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O54783 (CHKB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta
Short name=CK
Short name=CKB
EC=2.7.1.32
Ethanolamine kinase
Short name=EK
EC=2.7.1.82
choline/ethanolamine kinase beta
Short name=CKEKB
Gene names
Name:Chkb
Synonyms:Chetk, Chkl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis By similarity.

Catalytic activity

ATP + choline = ADP + phosphocholine.

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathway

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Subunit structure

Homodimer, and heterodimer with CHKA By similarity.

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 394393Choline/ethanolamine kinase
PRO_0000206224

Regions

Nucleotide binding75 – 817ATP By similarity
Nucleotide binding146 – 1527ATP By similarity
Region77 – 793Substrate binding By similarity

Sites

Binding site1041ATP By similarity
Binding site2441ATP By similarity
Binding site2641ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
O54783 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A2E99EB055E3959E

FASTA39445,100
        10         20         30         40         50         60 
MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY QWCREYLGGA 

        70         80         90        100        110        120 
WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE VLLRLYGAIL QGVDSLVLES 

       130        140        150        160        170        180 
VMFAILAERS LGPQLYGVFP EGRLEQYLPS RPLKTQELRD PVLSGAIATK MARFHGMEMP 

       190        200        210        220        230        240 
FTKEPRWLFG TMERYLKQIQ DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH 

       250        260        270        280        290        300 
NDIQEGNILL LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 

       310        320        330        340        350        360 
ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH FFWGLWSTLQ 

       370        380        390 
ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP

« Hide

References

« Hide 'large scale' references
[1]"Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase."
Aoyama C., Nakashima K., Matsui M., Ishidate K.
Biochim. Biophys. Acta 1390:1-7(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB006607 mRNA. Translation: BAA24366.1.
BC060515 mRNA. Translation: AAH60515.1.
IPIIPI00198119.
RefSeqNP_058873.1. NM_017177.1.
UniGeneRn.1302.

3D structure databases

ProteinModelPortalO54783.
SMRO54783. Positions 42-390.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000015374.

Proteomic databases

PaxDbO54783.
PRIDEO54783.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404.
GeneID29367.
KEGGrno:29367.

Organism-specific databases

CTD1120.
RGD61826. Chkb.

Phylogenomic databases

eggNOGCOG0510.
GeneTreeENSGT00530000062991.
HOGENOMHOG000041274.
HOVERGENHBG050943.
InParanoidO54783.
KOK14156.
OMAFTKEPRW.
OrthoDBEOG49GKH0.

Enzyme and pathway databases

SABIO-RKO54783.
UniPathwayUPA00558; UER00741.

Gene expression databases

GenevestigatorO54783.
GermOnlineENSRNOG00000011404. Rattus norvegicus.

Family and domain databases

InterProIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR22603. PTHR22603. 1 hit.
SUPFAMSSF56112. Kinase_like. 1 hit.
ProtoNetSearch...

Other

NextBio608914.

Entry information

Entry nameCHKB_RAT
AccessionPrimary (citable) accession number: O54783
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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