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Protein

DNA fragmentation factor subunit beta

Gene

Dffb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Enzyme regulationi

Inhibited by DFFA (DFF45).

GO - Molecular functioni

  • deoxyribonuclease activity Source: GO_Central
  • DNA binding Source: MGI
  • enzyme binding Source: MGI
  • nuclease activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-211227. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA fragmentation factor subunit beta (EC:3.-.-.-)
Alternative name(s):
Caspase-activated deoxyribonuclease
Short name:
CAD
Short name:
Caspase-activated DNase
DNA fragmentation factor 40 kDa subunit
Short name:
DFF-40
Gene namesi
Name:Dffb
Synonyms:Cad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196287. Dffb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447141 – 344DNA fragmentation factor subunit betaAdd BLAST344

Proteomic databases

MaxQBiO54788.
PaxDbiO54788.
PRIDEiO54788.

PTM databases

PhosphoSitePlusiO54788.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029027.
CleanExiMM_DFFB.
GenevisibleiO54788. MM.

Interactioni

Subunit structurei

Heterodimer of DFFA and DFFB. Interacts with HIST1H1A (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DFFAO002738EBI-7365197,EBI-727171From a different organism.
DffaO547866EBI-7365197,EBI-1634519

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199222. 3 interactors.
IntActiO54788. 3 interactors.
MINTiMINT-142143.
STRINGi10090.ENSMUSP00000030893.

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 15Combined sources7
Beta strandi16 – 19Combined sources4
Beta strandi23 – 28Combined sources6
Helixi29 – 39Combined sources11
Beta strandi48 – 51Combined sources4
Turni52 – 55Combined sources4
Turni60 – 62Combined sources3
Beta strandi70 – 75Combined sources6
Helixi88 – 94Combined sources7
Helixi103 – 111Combined sources9
Helixi116 – 129Combined sources14
Turni138 – 140Combined sources3
Turni142 – 147Combined sources6
Helixi155 – 175Combined sources21
Helixi176 – 180Combined sources5
Turni183 – 185Combined sources3
Helixi186 – 202Combined sources17
Helixi203 – 210Combined sources8
Beta strandi212 – 214Combined sources3
Turni216 – 218Combined sources3
Beta strandi235 – 237Combined sources3
Helixi246 – 248Combined sources3
Helixi250 – 255Combined sources6
Turni256 – 258Combined sources3
Beta strandi259 – 265Combined sources7
Turni267 – 270Combined sources4
Helixi271 – 281Combined sources11
Helixi287 – 295Combined sources9
Turni297 – 299Combined sources3
Beta strandi300 – 303Combined sources4
Helixi305 – 307Combined sources3
Turni319 – 321Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9FNMR-A1-87[»]
1F2RNMR-C1-87[»]
1V0DX-ray2.60A1-329[»]
ProteinModelPortaliO54788.
SMRiO54788.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54788.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 83CIDE-NPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Contains 1 CIDE-N domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFTE. Eukaryota.
ENOG41102TS. LUCA.
GeneTreeiENSGT00390000014490.
HOGENOMiHOG000006502.
HOVERGENiHBG003828.
InParanoidiO54788.
KOiK02311.
OMAiESRFRNK.
OrthoDBiEOG091G0BE7.
PhylomeDBiO54788.
TreeFamiTF102022.

Family and domain databases

InterProiIPR015311. Apoptosis_DFF40.
IPR003508. CIDE-N_dom.
[Graphical view]
PfamiPF02017. CIDE-N. 1 hit.
PF09230. DFF40. 1 hit.
[Graphical view]
SMARTiSM00266. CAD. 1 hit.
[Graphical view]
PROSITEiPS51135. CIDE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAVLRQPKC VKLRALHSAC KFGVAARSCQ ELLRKGCVRF QLPMPGSRLC
60 70 80 90 100
LYEDGTEVTD DCFPGLPNDA ELLLLTAGET WHGYVSDITR FLSVFNEPHA
110 120 130 140 150
GVIQAARQLL SDEQAPLRQK LLADLLHHVS QNITAETREQ DPSWFEGLES
160 170 180 190 200
RFRNKSGYLR YSCESRIRGY LREVSAYTSM VDEAAQEEYL RVLGSMCQKL
210 220 230 240 250
KSVQYNGSYF DRGAEASSRL CTPEGWFSCQ GPFDLESCLS KHSINPYGNR
260 270 280 290 300
ESRILFSTWN LDHIIEKKRT VVPTLAEAIQ DGREVNWEYF YSLLFTAENL
310 320 330 340
KLVHIACHKK TTHKLECDRS RIYRPQTGSR RKQPARKKRP ARKR
Length:344
Mass (Da):39,449
Last modified:June 1, 1998 - v1
Checksum:iE854B413EA139DE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009377 mRNA. Translation: BAA24977.1.
CCDSiCCDS19004.1.
RefSeqiNP_031885.3. NM_007859.4.
UniGeneiMm.388918.

Genome annotation databases

EnsembliENSMUST00000030893; ENSMUSP00000030893; ENSMUSG00000029027.
GeneIDi13368.
KEGGimmu:13368.
UCSCiuc008wav.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009377 mRNA. Translation: BAA24977.1.
CCDSiCCDS19004.1.
RefSeqiNP_031885.3. NM_007859.4.
UniGeneiMm.388918.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9FNMR-A1-87[»]
1F2RNMR-C1-87[»]
1V0DX-ray2.60A1-329[»]
ProteinModelPortaliO54788.
SMRiO54788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199222. 3 interactors.
IntActiO54788. 3 interactors.
MINTiMINT-142143.
STRINGi10090.ENSMUSP00000030893.

PTM databases

PhosphoSitePlusiO54788.

Proteomic databases

MaxQBiO54788.
PaxDbiO54788.
PRIDEiO54788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030893; ENSMUSP00000030893; ENSMUSG00000029027.
GeneIDi13368.
KEGGimmu:13368.
UCSCiuc008wav.2. mouse.

Organism-specific databases

CTDi1677.
MGIiMGI:1196287. Dffb.

Phylogenomic databases

eggNOGiENOG410IFTE. Eukaryota.
ENOG41102TS. LUCA.
GeneTreeiENSGT00390000014490.
HOGENOMiHOG000006502.
HOVERGENiHBG003828.
InParanoidiO54788.
KOiK02311.
OMAiESRFRNK.
OrthoDBiEOG091G0BE7.
PhylomeDBiO54788.
TreeFamiTF102022.

Enzyme and pathway databases

ReactomeiR-MMU-211227. Activation of DNA fragmentation factor.

Miscellaneous databases

EvolutionaryTraceiO54788.
PROiO54788.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029027.
CleanExiMM_DFFB.
GenevisibleiO54788. MM.

Family and domain databases

InterProiIPR015311. Apoptosis_DFF40.
IPR003508. CIDE-N_dom.
[Graphical view]
PfamiPF02017. CIDE-N. 1 hit.
PF09230. DFF40. 1 hit.
[Graphical view]
SMARTiSM00266. CAD. 1 hit.
[Graphical view]
PROSITEiPS51135. CIDE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDFFB_MOUSE
AccessioniPrimary (citable) accession number: O54788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.