SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O54782

- MA2B2_MOUSE

UniProt

O54782 - MA2B2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Epididymis-specific alpha-mannosidase

Gene
Man2b2, Kiaa0935
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays an important role in the early step of spermatogenesis.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc By similarity
Metal bindingi38 – 381Zinc By similarity
Active sitei151 – 1511Nucleophile By similarity
Metal bindingi151 – 1511Zinc By similarity
Metal bindingi420 – 4201Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:Man2b2
Synonyms:Kiaa0935
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1195262. Man2b2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. lysosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 1018997Epididymis-specific alpha-mannosidasePRO_0000012078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi226 – 2261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi249 – 2491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi294 – 2941N-linked (GlcNAc...) Reviewed prediction
Glycosylationi336 – 3361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi511 – 5111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi528 – 5281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi620 – 6201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi652 – 6521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi818 – 8181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi822 – 8221N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO54782.
PaxDbiO54782.
PRIDEiO54782.

PTM databases

PhosphoSiteiO54782.

Expressioni

Tissue specificityi

Mainly expressed in the narrow region between the caput and corpus epididymis.

Developmental stagei

Specifically expressed in type A spermatogonia at stages IX-XI of spermatogenesis. Detected there until the cell developed into type B spermatogonia.

Gene expression databases

ArrayExpressiO54782.
BgeeiO54782.
CleanExiMM_MAN2B2.
GenevestigatoriO54782.

Interactioni

Protein-protein interaction databases

IntActiO54782. 1 interaction.
MINTiMINT-4100949.

Structurei

3D structure databases

ProteinModelPortaliO54782.
SMRiO54782. Positions 27-807.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG306356.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000113450.
HOVERGENiHBG052392.
InParanoidiO54782.
KOiK12312.
OMAiMRAYAAN.
OrthoDBiEOG7RFTGG.
PhylomeDBiO54782.
TreeFamiTF332447.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54782-1 [UniParc]FASTAAdd to Basket

« Hide

MGPLRWLPLL GQLLLLWPRA AQPAGPIRAF VVPHSHMDVG WVFTVQESMR     50
AYAANVYTTV VAELVRGGQR RFIAVEQEFF RLWWDGVASE QQKQQVRQLL 100
HEGRLEFVLG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV 150
DPFGASATTP TLFALAGFNA HLISRIDYDL KDAMQEAQML QFVWHGSPSL 200
SGQQEIFTHV MDHYSYCTPS HIPFSNRSGF YWNGVAVFPE PPPDGVYPNM 250
SEPVTGANIH LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFD 300
NMDPLLDYIN QRTAQFGISV QYATLNDYFQ ALHATNMTWG IRDHQDFLPY 350
SSEPLQAWTG FYTSRSTLKG LARQASALLY AGESMFTRYM WPDPSGTLDP 400
TWALQQLQQL RWAVSEVQHH DAITGTESPK VKNMYTEHLR MGMLGVRKLM 450
VSIALGGPPG SGTGAPKDIM GPQVTPVLSV DTRPVGYSAS VYNPLAWKIT 500
TIITLTVAFP NVSVTDELGH PVSTQIQNST KDPSAYDLLI LTTIPGLNYR 550
HYQVMHARGD QAGTRELVAP RANTLKFSLK LRNQPSQEGK RLVPVMNDCY 600
ILLFDQDTNM LHSIQDRQSN RTVRMTQEFL EYQANWDVKQ GPISDNYLFA 650
PNNTAEPSWE AVGMEMVAGT LVTDIRQYFY RYITDQEYIY SIHTRLAHPS 700
LAGELLCQRI EQQYRVGPLD LNREAILRTS SDLNSQQVLY SDNNGYQMQR 750
RPYKAFKSNP IPRNYYPMVQ SAFIEDDKSR LVLLAERPHG VSSQGNGQVE 800
VMLHRRLWNN LAWDLKYNLT LNDTSIVHPV LWLMLGPKST MTALHPRSGV 850
ALQHGPVVLL KELADEETPV HGPHNPWPVT LPPNLHLQIL SVPGWTYSRS 900
HAQHLRNLQR GHPEKPQANL QRVLLRLRHL YEAGEDPVLS RPATVDLKVV 950
LRGLGSVVAV EERSLTGTWD VQMLQRWHWS TKTDHLKGHP TSPPRPPGGS 1000
IITVYPKEIR TFFIKFQQ 1018
Length:1,018
Mass (Da):115,610
Last modified:June 7, 2005 - v2
Checksum:i41AD7AAEA7CC88A9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011S → T in BAA24266. 1 Publication
Sequence conflicti337 – 3371M → I in BAB23655. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006458 mRNA. Translation: BAA24266.1.
AB083124 Genomic DNA. Translation: BAC53862.1.
AK004900 mRNA. Translation: BAB23655.1.
AK040388 mRNA. Translation: BAC30580.1.
BC066211 mRNA. Translation: AAH66211.1.
AK173067 mRNA. Translation: BAD32345.1.
CCDSiCCDS19243.1.
PIRiJC5799.
RefSeqiNP_032576.2. NM_008550.2.
UniGeneiMm.761.

Genome annotation databases

EnsembliENSMUST00000031002; ENSMUSP00000031002; ENSMUSG00000029119.
GeneIDi17160.
KEGGimmu:17160.
UCSCiuc008xfb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006458 mRNA. Translation: BAA24266.1 .
AB083124 Genomic DNA. Translation: BAC53862.1 .
AK004900 mRNA. Translation: BAB23655.1 .
AK040388 mRNA. Translation: BAC30580.1 .
BC066211 mRNA. Translation: AAH66211.1 .
AK173067 mRNA. Translation: BAD32345.1 .
CCDSi CCDS19243.1.
PIRi JC5799.
RefSeqi NP_032576.2. NM_008550.2.
UniGenei Mm.761.

3D structure databases

ProteinModelPortali O54782.
SMRi O54782. Positions 27-807.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O54782. 1 interaction.
MINTi MINT-4100949.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSitei O54782.

Proteomic databases

MaxQBi O54782.
PaxDbi O54782.
PRIDEi O54782.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031002 ; ENSMUSP00000031002 ; ENSMUSG00000029119 .
GeneIDi 17160.
KEGGi mmu:17160.
UCSCi uc008xfb.2. mouse.

Organism-specific databases

CTDi 23324.
MGIi MGI:1195262. Man2b2.
Rougei Search...

Phylogenomic databases

eggNOGi NOG306356.
GeneTreei ENSGT00510000046304.
HOGENOMi HOG000113450.
HOVERGENi HBG052392.
InParanoidi O54782.
KOi K12312.
OMAi MRAYAAN.
OrthoDBi EOG7RFTGG.
PhylomeDBi O54782.
TreeFami TF332447.

Miscellaneous databases

ChiTaRSi MAN2B2. mouse.
NextBioi 291434.
PROi O54782.
SOURCEi Search...

Gene expression databases

ArrayExpressi O54782.
Bgeei O54782.
CleanExi MM_MAN2B2.
Genevestigatori O54782.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia."
    Hiramoto S., Tamba M., Kiuchi S., Jin Y.-Z., Bannai S., Sugita Y., Dacheux F., Dacheux J.-L., Yoshida M., Okamura N.
    Biochem. Biophys. Res. Commun. 241:439-445(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Testis.
  2. "Locarization of a mouse homologue of the porcine 135kDa a-D-mannosidase, mMAN2B2, in acrosome and its involvement in the sperm-egg interaction."
    Hiramoto S., Noguchi J., Endoh Y., Tamba M., Okamura N.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CD-1.
    Tissue: Neural stem cell.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 763-1018.
    Tissue: Embryonic tail.

Entry informationi

Entry nameiMA2B2_MOUSE
AccessioniPrimary (citable) accession number: O54782
Secondary accession number(s): Q69ZV1, Q8BH85, Q9DBK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 7, 2005
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi