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O54775 (WISP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WNT1-inducible-signaling pathway protein 1

Short name=WISP-1
Alternative name(s):
CCN family member 4
ELM-1
Gene names
Name:Wisp1
Synonyms:Ccn4, Elm1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Downstream regulator in the Wnt/Frizzled-signaling pathway By similarity. Associated with cell survival. Adheres to skin and melanoma fibroblasts By similarity. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan By similarity. Suppresses tumor growth in vivo.

Subcellular location

Secreted By similarity.

Tissue specificity

Highly expressed in kidney and lung. Lower levels in heart, brain, spleen, liver, skeletal muscle and testis. Expressed in low metastatic melanoma cells.

Sequence similarities

Belongs to the CCN family.

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 1 IGFBP N-terminal domain.

Contains 1 TSP type-1 domain.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
Wnt signaling pathway
   Cellular componentSecreted
   DiseaseProto-oncogene
   DomainSignal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell growth

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 367345WNT1-inducible-signaling pathway protein 1
PRO_0000014407

Regions

Domain45 – 11874IGFBP N-terminal
Domain121 – 18666VWFC
Domain215 – 26046TSP type-1
Domain273 – 34775CTCK

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential
Glycosylation3431N-linked (GlcNAc...) Potential
Disulfide bond273 ↔ 310 By similarity
Disulfide bond290 ↔ 324 By similarity
Disulfide bond301 ↔ 340 By similarity
Disulfide bond304 ↔ 342 By similarity
Disulfide bond309 ↔ 346 By similarity

Experimental info

Sequence conflict791C → R in AAH52677. Ref.3
Sequence conflict1991D → G in AAH52677. Ref.3
Sequence conflict3161K → R in AAH52677. Ref.3
Sequence conflict3631E → G in AAH52677. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O54775 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 3B7C0569EFAB5E96

FASTA36740,703
        10         20         30         40         50         60 
MRWLLPWTLA AVAVLRVGNI LATALSPTPT TMTFTPAPLE ETTTRPEFCK WPCECPQSPP 

        70         80         90        100        110        120 
RCPLGVSLIT DGCECCKICA QQLGDNCTEA AICDPHRGLY CDYSGDRPRY AIGVCAQVVG 

       130        140        150        160        170        180 
VGCVLDGVRY TNGESFQPNC RYNCTCIDGT VGCTPLCLSP RPPRLWCRQP RHVRVPGQCC 

       190        200        210        220        230        240 
EQWVCDDDAR RPRQTALLDT RAFAASGAVE QRYENCIAYT SPWSPCSTTC GLGISTRISN 

       250        260        270        280        290        300 
VNARCWPEQE SRLCNLRPCD VDIQLHIKAG KKCLAVYQPE EATNFTLAGC VSTRTYRPKY 

       310        320        330        340        350        360 
CGVCTDNRCC IPYKSKTISV DFQCPEGPGF SRQVLWINAC FCNLSCRNPN DIFADLESYP 


DFEEIAN 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the Elm1 gene, a novel gene of the CCN (connective tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed gene) family, suppresses In vivo tumor growth and metastasis of K-1735 murine melanoma cells."
Hashimoto Y., Shindo-Okada N., Tani M., Nagamachi Y., Takeuchi K., Shiroishi T., Toma H., Yokota J.
J. Exp. Med. 187:289-296(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: HeN.
[2]"WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors."
Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J.
Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Mammary gland.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C3H/HeN and FVB/N.
Tissue: Colon and Mesenchymal cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB004873 mRNA. Translation: BAA24949.1.
AF100777 mRNA. Translation: AAC96319.1.
BC048791 mRNA. Translation: AAH48791.1.
BC052677 mRNA. Translation: AAH52677.1.
CCDSCCDS27510.1.
RefSeqNP_061353.1. NM_018865.2.
UniGeneMm.10222.

3D structure databases

ProteinModelPortalO54775.
SMRO54775. Positions 282-347.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO54775.

Proteomic databases

PRIDEO54775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005255; ENSMUSP00000005255; ENSMUSG00000005124.
ENSMUST00000147079; ENSMUSP00000117402; ENSMUSG00000005124.
GeneID22402.
KEGGmmu:22402.
UCSCuc007wau.1. mouse.

Organism-specific databases

CTD8840.
MGIMGI:1197008. Wisp1.

Phylogenomic databases

eggNOGNOG76761.
GeneTreeENSGT00750000117442.
HOGENOMHOG000231462.
HOVERGENHBG000635.
InParanoidO54775.
OMAVDITKHI.
OrthoDBEOG7N8ZXB.
PhylomeDBO54775.
TreeFamTF326070.

Gene expression databases

ArrayExpressO54775.
BgeeO54775.
CleanExMM_WISP1.
GenevestigatorO54775.

Family and domain databases

InterProIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSWISP1. mouse.
NextBio302781.
PROO54775.
SOURCESearch...

Entry information

Entry nameWISP1_MOUSE
AccessionPrimary (citable) accession number: O54775
Secondary accession number(s): Q80ZL1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot