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O54786 (DFFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA fragmentation factor subunit alpha
Alternative name(s):
DNA fragmentation factor 45 kDa subunit
Short name=DFF-45
Inhibitor of CAD
Short name=ICAD
Gene names
Name:Dffa
Synonyms:Icad
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of the caspase-activated DNase (DFF40).

Subunit structure

Heterodimer of DFFA and DFFB.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor By similarity.

Sequence similarities

Contains 1 CIDE-N domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DffbO547886EBI-1634519,EBI-7365197

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform ICAD-L (identifier: O54786-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform ICAD-S (identifier: O54786-2)

The sequence of this isoform differs from the canonical sequence as follows:
     262-265: SVSK → VGKN
     266-331: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331DNA fragmentation factor subunit alpha
PRO_0000144717

Regions

Domain17 – 9680CIDE-N

Sites

Site117 – 1182Cleavage; by caspase-3 By similarity
Site224 – 2252Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence262 – 2654SVSK → VGKN in isoform ICAD-S.
VSP_001087
Alternative sequence266 – 33166Missing in isoform ICAD-S.
VSP_001088

Experimental info

Sequence conflict951I → T in BAA24140. Ref.1
Sequence conflict951I → T in BAA24141. Ref.2
Sequence conflict951I → T in CAQ52098. Ref.4

Secondary structure

.................. 331
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform ICAD-L [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4EA9314DD470040F

FASTA33136,572
        10         20         30         40         50         60 
MELSRGASAP DPDDVRPLKP CLLRRNHSRD QHGVAASSLE ELRSKACELL AIDKSLTPIT 

        70         80         90        100        110        120 
LVLAEDGTIV DDDDYFLCLP SNTKFVALAC NEKWIYNDSD GGTAWVSQES FEADEPDSRA 

       130        140        150        160        170        180 
GVKWKNVARQ LKEDLSSIIL LSEEDLQALI DIPCAELAQE LCQSCATVQG LQSTLQQVLD 

       190        200        210        220        230        240 
QREEARQSKQ LLELYLQALE KEGNILSNQK ESKAALSEEL DAVDTGVGRE MASEVLLRSQ 

       250        260        270        280        290        300 
ILTTLKEKPA PELSLSSQDL ESVSKEDPKA LAVALSWDIR KAETVQQACT TELALRLQQV 

       310        320        330 
QSLHSLRNLS ARRSPLPGEP QRPKRAKRDS S 

« Hide

Isoform ICAD-S [UniParc].

Checksum: 588DCABE1E987912
Show »

FASTA26529,183

References

« Hide 'large scale' references
[1]"A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD."
Enari M., Sakahira H., Yokoyama H., Okawa K., Iwamatsu A., Nagata S.
Nature 391:43-50(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis."
Sakahira H., Enari M., Nagata S.
Nature 391:96-99(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009375 mRNA. Translation: BAA24140.1.
AB009376 mRNA. Translation: BAA24141.1.
AK051011 mRNA. Translation: BAC34496.1.
AL611967 Genomic DNA. Translation: CAM13988.1.
CU210839 Genomic DNA. Translation: CAQ52098.1.
CCDSCCDS18951.1. [O54786-1]
CCDS18952.1. [O54786-2]
RefSeqNP_001020467.1. NM_001025296.2. [O54786-1]
UniGeneMm.41433.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F2RNMR-I1-100[»]
ProteinModelPortalO54786.
SMRO54786. Positions 1-100, 239-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199211. 2 interactions.
IntActO54786. 2 interactions.
MINTMINT-142152.

PTM databases

PhosphoSiteO54786.

Proteomic databases

MaxQBO54786.
PaxDbO54786.
PRIDEO54786.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974. [O54786-1]
ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974. [O54786-2]
GeneID13347.
KEGGmmu:13347.
UCSCuc008vvr.1. mouse. [O54786-1]

Organism-specific databases

CTD1676.
MGIMGI:1196227. Dffa.

Phylogenomic databases

eggNOGNOG40170.
GeneTreeENSGT00510000048128.
HOGENOMHOG000112204.
HOVERGENHBG000683.
InParanoidQ8BQC7.
KOK02310.
OMANEKWAYN.
OrthoDBEOG7JQBP8.
TreeFamTF102021.

Gene expression databases

BgeeO54786.
CleanExMM_DFFA.
GenevestigatorO54786.

Family and domain databases

Gene3D1.10.1490.10. 1 hit.
InterProIPR003508. CIDE-N_dom.
IPR027296. DFF-C_dom.
IPR017299. DNA_fragmentation_factor_asu.
IPR015121. DNA_fragmentation_mid_dom.
[Graphical view]
PfamPF02017. CIDE-N. 1 hit.
PF09033. DFF-C. 1 hit.
[Graphical view]
PIRSFPIRSF037865. DFF_alpha. 1 hit.
ProDomPD316494. DNA_fragmentation_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00266. CAD. 1 hit.
[Graphical view]
SUPFAMSSF81783. SSF81783. 1 hit.
PROSITEPS51135. CIDE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO54786.
NextBio283666.
PMAP-CutDBQ8BQC7.
PROO54786.
SOURCESearch...

Entry information

Entry nameDFFA_MOUSE
AccessionPrimary (citable) accession number: O54786
Secondary accession number(s): B2KFX0, O54787, Q8BQC7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot