O54783 (CHKB_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline/ethanolamine kinase

Alternative name(s):
Choline kinase beta
Short name=CK
Short name=CKB
EC=2.7.1.32
Ethanolamine kinase
Short name=EK
EC=2.7.1.82
choline/ethanolamine kinase beta
Short name=CKEKB

Gene names

Name:	Chkb
Synonyms:	Chetk, Chkl

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis By similarity.
Catalytic activity	ATP + choline = ADP + O-phosphocholine. ATP + ethanolamine = ADP + O-phosphoethanolamine.
Pathway	Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
Subunit structure	Homodimer, and heterodimer with CHKA By similarity.
Sequence similarities	Belongs to the choline/ethanolamine kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylethanolamine biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW choline kinase activity Inferred from sequence or structural similarity. Source: UniProtKB ethanolamine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 394	393	Choline/ethanolamine kinase		PRO_0000206224
Regions
	Nucleotide binding	75 – 81	7	ATP By similarity
	Nucleotide binding	146 – 152	7	ATP By similarity
	Region	77 – 79	3	Substrate binding By similarity
Sites
	Binding site	104	1	ATP By similarity
	Binding site	244	1	ATP By similarity
	Binding site	264	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	39	1	Phosphoserine By similarity
	Modified residue	42	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54783 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A2E99EB055E3959E	FASTA	394	45,100
	10 20 30 40 50 60 MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY QWCREYLGGA 70 80 90 100 110 120 WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE VLLRLYGAIL QGVDSLVLES 130 140 150 160 170 180 VMFAILAERS LGPQLYGVFP EGRLEQYLPS RPLKTQELRD PVLSGAIATK MARFHGMEMP 190 200 210 220 230 240 FTKEPRWLFG TMERYLKQIQ DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH 250 260 270 280 290 300 NDIQEGNILL LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 310 320 330 340 350 360 ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH FFWGLWSTLQ 370 380 390 ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase." Aoyama C., Nakashima K., Matsui M., Ishidate K. Biochim. Biophys. Acta 1390:1-7(1998) [PubMed: 9487136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006607 mRNA. Translation: BAA24366.1. BC060515 mRNA. Translation: AAH60515.1.
IPI	IPI00198119.
RefSeq	NP_058873.1. NM_017177.1.
UniGene	Rn.1302.
3D structure databases
ProteinModelPortal	O54783.
SMR	O54783. Positions 42-390.
ModBase	Search...
Protein-protein interaction databases
STRING	O54783.
Proteomic databases
PRIDE	O54783.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404.
GeneID	29367.
KEGG	rno:29367.
UCSC	NM_017177. rat.
Organism-specific databases
CTD	1120.
RGD	61826. Chkb.
Phylogenomic databases
eggNOG	roNOG08629.
GeneTree	ENSGT00530000062991.
HOVERGEN	HBG050943.
InParanoid	O54783.
OMA	EMNLLEM.
OrthoDB	EOG49GKH0.
PhylomeDB	O54783.
Gene expression databases
ArrayExpress	O54783.
Genevestigator	O54783.
GermOnline	ENSRNOG00000011404. Rattus norvegicus.
Family and domain databases
InterPro	IPR002573. Choline/ethanolamine_kinase. IPR011009. Kinase-like_dom. [Graphical view]
KO	K14156.
Pfam	PF01633. Choline_kinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...
Other
NextBio	608914.

Entry information

Entry name

CHKB_RAT

Accession

Primary (citable) accession number: O54783

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program