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Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041ATPBy similarity
Binding sitei244 – 2441ATPBy similarity
Binding sitei264 – 2641ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 817ATPBy similarity
Nucleotide bindingi146 – 1527ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. choline kinase activity Source: UniProtKB
  3. ethanolamine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. CDP-choline pathway Source: GOC
  2. muscle organ development Source: Ensembl
  3. phosphatidylethanolamine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_284894. Synthesis of PE.
REACT_351400. Synthesis of PC.
SABIO-RKO54783.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta (EC:2.7.1.32)
Short name:
CK
Short name:
CKB
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
choline/ethanolamine kinase beta
Short name:
CKEKB
Gene namesi
Name:Chkb
Synonyms:Chetk, Chkl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi61826. Chkb.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 394393Choline/ethanolamine kinasePRO_0000206224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO54783.
PRIDEiO54783.

Expressioni

Gene expression databases

GenevestigatoriO54783.

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKA.By similarity

Protein-protein interaction databases

BioGridi248021. 1 interaction.
STRINGi10116.ENSRNOP00000015374.

Structurei

3D structure databases

ProteinModelPortaliO54783.
SMRiO54783. Positions 42-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 793Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiO54783.
KOiK14156.
OMAiIEMYLTR.
OrthoDBiEOG72VH68.
PhylomeDBiO54783.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY
60 70 80 90 100
QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE
110 120 130 140 150
VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS
160 170 180 190 200
RPLKTQELRD PVLSGAIATK MARFHGMEMP FTKEPRWLFG TMERYLKQIQ
210 220 230 240 250
DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH NDIQEGNILL
260 270 280 290 300
LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP
310 320 330 340 350
ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH
360 370 380 390
FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP
Length:394
Mass (Da):45,100
Last modified:January 23, 2007 - v3
Checksum:iA2E99EB055E3959E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006607 mRNA. Translation: BAA24366.1.
BC060515 mRNA. Translation: AAH60515.1.
RefSeqiNP_058873.1. NM_017177.1.
UniGeneiRn.1302.

Genome annotation databases

EnsembliENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404.
GeneIDi29367.
KEGGirno:29367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006607 mRNA. Translation: BAA24366.1.
BC060515 mRNA. Translation: AAH60515.1.
RefSeqiNP_058873.1. NM_017177.1.
UniGeneiRn.1302.

3D structure databases

ProteinModelPortaliO54783.
SMRiO54783. Positions 42-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248021. 1 interaction.
STRINGi10116.ENSRNOP00000015374.

Proteomic databases

PaxDbiO54783.
PRIDEiO54783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404.
GeneIDi29367.
KEGGirno:29367.

Organism-specific databases

CTDi1120.
RGDi61826. Chkb.

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiO54783.
KOiK14156.
OMAiIEMYLTR.
OrthoDBiEOG72VH68.
PhylomeDBiO54783.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
ReactomeiREACT_284894. Synthesis of PE.
REACT_351400. Synthesis of PC.
SABIO-RKO54783.

Miscellaneous databases

NextBioi608914.
PROiO54783.

Gene expression databases

GenevestigatoriO54783.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase."
    Aoyama C., Nakashima K., Matsui M., Ishidate K.
    Biochim. Biophys. Acta 1390:1-7(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiCHKB_RAT
AccessioniPrimary (citable) accession number: O54783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.