Reviewed, UniProtKB/Swiss-Prot O54783 (CHKB_RAT)

Names and origin

Protein names

Recommended name:
    Choline/ethanolamine kinase
Alternative name(s):
    choline/ethanolamine kinase beta
      Short name=CKEKB
Including the following 2 domains:
    1- Recommended name:
            Choline kinase beta
                Short name=CKB
                Short name=CK
              EC=2.7.1.32
    2- Recommended name:
            Ethanolamine kinase
                Short name=EK
              EC=2.7.1.82

Gene names

Name:	Chkb
Synonyms:	Chetk, Chkl

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + choline = ADP + O-phosphocholine. ATP + ethanolamine = ADP + O-phosphoethanolamine.
Sequence similarities	Belongs to the choline/ethanolamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW choline kinase activity Ref.1 Inferred from direct assay. Source: RGD ethanolamine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 394	393	Choline/ethanolamine kinase		PRO_0000206224
Sites
	Active site	240	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	39	1	Phosphoserine By similarity
	Modified residue	42	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54783-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A2E99EB055E3959E	FASTA	394	45,100
	10 20 30 40 50 60 MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY QWCREYLGGA 70 80 90 100 110 120 WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE VLLRLYGAIL QGVDSLVLES 130 140 150 160 170 180 VMFAILAERS LGPQLYGVFP EGRLEQYLPS RPLKTQELRD PVLSGAIATK MARFHGMEMP 190 200 210 220 230 240 FTKEPRWLFG TMERYLKQIQ DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH 250 260 270 280 290 300 NDIQEGNILL LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 310 320 330 340 350 360 ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH FFWGLWSTLQ 370 380 390 ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase." Aoyama C., Nakashima K., Matsui M., Ishidate K. Biochim. Biophys. Acta 1390:1-7(1998) [PubMed: 9487136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006607 mRNA. Translation: BAA24366.1. BC060515 mRNA. Translation: AAH60515.1.
IPI	IPI00198119.
RefSeq	NP_058873.1.
UniGene	Rn.1302
3D structure databases
SMR	O54783. Positions 41-389, 42-390.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000011404. Rattus norvegicus. [Contig view]
GeneID	29367.
KEGG	rno:29367.
Organism-specific databases
RGD	61826. Chkb.
Phylogenomic databases
HOVERGEN	O54783.
OMA	O54783. GAWRRVQ.
Enzyme and pathway databases
BRENDA	2.7.1.32. 248. 2.7.1.82. 248.
Gene expression databases
ArrayExpress	O54783.
GermOnline	ENSRNOG00000011404. Rattus norvegicus.
Family and domain databases
InterPro	IPR002573. Choline/ethanolamine_kinase. [Graphical view]
PANTHER	PTHR22603. Choline/ethanolamine_kinase. 1 hit.
Pfam	PF01633. Choline_kinase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608914.

Entry information

Entry name

CHKB_RAT

Accession

Primary (citable) accession number: O54783

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)