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Protein

DNA fragmentation factor subunit alpha

Gene

Dffa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of the caspase-activated DNase (DFF40).

GO - Molecular functioni

GO - Biological processi

  • chaperone-mediated protein folding Source: MGI
  • negative regulation of apoptotic DNA fragmentation Source: MGI
  • negative regulation of deoxyribonuclease activity Source: MGI
  • negative regulation of execution phase of apoptosis Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • thymocyte apoptotic process Source: MGI

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-211227 Activation of DNA fragmentation factor

Names & Taxonomyi

Protein namesi
Recommended name:
DNA fragmentation factor subunit alpha
Alternative name(s):
DNA fragmentation factor 45 kDa subunit
Short name:
DFF-45
Inhibitor of CAD
Short name:
ICAD
Gene namesi
Name:Dffa
Synonyms:Icad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196227 Dffa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447171 – 331DNA fragmentation factor subunit alphaAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei243PhosphothreonineBy similarity1

Post-translational modificationi

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei117 – 118Cleavage; by caspase-3By similarity2
Sitei224 – 225Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO54786
MaxQBiO54786
PaxDbiO54786
PeptideAtlasiO54786
PRIDEiO54786

PTM databases

iPTMnetiO54786
PhosphoSitePlusiO54786

Miscellaneous databases

PMAP-CutDBiQ8BQC7

Expressioni

Gene expression databases

BgeeiENSMUSG00000028974
CleanExiMM_DFFA
ExpressionAtlasiO54786 baseline and differential
GenevisibleiO54786 MM

Interactioni

Subunit structurei

Heterodimer of DFFA and DFFB.

Binary interactionsi

WithEntry#Exp.IntActNotes
DffbO547886EBI-1634519,EBI-7365197

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199211, 2 interactors
IntActiO54786, 2 interactors
MINTiO54786
STRINGi10090.ENSMUSP00000030816

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Beta strandi19 – 25Combined sources7
Beta strandi28 – 38Combined sources11
Helixi39 – 50Combined sources12
Helixi54 – 56Combined sources3
Beta strandi60 – 66Combined sources7
Beta strandi72 – 79Combined sources8
Beta strandi84 – 88Combined sources5
Beta strandi90 – 92Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F2RNMR-I1-100[»]
ProteinModelPortaliO54786
SMRiO54786
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54786

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 96CIDE-NPROSITE-ProRule annotationAdd BLAST80

Phylogenomic databases

eggNOGiENOG410IU5B Eukaryota
ENOG4111HKY LUCA
GeneTreeiENSGT00510000048128
HOGENOMiHOG000112204
HOVERGENiHBG000683
InParanoidiO54786
KOiK02310
OMAiCLLRRNH
OrthoDBiEOG091G110A
TreeFamiTF102021

Family and domain databases

InterProiView protein in InterPro
IPR003508 CIDE-N_dom
IPR027296 DFF-C
IPR017299 DNA_fragmentation_factor_asu
IPR015121 DNA_fragmentation_mid_dom
PANTHERiPTHR12306:SF16 PTHR12306:SF16, 1 hit
PfamiView protein in Pfam
PF02017 CIDE-N, 1 hit
PF09033 DFF-C, 1 hit
PIRSFiPIRSF037865 DFF_alpha, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD316494 DNA_fragmentation_C, 1 hit
SMARTiView protein in SMART
SM00266 CAD, 1 hit
SUPFAMiSSF81783 SSF81783, 1 hit
PROSITEiView protein in PROSITE
PS51135 CIDE_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform ICAD-L (identifier: O54786-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSRGASAP DPDDVRPLKP CLLRRNHSRD QHGVAASSLE ELRSKACELL
60 70 80 90 100
AIDKSLTPIT LVLAEDGTIV DDDDYFLCLP SNTKFVALAC NEKWIYNDSD
110 120 130 140 150
GGTAWVSQES FEADEPDSRA GVKWKNVARQ LKEDLSSIIL LSEEDLQALI
160 170 180 190 200
DIPCAELAQE LCQSCATVQG LQSTLQQVLD QREEARQSKQ LLELYLQALE
210 220 230 240 250
KEGNILSNQK ESKAALSEEL DAVDTGVGRE MASEVLLRSQ ILTTLKEKPA
260 270 280 290 300
PELSLSSQDL ESVSKEDPKA LAVALSWDIR KAETVQQACT TELALRLQQV
310 320 330
QSLHSLRNLS ARRSPLPGEP QRPKRAKRDS S
Length:331
Mass (Da):36,572
Last modified:July 27, 2011 - v2
Checksum:i4EA9314DD470040F
GO
Isoform ICAD-S (identifier: O54786-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     262-265: SVSK → VGKN
     266-331: Missing.

Show »
Length:265
Mass (Da):29,183
Checksum:i588DCABE1E987912
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95I → T in BAA24140 (PubMed:9422506).Curated1
Sequence conflicti95I → T in BAA24141 (PubMed:9422513).Curated1
Sequence conflicti95I → T in CAQ52098 (PubMed:19468303).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001087262 – 265SVSK → VGKN in isoform ICAD-S. Curated4
Alternative sequenceiVSP_001088266 – 331Missing in isoform ICAD-S. CuratedAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009375 mRNA Translation: BAA24140.1
AB009376 mRNA Translation: BAA24141.1
AK051011 mRNA Translation: BAC34496.1
AL611967 Genomic DNA Translation: CAM13988.1
CU210839 Genomic DNA Translation: CAQ52098.1
CCDSiCCDS18951.1 [O54786-1]
CCDS18952.1 [O54786-2]
RefSeqiNP_001020467.1, NM_001025296.2 [O54786-1]
UniGeneiMm.41433

Genome annotation databases

EnsembliENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974 [O54786-1]
ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974 [O54786-2]
GeneIDi13347
KEGGimmu:13347
UCSCiuc008vvr.2 mouse [O54786-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDFFA_MOUSE
AccessioniPrimary (citable) accession number: O54786
Secondary accession number(s): B2KFX0, O54787, Q8BQC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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