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O54782

- MA2B2_MOUSE

UniProt

O54782 - MA2B2_MOUSE

Protein

Epididymis-specific alpha-mannosidase

Gene

Man2b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Plays an important role in the early step of spermatogenesis.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361ZincBy similarity
    Metal bindingi38 – 381ZincBy similarity
    Active sitei151 – 1511NucleophileBy similarity
    Metal bindingi151 – 1511ZincBy similarity
    Metal bindingi420 – 4201ZincBy similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mannose metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
    Alternative name(s):
    Mannosidase alpha class 2B member 2
    Gene namesi
    Name:Man2b2
    Synonyms:Kiaa0935
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1195262. Man2b2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. lysosome Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 1018997Epididymis-specific alpha-mannosidasePRO_0000012078Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi511 – 5111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi818 – 8181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi822 – 8221N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiO54782.
    PaxDbiO54782.
    PRIDEiO54782.

    PTM databases

    PhosphoSiteiO54782.

    Expressioni

    Tissue specificityi

    Mainly expressed in the narrow region between the caput and corpus epididymis.

    Developmental stagei

    Specifically expressed in type A spermatogonia at stages IX-XI of spermatogenesis. Detected there until the cell developed into type B spermatogonia.

    Gene expression databases

    ArrayExpressiO54782.
    BgeeiO54782.
    CleanExiMM_MAN2B2.
    GenevestigatoriO54782.

    Interactioni

    Protein-protein interaction databases

    IntActiO54782. 1 interaction.
    MINTiMINT-4100949.

    Structurei

    3D structure databases

    ProteinModelPortaliO54782.
    SMRiO54782. Positions 27-807.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG306356.
    GeneTreeiENSGT00510000046304.
    HOGENOMiHOG000113450.
    HOVERGENiHBG052392.
    InParanoidiO54782.
    KOiK12312.
    OMAiMRAYAAN.
    OrthoDBiEOG7RFTGG.
    PhylomeDBiO54782.
    TreeFamiTF332447.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54782-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPLRWLPLL GQLLLLWPRA AQPAGPIRAF VVPHSHMDVG WVFTVQESMR     50
    AYAANVYTTV VAELVRGGQR RFIAVEQEFF RLWWDGVASE QQKQQVRQLL 100
    HEGRLEFVLG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV 150
    DPFGASATTP TLFALAGFNA HLISRIDYDL KDAMQEAQML QFVWHGSPSL 200
    SGQQEIFTHV MDHYSYCTPS HIPFSNRSGF YWNGVAVFPE PPPDGVYPNM 250
    SEPVTGANIH LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFD 300
    NMDPLLDYIN QRTAQFGISV QYATLNDYFQ ALHATNMTWG IRDHQDFLPY 350
    SSEPLQAWTG FYTSRSTLKG LARQASALLY AGESMFTRYM WPDPSGTLDP 400
    TWALQQLQQL RWAVSEVQHH DAITGTESPK VKNMYTEHLR MGMLGVRKLM 450
    VSIALGGPPG SGTGAPKDIM GPQVTPVLSV DTRPVGYSAS VYNPLAWKIT 500
    TIITLTVAFP NVSVTDELGH PVSTQIQNST KDPSAYDLLI LTTIPGLNYR 550
    HYQVMHARGD QAGTRELVAP RANTLKFSLK LRNQPSQEGK RLVPVMNDCY 600
    ILLFDQDTNM LHSIQDRQSN RTVRMTQEFL EYQANWDVKQ GPISDNYLFA 650
    PNNTAEPSWE AVGMEMVAGT LVTDIRQYFY RYITDQEYIY SIHTRLAHPS 700
    LAGELLCQRI EQQYRVGPLD LNREAILRTS SDLNSQQVLY SDNNGYQMQR 750
    RPYKAFKSNP IPRNYYPMVQ SAFIEDDKSR LVLLAERPHG VSSQGNGQVE 800
    VMLHRRLWNN LAWDLKYNLT LNDTSIVHPV LWLMLGPKST MTALHPRSGV 850
    ALQHGPVVLL KELADEETPV HGPHNPWPVT LPPNLHLQIL SVPGWTYSRS 900
    HAQHLRNLQR GHPEKPQANL QRVLLRLRHL YEAGEDPVLS RPATVDLKVV 950
    LRGLGSVVAV EERSLTGTWD VQMLQRWHWS TKTDHLKGHP TSPPRPPGGS 1000
    IITVYPKEIR TFFIKFQQ 1018
    Length:1,018
    Mass (Da):115,610
    Last modified:June 7, 2005 - v2
    Checksum:i41AD7AAEA7CC88A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti201 – 2011S → T in BAA24266. (PubMed:9425289)Curated
    Sequence conflicti337 – 3371M → I in BAB23655. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006458 mRNA. Translation: BAA24266.1.
    AB083124 Genomic DNA. Translation: BAC53862.1.
    AK004900 mRNA. Translation: BAB23655.1.
    AK040388 mRNA. Translation: BAC30580.1.
    BC066211 mRNA. Translation: AAH66211.1.
    AK173067 mRNA. Translation: BAD32345.1.
    CCDSiCCDS19243.1.
    PIRiJC5799.
    RefSeqiNP_032576.2. NM_008550.2.
    UniGeneiMm.761.

    Genome annotation databases

    EnsembliENSMUST00000031002; ENSMUSP00000031002; ENSMUSG00000029119.
    GeneIDi17160.
    KEGGimmu:17160.
    UCSCiuc008xfb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006458 mRNA. Translation: BAA24266.1 .
    AB083124 Genomic DNA. Translation: BAC53862.1 .
    AK004900 mRNA. Translation: BAB23655.1 .
    AK040388 mRNA. Translation: BAC30580.1 .
    BC066211 mRNA. Translation: AAH66211.1 .
    AK173067 mRNA. Translation: BAD32345.1 .
    CCDSi CCDS19243.1.
    PIRi JC5799.
    RefSeqi NP_032576.2. NM_008550.2.
    UniGenei Mm.761.

    3D structure databases

    ProteinModelPortali O54782.
    SMRi O54782. Positions 27-807.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O54782. 1 interaction.
    MINTi MINT-4100949.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    PTM databases

    PhosphoSitei O54782.

    Proteomic databases

    MaxQBi O54782.
    PaxDbi O54782.
    PRIDEi O54782.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031002 ; ENSMUSP00000031002 ; ENSMUSG00000029119 .
    GeneIDi 17160.
    KEGGi mmu:17160.
    UCSCi uc008xfb.2. mouse.

    Organism-specific databases

    CTDi 23324.
    MGIi MGI:1195262. Man2b2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG306356.
    GeneTreei ENSGT00510000046304.
    HOGENOMi HOG000113450.
    HOVERGENi HBG052392.
    InParanoidi O54782.
    KOi K12312.
    OMAi MRAYAAN.
    OrthoDBi EOG7RFTGG.
    PhylomeDBi O54782.
    TreeFami TF332447.

    Miscellaneous databases

    ChiTaRSi MAN2B2. mouse.
    NextBioi 291434.
    PROi O54782.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O54782.
    Bgeei O54782.
    CleanExi MM_MAN2B2.
    Genevestigatori O54782.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia."
      Hiramoto S., Tamba M., Kiuchi S., Jin Y.-Z., Bannai S., Sugita Y., Dacheux F., Dacheux J.-L., Yoshida M., Okamura N.
      Biochem. Biophys. Res. Commun. 241:439-445(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ddY.
      Tissue: Testis.
    2. "Locarization of a mouse homologue of the porcine 135kDa a-D-mannosidase, mMAN2B2, in acrosome and its involvement in the sperm-egg interaction."
      Hiramoto S., Noguchi J., Endoh Y., Tamba M., Okamura N.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CD-1.
      Tissue: Neural stem cell.
    5. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 763-1018.
      Tissue: Embryonic tail.

    Entry informationi

    Entry nameiMA2B2_MOUSE
    AccessioniPrimary (citable) accession number: O54782
    Secondary accession number(s): Q69ZV1, Q8BH85, Q9DBK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3