Epididymis-specific alpha-mannosidase precursor - Mus musculus (Mouse)

Contribute

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O54782 (MA2B2_MOUSE)

Last modified November 3, 2009. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Epididymis-specific alpha-mannosidase
    EC=3.2.1.24
Alternative name(s):
    Mannosidase alpha class 2B member 2

Gene names

Name:	Man2b2
Synonyms:	Kiaa0935

Organism

Mus musculus (Mouse)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1018 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an important role in the early step of spermatogenesis.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Mainly expressed in the narrow region between the caput and corpus epididymis.
Developmental stage	Specifically expressed in type A spermatogonia at stages IX-XI of spermatogenesis. Detected there until the cell developed into type B spermatogonia.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	mannose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-mannosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Potential

Chain

997

Epididymis-specific alpha-mannosidase

PRO_0000012078

Sites

Active site

1

Nucleophile By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1

S → T in BAA24266. Ref.1

Sequence conflict

1

M → I in BAB23655. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

O54782-1 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 41AD7AAEA7CC88A9
Show »

1,018

115,610

        10         20         30         40         50         60 
MGPLRWLPLL GQLLLLWPRA AQPAGPIRAF VVPHSHMDVG WVFTVQESMR AYAANVYTTV 

        70         80         90        100        110        120 
VAELVRGGQR RFIAVEQEFF RLWWDGVASE QQKQQVRQLL HEGRLEFVLG GQVMHDEAVT 

       130        140        150        160        170        180 
HLDDQILQLT EGHGFLYETF GIRPQFSWHV DPFGASATTP TLFALAGFNA HLISRIDYDL 

       190        200        210        220        230        240 
KDAMQEAQML QFVWHGSPSL SGQQEIFTHV MDHYSYCTPS HIPFSNRSGF YWNGVAVFPE 

       250        260        270        280        290        300 
PPPDGVYPNM SEPVTGANIH LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFD 

       310        320        330        340        350        360 
NMDPLLDYIN QRTAQFGISV QYATLNDYFQ ALHATNMTWG IRDHQDFLPY SSEPLQAWTG 

       370        380        390        400        410        420 
FYTSRSTLKG LARQASALLY AGESMFTRYM WPDPSGTLDP TWALQQLQQL RWAVSEVQHH 

       430        440        450        460        470        480 
DAITGTESPK VKNMYTEHLR MGMLGVRKLM VSIALGGPPG SGTGAPKDIM GPQVTPVLSV 

       490        500        510        520        530        540 
DTRPVGYSAS VYNPLAWKIT TIITLTVAFP NVSVTDELGH PVSTQIQNST KDPSAYDLLI 

       550        560        570        580        590        600 
LTTIPGLNYR HYQVMHARGD QAGTRELVAP RANTLKFSLK LRNQPSQEGK RLVPVMNDCY 

       610        620        630        640        650        660 
ILLFDQDTNM LHSIQDRQSN RTVRMTQEFL EYQANWDVKQ GPISDNYLFA PNNTAEPSWE 

       670        680        690        700        710        720 
AVGMEMVAGT LVTDIRQYFY RYITDQEYIY SIHTRLAHPS LAGELLCQRI EQQYRVGPLD 

       730        740        750        760        770        780 
LNREAILRTS SDLNSQQVLY SDNNGYQMQR RPYKAFKSNP IPRNYYPMVQ SAFIEDDKSR 

       790        800        810        820        830        840 
LVLLAERPHG VSSQGNGQVE VMLHRRLWNN LAWDLKYNLT LNDTSIVHPV LWLMLGPKST 

       850        860        870        880        890        900 
MTALHPRSGV ALQHGPVVLL KELADEETPV HGPHNPWPVT LPPNLHLQIL SVPGWTYSRS 

       910        920        930        940        950        960 
HAQHLRNLQR GHPEKPQANL QRVLLRLRHL YEAGEDPVLS RPATVDLKVV LRGLGSVVAV 

       970        980        990       1000       1010 
EERSLTGTWD VQMLQRWHWS TKTDHLKGHP TSPPRPPGGS IITVYPKEIR TFFIKFQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia." Hiramoto S., Tamba M., Kiuchi S., Jin Y.-Z., Bannai S., Sugita Y., Dacheux F., Dacheux J.-L., Yoshida M., Okamura N. Biochem. Biophys. Res. Commun. 241:439-445(1997) [PubMed: 9425289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ddY. Tissue: Testis.
[2]	"Locarization of a mouse homologue of the porcine 135kDa a-D-mannosidase, mMAN2B2, in acrosome and its involvement in the sperm-egg interaction." Hiramoto S., Noguchi J., Endoh Y., Tamba M., Okamura N. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CD-1. Tissue: Neural stem cell.
[5]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 763-1018. Tissue: Embryonic tail.
+	Additional computationally mapped references.

Cross-references

Sequence databases
	AB006458 mRNA. Translation: BAA24266.1. AB083124 Genomic DNA. Translation: BAC53862.1. AK004900 mRNA. Translation: BAB23655.1. AK040388 mRNA. Translation: BAC30580.1. BC066211 mRNA. Translation: AAH66211.1. AK173067 mRNA. Translation: BAD32345.1.
IPI	IPI00117842.
PIR	JC5799.
RefSeq	NP_032576.2.
UniGene	Mm.761
3D structure databases
HSSP	HSSP built from PDB template 1O7D based on UniProtKB Q29451.
ModBase	Search...
Protein-protein interaction databases
STRING	O54782.
Protein family/group databases
CAZy	GH38. Glycoside Hydrolase Family 38.
Proteomic databases
PRIDE	O54782.
Genome annotation databases
Ensembl	ENSMUST00000031002; ENSMUSP00000031002; ENSMUSG00000029119; Mus musculus. [Genome view]
GeneID	17160.
KEGG	mmu:17160.
Organism-specific databases
CTD	17160.
MGI	MGI:1195262. Man2b2.
Rouge	Search...
Phylogenomic databases
HOGENOM	O54782.
HOVERGEN	O54782.
OMA	EIRTFFV.
Enzyme and pathway databases
BRENDA	3.2.1.24. 244.
Gene expression databases
ArrayExpress	O54782.
Bgee	O54782.
CleanEx	MM_MAN2B2.
Genevestigator	O54782.
GermOnline	ENSMUSG00000029119. Mus musculus.
Family and domain databases
InterPro	IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_central. IPR000602. Glyco_hydro_38_core. [Graphical view]
Gene3D	G3DSA:3.20.110.10. Glyco_hydro_38_core. 1 hit.
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	291434.
SOURCE	Search...

Entry information

Entry name

MA2B2_MOUSE

Accession

Primary (citable) accession number: O54782
Secondary accession number(s): Q69ZV1, Q8BH85, Q9DBK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents