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Protein

WNT1-inducible-signaling pathway protein 1

Gene

Wisp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo.By similarity

GO - Molecular functioni

  1. heparin binding Source: GO_Central
  2. integrin binding Source: GO_Central

GO - Biological processi

  1. cell adhesion Source: GO_Central
  2. cell-cell signaling Source: GO_Central
  3. negative regulation of cell death Source: GO_Central
  4. regulation of cell growth Source: InterPro
  5. signal transduction Source: GO_Central
  6. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
WNT1-inducible-signaling pathway protein 1
Short name:
WISP-1
Alternative name(s):
CCN family member 4
ELM-1
Gene namesi
Name:Wisp1
Synonyms:Ccn4, Elm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1197008. Wisp1.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 367345WNT1-inducible-signaling pathway protein 1PRO_0000014407Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
Glycosylationi143 – 1431N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi273 ↔ 310By similarity
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi290 ↔ 324By similarity
Disulfide bondi301 ↔ 340By similarity
Disulfide bondi304 ↔ 342By similarity
Disulfide bondi309 ↔ 346By similarity
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO54775.

PTM databases

PhosphoSiteiO54775.

Expressioni

Tissue specificityi

Highly expressed in kidney and lung. Lower levels in heart, brain, spleen, liver, skeletal muscle and testis. Expressed in low metastatic melanoma cells.

Gene expression databases

BgeeiO54775.
CleanExiMM_WISP1.
ExpressionAtlasiO54775. baseline and differential.
GenevestigatoriO54775.

Structurei

3D structure databases

ProteinModelPortaliO54775.
SMRiO54775. Positions 59-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 11874IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini121 – 18666VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini215 – 26046TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 34775CTCKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG76761.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO54775.
OMAiVDITKHI.
OrthoDBiEOG7N8ZXB.
PhylomeDBiO54775.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWLLPWTLA AVAVLRVGNI LATALSPTPT TMTFTPAPLE ETTTRPEFCK
60 70 80 90 100
WPCECPQSPP RCPLGVSLIT DGCECCKICA QQLGDNCTEA AICDPHRGLY
110 120 130 140 150
CDYSGDRPRY AIGVCAQVVG VGCVLDGVRY TNGESFQPNC RYNCTCIDGT
160 170 180 190 200
VGCTPLCLSP RPPRLWCRQP RHVRVPGQCC EQWVCDDDAR RPRQTALLDT
210 220 230 240 250
RAFAASGAVE QRYENCIAYT SPWSPCSTTC GLGISTRISN VNARCWPEQE
260 270 280 290 300
SRLCNLRPCD VDIQLHIKAG KKCLAVYQPE EATNFTLAGC VSTRTYRPKY
310 320 330 340 350
CGVCTDNRCC IPYKSKTISV DFQCPEGPGF SRQVLWINAC FCNLSCRNPN
360
DIFADLESYP DFEEIAN
Length:367
Mass (Da):40,703
Last modified:June 1, 1998 - v1
Checksum:i3B7C0569EFAB5E96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791C → R in AAH52677 (PubMed:15489334).Curated
Sequence conflicti199 – 1991D → G in AAH52677 (PubMed:15489334).Curated
Sequence conflicti316 – 3161K → R in AAH52677 (PubMed:15489334).Curated
Sequence conflicti363 – 3631E → G in AAH52677 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004873 mRNA. Translation: BAA24949.1.
AF100777 mRNA. Translation: AAC96319.1.
BC048791 mRNA. Translation: AAH48791.1.
BC052677 mRNA. Translation: AAH52677.1.
CCDSiCCDS27510.1.
RefSeqiNP_061353.1. NM_018865.2.
UniGeneiMm.10222.

Genome annotation databases

EnsembliENSMUST00000005255; ENSMUSP00000005255; ENSMUSG00000005124.
GeneIDi22402.
KEGGimmu:22402.
UCSCiuc007wau.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004873 mRNA. Translation: BAA24949.1.
AF100777 mRNA. Translation: AAC96319.1.
BC048791 mRNA. Translation: AAH48791.1.
BC052677 mRNA. Translation: AAH52677.1.
CCDSiCCDS27510.1.
RefSeqiNP_061353.1. NM_018865.2.
UniGeneiMm.10222.

3D structure databases

ProteinModelPortaliO54775.
SMRiO54775. Positions 59-156.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO54775.

Proteomic databases

PRIDEiO54775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005255; ENSMUSP00000005255; ENSMUSG00000005124.
GeneIDi22402.
KEGGimmu:22402.
UCSCiuc007wau.1. mouse.

Organism-specific databases

CTDi8840.
MGIiMGI:1197008. Wisp1.

Phylogenomic databases

eggNOGiNOG76761.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO54775.
OMAiVDITKHI.
OrthoDBiEOG7N8ZXB.
PhylomeDBiO54775.
TreeFamiTF326070.

Miscellaneous databases

ChiTaRSiWisp1. mouse.
NextBioi302781.
PROiO54775.
SOURCEiSearch...

Gene expression databases

BgeeiO54775.
CleanExiMM_WISP1.
ExpressionAtlasiO54775. baseline and differential.
GenevestigatoriO54775.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the Elm1 gene, a novel gene of the CCN (connective tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed gene) family, suppresses In vivo tumor growth and metastasis of K-1735 murine melanoma cells."
    Hashimoto Y., Shindo-Okada N., Tani M., Nagamachi Y., Takeuchi K., Shiroishi T., Toma H., Yokota J.
    J. Exp. Med. 187:289-296(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: HeN.
  2. "WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors."
    Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C3H/HeN and FVB/N.
    Tissue: Colon and Mesenchymal cell.

Entry informationi

Entry nameiWISP1_MOUSE
AccessioniPrimary (citable) accession number: O54775
Secondary accession number(s): Q80ZL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 1998
Last modified: March 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.