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O54790

- MAFG_MOUSE

UniProt

O54790 - MAFG_MOUSE

Protein

Transcription factor MafG

Gene

Mafg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H+ By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. adult behavior Source: MGI
    2. in utero embryonic development Source: MGI
    3. regulation of cell proliferation Source: MGI
    4. regulation of cellular pH Source: Ensembl
    5. regulation of epidermal cell differentiation Source: MGI
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor MafG
    Alternative name(s):
    V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
    Gene namesi
    Name:Mafg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:96911. Mafg.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice, although viable and fertile, exhibit abnormal megakaryocyte proliferation as well as age-dependent behavioral defects. Megakaryocytes display both anti-glycoprotein IIb immunoreactivity and anti-acetylcholinesterase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 162162Transcription factor MafGPRO_0000076501Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki14 – 14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Modified residuei53 – 531N6-acetyllysineCurated
    Modified residuei60 – 601N6-acetyllysineCurated
    Modified residuei71 – 711N6-acetyllysineCurated
    Modified residuei76 – 761N6-acetyllysineCurated
    Modified residuei124 – 1241PhosphoserineBy similarity

    Post-translational modificationi

    Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NF-E2.
    Sumoylation at Lys-14 is required for active transcriptional repression.1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiO54790.
    PRIDEiO54790.

    PTM databases

    PhosphoSiteiO54790.

    Expressioni

    Developmental stagei

    Expressed throughout the embryo up until E8.5 with strong expression in the neural tube. Expression continues throughout the embryo with some intense expression also in the epithelium of the intestine, skeletal muscle, lens, retina, cranial nerve, and dorsal root ganglion cells. After birth, strong expression in the epidermis, hair follicles, epithelium of the digestive and respiratory tracts, and kidney tubules.1 Publication

    Gene expression databases

    BgeeiO54790.
    GenevestigatoriO54790.

    Interactioni

    Subunit structurei

    Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with NFE2; the interaction results in transactivation activation. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201283. 3 interactions.
    DIPiDIP-46344N.
    IntActiO54790. 1 interaction.
    MINTiMINT-4101043.

    Structurei

    Secondary structure

    1
    162
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi26 – 316
    Helixi34 – 396
    Turni40 – 434
    Helixi46 – 8338
    Turni84 – 863
    Turni89 – 946
    Helixi99 – 1024
    Turni103 – 1053
    Beta strandi106 – 1094

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K1VNMR-A24-64[»]
    3A5TX-ray2.80A/B21-123[»]
    ProteinModelPortaliO54790.
    SMRiO54790. Positions 21-111.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO54790.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 11464bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 7624Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni79 – 9315Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. Maf subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG236209.
    GeneTreeiENSGT00550000074549.
    HOGENOMiHOG000045475.
    HOVERGENiHBG001725.
    InParanoidiO54790.
    KOiK09037.
    OMAiITASMGP.
    OrthoDBiEOG7BGHMQ.
    PhylomeDBiO54790.
    TreeFamiTF325689.

    Family and domain databases

    Gene3Di1.10.880.10. 1 hit.
    InterProiIPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR028551. MafG.
    IPR008917. TF_DNA-bd.
    IPR024874. Transciption_factor_Maf.
    [Graphical view]
    PANTHERiPTHR10129. PTHR10129. 1 hit.
    PTHR10129:SF15. PTHR10129:SF15. 1 hit.
    PfamiPF03131. bZIP_Maf. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47454. SSF47454. 1 hit.
    PROSITEiPS50217. BZIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54790-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEII    50
    QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM 100
    KLELDALRSK YEALQNFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV 150
    ITIVKSKTDA RS 162
    Length:162
    Mass (Da):17,877
    Last modified:June 1, 1998 - v1
    Checksum:iC7FF19614EB95C7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009693 Genomic DNA. Translation: BAA24028.1.
    BC002092 mRNA. Translation: AAH02092.1.
    BC052633 mRNA. Translation: AAH52633.1.
    CCDSiCCDS25748.1.
    RefSeqiNP_034886.1. NM_010756.3.
    UniGeneiMm.268010.
    Mm.292957.
    Mm.393664.

    Genome annotation databases

    EnsembliENSMUST00000058162; ENSMUSP00000053899; ENSMUSG00000051510.
    ENSMUST00000106180; ENSMUSP00000101786; ENSMUSG00000051510.
    ENSMUST00000106181; ENSMUSP00000101787; ENSMUSG00000051510.
    ENSMUST00000106182; ENSMUSP00000101788; ENSMUSG00000051510.
    GeneIDi17134.
    KEGGimmu:17134.
    UCSCiuc007mtu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009693 Genomic DNA. Translation: BAA24028.1 .
    BC002092 mRNA. Translation: AAH02092.1 .
    BC052633 mRNA. Translation: AAH52633.1 .
    CCDSi CCDS25748.1.
    RefSeqi NP_034886.1. NM_010756.3.
    UniGenei Mm.268010.
    Mm.292957.
    Mm.393664.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K1V NMR - A 24-64 [» ]
    3A5T X-ray 2.80 A/B 21-123 [» ]
    ProteinModelPortali O54790.
    SMRi O54790. Positions 21-111.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201283. 3 interactions.
    DIPi DIP-46344N.
    IntActi O54790. 1 interaction.
    MINTi MINT-4101043.

    PTM databases

    PhosphoSitei O54790.

    Proteomic databases

    PaxDbi O54790.
    PRIDEi O54790.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000058162 ; ENSMUSP00000053899 ; ENSMUSG00000051510 .
    ENSMUST00000106180 ; ENSMUSP00000101786 ; ENSMUSG00000051510 .
    ENSMUST00000106181 ; ENSMUSP00000101787 ; ENSMUSG00000051510 .
    ENSMUST00000106182 ; ENSMUSP00000101788 ; ENSMUSG00000051510 .
    GeneIDi 17134.
    KEGGi mmu:17134.
    UCSCi uc007mtu.2. mouse.

    Organism-specific databases

    CTDi 4097.
    MGIi MGI:96911. Mafg.

    Phylogenomic databases

    eggNOGi NOG236209.
    GeneTreei ENSGT00550000074549.
    HOGENOMi HOG000045475.
    HOVERGENi HBG001725.
    InParanoidi O54790.
    KOi K09037.
    OMAi ITASMGP.
    OrthoDBi EOG7BGHMQ.
    PhylomeDBi O54790.
    TreeFami TF325689.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    EvolutionaryTracei O54790.
    NextBioi 291340.
    PROi O54790.
    SOURCEi Search...

    Gene expression databases

    Bgeei O54790.
    Genevestigatori O54790.

    Family and domain databases

    Gene3Di 1.10.880.10. 1 hit.
    InterProi IPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR028551. MafG.
    IPR008917. TF_DNA-bd.
    IPR024874. Transciption_factor_Maf.
    [Graphical view ]
    PANTHERi PTHR10129. PTHR10129. 1 hit.
    PTHR10129:SF15. PTHR10129:SF15. 1 hit.
    Pfami PF03131. bZIP_Maf. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47454. SSF47454. 1 hit.
    PROSITEi PS50217. BZIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice."
      Shavit J.A., Motohashi H., Onodera K., Akasaka J., Yamamoto M., Engel J.D.
      Genes Dev. 12:2164-2174(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, FUNCTION.
      Strain: 129/SvJ.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland and Olfactory epithelium.
    3. Cited for: SUMOYLATION AT LYS-14, FUNCTION.
    4. Cited for: STRUCTURE BY NMR OF 24-64.

    Entry informationi

    Entry nameiMAFG_MOUSE
    AccessioniPrimary (citable) accession number: O54790
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3