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O54783

- CHKB_RAT

UniProt

O54783 - CHKB_RAT

Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis By similarity.By similarity

    Catalytic activityi

    ATP + choline = ADP + phosphocholine.
    ATP + ethanolamine = ADP + O-phosphoethanolamine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041ATPBy similarity
    Binding sitei244 – 2441ATPBy similarity
    Binding sitei264 – 2641ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 817ATPBy similarity
    Nucleotide bindingi146 – 1527ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. choline kinase activity Source: UniProtKB
    3. ethanolamine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. CDP-choline pathway Source: GOC
    2. muscle organ development Source: Ensembl
    3. phosphatidylethanolamine biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_197258. Synthesis of PC.
    REACT_199141. Synthesis of PE.
    SABIO-RKO54783.
    UniPathwayiUPA00558; UER00741.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline/ethanolamine kinase
    Alternative name(s):
    Choline kinase beta (EC:2.7.1.32)
    Short name:
    CK
    Short name:
    CKB
    Ethanolamine kinase (EC:2.7.1.82)
    Short name:
    EK
    choline/ethanolamine kinase beta
    Short name:
    CKEKB
    Gene namesi
    Name:Chkb
    Synonyms:Chetk, Chkl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi61826. Chkb.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 394393Choline/ethanolamine kinasePRO_0000206224Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiO54783.
    PRIDEiO54783.

    Expressioni

    Gene expression databases

    GenevestigatoriO54783.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with CHKA.By similarity

    Protein-protein interaction databases

    BioGridi248021. 1 interaction.
    STRINGi10116.ENSRNOP00000015374.

    Structurei

    3D structure databases

    ProteinModelPortaliO54783.
    SMRiO54783. Positions 42-390.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 793Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0510.
    GeneTreeiENSGT00530000062991.
    HOGENOMiHOG000041274.
    HOVERGENiHBG050943.
    InParanoidiO54783.
    KOiK14156.
    OMAiFTKEPRW.
    OrthoDBiEOG72VH68.
    PhylomeDBiO54783.
    TreeFamiTF313549.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54783-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY    50
    QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE 100
    VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS 150
    RPLKTQELRD PVLSGAIATK MARFHGMEMP FTKEPRWLFG TMERYLKQIQ 200
    DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH NDIQEGNILL 250
    LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 300
    ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH 350
    FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP 394
    Length:394
    Mass (Da):45,100
    Last modified:January 23, 2007 - v3
    Checksum:iA2E99EB055E3959E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006607 mRNA. Translation: BAA24366.1.
    BC060515 mRNA. Translation: AAH60515.1.
    RefSeqiNP_058873.1. NM_017177.1.
    UniGeneiRn.1302.

    Genome annotation databases

    EnsembliENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404.
    GeneIDi29367.
    KEGGirno:29367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006607 mRNA. Translation: BAA24366.1 .
    BC060515 mRNA. Translation: AAH60515.1 .
    RefSeqi NP_058873.1. NM_017177.1.
    UniGenei Rn.1302.

    3D structure databases

    ProteinModelPortali O54783.
    SMRi O54783. Positions 42-390.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248021. 1 interaction.
    STRINGi 10116.ENSRNOP00000015374.

    Proteomic databases

    PaxDbi O54783.
    PRIDEi O54783.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015374 ; ENSRNOP00000015374 ; ENSRNOG00000011404 .
    GeneIDi 29367.
    KEGGi rno:29367.

    Organism-specific databases

    CTDi 1120.
    RGDi 61826. Chkb.

    Phylogenomic databases

    eggNOGi COG0510.
    GeneTreei ENSGT00530000062991.
    HOGENOMi HOG000041274.
    HOVERGENi HBG050943.
    InParanoidi O54783.
    KOi K14156.
    OMAi FTKEPRW.
    OrthoDBi EOG72VH68.
    PhylomeDBi O54783.
    TreeFami TF313549.

    Enzyme and pathway databases

    UniPathwayi UPA00558 ; UER00741 .
    Reactomei REACT_197258. Synthesis of PC.
    REACT_199141. Synthesis of PE.
    SABIO-RK O54783.

    Miscellaneous databases

    NextBioi 608914.
    PROi O54783.

    Gene expression databases

    Genevestigatori O54783.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase."
      Aoyama C., Nakashima K., Matsui M., Ishidate K.
      Biochim. Biophys. Acta 1390:1-7(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiCHKB_RAT
    AccessioniPrimary (citable) accession number: O54783
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3