LIM domain kinase 2 - Mus musculus (Mouse)

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O54785 (LIMK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
LIM domain kinase 2
Short name=LIMK-2
EC=2.7.11.1

Gene names

Name:

Limk2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Binds ROCK1 and LKAP. Interacts with PARD3 By similarity. Interacts with NISCH. Ref.6
Subcellular location	Cytoplasm.
Tissue specificity	Isoform 3 is testis specific.
Post-translational modification	Phosphorylated on serine and/or threonine residues by ROCK1 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	LIM domain Repeat
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spermatogenesis Inferred from mutant phenotype PubMed 11784110. Source: MGI
Cellular_component	cis-Golgi network Inferred from direct assay PubMed 16399995. Source: MGI cytoplasm Inferred from direct assay PubMed 16399995. Source: MGI nucleus Inferred from direct assay PubMed 16399995. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from mutant phenotype PubMed 11784110. Source: MGI protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O54785-1) Also known as: LIMK2a; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O54785-2) Also known as: LIMK2b; The sequence of this isoform differs from the canonical sequence as follows: 1-37: MAALAGDEAWRCRGCGTYVPLSQRLYRTANEAWHGSC → MGSYLSVPAYFTSRDP

Isoform 3 (identifier: O54785-3) Also known as: LIMK2t; tLIMK2; The sequence of this isoform differs from the canonical sequence as follows: 1-187: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 638

638

LIM domain kinase 2

PRO_0000075810

Regions

Domain

12 – 63

LIM zinc-binding 1

Domain

72 – 124

LIM zinc-binding 2

Domain

152 – 239

PDZ

Domain

331 – 608

278

Protein kinase

Nucleotide binding

337 – 345

ATP By similarity

Sites

Active site

451

By similarity

Binding site

360

ATP By similarity

Amino acid modifications

Modified residue

210

Phosphothreonine By similarity

Modified residue

293

Phosphoserine By similarity

Modified residue

298

Phosphoserine By similarity

Modified residue

505

Phosphothreonine; by ROCK1 and CDC42BP By similarity

Natural variations

Alternative sequence

1 – 187

187

Missing in isoform 3.

VSP_010351

Alternative sequence

1 – 37

MAALA…WHGSC → MGSYLSVPAYFTSRDP in isoform 2.

VSP_010350

Secondary structure

638

Helix Strand Turn

Details...

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (LIMK2a) [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 54CFA5E7A6A79C39 Show »	FASTA	638	72,202
10 20 30 40 50 60 MAALAGDEAW RCRGCGTYVP LSQRLYRTAN EAWHGSCFRC SECQESLTNW YYEKDGKLYC 70 80 90 100 110 120 HKDYWAKFGE FCHGCSLLMT GPAMVAGEFK YHPECFACMS CKVIIEDGDA YALVQHATLY 130 140 150 160 170 180 CGKCHNEVVL APMFERLSTE SVQDQLPYSV TLISMPATTE CRRGFSVTVE SASSNYATTV 190 200 210 220 230 240 QVKEVNRMHI SPNNRNAIHP GDRILEINGT PVRTLRVEEV EDAIKQTSQT LQLLIEHDPV 250 260 270 280 290 300 PQRLDQLRLD ARLPPHMQST GHTLMLSTLD TKENQEGTLR RRSLRRSNSI SKSPGPSSPK 310 320 330 340 350 360 EPLLLSRDIS RSESLRCSSS YSQQIFRPCD LIHGEVLGKG FFGQAIKVTH KATGKVMVMK 370 380 390 400 410 420 ELIRCDEETQ KTFLTEVKVM RSLDHPNVLK FIGVLYKDKK LNLLTEYIEG GTLKDFLRSV 430 440 450 460 470 480 DPFPWQQKVR FAKGISSGMA YLHSMCIIHR DLNSHNCLIK LDKTVVVADF GLSRLIVEER 490 500 510 520 530 540 KRPPVEKATT KKRTLRKSDR KKRYTVVGNP YWMAPEMLNG KSYDETVDVF SFGIVLCEII 550 560 570 580 590 600 GQVYADPDCL PRTLDFGLNV KLFWEKFVPT DCPPAFFPLA AICCKLEPES RPAFSKLEDS 610 620 630 FEALSLFLGE LAIPLPAELE DLDHTVSMEY GLTRDSPP « Hide
Isoform 2 (LIMK2b) [UniParc]. Checksum: 1E2DCE61958B8D0B Show »	FASTA	617	69,891
10 20 30 40 50 60 MGSYLSVPAY FTSRDPFRCS ECQESLTNWY YEKDGKLYCH KDYWAKFGEF CHGCSLLMTG 70 80 90 100 110 120 PAMVAGEFKY HPECFACMSC KVIIEDGDAY ALVQHATLYC GKCHNEVVLA PMFERLSTES 130 140 150 160 170 180 VQDQLPYSVT LISMPATTEC RRGFSVTVES ASSNYATTVQ VKEVNRMHIS PNNRNAIHPG 190 200 210 220 230 240 DRILEINGTP VRTLRVEEVE DAIKQTSQTL QLLIEHDPVP QRLDQLRLDA RLPPHMQSTG 250 260 270 280 290 300 HTLMLSTLDT KENQEGTLRR RSLRRSNSIS KSPGPSSPKE PLLLSRDISR SESLRCSSSY 310 320 330 340 350 360 SQQIFRPCDL IHGEVLGKGF FGQAIKVTHK ATGKVMVMKE LIRCDEETQK TFLTEVKVMR 370 380 390 400 410 420 SLDHPNVLKF IGVLYKDKKL NLLTEYIEGG TLKDFLRSVD PFPWQQKVRF AKGISSGMAY 430 440 450 460 470 480 LHSMCIIHRD LNSHNCLIKL DKTVVVADFG LSRLIVEERK RPPVEKATTK KRTLRKSDRK 490 500 510 520 530 540 KRYTVVGNPY WMAPEMLNGK SYDETVDVFS FGIVLCEIIG QVYADPDCLP RTLDFGLNVK 550 560 570 580 590 600 LFWEKFVPTD CPPAFFPLAA ICCKLEPESR PAFSKLEDSF EALSLFLGEL AIPLPAELED 610 LDHTVSMEYG LTRDSPP « Hide
Isoform 3 (LIMK2t) (tLIMK2) [UniParc]. Checksum: 883063D71E077C2B Show »	FASTA	451	51,173
10 20 30 40 50 60 MHISPNNRNA IHPGDRILEI NGTPVRTLRV EEVEDAIKQT SQTLQLLIEH DPVPQRLDQL 70 80 90 100 110 120 RLDARLPPHM QSTGHTLMLS TLDTKENQEG TLRRRSLRRS NSISKSPGPS SPKEPLLLSR 130 140 150 160 170 180 DISRSESLRC SSSYSQQIFR PCDLIHGEVL GKGFFGQAIK VTHKATGKVM VMKELIRCDE 190 200 210 220 230 240 ETQKTFLTEV KVMRSLDHPN VLKFIGVLYK DKKLNLLTEY IEGGTLKDFL RSVDPFPWQQ 250 260 270 280 290 300 KVRFAKGISS GMAYLHSMCI IHRDLNSHNC LIKLDKTVVV ADFGLSRLIV EERKRPPVEK 310 320 330 340 350 360 ATTKKRTLRK SDRKKRYTVV GNPYWMAPEM LNGKSYDETV DVFSFGIVLC EIIGQVYADP 370 380 390 400 410 420 DCLPRTLDFG LNVKLFWEKF VPTDCPPAFF PLAAICCKLE PESRPAFSKL EDSFEALSLF 430 440 450 LGELAIPLPA ELEDLDHTVS MEYGLTRDSP P « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, genomic organization, and chromosomal localization of the mouse LIM motif-containing kinase gene, Limk2." Koshimizu U., Takahashi H., Yoshida M.C., Nakamura T. Biochem. Biophys. Res. Commun. 241:243-250(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: ICR. Tissue: Embryo.
[2]	"Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and tissue-specific expression of two alternatively initiated transcripts." Ikebe C., Ohashi K., Fujimori T., Bernard O., Noda T., Robertson E.J., Mizuno K. Genomics 46:504-508(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2). Strain: 129/Sv. Tissue: Kidney.
[3]	"A novel transcript encoding truncated LIM kinase 2 is specifically expressed in male germ cells undergoing meiosis." Takahashi H., Koshimizu U., Nakamura T. Biochem. Biophys. Res. Commun. 249:138-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"Identification of testis-specific (LimK2t) and brain-specific (LimK2c) isoforms of mouse LIM-kinase 2 gene transcripts." Ikebe C., Ohashi K., Mizuno K. Biochem. Biophys. Res. Commun. 246:307-312(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary tumor.
[6]	"Nischarin inhibits LIM kinase to regulate cofilin phosphorylation and cell invasion." Ding Y., Milosavljevic T., Alahari S.K. Mol. Cell. Biol. 28:3742-3756(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NISCH.
[7]	"Solution structure of the PDZ domain from mouse LIM domain kinase." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 139-246.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB008117 mRNA. Translation: BAA29035.1.
AB005140 Genomic DNA. Translation: BAA24491.1.
AB005131 mRNA. Translation: BAA24488.1.
AB005132 mRNA. Translation: BAA24489.1.
AB005134 Genomic DNA. Translation: BAA24490.1.
U88618 mRNA. Translation: AAC39947.1.
AB012291 mRNA. Translation: BAA32437.1.
AB012092 mRNA. Translation: BAA31147.1.
BC007129 mRNA. Translation: AAH07129.1.

IPI

IPI00283633.
IPI00417118.
IPI00469661.

PIR

JC5813.
JC5814.
JE0240.

RefSeq

NP_001029202.1. NM_001034030.1.
NP_034848.1. NM_010718.3.
NP_774958.1. NM_173053.1.

UniGene

Mm.124176.
Mm.390323.
Mm.442189.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YUB	NMR	-	A	142-246	[»]

ProteinModelPortal

O54785.

SMR

O54785. Positions 9-132, 139-246, 276-623.

ModBase

Search...

PTM databases

PhosphoSite

O54785.

Proteomic databases

PRIDE

O54785.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000101638; ENSMUSP00000099162; ENSMUSG00000020451.
ENSMUST00000101642; ENSMUSP00000099165; ENSMUSG00000020451.
ENSMUST00000110029; ENSMUSP00000105656; ENSMUSG00000020451.

GeneID

16886.

KEGG

mmu:16886.

Organism-specific databases

CTD

3985.

MGI

MGI:1197517. Limk2.

Phylogenomic databases

eggNOG

COG0515.

GeneTree

ENSGT00530000063025.

HOGENOM

HOG000013121.

HOVERGEN

HBG052328.

InParanoid

O54785.

K05744.

OMA

DARLSPH.

OrthoDB

EOG4C87RX.

Gene expression databases

ArrayExpress

O54785.

Bgee

O54785.

CleanEx

MM_LIMK2.

Genevestigator

O54785.

GermOnline

ENSMUSG00000020451. Mus musculus.

Family and domain databases

Gene3D

2.10.110.10. 2 hits.

InterPro

IPR011009. Kinase-like_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00412. LIM. 2 hits.
PF00595. PDZ. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]

SMART

SM00132. LIM. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.
SSF50156. PDZ. 1 hit.

PROSITE

PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
PS50106. PDZ. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O54785.

NextBio

290896.

SOURCE

Search...

Entry information

Entry name

LIMK2_MOUSE

Accession

Primary (citable) accession number: O54785
Secondary accession number(s): O54776, O55238, Q9QUL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents