Reviewed, UniProtKB/Swiss-Prot O54781 (SRPK2_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SRPK2
    EC=2.7.11.1
Alternative name(s):
    Serine/arginine-rich protein-specific kinase 2
      Short name=SR-protein-specific kinase 2
    SFRS protein kinase 2

Gene names

Name:

Srpk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. Role in spliceosome assembly and in mediating the trafficking of splicing factors. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1
Cofactor	Magnesium. Ref.1
Enzyme regulation	Activated by phosphorylation on Ser-50 and Ser-581 By similarity. UniProtKB Q96SB4
Subcellular location	Cytoplasm. Nucleus. Ref.1
Tissue specificity	Expressed in testes, lung and brain. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Differentiation mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB protein kinase cascade Ref.1 Inferred from direct assay. Source: UniProtKB spliceosome assembly Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: UniProtKB magnesium ion binding Ref.1 Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SFRS1	Q07955	2	EBI-593325,EBI-398920	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	Serine/threonine-protein kinase SRPK2		PRO_0000086678
Regions
	Domain	79 – 681	603	Protein kinase
	Nucleotide binding	85 – 93	9	ATP By similarity UniProtKB Q9UPE1
Sites
	Active site	212	1	Proton acceptor By similarity UniProtKB Q9UPE1
	Binding site	108	1	ATP By similarity UniProtKB Q9UPE1
Amino acid modifications
	Modified residue	50	1	Phosphoserine; by CK2 By similarity UniProtKB Q96SB4
	Modified residue	317	1	Phosphotyrosine By similarity
	Modified residue	378	1	Phosphoserine By similarity
	Modified residue	487	1	Phosphoserine By similarity
	Modified residue	490	1	Phosphoserine By similarity
	Modified residue	540	1	Phosphothreonine By similarity
	Modified residue	581	1	Phosphoserine; by CK2 By similarity UniProtKB Q96SB4
Experimental info
	Sequence conflict	313	1	Q → QQ in AAH20178. Ref.2
	Sequence conflict	368	1	L → P in BAA24055. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54781-1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 1857EA955B2F9C0E	FASTA	681	76,757
	10 20 30 40 50 60 MSVNSEKSSS SERPEPQQKA PLVPPPPPPP PPPPLPDPAP PEPEEEILGS DDEEQEDPAD 70 80 90 100 110 120 YCKGGYHPVK IGDLFNGRYH VIRKLGWGHF STVWLCWDMQ GKRFVAMKVV KSAQHYTETA 130 140 150 160 170 180 LDEIKLLKCV RESDPSDPNK DMVVQLIDDF KISGMNGIHV CMVFEVLGHH LLKWIIKSNY 190 200 210 220 230 240 QGLPVRCVKS IIRQVLQGLD YLHSKCKIIH TDIKPENILM CVDDAYVRRM AAEATEWQKA 250 260 270 280 290 300 GAPPPSGSAV STAPQQKPIG KISKNKKKKL KKKQKRQAEL LEKRLQEIEE LEREAERKIL 310 320 330 340 350 360 EENITSAEAS GEQDGEYQPE VTLKAADLED TTEEETAKDN GEVEDQEEKE DAEKENAEKD 370 380 390 400 410 420 EDDVEQELAN LDPTWVESPK ANGHIENGPF SLEQQLEDEE DDEDDCANPE EYNLDEPNAE 430 440 450 460 470 480 SDYTYSSSYE QFNGELPNGQ HKTSEFPTPL FSGPLEPVAC GSVISEGSPL TEQEESSPSH 490 500 510 520 530 540 DRSRTVSASS TGDLPKTKTR AADLLVNPLD PRNADKIRVK IADLGNACWV HKHFTEDIQT 550 560 570 580 590 600 RQYRSIEVLI GAGYSTPADI WSTACMAFEL ATGDYLFEPH SGEDYSRDED HIAHIIELLG 610 620 630 640 650 660 SIPRHFALSG KYSREFFNRR GELRHITKLK PWSLFDVLVE KYGWPHEDAA QFTDFLIPML 670 680 EMVPEKRASA GECLRHPWLN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles." Kuroyanagi N., Onogi H., Wakabayashi T., Hagiwara M. Biochem. Biophys. Res. Commun. 242:357-364(1998) [PubMed: 9446799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006036 mRNA. Translation: BAA24055.1. BC020178 mRNA. Translation: AAH20178.1.
IPI	IPI00751009.
PIR	JC5929.
RefSeq	NP_033300.2.
UniGene	Mm.288728
3D structure databases
HSSP	HSSP built from PDB template 1HOW based on UniProtKB Q03656.
SMR	O54781. Positions 64-281, 501-681.
ModBase	Search...
Protein-protein interaction databases
IntAct	O54781. 1 interaction.
PTM databases
PhosphoSite	O54781.
Genome annotation databases
Ensembl	ENSMUSG00000062604. Mus musculus. [Contig view]
GeneID	20817.
KEGG	mmu:20817.
Organism-specific databases
MGI	MGI:1201408. Srpk2.
Phylogenomic databases
HOGENOM	O54781.
HOVERGEN	O54781.
Enzyme and pathway databases
BRENDA	2.7.11.1. 244.
Gene expression databases
ArrayExpress	O54781.
Bgee	O54781.
CleanEx	MM_SRPK2.
GermOnline	ENSMUSG00000062604. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	299545.
SOURCE	Search...

Entry information

Entry name

SRPK2_MOUSE

Accession

Primary (citable) accession number: O54781
Secondary accession number(s): Q8VCD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	May 5, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)