiENSGT00550000074549.
HOGENOMiHOG000045475.
HOVERGENiHBG001725.
InParanoidiO54790.
KOiK09037.
OMAiITASMGP.
OrthoDBiEOG7BGHMQ.
PhylomeDBiO54790.
TreeFamiTF325689.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR028551. MafG.
IPR008917. TF_DNA-bd.
IPR024874. Transciption_factor_Maf.
[Graphical view]
PANTHERiPTHR10129. PTHR10129. 1 hit.
PTHR10129:SF15. PTHR10129:SF15. 1 hit.
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54790-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEII
60 70 80 90 100
QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM
110 120 130 140 150
KLELDALRSK YEALQNFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV
160
ITIVKSKTDA RS
Length:162
Mass (Da):17,877
Last modified:June 1, 1998 - v1
Checksum:iC7FF19614EB95C7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009693 Genomic DNA. Translation: BAA24028.1.
BC002092 mRNA. Translation: AAH02092.1.
BC052633 mRNA. Translation: AAH52633.1.
CCDSiCCDS25748.1.
RefSeqiNP_034886.1. NM_010756.3.
UniGeneiMm.268010.
Mm.292957.
Mm.393664.

Genome annotation databases

EnsembliENSMUST00000058162; ENSMUSP00000053899; ENSMUSG00000051510.
ENSMUST00000106180; ENSMUSP00000101786; ENSMUSG00000051510.
ENSMUST00000106181; ENSMUSP00000101787; ENSMUSG00000051510.
ENSMUST00000106182; ENSMUSP00000101788; ENSMUSG00000051510.
GeneIDi17134.
KEGGimmu:17134.
UCSCiuc007mtu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009693 Genomic DNA. Translation: BAA24028.1 .
BC002092 mRNA. Translation: AAH02092.1 .
BC052633 mRNA. Translation: AAH52633.1 .
CCDSi CCDS25748.1.
RefSeqi NP_034886.1. NM_010756.3.
UniGenei Mm.268010.
Mm.292957.
Mm.393664.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K1V NMR - A 24-64 [» ]
3A5T X-ray 2.80 A/B 21-123 [» ]
ProteinModelPortali O54790.
SMRi O54790. Positions 21-111.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201283. 3 interactions.
DIPi DIP-46344N.
IntActi O54790. 1 interaction.
MINTi MINT-4101043.

PTM databases

PhosphoSitei O54790.

Proteomic databases

MaxQBi O54790.
PaxDbi O54790.
PRIDEi O54790.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000058162 ; ENSMUSP00000053899 ; ENSMUSG00000051510 .
ENSMUST00000106180 ; ENSMUSP00000101786 ; ENSMUSG00000051510 .
ENSMUST00000106181 ; ENSMUSP00000101787 ; ENSMUSG00000051510 .
ENSMUST00000106182 ; ENSMUSP00000101788 ; ENSMUSG00000051510 .
GeneIDi 17134.
KEGGi mmu:17134.
UCSCi uc007mtu.2. mouse.

Organism-specific databases

CTDi 4097.
MGIi MGI:96911. Mafg.

Phylogenomic databases

eggNOGi NOG236209.
GeneTreei ENSGT00550000074549.
HOGENOMi HOG000045475.
HOVERGENi HBG001725.
InParanoidi O54790.
KOi K09037.
OMAi ITASMGP.
OrthoDBi EOG7BGHMQ.
PhylomeDBi O54790.
TreeFami TF325689.

Enzyme and pathway databases

Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSi Mafg. mouse.
EvolutionaryTracei O54790.
NextBioi 291340.
PROi O54790.
SOURCEi Search...

Gene expression databases

Bgeei O54790.
Genevestigatori O54790.

Family and domain databases

Gene3Di 1.10.880.10. 1 hit.
InterProi IPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR028551. MafG.
IPR008917. TF_DNA-bd.
IPR024874. Transciption_factor_Maf.
[Graphical view ]
PANTHERi PTHR10129. PTHR10129. 1 hit.
PTHR10129:SF15. PTHR10129:SF15. 1 hit.
Pfami PF03131. bZIP_Maf. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
SUPFAMi SSF47454. SSF47454. 1 hit.
PROSITEi PS50217. BZIP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice."
    Shavit J.A., Motohashi H., Onodera K., Akasaka J., Yamamoto M., Engel J.D.
    Genes Dev. 12:2164-2174(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, FUNCTION.
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland and Olfactory epithelium.
  3. Cited for: SUMOYLATION AT LYS-14, FUNCTION.
  4. Cited for: STRUCTURE BY NMR OF 24-64.

Entry informationi

Entry nameiMAFG_MOUSE
AccessioniPrimary (citable) accession number: O54790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3