Reviewed, UniProtKB/Swiss-Prot O54775 (WISP1_MOUSE)

Names and origin

Protein names

Recommended name:
    WNT1-inducible-signaling pathway protein 1
      Short name=WISP-1
Alternative name(s):
    ELM-1

Gene names

Name:	Wisp1
Synonyms:	Ccn4, Elm1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Downstream regulator in the Wnt/Frizzled-signaling pathway By similarity. Associated with cell survival. Adheres to skin and melanoma fibroblasts By similarity. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan By similarity. Suppresses tumor growth in vivo.
Subcellular location	Secreted By similarity.
Tissue specificity	Highly expressed in kidney and lung. Lower levels in heart, brain, spleen, liver, skeletal muscle and testis. Expressed in low metastatic melanoma cells.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion Wnt signaling pathway
Cellular component	Secreted
Disease	Proto-oncogene
Domain	Signal
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	insulin-like growth factor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 367	345	WNT1-inducible-signaling pathway protein 1		PRO_0000014407
Regions
	Domain	45 – 118	74	IGFBP N-terminal
	Domain	121 – 186	66	VWFC
	Domain	215 – 260	46	TSP type-1
	Domain	273 – 347	75	CTCK
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Disulfide bond	273 ↔ 310		By similarity
	Disulfide bond	290 ↔ 324		By similarity
	Disulfide bond	301 ↔ 340		By similarity
	Disulfide bond	304 ↔ 342		By similarity
	Disulfide bond	309 ↔ 346		By similarity
Experimental info
	Sequence conflict	79	1	C → R in AAH52677. Ref.3
	Sequence conflict	199	1	D → G in AAH52677. Ref.3
	Sequence conflict	316	1	K → R in AAH52677. Ref.3
	Sequence conflict	363	1	E → G in AAH52677. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O54775-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 3B7C0569EFAB5E96	FASTA	367	40,703
	10 20 30 40 50 60 MRWLLPWTLA AVAVLRVGNI LATALSPTPT TMTFTPAPLE ETTTRPEFCK WPCECPQSPP 70 80 90 100 110 120 RCPLGVSLIT DGCECCKICA QQLGDNCTEA AICDPHRGLY CDYSGDRPRY AIGVCAQVVG 130 140 150 160 170 180 VGCVLDGVRY TNGESFQPNC RYNCTCIDGT VGCTPLCLSP RPPRLWCRQP RHVRVPGQCC 190 200 210 220 230 240 EQWVCDDDAR RPRQTALLDT RAFAASGAVE QRYENCIAYT SPWSPCSTTC GLGISTRISN 250 260 270 280 290 300 VNARCWPEQE SRLCNLRPCD VDIQLHIKAG KKCLAVYQPE EATNFTLAGC VSTRTYRPKY 310 320 330 340 350 360 CGVCTDNRCC IPYKSKTISV DFQCPEGPGF SRQVLWINAC FCNLSCRNPN DIFADLESYP DFEEIAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of the Elm1 gene, a novel gene of the CCN (connective tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed gene) family, suppresses In vivo tumor growth and metastasis of K-1735 murine melanoma cells." Hashimoto Y., Shindo-Okada N., Tani M., Nagamachi Y., Takeuchi K., Shiroishi T., Toma H., Yokota J. J. Exp. Med. 187:289-296(1998) [PubMed: 9449709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: HeN.
[2]	"WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors." Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J. Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed: 9843955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C3H/HeN and FVB/N. Tissue: Colon and Mesenchymal cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB004873 mRNA. Translation: BAA24949.1. AF100777 mRNA. Translation: AAC96319.1. BC048791 mRNA. Translation: AAH48791.1. BC052677 mRNA. Translation: AAH52677.1.
IPI	IPI00117813.
RefSeq	NP_061353.1.
UniGene	Mm.10222
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000005124. Mus musculus. [Contig view]
GeneID	22402.
KEGG	mmu:22402.
Organism-specific databases
MGI	MGI:1197008. Wisp1.
Phylogenomic databases
HOGENOM	O54775.
HOVERGEN	O54775.
OMA	O54775. PRYAIGV.
Gene expression databases
ArrayExpress	O54775.
Bgee	O54775.
CleanEx	MM_WISP1.
GermOnline	ENSMUSG00000005124. Mus musculus.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_growth_fac-bd_prot_N. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302781.
SOURCE	Search...

Entry information

Entry name

WISP1_MOUSE

Accession

Primary (citable) accession number: O54775
Secondary accession number(s): Q80ZL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)